• Journal of the Korean Society of Food Science and Nutrition
• /
• v.31 no.2
• /
• pp.204-210
• /
• 2002

The quality characteristics and sensory evaluation of chungkookjang were investigated. The samples were prepared and fermented by the inoculation of Bacillus strains; B. subtilis, B. natto and B. licheniformis as a single starter, and mixed culture of B. natto and B. licheniformis on the industrialized model system. It was shown that microbial growth, protease activity, contents of amino-and ammonia-nitrogen and contents of organic acid were higher in B. subtilis inoculated sample, and were lower in B. licheniformis inoculated one. General quality characteristics of sample inoculated by mixed culture of B. natto and B. licheniformis took a middle position between each B. natto and B. licheniformis inoculated one. Fifty eight species of odor components were identified. Ethanol, 3-methyl-1-butanol, acetic acid, benzaldehyde and alkyl pyrazines were identified in all samples and most of other flavor components were strain specific. The contents of unpleasant smell components, alkyl pyrazines and benzaldehyde, were lower in B. licheniformis inoculated sample. The sensory evaluations showed that chungkookjang manufactured from mixed culture of B. natto and B.licheniformis was most acceptable. Therefore, results indicated that chungkookjang manufactured from mixed culture of B. natto and B. licheniformis induced better sensory quality than that of the control.

• International Commerce and Information Review
• /
• v.5 no.2
• /
• pp.3-17
• /
• 2003

This paper examined a e-Business utility of on-line shopping mall firms. Based on the literature review and cases study, the following results were found. First, in e-business policy, it was found that partnership between government and market are very important to e-business utility. Second, e-CRM of on-line shopping mall firms should be considered web site design based on commodity, cafe based on experience and information shares, and transaction confirm service. Third, B2C e-commerce model should be contained firm-specific force, customer oriented force, social force, and technology force.

• The Microorganisms and Industry
• /
• v.16 no.3
• /
• pp.6-14
• /
• 1990

PRDI DNA polymerase is the smallest member of the family B DNA polymerase (Jung et al., 1987). This DNA polyerase is specified by bacteriophage PRDI which infects a wide variety of gram-negative bacteria(Mindich and Bamford, 1988). Because PRDI is highly amenable to genetic and biochemical manipulation, it is a convenient model system with which to study structure-function relationships of DNA polymerase molecules. To determine the functional roles of the highly conserved regions of the family B DNA polymerases, we have initiated site-directed mutagenesis with PRD1 DNA polymerase, and our results show that mutations at the conserved regions within PRD1 DNA polymerase inactivate polymerase complementing activity and catalytic activity.

• Journal of the Korean Society for Precision Engineering
• /
• v.10 no.1
• /
• pp.89-99
• /
• 1993

A solid modeller was developed in this paper. Representation scheme of modelling is nonmanifold B-rep. While namy B-rep modellers use edges as basic element of modelling, this modeller is triangle-based. New modelling element, split triangle that is composed of 3 pure half-edges and has no information about the adjoining triangles, was defined to enlarge the range of representation. Corresponding algorithms for Boolean set operations were also developed.

• Journal of Life Science
• /
• v.27 no.6
• /
• pp.617-622
• /
• 2017

In this study, we sought to develop an effective cloning system by which human cytochrome $b_5$ (cyt $b_5$) is introduced and expressed in zebrafish. First, the 414 bp human cyt $b_5$ gene was amplified from RNA extracts of HeLa cells using RT-PCR, and the amplicon was subsequently sequenced to confirm that it was intact. Next, cyt $b_5$ was cloned into the pEGFP-N3 vector, which also encodes a fluorescent gene. One-cell stage zebrafish embryos were microinjected with the recombinant vector containing the cyt $b_5$ gene. Fluorescence microscopy confirmed high expression of the fluorescent gene in the injected fry compared to the non-fluorescent control fry. Finally, we extracted RNA from the injected fry and performed RT-PCR to determine whether the human cyt $b_5$ gene is expressed in the transgenic zebrafish. Sequencing analysis further confirmed that the cloned human cyt $b_5$ gene was intact. The transgenic zebrafish model produced in this study will be a useful tool to study therapeutic approaches to cure various diseases related to the deficiency of functional human cyt $b_5$ as well as tools for cloning useful genes in fish.

