• Journal of Physiology & Pathology in Korean Medicine
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• v.34 no.1
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• pp.14-23
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• 2020

This study was conducted to evaluate the efficacy of Cheongawongagam on osteoporosis rat. A total of 35 rats were divided into seven groups; Normal control(SD-Nr), experimental control group(OVX-CTL), positive control group(OVX-17β-E2) and herb extracts group[Eucommia ulmoides(OVX-EU-E), Juglandis semen(OVX-JR-SE), Acanthopanax senticosus(OVX-AS-E) extract and Cheongawongagam extracts(OVX-JAEG-E)]. All control group, and herb extracts group were ovariectomized. After the 3 weeks recovery period, herb extract group were orally administered 200 mg / kg of the EU-E, JR-SE, AS-E and JAEG-E for 12 weeks. In the OVX-CTL, 17β-estradiol(E2) was administered subcutaneously on the back of the rats at a dose of 0.03 ug/sc. Their body weight, serum total cholesterol, triglyceride, aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), Leukotriene B4 (LTB4), calcium (Ca), estradiol, osteocalcin, and deoxypyridinoline (DPD) concentration were measured. Also, we investigated mRNA expression of inflammatory cytokine, MMP-2, MMP-9, and bone tissue. As a result, total cholesterol was significantly decreased in the OVX-AS-E and OVX-JAEG-E. ALP was significantly increased and osteocalcin, DPD was significantly decreased in OVX-JAEG-E. The expression of inflammatory mediators (TNF-α, IL-1β, LTB4, COX-2, NOS-2), inflammatory cytokines IL-1β and MMP-9 mRNA were significantly decreased in OVX-JAEG-E. Histologic examination of the femur showed that bone mineral density, and bone mass were increased and bone marrow were decreased in the OVX-JAEG-E group. The above experiment shows that cheongawongagam extract were effective in the prevention and treatment of osteoporosis.

• Proceedings of the Korea Society of Poultry Science Conference
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• 2002.11a
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• pp.140-142
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• 2002

This study was conducted to obtain some genetic information for developing superior Korean native chickens. Heterosis and combining ability effects were estimated with 1,080 progenies of full diallel crosses of 3 strains in Korean Native Chicken. The traits of egg productivity were Investigated, and heterosis effect, general combining ability, specific combining ability and reciprocal effect were analyzed model I of the Griffing's method 1. The general performance were 147.0 days in age at first egg laying, 71.8 eggs in egg numbers to 270 days, 50.1g in egg weight at 270 days, 75.6 in egg shape index, 356$\mu\textrm{m}$ in egg shell thickness, and 4.27kg/$\textrm{cm}^2$ in egg shell strength, respectively. According to result, heterosis effect revealed comparatively high in age at first egg laying, egg numbers, egg weight, and egg shell strength. The effects estimated highly in general combining ability for age at first egg laying, egg numbers, egg weight, egg shape Index and egg shell thickness, and in specific combining ability were egg numbers, egg weight and egg shell thickness, and in reciprocal effects for egg numbers, egg shape index, and egg shell thickness. This result could be used for developing Korean native chicken.

• Journal of Korean Society of Water and Wastewater
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• v.12 no.2
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• pp.90-98
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• 1998

The optimal rechlorination in water distribution systems was investigated by incorporating optimization techniques into a numerical water quality model. For a hypothetical system that consists of 10 junctions including a storage tank and 12 links, the bulk ($k_b$) and pipe-wall ($k_w$) decay-rate constants of chlorine residual are assumed to be 2.0 1/day and 1.5 m/day, respectively. It was also assumed that the lower and upper limits of chlorine residual in the network are 0.2 mg/L and 0.6 mg/L. When the chlorine source is only the storage tank (without rechlorination), the high levels of chlorine residual appear near the storage tank to maintain the chlorine residuals above the lower limit over the junctions. On the other hand, the chlorine residuals in the network are distribute within the desirable range (0.2 - 0.6 mg/L) after the optimal rechlorination through five injection sites including the storage tank. In case of a real water distribution system that comprises 28 junctions including a clear well and 27 links, the bulk and pipe-wall decay-rate constants are 0.3 1/day and 0.2 m/day, respectively. Before rechlorination, the required chlorine residual at the clearwell is 5.1 mg/L to keep the chlorine residuals above the minimum level (0.6 mg/L) over the junctions. By the optimal rechlorination at five injection sites, the chlorine residuals are distributed within a desirable range of 0.6 mg/L through 2.0 mg/L, which can avoid the excess of chlorine residuals near the clear well. Consequently, total chlirine doses are decreased by 81% in the hypothetical distribution network and 69 % in the real distribution network for satisfying the minimum chlorine residuals.

