• Preventive Nutrition and Food Science
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• v.21 no.4
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• pp.310-316
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• 2016

In this study, the anti-osteoarthritis effects of Cynanchum wilfordii, Phlomis umbrosa, and Angelica gigas extract (CPAE), observed and confirmed in previously clinical studies were further investigated by in vitro and in vivo studies. Anabolic biomarkers related to healthy cartilage maintenance, such as aggrecan, type II collagen ${\alpha}$-1 (Col2a1), sex determining region Y-box-9 (Sox-9), and catabolic biomarkers related to osteoarthritis, such as cyclooxygenase-2 (Cox-2), matrix metalloproteinase-13 (Mmp13), and nuclear factor kappa-light-chain-enhancer of activated B cells ($Nf{\kappa}b$), were evaluated by quantitative reverse transcriptase polymerase chain reaction and reporter gene assay. In vitro study results showed significant changes in both anabolic and catabolic biomarkers. For anabolic factors, significant changes in the level of aggrecan (P<0.05), Col2a1 (P<0.05), and Sox-9 (P<0.01) activation were shown after treatment of cartilage cells with CPAE (50 ng/mL) with similar efficacy compared to insulin growth factor, the positive control (100 ng/mL). For catabolic factors, significant changes in the inhibition activity of Cox-2 (P<0.05), Mmp13 (P<0.01), and $Nf{\kappa}b$ (P<0.05) were shown for CPAE (50 ng/mL) with similar efficacy compared to Celecoxib, the positive control ($10{\mu}M$). In the in vivo carrageenan-induced paw edema model study results showed that CPAE-treated groups (100 mg/kg) and Celecoxib-treated groups (60 mg/kg) showed comparably significant efficacy of inhibition by 37.1% and 52.1%, respectively. Furthermore, CPAE (200 mg/kg) showed similar effect to Celecoxib (60 mg/kg) with an inhibition rate of 54.3%. This result confirms that CPAE effectively inhibited the inflammation-induced osteoarthritis symptoms.

• The Korea Journal of Herbology
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• v.30 no.1
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• pp.95-101
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• 2015

Objectives : The aim of this study was to confirm whether or not coral has a preventive effect on development of atopic dermatitis induced by house mite(dermatophagoides pteronyssinus) in NC/Nga mice. Methods : This study was undertaken by using a reliable Atopic dermatitis mouse model demonstrating similar immune response. Lobophytum crassum was administered orally to NC/Nga mouse for 3 weeks. In order to verify the effectiveness of Lobophytum crassum in atopic dermatitis treatment, its role in immune factors were observed in NC/Nga mice. Results : ALT, AST, BUN and creatine levels were all within in the normal ranges in MC-1 200 and 400 (mg/kg) treated groups, indicating no induced toxicity. MC-1 200 and 400 (mg/kg) groups decreased of atopic dermatitis skin manifestation in NC/Nga mouse of MC-1 200 and 400 (mg/kg) groups compared to that of the control group and decreased the ratio of WBC and lymphocyte in blood. Also, MC-1 200 and 400 (mg/kg) groups significant decreased the ratio of CD4+, CD8+, CD11b+/Gr1+, B220/CD23 and CD4/CD25 immune cell ratio in ALN. Finally MC-1 200 and 400 (mg/kg) groups significantly increased the ratio of CD4+, CD8+, B220/CD23 and CD4/CD25 immune cells in DLN. Conclusions : Theses results suggested that Lobophytum crassum has suppressive effects on aberrant and overactive immunological activities in dermatophagoides pteronyssinus-induced dermatitis mice of NC/Nga.

• Maxillofacial Plastic and Reconstructive Surgery
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• v.29 no.6
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• pp.499-508
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• 2007

