• Journal of Applied Biological Chemistry
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• v.43 no.3
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• pp.147-151
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• 2000

A gene, nprM, from Bacillus megaterium ATCC 14945 was obtained by PCR using primers synthesized based on two nprM sequences from two different strains, and cloned into Escherichia coli. The gene nprM encoded an extracellular neutral protease, and the molecular mass of the expressed enzyme was estimated to be approximately 36kDa on a denaturating gel. The enzyme was activated by $Ca^{2+}$, and the optimum concentration of $Ca^{2+}$ was 5 mM. The enzyme was inhibited by EDTA but not by PMSF. The optimal pH and temperature of the cloned enzyme were $50^{\circ}C and pH 7.5-8.0, respectively, and were similar to those of the enzyme from the gene gonor cell. The cloned NprM caused internal cleavage of the native endoglucanase of B. subtilis BSE616 as a model foregin protein, and resulted in a small truncated but still active endoglucanase.

• English Language & Literature Teaching
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• v.16 no.3
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• pp.339-363
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• 2010

The purpose of this paper is to investigate how the English classes in secondary schools in Korea are in terms of communicative nature. After literature study, COLT parts A and B were used to analyze three kinds of English classes: a common class type taught by a school teacher who had won 1st prize at TEE contest, a team teaching class taught by NNS and NS, and a model class taught by a pre-service English teacher. Their communicative English classes were videotaped and transcribed for analysis by COLT. The results of the study revealed that students participated in the classes mostly as a unit and, therefore, did not get many meaningful chances to speak English. Second, creative language-use opportunities were barely found during classes. Third, the group work seemed to be prepared before the class, not to be done during the class. Therefore it is likely to be learned from memorization rather than meaningful communication. These results suggest English classes in schools be more communicatively oriented for successful English learning. Further study should be conducted in order to make English classes more communicative to help students communicate their genuine messages in English and to help teachers teach English efficiently.

• Bulletin of the Korean Chemical Society
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• v.31 no.10
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• pp.2903-2908
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• 2010

Protein S-nitrosation is common in cells under nitrosative stress. In order to model proteins with S-nitrosocysteine (CysSNO) residues, we first developed an Amber force field for S-nitrosoethanethiol (EtSNO) and then transferred it to CysSNO. Partial atomic charges for EtSNO and CysSNO were obtained by a restrained electrostatic potential approach to be compatible with the Amber-99 force field. The force field parameters for bonds and angles in EtSNO were obtained from a generalized Amber force field (GAFF) by running the Antechamber module of the Amber software package. The GAFF parameters for the CC-SN and CS-NO dihedrals were not accurate and thus determined anew. The CC-SN and CS-NO torsional energy profiles of EtSNO were calculated quantum mechanically at the level of B3LYP/cc-pVTZ//HF/6-$31G^*$. Torsional force constants were obtained by fitting the theoretical torsional energies with those obtained from molecular mechanics energy minimization. These parameters for EtSNO reproduced, to a reasonable accuracy, the corresponding torsional energy profiles of the capped tripeptide ACE-CysSNO-NME as well as their structures obtained from quantum mechanical geometry optimization. A molecular dynamics simulation of myoglobin with a CysSNO residue produced a well-behaved trajectory demonstrating that the parameters may be used in modeling other S-nitrosated proteins.

• Structural Engineering and Mechanics
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• v.56 no.6
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• pp.917-938
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• 2015

The nonlinear numerical analysis of the impact response of reinforced concrete/mortar beam incorporated with the updated Lagrangian method, namely the Smoothed Particle Hydrodynamics (SPH) is carried out in this study. The analysis includes the simulation of the effects of high mass low velocity impact load falling on beam structures. Three material models to describe the localized failure of structural elements are: (1) linear pressure-sensitive yield criteria (Drucker-Prager type) in the pre-peak regime for the concrete/mortar meanwhile, the shear strain energy criterion (Von Mises) is applied for the steel reinforcement (2) nonlinear hardening law by means of modified linear Drucker-Prager envelope by employing the plane cap surface to simulate the irreversible plastic behavior of concrete/mortar (3) implementation of linear and nonlinear softening in tension and compression regions, respectively, to express the complex behavior of concrete material during short time loading condition. Validation upon existing experimental test results is conducted, from which the impact behavior of concrete beams are best described using the SPH model adopting an average velocity and erosion algorithm, where instability in terms of numerical fragmentation is reduced considerably.