• Korean Journal of Medicinal Crop Science
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• v.18 no.4
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• pp.224-230
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• 2010

This study was conducted to investigate anti-oxidative and inflammatory inhibition effects of green tea and dietary fiber mixture on liver of high fat diet-induced obese rats. 21 male rats were divided into 3 dietary groups and control group (A), high fat diet-induced group (B), and high fat (HF) diet-induced + EQ diet-$S^{(R)}$ diet group (C). Immunoblotting and RT-PCR analysis revealed protein expression, and anti-oxidant analysis revealed MDA (malondialdehyde), GSH (glutathione), and free DPPH radical. As a results, Body weight and food consumption were not significantly different between groups. The levels of MDA and GSH were lower in HF + $EQS^{(R)}$ group than in HF group. Also, the $EQS^{(R)}$ demonstrated to be more effective than HF group for a DPPH radicals scavenging activities. In addition, protein and mRNA level of TNF-$\alpha$ in HF + $EQS^{(R)}$ group showed relatively more potent pro-inflammatory activity inhibition compared to HF group. These results suggest that green tea mixture (EQ diet-$S^{(R)}$) provide positive effects on anti-oxidative and inflammatory inhibition effects on obese animal model or obesity related diseases.

• Journal of Biosystems Engineering
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• v.5 no.2
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• pp.26-39
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• 1980

A study was investigated to find out the mechanical optimum conditions of power tiller-plow system on both paddy field and upland. Mathematical model was developed for the theoretical analysis of this system and the experimentation on the field was carried out with two different sizes of 5PS and 8PS power tiller equipped with rubber tire. 1) The relationship between the plowing depth and draft resistance of the power tiller-plow system was a quadratic function. 2) The minimum point of the specific draft resistance of the 5 PS plow was found at the smaller plowing depth than that of 8 PS plow, therefore we can find that the curved surface of 5PS plow bottom should be improved for the effective plowing operation. 3) As the improvement of the mechanical balance by the desirable change of the curved surface of plow bottom, the relative position of hitch point and dimension of plow beam would be realized, the 5 PS power tiller could be used to plow deeply (about 16-17cm). 4) The virtual acting point of the total draft resistance on the plow bottom approached to the land side as the plowing depth increased. 5) The resultant of vertical reaction force $R_2$ on the landside was increased with the plowing depth, while the vertical reaction force $R_1$ on the wheel was decreased as the slope angle of the body of power tiller increased. 6) For the effective plowing operations ; a) The slope angle of the body should be as small as possible. b) The diameter of the wheel should be as small possible. c) The horizontal and vertical distances $l_2, h_1$ between the wheel axis and plow bottom should be as large as possible. 7) To use the 5PS power tiller as the major unit of agricultural machinery, the curved surface of the 5 PS plower bottom and the mechanism of attachment between the power tiller and the plow should be changed as the indications of this study, and in addition to these, the new operation method of the field work should be developed.

• Journal of Korean Neurosurgical Society
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• v.53 no.2
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• pp.72-76
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• 2013

Objective : Cerebral aneurysm (CA) is an important acquired cerebrovascular disease that can cause catastrophic results. MicroRNAs (miRNAs) are small non-coding RNAs, playing essential roles in modulating basic physiologic and pathological processes. Currently, evidences have been established about biologic relationship between miRNAs and abdominal aortic aneurysms. However, biologic roles of miRNAs in CA formation have not been explained yet. We employed microarray analysis to detect and compare miRNA expression profiles in late stage of CA in rat model. Methods : Twenty-six, 7-week-old male Sprague-Dawley rats underwent a CA induction procedure. The control animals (n=11) were fed a normal diet, and the experimental animals (n=26) were fed a normal diet with 1% normal saline for 3 months. Then, the rats were sacrificed, their cerebral arteries were dissected, and the five regions of aneurysmal dilation on the left posterior communicating artery were cut for miRNA microarrays analysis. Six miRNAs (miRNA-1, miRNA-223, miRNA-24-1-5p, miRNA-551b, miRNA-433, and miRNA-489) were randomly chosen for validation using real-time quantitative PCR. Results : Among a set of differentially expressed miRNAs, 14 miRNAs were over-expressed more than 200% and 6 miRNAs were down-expressed lower than 50% in the CA tissues. Conclusion : The results show that miRNAs might take part in CA formation probably by affecting multiple target genes and signaling pathways. Further investigations to identify the exact roles of these miRNAs in CA formation are required.

• Journal of the Korean Society of Safety
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• v.31 no.5
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• pp.133-140
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• 2016

Most small weir installed in Korea is concrete solidated weir. Fixed weir causes stagnant flow, which leads to deposit sediment just upstream of weir. As time goes on, it would induce reduction of water storage capacity and invoke the serious water quality issues. Therefore, there has been a growing interest in movable weir. Especially, the flexible rubber weir is easy to install and possible to operate in extreme environments. However, even though this type can be flatable, it is also not free from sediment deposition problem. Thus, to enhance the ability of releasing deposition the bypass pipe was constructed underneath it. In this study the performance of its ability was examined with hydraulic model test. This bypass pipe was designed with 3 different dimensions to connect between each bottom of upstream and downstream of a weir, such as Type A, B, and C. The efficiency of drainage of deposition upstream was studied under two water of upstream and sediment heights. In addition, the ability of sediment emission through the bypass pipe after the pipe was blocked by debris like soil, vegetation et al. was examined by video monitoring. From this study, it was suggested a dimensionless equation which show the relationship of variable parameters and amount of emission sediment through bypass pipe. And it was found that the most significant factors on efficiency of releasing were elbow angle and discharge, and the ability of emission when the pipe was blocked was most highly influenced in tilting length.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1997.04a
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• pp.119-119
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• 1997