Background and Purpose: Some subtypes of malignant salivary gland tumors such as adenoid cystic carcinoma (ACC) frequently result in distant metastasis of vascular origin, which are main causes of treatment failure. The reasons for the affinity for vascular metastatic potential are unclear. Therefore, molecular characteristics that influence the dissemination of metastatic tumor cells are important for the design of more effective treatment of salivary ACC. Tumor angiogenesis has been known to be essential for the distant metastasis of malignant cells. So, we determined expressions of vascular metastasis related factors in orthotopic (parotid) murine models of parotid ACC and compared with those in ectopic (subcutis) tumors of athymic mice. Experimental Design: Using specimens from murine parotid (orthotopic, experimental group) and subcutaneous (ectopic, control group) tumors, which have developed via transplantation of tumor cells, originated from human parotid ACC, we performed immunohistochemical assays with anti-vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF, FGF2), matrix metalloproteinase (MMP)-9, and interleukin (IL)-8 antibodies. We also performed immunohistochemical assays with VEGF receptor (VEGFR)-1, VEGFR-2, VEGFR-3, and phosphorylated VEGFR-2. Results: Transplantation of human ACC tumor cell $(5{\times}10^5)$ into the parotid and subcutis successfully resulted in orthotopic (parotid) and ectopic (subcutaneous) tumors in athymic mice. Immunohistochemical staining demonstrated higher expression of major angiogenic factors (VEGF, bFGF, MMP-9) in the orthotopic tumors than in ectopic tumors (P<0.05). But the expression level of angiogenic receptors were same in orthotopic and ectopic tumors of parotid ACC. Conclusion: VEGF, bFGF, and MMP-9 could be a good candidates for antiangiogenic therapy for the contol of vascular metastatic lesions of salivary ACC.

• Ocean Systems Engineering
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• v.10 no.1
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• pp.87-110
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• 2020

Catamaran has recently been a choice to support a typical vertical axis turbine in floating tidal current energy conversion system. However, motion responses associated with the catamaran can reduce the turbines efficiency. The possibility to overcome this problem isto change the catamaran parameter by varying and simulating the demi-hull separations to have lower motion responses. This simulation was undertaken by Computational Fluid Dynamic (CFD) using potential flow analysis. Cases of demi-hull separation were considered, with ratios of demi-hull separation (S) to the breadth of demi-hull (B), ^S/_B of 3.45, 4.95, 6.45, 7.2 and 7.95. In order to compare to the previous works in the literature, the regular wave was set with wave height of 0.8 m. Furthermore, the analysis was carried out by irregular waves with significant wave height, H_s, of about 0.09 to 1.5 m and the wave period, T, of about 1.5 to 6 s or corresponding to the wave frequency, ω, of about 1.1 to 4.2 rad/s. The wave spectrum was derived from the equation of the International Towing Tank Conference (ITTC). For the case of turbines-loaded catamaran under consideration, the new finding is that the least significant amplitude response can be satisfied at the ratio ^S/_B of 7.2. This study indicates that selecting a right choice of demi-hull separation ratio could contribute in reducing motion responses of the tidal current turbines-loaded catamaran.

• International Journal of Advanced Culture Technology
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• v.7 no.4
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• pp.125-136
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• 2019

The radish skin and radish greens are an edible part of the radish. But they are removed before eating the radish and used as a byproduct or an animal feed material because of their tough and rough texture. Melanin is a pigment that gives colour to our skin. But increased production of melanin can turn into benign or malignant tumours. These days due to global warming, the amount of Ultra violet (UVB) rays has been extensively increased with sunlight. Due to this, a phenomenon called exogenous photo aging is widely observed for all skin colour and types. As a result of this phenomenon, a set of enzymes called matrix metalloproteinases (MMP's) that serves as degradation enzymes for extracellular matrix proteins mainly collagen is increased, causing depletion in collagen and resulting in early wrinkles formation. Therefore in our study we used the murine melanoma cell line B16/F10 to study the melanogenesis inhibition by Heated radish extract (HRE) in vitro and we used HRM-2 hair less mice exposed to artificial UVB for checking the efficacy of Heated radish extract in vivo. Furthermore, we prepared a 3% Heated radish extract (HRE) cream and checked its effects on human skin. Our results have clearly demonstrated that Heated radish extract (HRE) have potently suppressed the tyrosinase activity and melanin production in B16/F10 cells. It had also reduced the expression of components involved in melanin production pathway both transcriptionally and transitionally. In in vivo studies, HRE had potently suppressed the expression of MMP's and reduced the wrinkle formation and inhibited collagen degradation. Moreover, on human skin, ginseng cream increased the resilience, skin moisture and enhanced the skin tone. Therefore in light of these findings, we conclude that HRE is an excellent skin whitening and antiaging product.

