• Title/Summary/Keyword: mixed-culture

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Butyric Acid Fermentation of Sodium Hydroxide Pretreated Rice Straw with Undefined Mixed Culture

  • Ai, Binling;Li, Jianzheng;Chi, Xue;Meng, Jia;Liu, Chong;Shi, En
    • Journal of Microbiology and Biotechnology
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    • v.24 no.5
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    • pp.629-638
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    • 2014
  • This study describes an alternative mixed culture fermentation technology to anaerobically convert lignocellulosic biomass into butyric acid, a valuable product with wide application, without supplementary cellulolytic enzymes. Rice straw was soaked in 1% NaOH solution to increase digestibility. Among the tested pretreatment conditions, soaking rice straw at $50^{\circ}C$ for 72 h removed ~66% of the lignin, but retained ~84% of the cellulose and ~71% of the hemicellulose. By using an undefined cellulose-degrading butyrate-producing microbial community as butyric acid producer in batch fermentation, about 6 g/l of butyric acid was produced from the pretreated rice straw, which accounted for ~76% of the total volatile fatty acids. In the repeated-batch operation, the butyric acid production declined batch by batch, which was most possibly caused by the shift of microbial community structure monitored by denaturing gradient gel electrophoresis. In this study, batch operation was observed to be more suitable for butyric acid production.

Effect of feeding mixed microbial culture fortified with trace minerals on ruminal fermentation, nutrient digestibility, nitrogen and trace mineral balance in Sheep

  • Kwak, W.S.;Kim, Y.I.;Choi, D.Y.;Lee, Y.H.
    • Journal of Animal Science and Technology
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    • v.58 no.5
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    • pp.21.1-21.8
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    • 2016
  • Background: The aim of the present study was to determine the effects of feeding trace mineralsfortified mixed microbial culture (TMC) on ruminal fermentation, nutrient digestibility, blood electrolyte status, nitrogen balance, and trace mineral balance in sheep. Methods: Mixed microbes [0.6 % (v/w) of Enterobacter sp., Bacillus sp., Lactobacillus sp., and Saccharomyces sp.] were cultured with 99 % feedstuffs and 0.4 % trace minerals including zinc and copper for ensiling. Six sheep (a mean body weight of $46.5{\pm}1.2kg$) were fed two diets: a control diet (concentrate mix and rye straw) and an experimental diet (a control diet + 3.1 % TMC). Results: TMC feeding did not induce negative effects on ruminal fermentation, nutrient digestibility, blood electrolytes, and nitrogen balance in sheep. Feeding with TMC increased the intake of trace minerals (p < 0.05) and did not affect absorption of trace minerals in the whole digestive tract. Feeding with TMC increased fecal excretion and absorbable intake, and retention of zinc and copper (p < 0.05) by 71 % and 77 %, respectively. Conclusion: Feeding with TMC resulted in higher zinc and copper bioavailability and retention without any adverse effects on sheep performance.

Interaction between Lactobacillus acidophilus and Saccharomyces uvarum on Utilization of Galacto-oligosaccharides in Soymilk (Lactobacillus acidophilus와 Saccharomyces uvarum의 혼합배양에 의한 두유의 발효 중 당이용에 미치는 작용)

  • 유주현;진효상;류인덕
    • Microbiology and Biotechnology Letters
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    • v.17 no.6
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    • pp.533-538
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    • 1989
  • The enhanced growth and lactic fermentation of L. acidophilus, when mixed with S. uvarum, was investigated. Spent medium of S. uvarum, at 10%, stimulated the growth and lactic fermentation of L. acidophilus, and also increased the content of monosaccharide while decreased the contents of sucrose, raffinose, and stachyose in soymilk. While single culture of L. acidophilus consumed only the monosaccharides in soymilk, the mixed culture of L. acidophilus and S. uvarum consumed almost all the oligosaccharides as well as the monosaccharides in soymilk. Thus it was assumed that S. uvarum converted the oligosaccharides into monosaccharides so that L. acidophilus can produce more lactic acid and cell mass by using the increased monosaccharides.