• Journal of Applied Reliability
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• v.17 no.3
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• pp.224-235
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• 2017

Purpose: Reliability is the most important factor to detect defects as wind turbines are deployed in large blades. The methods of detecting defects are various, such as non-destructive inspection and thermal imaging inspection. We propose the phased array ultrasonic testing method of non-destructive testing. Methods: We propose the active pressure mechanism for wind power blade. The phase array ultrasonic inspection method is used for fault detection inner blade surface. Controlled pressure of mechanism with respect to z-axis is important for guarantee the result of phase array ultrasonic inspection. The model based control and proposed mechanism are utilized for overall system stability and effectiveness of system. Result: The result of proposed pressure mechanism B is more stable than A. Convergence speed is also faster than A. Conclusion: We confirmed the performance of the proposed constant pressure mechanism through experiments. Non-destructive testing was applied to the specimen to confirm the reliability of detecting defects.

• KIEE International Transaction on Electrical Machinery and Energy Conversion Systems
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• v.4B no.3
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• pp.146-152
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• 2004

Cost effective simulation schemes for Wind Power Generation Systems (WPGS) considering wind turbine types, generators and load capacities have been strongly investigated by researchers. As an alternative, a true weather condition based simulation method using a real-time digital simulator (RTDS) is experimented in this paper for the online real-time simulation of the WPGS. A stand-alone WPGS is, especially, simulated using the Simulation method for WPGS using Real Weather conditions (SWRW) in this work. The characteristic equation of a wind turbine is implemented in the RTDS and a RTDS model component that can be used to represent any type of wind turbine in the simulations is also established. The actual data related to weather conditions are interfaced directly to the RTDS for the purpose of online real-time simulation of the stand-alone WPGS. The outcomes of the simulation demonstrate the effectiveness of the proposed simulation scheme. The results also signify that the cost effective verification of efficiency and stability for the WPGS is possible by the proposed real-time simulation method.

• Journal of the Institute of Convergence Signal Processing
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• v.5 no.3
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• pp.210-215
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• 2004

The dynamic model of ac servo motor is influenced very much due to rotor resistance change and nonlinear characteristic. By using the sliding mode control the dynamic behavior of system can be made insensitive to plant parameter change and external disturbance. This paper describes the application of the sliding mode control for position control of ac servo motor. The control scheme is derived and designed. A design method based on external load parameters has been developed for the robust control of ac induction servo drive. The proposed control scheme are given based on the variable structure controller and slip frequency vector control. Simulated results are given to verify the proposed design method by adoption of sliding mode and show robust control for a change of shaft initial J, viscous friction B and torque disturbance.

• The Transactions of the Korean Institute of Power Electronics
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• v.4 no.3
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• pp.223-230
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• 1999

A conventional sliding mode control approach is often impractical or difficult when it is applied to high order process b because the number of tuning parameters in the sliding mode controller increases with the order of the plant. C Camacho(l996) proposed a design method of a fixed structure sliding mode controller based on a first order plus dead t time approximation to the higher-order process. But, his method has such problems as chattering, over‘shoot, and c command following due to the Taylor the approximation en‘ors for the time delay term of the first order model. In this p paper, a new design technique for a sliding mode controller based on the modified Taylor approximation considered a w weight is developed to improve the Camacho's problems.

• International Journal of Quality Innovation
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• v.3 no.2
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• pp.138-158
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• 2002

Nowadays, new product development (NPD) is one of the most crucial factors for business success. The manufacturing firms cannot afford the resources in the long development cycle and the costly redesigns. Good product planning is crucial to ensure the success of NPD, while the Quality Function deployment (QFD) is an effective tool to help the decision makers to determine appropriate product specifications in the product planning stage. Traditionally, in the QFD, the product specifications are determined by a rather subjective evaluation, which is based on the knowledge and experience of the decision makers. In this paper, the traditional QFD methodology is firstly reviewed. An improved Hybrid Quality Function Deployment (HQFD) [MSOfficel] then presented to tackle the shortcomings of traditional QFD methodologies in determining the engineering characteristics. A structured questionnaire to collect and analyze the customer requirements, a methodology to establish a QFD record base and effective case retrieval, and a model to more objectively determine the target values of engineering characteristics are also described.

• Korean Journal of Organic Agriculture
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• v.19 no.spc
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• pp.291-294
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• 2011

We studies a model for management of pepper anthracnose based covering method and spraying system in field. 1. Among 82 organic fungicides, 42 materials showed most effective inhibition against mycelia growth of the Colletotrichum acutatum in vitro. 23 formulated biocontrol agents were chosen to control the disease from 42 biocontrol agents in greenhouse. In the end, five kinds (2 plant extracts, 2 biopesiticides, 1 Bordeaux mixture) were selected from 23 materials in the field. 2. The mulching materials of bed covering in fruit season were thin non-woven fabric sheet and black plastic. The use of a fabric sheet was reduced the spread of anthracnose as compared to the plastic covering. 3. The application with the chosen materials was reduced 34% of anthracnose for 7 times sprays to planting 70 days as compared to the untreated control. In yield, nonwoven fabric sheet with formulated biopesticides was increased 17% than black plastic. 4. This result indicated that the developed biocontrol strategy could be an effective and economic crop protection system in organic pepper cultivation field.