• Bulletin of the Korean Chemical Society
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• 제13권3호
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• pp.280-285
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• 1992

Enantiomeric separation of free amino acids has been achieved by a reversed phase liquid chromatography with addition of a Cu(Ⅱ) complex of N-alkyl-L-proline (alkyl: propyl, pentyl or octyl) to the mobile phase. The amino acids eluted were detected by a postcolumn OPA system. N-alkyl-L-proline was prepared and used as a chiral ligand of Cu(Ⅱ) chelate for the enantiomeric separation. The concentration of the Cu(Ⅱ) chelate, the organic modifier and pH affect the enantiomeric separation of free amino acids. The retention behaviour, varied with change in pH and the concentration of the Cu(Ⅱ) chelate, was different compared with those of the derivatized amino acids. The elution orders between D- and L-forms were consistent except histidine showing that L-forms elute earlier than D-forms. The retention mechanism for the enantiomeric separation can be illustrated by the stereospecificity of the ligand exchange reaction and the hydrophobic interaction between the substituent of amino acids and reversed phase, $C_18$.

• 한국통신학회논문지
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• 제21권12호
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• pp.3205-3214
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• 1996

In this paper, the microstrip phased array antennas with coupling-slots for the base station of mobile communication is proposed and anlyzed with accurate analysis method which is based on both reciprocity principle and full-wave analysis. The basis functions used for the numerical analysis are determined depending upon the accuracy, convergence properties of the solution, and the computation time. The patch uses 3 EB mode and the slot uses IPWS mode. The designed phased array antenna has 8 slot-coupled microstrip patch array elements and the beam scanning capability is obtained by using the 4-bit PIN-diode phase shifters as switching devices which are consisted of the loaded line phase shifters for 30.deg. and 60.deg. and the reflection type phase shifters for 90.deg. and 180.deg. repectively. The 4-bits phase shifters which aremade by connecting each phase shifter have about 2.deg.-3.deg. phase errors and their insertion loss are about 3dB for each phase state. The fabricated 8-element phased array antenna with 4-bits phase shifters provides 12.deg.-14.deg. beamwidths depending on the scanning angle and is capable of scanning its beam to .+-.45.deg. with 9.deg. intervals, and the gain 12dBi. The overall results show that the slot-coupled phased array antenna has great advantages of wideband, high gain and reduced spurious radiation. Also, the antenna can be made small and thin. Furthermore, the scanning property of this antenna allows for its application in several areas, such as mobile communication system and PCS.

• 한국전자파학회논문지
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• 제8권6호
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• pp.581-588
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• 1997

시변 페이딩 이동무선채널은 전송 선호의 전폭, 주파수 그리고 위상까지 랜덤하게 왜곡시키며 시간적으로 변하는 스펙트럼 null 을 가진다. 이러한 채널에 대해 위상왜곡에 민감한 성질과 느린 수렴속도를 가지는 기존의 등화기는 열악한 에러 성능을 나타낸다. 이 논문에서는, 단순하면서도 빠른 수렴속도의 Data - Recycling 알고리듬 을 위상왜곡에 강한 Fractionally Spaced 동화기(FSE) 구조에 적용하였으며 시변 이동통신 페이딩 채널에 대 해 다른 기존의 둥화기들과 비교하여 그 성능을 평가하였다. 그 결과 Data-Recycling FSE는 시변 이동통신채널의 특성을 빠르게 추적하는 능력과 위상왜곡에 대한 효과적인 보상능력으로 상당한 BER 성능개선을 나타내었다.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제8권3호
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• pp.857-872
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• 2014

Recent researches reveal that great benefit can be achieved for data gathering in wireless sensor networks (WSNs) by employing mobile data collectors. In order to balance the energy consumption at sensor nodes and prolong the network lifetime, a multi-track large-scale mobile data collection mechanism (MTDCM) is proposed in this paper. MTDCM is composed of two phases: the Energy-balance Phase and the Data Collection Phase. In this mechanism, the energy-balance trajectories, the sleep-wakeup strategy and the data collection algorithm are determined. Theoretical analysis and performance simulations indicate that MTDCM is an energy efficient mechanism. It has prominent features on balancing the energy consumption and prolonging the network lifetime.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제4권3호
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• pp.305-323
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• 2010

