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Physiochemical characteristics and fermentation ability of milk from Czech Fleckvieh cows are related to genetic polymorphisms of β-casein, κ-casein, and β-lactoglobulin

  • Kyselova, Jitka;Jecminkova, Katerina;Matejickova, Jitka;Hanus, Oto;Kott, Tomas;Stipkova, Miloslava;Krejcova, Michaela
    • Asian-Australasian Journal of Animal Sciences
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    • v.32 no.1
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    • pp.14-22
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    • 2019
  • Objective: The aim of the study was to find a possible association between the ${\beta}-$ and ${\kappa}-casein$ and ${\beta}-lactoglobulin$ genotypes and important milk physiochemical and technological characteristics such as acidity, alcohol stability, the contents of some minerals and the parameters of acid fermentation ability (FEA) in Czech Fleckvieh Cattle. Methods: Milk and blood samples were collected from 338 primiparous Czech Fleckvieh cows at the same stage of lactation. The genotypes of individual cows for ${\kappa}-casein$ (alleles A, B, and E) and ${\beta}-lactoglobulin$ (alleles A and B) were ascertained by polymerase chain reaction-restriction fragment length polymorphism, while their ${\beta}-casein$ (alleles $A^1$, $A^2$, $A^3$, and B) genotype was determined using melting curve genotyping analysis. The data collected were i) milk traits including active acidity (pH), titratable acidity (TA), alcohol stability (AS); calcium (Ca), phosphorus (P), sodium (Na), magnesium (Mg), and potassium (K) contents; and ii) yoghurt traits including active acidity (Y-pH), titratable acidity (Y-TA), and the counts of both Lactobacilli and Streptococci in 1 mL of yoghurt. A linear model was assumed with fixed effects of herd, year, and season of calving, an effect of the age of the cow at first calving and effects of the casein and lactoglobulin genotypes of ${\beta}-CN$ (${\beta}-casein$, CSN2), ${\kappa}-CN$ (${\kappa}-casein$, CSN3), and ${\beta}-LG$ (${\beta}-lactoglobulin$, LGB), or the three-way interaction between those genes. Results: The genetic polymorphisms were related to the milk TA, AS, content of P and Ca, Y-pH and Lactobacilli number in the fresh yoghurt. The CSN3 genotype was significantly associated with milk AS (p<0.05). The effect of the composite CSN2-CSN3-LGB genotype on the investigated traits mostly reflected the effects of the individual genes. It significantly influenced TA (p<0.01), Y-pH (p<0.05) and the log of the Lactobacilli count (p<0.05). Conclusion: Our findings indicate that the yoghurt fermentation test together with milk proteins genotyping could contribute to milk quality control and highlight new perspectives in dairy cattle selection.

Acoustic Feedback and Noise Cancellation of Hearing Aids by Deep Learning Algorithm (심층학습 알고리즘을 이용한 보청기의 음향궤환 및 잡음 제거)

  • Lee, Haeng-Woo
    • The Journal of the Korea institute of electronic communication sciences
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    • v.14 no.6
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    • pp.1249-1256
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    • 2019
  • In this paper, we propose a new algorithm to remove acoustic feedback and noise in hearing aids. Instead of using the conventional FIR structure, this algorithm is a deep learning algorithm using neural network adaptive prediction filter to improve the feedback and noise reduction performance. The feedback canceller first removes the feedback signal from the microphone signal and then removes the noise using the Wiener filter technique. Noise elimination is to estimate the speech from the speech signal containing noise using the linear prediction model according to the periodicity of the speech signal. In order to ensure stable convergence of two adaptive systems in a loop, coefficient updates of the feedback canceller and noise canceller are separated and converged using the residual error signal generated after the cancellation. In order to verify the performance of the feedback and noise canceller proposed in this study, a simulation program was written and simulated. Experimental results show that the proposed deep learning algorithm improves the signal to feedback ratio(: SFR) of about 10 dB in the feedback canceller and the signal to noise ratio enhancement(: SNRE) of about 3 dB in the noise canceller than the conventional FIR structure.