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Isolating Microorganisms to Ferment Traditional Cheongtaejeon (발효차 청태전 제조용 미생물의 분리)

  • Park, Jung-Suk;Cho, Jung-Il
    • Journal of the Korean Society of Food Culture
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    • v.26 no.2
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    • pp.190-197
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    • 2011
  • Chungtaejeon is a traditional tea introduced in the age of the Three States and is the only "Don-cha" culture in the world that survived on the southwestern shore of Korea. To restore Chungtaejeon and to make the tea with consistent quality, the microorganisms involved in traditional type fermentation of Chungtaejeon were isolated, and the tea was prepared with high fermentation ability starters. The sensuous characteristics of Chungtaejeon were also examined. Only Bacilli were found in 3 and 5 year aged Chungtaejeon samples. The Lactobacilli were isolated from properly fermented kimchi and one of them showed high growth capability in media containing green tea extract and also showed strong antagonistic activity against methicillin-resistant Staphylococcus aureus, S. aureus, Salmonella, and E. coli. It was identified and named Lactobacillus plantarum CHO25. Chungtaejeon was fermented with a single starter of L. plantarum CHO25 and with a mixed starter (L. plantarum CHO25, Saccharomyces cerevisiae and Bacillus amyloliquefaciens CHO104). The single fermented sample had the highest cell growth after 5 days of inoculation and the level decreased slowly thereafter. The mixed fermented sample showed strong growth of S. cerevisiae. The highest hunter values were the a value of the single fermented sample and the b value of the mixed sample. The single fermented tea showed the best incense score.

Studies on the mixed culture of Lactobacillus helveticus and Streptococcus thermophilus in milk - Growth characteristics and cultural conditions for the mixed culture - (Lactobacillus helveticus와 Streptococcus thermophilus의 혼합배양에 관한 연구 -생육특성 및 배양조건-)

  • 윤성식;박정길;유주현
    • Microbiology and Biotechnology Letters
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    • v.13 no.2
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    • pp.151-156
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    • 1985
  • To investigate the new Yoghurt starter, Lactobacillus helveticus YM-1, which was selected among 14 Lactobacillus strains, and Streptococcus thermophilus CH-1 were inoculated together in reconstituted non-fat skim milk medium and their growth characteristics and cultural conditions for the mixed culture were examined. The main results of this study were obtained as follows. The typical symbiotic growth was shown between the two strains and pH and temperature for optimal growth were 6.5 and 4$0^{\circ}C$, respectively. Heat treatment of milk was most effective at 10$0^{\circ}C$ for 30 min. The cell-free filtrate of Lactobacillus helveticus YM-1 had stimulatory effect on Streptococcus thermophilus CH-1 but the reverse case was slightly observed. Significant difference was observed in the proteolytic activities between Lactobacillus helveticus YM-1 and Streptococcus thermophilus CH-1. The former liberated 135$\mu\textrm{g}$ free amino acid per $m\ell$ of cultured milk, the latter 35$\mu\textrm{g}$ per $m\ell$.

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Plant Regeneration and Bulblet Formation of Allium wakegi Araki

  • Song, Won-seob;Yang, Deok-Chun;Yoon, Jae-Ho;Ryu, Sang-Hyun
    • Plant Resources
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    • v.7 no.1
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    • pp.7-14
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    • 2004
  • Allium wakegi was cultured shoot tip in the condition of light culture. The Allium wakegi added plant growth regulator was observed of plant regeneration and bulblet formation. Callus Induction and growing rate was the best of 78% when added alone 2,4-D 0.5mg/L. In the formation of shoot, its regeneration rate was 96% when added BA 0.5mg/L in the light culture condition. When BA 0.5mg/L and NAA 0.5mg/L mixed and BA 0.5 mg/L and NAA 1.0mg/L mixed, the rates were 99% and 97% respectively, and these conditions were suitable for forming shoot. In the formation of roots, when added NAA 2.0mg/L in the light culture condition, the regeneration rate was 90.6 % and the roots were abnormal. When added NAA 1.0mg/L, the rate was 82 % and the highest. In the formation of bulbs, when BA 05mg/L and NAA 1.0mg/L mixed, the root generantion and its size in the bulbs was the best compare to other treatment experiments.