This paper proposes an efficient and yet powerful local descriptor called phase-space partition based descriptor (PPD). This descriptor is designed for the mobile image matching and retrieval. PPD, which is inspired from SIFT, also encodes the salient aspects of the image gradient in the neighborhood around an interest point. However, without employing SIFT's smoothed gradient orientation histogram, we apply the region based gradient statistics in phase space to the construction of a feature representation, which allows to reduce much computation requirements. The feature matching experiments demonstrate that PPD achieves favorable performance close to that of SIFT and faster building and matching. We also present results showing that the use of PPD descriptors in a mobile image retrieval application results in a comparable performance to SIFT.

• Journal of Pharmaceutical Investigation
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• 제21권1호
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• pp.33-41
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• 1991

A high-performance liquid chromatographic (HPLC) method was developed for the simultaneous determination of theophylline(TP) and its metabolites, 1-methyluric acid (1-MU) and 1,3-dimethyluric acid (1,3-DMU), in rat plasma and urine. An $100\;{\mu}l$ aliquot of a plasma or urine sample was mixed with $250\;{\mu}l$ of acetonitrite and vortexed. After centrifugation, $200\;{\mu}l$ (plasma) or $20\;{\mu}l$ (urine) aliquot of the supernatant was dried by $N_2$ stream and redissolved in $100\;{\mu}l$ (plasma) or $200\;{\mu}l$ (urine) of the mobile phase. A $20\;{\mu}l$ of the mobile phase solution was injected onto a $C_{18}$ reversed-phase column. The column was maintained at $45^{\circ}C$ by the aid of electric heating jacket. The mobile phase was a 3%(v/v) methanol solution in deionized water which contains sodium acetate (100 mM) and tetrabutyl ammonium hydroxide (4 mM). pH of the mobile phase was adjusted 4.5 by the addition of acetic acid. Detection limits for TP, 1-MU, and 1,3-DMU in plasma were 0.2, 0.1 and $0.1\;{\mu}/ml$, respectively and the corresponding values in urine were all $5\;{\mu}g/ml$. Inter- and intra-day variability of the assay for all compounds in the plasma samples was less than 5.5 and 3.8%, respectively. The retention times for 1-MU, 1,3-DMU, and TP were approximately 7, 8.5 and 18 min, respectively. Sample preparation procedure used in this method was simple, rapid and reproducible. Renal clearance of TP and its metabolites in rats showed plasma concentration dependency indicating renal tubular secretion and reabsorption of them.

• 공업화학
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• 제8권2호
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• pp.286-291
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• 1997

항암제로 탁월한 효능이 있는 taxol을 정상 액체 크로마토그래피를 이용하여 주목나무의 추출물에서 분리하였다. Taxol을 분리하기 위해 시료 주입량은 $5{\mu}l$에서 $100{\mu}l$로 변화시키고, 이동상 유속은 1ml/min로 고정하여 일정 용매법으로 실험을 하였다. 실험에 사용된 이동상은 헥산, 에탄올, 메탄올, 1-프로판올, 이소프로판올이었다. Taxol과 유사한 성질를 갖는 표준시료 cephalomannine, taxol, 10-deacetyltaxol의 혼합물에서 taxol을 분리하였다. Cephalomannine, taxol, 10-deacetyltaxol 혼합물의 분리 실험 결과로 taxol의 분리 최적 조건은 2성분계에서 헥산/에탄올의 부피 조성비가 96/4이며, 3성분계에서는 헥산/1-프로판올/메탄올의 부피 조성비가 96/2/2이었다. 분리시간은 2성분계에서 80분인데 비해 3성분계에서는 50분으로 적은 용매를 사용하여 분리할 수 있었다. 위에서 얻는 실험조건으로 실제 주목나무의 추출물 $1{\mu}g$에서 taxol를 분리할 수 있었다.