Multi-view Video Coding using View Interpolation (영상 보간을 이용한 다시점 비디오 부호화 방법)

  • Lee, Cheon;Oh, Kwan-Jung;Ho, Yo-Sung
    • Journal of Broadcast Engineering
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    • v.12 no.2
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    • pp.128-136
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    • 2007
  • Since the multi-view video is a set of video sequences captured by multiple array cameras for the same three-dimensional scene, it can provide multiple viewpoint images using geometrical manipulation and intermediate view generation. Although multi-view video allows us to experience more realistic feeling with a wide range of images, the amount of data to be processed increases in proportion to the number of cameras. Therefore, we need to develop efficient coding methods. One of the possible approaches to multi-view video coding is to generate an intermediate image using view interpolation method and to use the interpolated image as an additional reference frame. The previous view interpolation method for multi-view video coding employs fixed size block matching over the pre-determined disparity search range. However, if the disparity search range is not proper, disparity error may occur. In this paper, we propose an efficient view interpolation method using initial disparity estimation, variable block-based estimation, and pixel-level estimation using adjusted search ranges. In addition, we propose a multi-view video coding method based on H.264/AVC to exploit the intermediate image. Intermediate images have been improved about $1{\sim}4dB$ using the proposed method compared to the previous view interpolation method, and the coding efficiency have been improved about 0.5 dB compared to the reference model.

Mediating Effect of Coping Strategies in the Relationship between Pain Beliefs and Depression, Pain Disability among Chronic Back Pain Patients (만성요통환자에서의 통증신념과 우울, 통증생활방해와의 관계에서 대처전략의 매개효과)

  • Kim, Kyoung-A;Chu, Sanghui
    • The Journal of the Korea Contents Association
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    • v.17 no.10
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    • pp.206-215
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    • 2017
  • The Purpose of this study was to investigate of coping strategies(active and passive) between pain beliefs and depression, pain disability among chronic back pain patients. Data were analyzed by the SPSS-WIN 21.0 program. Indirect SPSS macro(Bootsrapping)was used to analyze the multiple-mediation model of this study. The result showed that the mean score for pain belief was $3.42{\pm}9.67$, and he passive coping strategies was $29.68{\pm}8.04$, active coping was $25.49{\pm}4.22$. The mean score of depression was $25.49{\pm}11.56$. The pain disability index was $46.94{\pm}12.65$. It found that there were significant correlations among the 5 variables. The multiple mediated effects of passive coping and active coping on pain beliefs and depression were (b=.453, 95% CI=.228, .703) and on pain beliefs and pain disability were (b = .285, 95% CI = .131, .519) in chronic low back pain patients. This study discovered that the active coping strategies had a positive mediating effect in the relationship between pain beliefs and depression, pain beliefs and pain disability. And passive coping strategies had a negative mediating effect. Based on findings of this study, improving the active coping strategy programs or management is highly recommended in chronic back pain patients.

Effects of plant-based Korean food extracts on lipopolysaccharide-stimulated production of inflammatory mediators in vitro

  • Lee, Sun Young;Kim, Yoo-Sun;Lim, Ji Ye;Chang, Namsoo;Kang, Myung-Hee;Oh, Se-Young;Lee, He-Jin;Kim, Hyesook;Kim, Yuri
    • Nutrition Research and Practice
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    • v.8 no.3
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    • pp.249-256
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    • 2014
  • BACKGROUND/OBJECTIVES: The traditional Korean diet is plant-based and rich in antioxidants. Previous studies have investigated the potential health benefits of individual nutrients of Korean foods. However, the cumulative effects of a Korean diet on inflammation remain poorly understood. Therefore, the aim of this study was to investigate the anti-inflammatory effects of a plant-based Korean diet. MATERIALS/METHODS: Using data from the Fifth Korean National Health and Nutrition Examination Survey, 75 individual plant food items were selected which represent over 1% of the total diet intake of the Korean diet. These items were classified into ten different food groups, and the vegetable (Veg) and fruit (Fruit) groups were studied based on their high antioxidant capacity. For comparison, a mixture of all ten groups (Mix) was prepared. To produce a model of inflammation with which to test these Veg, Fruit, and Mix plant-based Korean food extracts (PKE), RAW264.7 macrophages were treated with lipopolysaccharide (LPS). RESULTS: Levels of nitric oxide (NO) and prostaglandin $E_2$ ($PGE_2$), as well as protein expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were found to be lower following PKE treatment. Furthermore, PKE treatment was found to suppress tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) and interleukin-6 (IL-6) via the nuclear transcription factor kappa-B ($NF-{\kappa}B$) signaling pathway. Overall, the Mix group exhibited the greatest anti-inflammatory effects compared with Veg and Fruit PKE group. CONCLUSIONS: Inhibition of LPS-induced pro-inflammatory mediators by the PKE tested was found to involve an inhibition of NF-kB activation. Moreover, PKE tested have the potential to ameliorate various inflammation-related diseases by limiting the excessive production of pro-inflammatory mediators.