Valacyclovir is a L-valyl ester prodrug of acyclovir which is a highly effective and selective antiviral agent in the treatment of herpes virus diseases. Valacyclovir is rapidly and almost completely converted to acyclovir and increases the oral bioavailability of acyclovir three to five fold. However, the intestinal absorption mechanism of valacyclovir is not clear. If the improved absorption mechanism of valacyclovir is fully understood, it will provide a rationale of designing the amino acid ester prodrugs of polar drugs containing hydroxyl group. The main objective of our present study is to characterize the membrane transport mechanism of valacyclovir. Methods : Intestinal absorption of valacyclovir was investigated by using in-situ rat perfusion study and its wall permeability was estimated by modified boundary layer model. The membrane transport mechanism was also investigated through the uptake study in Caco-2 cells and in CHO-hPepTl cells. Results : In the rat perfusion study, the wall permeability of valacyclovir was ten times higher than acyclovir and showed concentration dependency, Valacyclovir also demonstrated a D,L stereo-selectivity with L-isomer having an approximately five-fold higher permeability than D-isomer. Mixed dipeptides and cephalexin, which are transported by dipeptide carriers, strongly competed with valacyclovir for the intestinal absorption, while L-valine did not show any competition with valacyclovir. This indicated that the intestinal absorption of valacyclovir could be dipeptide carrier-mediated. In addition, the competitive uptake study in Caco-2 cells presented that dipeptides reduced the valacyclovir uptake but valine did not. Also, in IC$\sub$50/ study, valacyclovir showed strong inhibition on the $^3$H-gly-sar uptake in CHO-hPepTl cells over-expressing a human intestinal peptide transporter. Taken together, the result from our present study indicated that valacyclovir utilized the peptide transporter for the intestinal absorption.

• Journal of Microbiology and Biotechnology
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• v.28 no.3
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• pp.357-366
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• 2018

Periodontitis, an infective disease caused by oral pathogens and the intrinsic aging process, results in the destruction of periodontal tissues and the loss of alveolar bone. This study investigated whether Boesenbergia pandurata extract (BPE) standardized with panduratin A exerted anti-periodontitis effects, using an aging model representative of naturally occurring periodontitis. In aged rats, the oral administration of BPE ($200mg{\cdot}kg^{-1}{\cdot}day^{-1}$) for 8 weeks significantly reduced the mRNA and protein expression of $interleukin-1{\beta}$, nuclear factor-kappa B, matrix metalloproteinase (MMP)-2, and MMP-8 in gingival tissues (p < 0.01). In alveolar bone, histological analysis with staining and micro-computed tomography revealed the attenuation of alveolar bone resorption in the BPE-treated aged group, which led to a significant reduction in the mRNA and protein expression of nuclear factor of activated T-cells c1 (NFATc1), c-Fos, tartrate-resistant acid phosphatase, and cathepsin K (p < 0.01). BPE not only increased the expression of osteoblast differentiation markers, such as alkaline phosphate, and collagen type I (COL1A1), but also increased the ratio of osteoprotegerin to RANKL. Collectively, the results strongly suggested that BPE is a natural resource for the prevention or treatment of periodontal diseases.

• Magazine of the Korean Society of Agricultural Engineers
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• v.6 no.2
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• pp.816-825
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• 1964

1. Outline: The flow of the siphon spillway is very intricate and affected by vacuous condition. It is an intresting problem to make vacuum rapidly in_siphon, i.e, to provoke the perfect siphonaoge with the lowest water-level of overflow. The hydraulic experiments of siphon spillway have practiced at the Masan Lake, Haenam, the province of Junnam and gained rational and economical results which cannot be odtained by calculation. 2. Experiment: The model scale was a half of that of the prototype. 3. Results and discussion: In the experiments of 14 runs, washing, overflow water level, outlet, water level of cushion, phenomenon of water flying and water pressure of every part, etc. were studied. a) The relation of overflow water-level at siphon inlet and deflector When the elevation of the deflector is 10. 65m and the form of deflector O.4m long is the hypotenuse of an $45^{\circ}$ isosceles triangle, the over flow water-level is the minimum, i. e. the siphonage was excellent. There is no effect by the rising of overflow water-Ievel between 11.95m to 1O.65m of deflector elevation (in the first plan, it is 11.05m). But the overflow water-level rises remarkably in the outside region of the above limits. b) The relation of overflow water-level, the length of cushion and standard height of the base. The reduction of the length of cushion brings the rising of overflow waterlevel, and the rising of the standard height of the base brings the rising of overflow water level. For the long cushion length and low standard height of the base, it cannot be expeted to have the falling of overflow water-level. The most satisfactory data were obtained at 5.20m of the base standard height and 6.1m of the length of cushion. The first vah,le planned was 5.70 m and 4.30m.