• Bulletin of the Korean Chemical Society
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• 제26권8호
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• pp.1246-1250
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• 2005

We have evaluated the hydroxyl group-solvent specific interactions by using a Lichrosorb RP18 stationary phase and by measuring the retention data of carefully selected solutes in 50/50, 60/40, 70/30, 80/20, and 90/10(v/v%) methanol/water eluents at 25, 30, 35, 40, 45, and 50 ${^{\circ}C}$. The selected solutes are 3 positional isomers of phenylpropanol, that is, 1-phenyl-1-propanol, 1-phenyl-2-propanol, and 3-phenyl-1-propanol. There exist clear discrepancies in ${\Delta}H^o$ (solute transfer enthalpy from the mobile to the stationary phase) and $T{\Delta}S^o$ (solute transfer entropy) among positional isomers. The difference in ${\Delta}H^o$ and $T{\Delta}S^o$ between secondary alcohols (1-phenyl-1-propanol and 1-phenyl-2-propanol)is negligible compared to the difference between the primary alcohol (1-phenyl-3-propanol) and secondary alcohols. The $T{\Delta}S^o$ values of 3-phenyl-1-propanol are close to those of butylbenzene while the $T{\Delta}S^o$ values of secondary alcohols are close to those of propylbenzene. The difference in ${\Delta}{\Delta}H^o$ (specific solute-mobile phase interaction enthalpy) between the primary alcohol and the secondary alcohol decreases with increase of methanol content in the mobile phase. A unique observation is an extremum for 1-phenyl-3-propanol in the plot of $T{\Delta}{\Delta}S^o$ vs. methanol volume %. The positive sign of $T{\Delta}{\Delta}S^o$ of 3-phenyl-1-propanol implies that the entropy of 3-phenyl-1-propanol is greater than that of the hypothetical alkylbenzene (the same size and shape as phenylpropanol) in the mobile phase.

• Bulletin of the Korean Chemical Society
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• 제32권10호
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• pp.3597-3602
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• 2011

A simple and rapid analytical method was developed for the determination of eight isoquinoline alkaloids in Corydalis species. Eight isoquinoline alkaloids, including 2 aporphine alkaloids (isocorydine and glaucine) and 6 protoberberine alkaloids (coptisine, palmatine, berberine, canadine, corydaline, and tetrahydrocoptisine) were used as chemical markers which have a various biological activity and determined for quality control of Corydalis (C.) species (C. ternata, C. yanhusuo, and C. decumbens). To evaluate the quality of these herbal medicines, LC chromatographic separation of alkaloids were preferentially investigated on reversed-phase C18 column with pH variation and composition of mobile phase. In addition, the separation of these alkaloids in herbal extracts was found to be significantly affected on mobile phase composition using gradient elution. Especially for C. yanhusuo extract, berberine was seriously interfered with other alkaloid extracted from sample matrix when mobile phase composition was not optimized. As results, these compounds were successfully separated within 28 min using 10 mM ammonium acetate containing 0.2% triethylamine (adjusted at pH 5.0) as a mobile phase with gradient elution. On the basis of optimized HPLC conditions, 23 different Corydalis species samples were analyzed for the determination of alkaloid levels. In addition, principal component analysis (PCA) combined with the chromatographic data could be successfully classified the different geographic origin samples.

• KSBB Journal
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• 제15권6호
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• pp.605-608
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• 2000

Oligodeoxynucleotides (ODNs) were separated by high-performance membrane chromatography (HPMC), a combined system of chromatography and membrane. The separation mechanism involved anion-exchange, and the stationary phase was cation CIM (Convective Interaction Media) DEAE disk (16${\times}$3 mm). Two types of mobile phase were used, buffer A (20mM Tris-HCl, pH 7.4) and buffer B (buffer A + 1M NaCl). As the amount of NaCl dissolved in buffer linearly increased, the retention time shortened, which enabled a gradient elution mode. Based on the number of theoretical plates and resolution observed, the optimum mobile phase and operating condition (Buffer A/Buffer B=50/50 - 20/80 vol%, gradient time 2 min) were experimentally determined. In this experimental condition, ODNs were separated within 2 min at a mobile phase flow rate of 6 ml/min.