Underdetermined blind source separation using normalized spatial covariance matrix and multichannel nonnegative matrix factorization (멀티채널 비음수 행렬분해와 정규화된 공간 공분산 행렬을 이용한 미결정 블라인드 소스 분리)

  • Oh, Son-Mook;Kim, Jung-Han
    • The Journal of the Acoustical Society of Korea
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    • v.39 no.2
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    • pp.120-130
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    • 2020
  • This paper solves the problem in underdetermined convolutive mixture by improving the disadvantages of the multichannel nonnegative matrix factorization technique widely used in blind source separation. In conventional researches based on Spatial Covariance Matrix (SCM), each element composed of values such as power gain of single channel and correlation tends to degrade the quality of the separated sources due to high variance. In this paper, level and frequency normalization is performed to effectively cluster the estimated sources. Therefore, we propose a novel SCM and an effective distance function for cluster pairs. In this paper, the proposed SCM is used for the initialization of the spatial model and used for hierarchical agglomerative clustering in the bottom-up approach. The proposed algorithm was experimented using the 'Signal Separation Evaluation Campaign 2008 development dataset'. As a result, the improvement in most of the performance indicators was confirmed by utilizing the 'Blind Source Separation Eval toolbox', an objective source separation quality verification tool, and especially the performance superiority of the typical SDR of 1 dB to 3.5 dB was verified.

Prostatic Bleeding after Prostatic Biopsy Effects Oncological Outcomes with Laparoscopic Radical Prostatectomy

  • Takai, Tomoaki;Inamoto, Teruo;Komura, Kazumasa;Tsujino, Takuya;Matsunaga, Tomohisa;Yoshikawa, Yuki;Uchimoto, Taizo;Saito, Kenkichi;Tanda, Naoki;Minami, Koichiro;Uehara, Hirofumi;Ibuki, Naokazu;Takahara, Kiyoshi;Nomi, Hayahito;Kiyama, Satoshi;Azuma, Hayahito
    • Asian Pacific Journal of Cancer Prevention
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    • v.17 no.3
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    • pp.1373-1377
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    • 2016
  • Background: We vigorously reviewed patients' operation record who had adhesion of the Denonvilliers' fascia and found out most of these patients had prostatic bleeding after prostatic gland biopsies. We examined the magnitude of prostatic bleeding and frequency after biopsies and the relationship with oncological outcomes. Materials and Methods: A total of 285 patients were selected for the final analyses. Inclusion criteria were as follows: receiving MRI three weeks after biopsiesand laparoscopic radical prostatectomy within 300 days after biopsy. We divided the patients into two groups with (group A) or without (group B) prostatic bleeding. We examined the magnitude of prostatic bleeding after biopsies and the relationship with operation time (OT), positive surgical margin (PSM), biochemical recurrence (BCR) and other factors. Furthermore, we created a logistic-regression model to derive a propensity score for prostatic bleeding after biopsies, which included all patient and hospital characteristics as well as selected interaction terms, and we examined the relationship with PSM and BCR. Results: In all patients, the OT in the group B was shorter than the group A (p < 0.001). Prostatic bleeding was associated with PSM (p=0.000) and BCR (p=0.036). In this propensity-matched cohort, 11 of 116 patients in the group B had PSM as compared with 36 of 116 patients from group A (match-adjusted odds ratio, 4.30; 95%CI confidence interval, 2.06 to 8.96; P=0.000). In addition, eight of 116 patients in group B encountered BCR, as compared with 18 of 116 patients in group A (match-adjusted odds ratio, 2.48; 95%CI, 1.03 to 5.96; P=0.042). Kaplan-Meier analysis in the propensity matching cohort showed a significant biochemical recurrence-free survival advantage for being free of prostate bleeding after biopsies. Conclusions: Our findings in the present cohort should help equip surgeons to pay attention to careful excision especially for those who experienced deferred prostatic bleeding.

Usefulness Evaluation of HRCT using Reconstruction in Chest CT (흉부CT 검사 시 HRCT 영상 재구성의 유용성)