• Journal of Internet Computing and Services
• /
• v.4 no.5
• /
• pp.11-19
• /
• 2003

Since B2C(Business to Customer) from which e-commerce had originated was replaced by B2B, e-Business has shown fast growth so fa., Recently, e-Procurement by l:n concept is on the development as self-purchase system associated with their own ERP In many conglomerates in the Korean market. However, in order to vitalize e-Biz in the plant industry, we need to set up e-marketplaces where many sellers and buyers can meet each other at the same time, which has become the essential part for success as an expanded business model. In this paper, we expect that the foundation for e-transformation in the plant industry is set up by developing Purchase Process System and related modules as the prerequisite for e-Biz in the plant industry, and this report will provide an exemplary model for e-commerce. The Purchase Process System consists of 1) e-Purchasing Module that manages bidding and contract information based on quotation inquiry, 2) e-Expediting Module that manages information to guarantee the on-time delivery, 3) e-Certification Module that controls user authentification, 4) e-Basic Module that manages the bulletin boards, Q&A, etc.

• Journal of Digital Convergence
• /
• v.16 no.9
• /
• pp.147-157
• /
• 2018

Since Google launched a prediction service for influenza-like illness(ILI), studies on ILI prediction based on web search data have proliferated worldwide. In this regard, this study aims to build short-term predictive models for ILI in Korea using ILI and web search data and measure the performance of the said models. In these proposed ILI predictive models specific to Korea, ILI surveillance data of Korea CDC and Korean web search data of Google and Naver were used along with the ARIMA model. Model 1 used only ILI data. Models 2 and 3 added Google and Naver search data to the data of Model 1, respectively. Model 4 included a common query used in Models 2 and 3 in addition to the data used in Model 1. In the training period, the goodness of fit of all predictive models was higher than 95% ($R^2$). In predictive periods 1 and 2, Model 1 yielded the best predictions (99.98% and 96.94%, respectively). Models 3(a), 4(b), and 4(c) achieved stable predictability higher than 90% in all predictive periods, but their performances were not better than that of Model 1. The proposed models that yielded accurate and stable predictions can be applied to early warning systems for the influenza pandemic in Korea, with supplementary studies on improving their performance.

• KSCE Journal of Civil and Environmental Engineering Research
• /
• v.29 no.3B
• /
• pp.247-257
• /
• 2009

In the present paper, turbulent open-channel flows with alternate vegetated zones are numerically simulated using threedimensional model. The Reynolds-averaged Navier-Stokes Equations are solved with the ${\kappa}-{\varepsilon}$ model. The CFD code developed by Olsen(2004) is used for the present study. For model validation, the partly vegetated channel flows are simulated, and the computed depth-averaged mean velocity and Reynolds stress are compared with measured data in the literature. Comparisons reveal that the present model successfully predicts the mean flow and turbulent structures in vegetated open-channel. However, it is found that the ${\kappa}-{\varepsilon}$ model cannot accurately predict the momentum transfer at the interface between the vegetated zone and the non-vegetated zone. It is because the ${\kappa}-{\varepsilon}$ model is the isotropic turbulence model. Next, the open channel flows with alternate vegetated zones are simulated. The computed mean velocities are compared well with the previously reported measured data. Good agreement between the simulated results and the experimental data was found. Also, the turbulent flows are computed for different densities of vegetation. It is found that the vegetation curves the flow and the meandering flow pattern becomes more obvious with increasing vegetation density. When the vegetation density is 9.97%, the recirculation flows occur at the locations opposite to the vegetation zones. The impacts of vegetation on the flow velocity and the water surface elevation are also investigated.

• International Journal of Ocean System Engineering
• /
• v.3 no.1
• /
• pp.1-9
• /
• 2013

A design procedure for a ship with minimum resistance had been developed using a numerical optimization method called SQP (Sequential Quadratic Programming) combined with computational fluid dynamics (CFD) technique. The frictional resistance coefficient was estimated by the ITTC 1957 model-ship correlation line formula and the wave-making resistance coefficient was evaluated by the potential-flow panel method with the nonlinear free surface boundary conditions. The geometry of the hull surface was represented and modified by B-spline surface modeling technique during the optimization process. The Series 60 ($C_B$=0.60) hull was selected as a parent hull to obtain an optimized hull that produces minimum resistance. The models of the parent and optimized hull forms were tested at calm water condition in order to demonstrate the validity of the proposed methodolgy.

• Journal of Astronomy and Space Sciences
• /
• v.33 no.3
• /
• pp.207-210
• /
• 2016

Under the assumption of the presence of a medium-scale E × B drift vortex of plasma in the daytime midlatitude F region, and using a simplified ionospheric model, we demonstrate that the E × B drift produces noticeable perturbations in the horizontal distribution of the plasma density in the upper F region. The pattern of ion density perturbations shows two separate medium scale domains of enhanced and reduced ion density with respect to the background. The E × B drift does not produce multiple small-scale ion density irregularities through plasma mixing because of the suppression effect of the field-aligned ambipolar plasma diffusion.