• The Journal of Korean Academy of Prosthodontics
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• v.27 no.1
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• pp.55-81
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• 1989

Recently the instantaneous center concept has been to understand the biomechanics by which a tissue derangement causes a mechanical derangement in human joint. Therefore, to understand the biomechanics of temporomandibular joint (T.M.J.) as a part of human joint, it is necessary to clarify the instantaneous center of rotation (I.C.R.) in the mandibular movement. Twenty male subjects without T.M.J. disorder and mandibular deviation during the mandibular movement were selected for this study. The habitual opening and closing paths were recorded on the paper of the sagittal metal plate by two pencil markers connected to the resin open clutch attached on the lower teeth, which was designed for this study. The coordinates of the 33-target points and the 109-anatomical landmarks were obtained using a Summagraphic digitizer connected to a 18AT computer. The original raw data of the opening and closing paths were smoothed by B-spline curve fitting technique and then the I.C.R. pathways were determined mathematically by the computer using algorithm for finding the I.C.R. of a planer rigid body model. Also the opening and closing movements of the mandible were simulated according to the determined I.C.R. The results obtained from this study were as follows. 1. At the early opening and the last closing, I.C.R's were almost distributed around the mastoid process outside the mandibular body without the presence in the region of the mandibular condyle. 2. The I.C.R. pathway showed variable patterns to each subject at the opening and closing movements. 3. The K constant with uniform pattern was obtained by the rotation angle times the radius, which was assumed to the index of the mandibular movement. 4. The opening and closing movements of the mandible were simulated by the I.C.R. pathways at the habitual opening and closing movements. 5. The mandibular condyle was rotated or translated accordng to the relative rotation angle and radius of the determinant factors of K contant.

• Restorative Dentistry and Endodontics
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• v.45 no.3
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• pp.32.1-32.12
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• 2020

Objectives: To evaluate the polymerization efficiency of a matrix-modified bulk-fill composite, and compare it to a conventional composite which has a similar filler system. The degree of conversion (DC%) and monomer elution were measured over different storage periods. Additionally, fillers' content was examined. Materials and Methods: Cylindrical specimens were prepared, in bulk and incrementally, from Filtek Bulk Fill (B) and Filtek Supreme XTE (S) composites using a Teflon mold, for each test (n = 6). Using attenuated total reflection method of Fourier transformation infrared spectroscopy, DC% was measured after 24 hours, 7 days, and 30 days. Using high-performance liquid chromatography, elution of hydroxyethyl methacrylate, triethylene glycol dimethacrylate, urethane dimethacrylate, and bisphenol-A glycidyl dimethacrylate was measured after 24 hours, 7 days and 30 days. Filler content was examined by scanning electron microscopy (SEM). Data were analyzed using 2-way mixed-model analysis of variance (α = 0.05). Results: There was no significant difference in DC% over different storage periods between B-bulk and S-incremental. Higher monomer elution was detected significantly from S than B. The elution quantity and rate varied significantly over storage periods and between different monomers. SEM images showed differences in fillers' sizes and agglomeration between both materials. Conclusions: Matrix-modified bulk-fill composites could be packed and cured in bulk with polymerization efficiency similar to conventional composites.

• Proceedings of the Korean Society for Emotion and Sensibility Conference
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• 1999.11a
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• pp.216-221
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• 1999

A mental task combined with noise background is an effective model of laboratory stress for study of psychophysiology of the autonomic nervous system (ANS). The intensity of the background noise significantly affects both a subjective evaluation of experienced stress level during test and the physiological responses associated with mental load in noisy environments. Providing tests of similar difficulties we manipulated the background noise intensity as a main factor influencing a psychophysiological outcome and the analyzed reactivity along withe the noise intensity dimension. The goal of this study was to identify the patterns of ANS responses and the relevant subjective stress scores during performance of word recognition tasks on the background of white noise (WN) of the different intensities (55, 70 and 85 dB). Subjects were 27 college students (19-24 years old). BIOPAC, Grass Neurodata System and AcqKnowlwdge 3.5 software were used to record ECG, PPG, SCL, skin temperature, and respiration. Experimental manipulations were effective in producing subjective and physiological responses usually associated with stress. The results suggested that the following potential autonomic mechanisms might be involved in the mediation of the observed physiological responses: A sympathetic activation with parasympathetic withdrawal during mild 55 and 70dB noise (featured by similar profiles) and simultaneous activation of sympathetic and parasympathetic systems during intense 85dB WN. The parasympathetic activation in this case might be a compensatory effect directed to prevent sympathetic domination and to maintain optimal arousal state for the successful performance on mental stress task. It should be mentioned that obtained results partially support Gellhorn's (1960; 1970) "tuning phenomenon" as a possible mechanism underlying stress response.