  • Park, Sung-Min;Kim, Keung-Sik;Kang, Seong-Min;Yoo, Beong-Gyu;Lee, Ki-Bae
    • Korean Journal of Digital Imaging in Medicine
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    • v.17 no.1
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    • pp.13-18
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    • 2015
  • Purpose : Skip the repetitive HRCT axial scan in order to reduce the exposure of patients during chest HRCT scan, Helical Scan Data into a reconstructed image, and exposure of the patient change and visually evaluate the usefulness of the HRCT images. Materials and method : Patients were enrolled in the survey are 50 people who underwent chest CT scans of patients who presented to the hospital from January 2015 to March 2015. 50 people surveyed 22 people men and 28 people women people showed an average distribution of 30 to 80 years age was 48 years. 50 patients to Somatom Sensation 64 ch (Siemens) model with 120 kVp tube voltage to a reference mAs tube current to mAs (Care dose, Siemens) as a whole, including the lungs and the chest CT scan was performed. Scan upon each patient CARE dose 4D (Automatic exposure control, Siemens Medical Solution Erlangen, Germany) was to maintain the proper radiation dose scan every cross-section through a device that automatically adjusts the tube current of. CT scan is the rotation time of the Tube slice collimation, slice width 0.6 mm, pitch factor was made under the terms of 1.4. CT scan obtained after the raw data (raw data) to the upper surface of the axial images and coronal images for each slice thickness 1 mm, 5 mm intervals in the high spatial frequency calculation method (hight spatial resolution algorithm, B60 sharp) was the use of the lung window center -500 HU, windows were reconstructed into images in the interval -1000 HU to see. Result : 1. Measure the total value of DLP 50 patients who proceed to chest CT group A (Helical Scan after scan performed with HRCT) and group B (Helical Scan after the HR image reconstruction to the original data) compared with the group divided, analysis As a result of the age, but show little difference for each age group it had a decreased average dose of about 9%. 2. A Radiation read the results of the two Radiologist and a doctor upper lobe and middle lobe of the lung takes effect the visual evaluation is not a big difference between the two images both, depending on the age of the patient, especially if the blood vessels of the lower lobe (A: 3.4, B: 4.6) and bronchi(A: 3.8, B4.7) image shake caused by breathing in anxiety (blurring lead) to the original data (raw data) showed that the reconstructed image is been more useful in diagnostic terms. Conclusion : Scan was confirmed a continuous, rapid motion video to get Helical scan is much lower lobe lung reduction in visual blurring, Helical scan data to not repeat the examination by obtaining HRCT images reorganization reduced the exposure of the patient.

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Performance Analysis of Noncoherent OOK UWB Transceiver for LR-WPAN (저속 WPAN용 비동기 OOK 방식 UWB 송수신기 성능 분석)

  • Ki Myoungoh;Choi Sungsoo;Oh Hui-Myoung;Kim Kwan-Ho
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.30 no.11A
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    • pp.1027-1034
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    • 2005
  • IEEE802.15.4a, which is started to realize the PHY layer including high precision ranging/positioning and low data rate communication functions, requires a simple and low power consumable transceiver architecture. To satisfy this requirements, the simple noncoherent on-off keying (OOK) UWB transceiver with the parallel energy window banks (PEWB) giving high precision signal processing interface is proposed. The flexibility of the proposed system in multipath fading channel environments is acquired with the pulse and bit repetition method. To analyze the bit error rate (BER) performance of this proposed system, a noise model in receiver is derived with commonly used random variable distribution, chi-square. BER of $10^{-5}$ under the line-of-sight (LOS) residential channel is achieved with the integration time of 32 ns and signal to noise ratio (SNR) of 15.3 dB. For the non-line-of-sight (NLOS) outdoor channel, the integration time of 72 ns and SNR of 16.2 dB are needed. The integrated energy to total received energy (IRR) for the best BER performance is about $86\%$.

Monitoring of the Steaming and Drying Conditions for Onion Dehydration (건조양파 제조를 위한 증숙 및 건조 조건 모니터링)

  • Choi, In-Hag;Lee, Gee-Dong
    • Food Science and Preservation
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    • v.19 no.5
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    • pp.703-711
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    • 2012
  • This study was conducted to investigate the quality and organoleptic properties of dehydrated onions with steaming and heating. Sliced onions were treated as follows: steaming time ($X_1$, 5~25 min); drying temperature ($X_2$, $55{\sim}70^{\circ}C$) and drying time ($X_3$, 4~20 hr). All the variables were modelled and analyzed using a central composite design and response surface methodology (RSM). The coefficients ($R^2$) of the water content and water activity model induced from RSM were 0.9514 (p<0.01) and 0.9455 (p<0.01), respectively. The water content and water activity were affected by the drying temperature and time. The $R^2$ on the models of Hunter's L, a, b values were 0.9419 (p<0.05), 0.8818 (p<0.01) and 0.9360 (p<0.01), respectively. Hunter's L, a, b values were affected by the drying temperature and time, but Hunter's b value was not affected by the steaming time. In addition, the $R^2$ on the models of overall palatability was 0.8867 (p<0.05). The maximum palatability response was 5.92 when the steaming time was 13.14 min, the drying temperature was $63.11^{\circ}C$ and the drying time was 14.49 hr.