• The KIPS Transactions:PartB
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• v.14B no.6
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• pp.413-422
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• 2007

This paper addresses a new technique for constructing surface model from a set of wire-frame contours. The most difficult problem of this technique, called contour triangulation, arises when there are many branches on the surface, and causes lots of ambiguities in surface definition process. In this paper, the branching problem is reduced as the surface reconstruction from a set of virtual belts and virtual canyons. To tile the virtual belts, a divide-and-conquer strategy based tiling technique, called the BPA algorithm, is adopted. The virtual canyons are covered naturally by an iterative convex removal algorithm with addition of a center vertex for each branching surface. Compared with most of the previous works reducing the multiple branching problem into a set of tiling problems between contours, our method can handle the problem more easily by transforming it into more simple topology, the virtual belt and the virtual canyon. Furthermore, the proposed method does not involve any set of complicated criteria, and provides a simple and robust algorithm for surface triangulation. The result shows that our method works well even though there are many complicated branches in the object.

• Proceedings of the Korea Water Resources Association Conference
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• 2012.05a
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• pp.260-264
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• 2012

전 지구적으로 지구온난화로 인해 기후변화가 일어나고 있으며 이에 대해 다양한 방면에서 기후변화에 대한 대응, 적응, 극복을 위한 연구가 활발히 진행되고 있다. 선진국에서는 일찍이 기후 변화관련 영향을 정량적으로 평가하고 치수정책에 반영하고자 노력하고 있으며, 우리나라의 경우도 2000년에 들어서 기후변화 관련 연구를 본격적으로 시작하였다. 지난 100년 동안 한반도 기온은 약 $1.7^{\circ}C$ 상승하여 세계온도의 증가율에 비해 2.3배 상승하였고, 최근 50년 동안 우리나라 강수량을 분석한 결과 전국적으로 강수일수는 감소하고 일강수량 80mm 이상인 호우발생 빈도는 증가하는 것으로 나타났다. 또한, 남부지역에서는 연강수량이 7% 증가하고 연 강수일수는 14% 감소하며 강수강도는 18% 증가하는 것으로 분석되었다. 이상의 결과를 종합해 볼 때, 우리나라는 기후변화의 영향으로 강우일수는 감소하고 연강우량은 증가하는 것을 알 수 있으며, 이는 곧 강우강도가 강해짐에 따라 홍수와 가뭄의 발생가능성이 증가될 것으로 예상된다. 따라서 본 연구에서는 기후변화에 따른 홍수와 가뭄 발생에 대응하기 위한 기초자료를 제공하고자 지석천 유역의 기후변화 시나리오에 따른 유출량 변화를 모의하였으며, 이를 위해 분포형 장기 강우-유출모형인 SWAT(Soil And Water Assessment Tool)모형을 이용하였다. 기후변화 시나리오 자료는 국내 기상청에서 제공하는 수평격자 27km의 고해상도 RCM A1B 시나리오 자료를 사용하였으며, 1971~2010년 기간의 기후변화 시나리오 자료를 지석천 유역에 인접한 광주기상청 실측 기상자료와 비교하여 편이보정 후 2011~2100년 기간의 유출량을 모의하였다. 유출량 모의값에 대한 검 보정을 위하여 지석천유역의 하류지점인 남평지점의 실측 유량을 이용하여 검 보정을 실시하였으며, 2002~2005년 기간의 자료를 이용하였다. 검 보정 결과 2002~205년 기간 동안의 유출량 모의값은 실측유량값과 유사한 경향을 나타내었으며 본 연구의 목표인 2011~2100년까지의 유출량은 기후변화 시나리오의 내용과 비슷한 첨두유량이 증가함을 나타내었다.