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The Method Research for Analyzing Contemporary Fashion Phenomena - Focused on Mass Culture Theory -

  • Kim, Seo-Youn;Park, Kil-Soon
    • The International Journal of Costume Culture
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    • v.4 no.3
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    • pp.195-202
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    • 2001
  • Re purpose of this study is to indicate the framework to analyze the contemporary fashion phenomena taking on aspect as above by grafting the mass culture theory onto the study for fashion phenomena. The contemporary culture phenomena look like a same thing apparently, but various individual characters appeared in them. And all cultures are mixed in the name of mass culture, but the subordinate concepts which can classify the culture are still in existence. And this equally appear in the contemporary fashion, one of culture phenomena. The concept of the contemporary mass culture can be explained with the taste culture, Cans' theory indicated, divided into highbrow culture, middlebrow culture, and lowbrow culture. And the phenomena of the contemporary mass culture also can be explained with globalization that came down to homogenization, regionalization hybridization.

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Research of Baekje Culture-Products about Chungnam Area National Museum (충청지역 국립박물관을 중심으로 한 백제 문화상품 현황조사)

  • Lee, Sang-Mi;Kim, Chung-Ho
    • Journal of the Korea Furniture Society
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    • v.18 no.3
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    • pp.224-233
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    • 2007
  • What is culture-products? Culture-products contains identity of nation and region. French critic Guy Sorman says "It is natural that buying culture-products when you travel any countries" Culture-products which contains our own cultural factors will give positive image of country. The research and development of grafting grudge experience culture-products was advanced the characteristic of area-culture that necessary is prerequisite. We need to develop culture-products which mixed regional culture and characteristic. We would like to research about Baekje culture and culture-products and sales products in museum. Museum is one of the tourist's attractions and great place to express regional factors. The culture-products are specially limited in the products which reflects a Baekje culture with Muryeong's tomb in Gong-ju and Baekje incense burner in Bu-Yeo it advanced. We hope that this research could help to understand market of culture-products and development.

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Poliovirus Decrease Effect by Activated Sludge Microbes (활성슬러지 구성 미생물에 의한 폴리오바이러스의 감소 효과)

  • Kim, Tae-Dong;Choi, Dong-Hyuk
    • Journal of Environmental Health Sciences
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    • v.32 no.4 s.91
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    • pp.336-341
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    • 2006
  • The biological wastewater treatment system is known to have an important role in reducing the quantify of enteric virus in water environments. To clarify the roles of activated sludge microbes in decreasing the virus infectivity, the behavior of the virus in bacteria, protozoa, and metazoa was examined by pure or mixed culture system using poliovirus type 1(Lsc, 2ab strain). In the bacterial culture systems, the virus infectivity in the liquid phase decreased by a reversible adsorption of the virus to the bacteria or bacterial flocs. On the other hand, in the protozoa and the metazoa culture systems using T. pyriformis and P. erythrophthalma, respectively, with a variety of bacterial strains as prey, the main virus decrease mechanism of reversible adsorption in early stage was changed to irreversible predation, which was not eluted in this study. The virus decrease was more effective in the P. erythrophthalma culture system, which had high predation and floc forming abilities. However, in the mixed culture system of Z. ramigera and P. erythrophthalma, the more rapid reversible adsorption of virus to Z. ramigera flocs preceded the irreversible predation of P. erythrophthalma.

A Study on Intraculturalism in 21st Century Fashion (21세기 패션에 나타난 인트라컬추럴리즘(Intraculturalism)에 관한 연구)

  • Yang, Sook-Hi;Chung, Se-Hui
    • Journal of the Korean Society of Costume
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    • v.59 no.1
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    • pp.119-135
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    • 2009
  • The purpose of this study was to examine formative aesthetic characteristics and aesthetical value of the intraculturalism expressed in contemporary fashion and to confirm the functions of intraculturalism to establish, visualize, perform the racially indeterminate, ethnically neutral, culturally diverse or ambiguous identity. For this study, the applications of the intraculturalism shown in mass media and consumer culture, such as music, fashion advertisements and collections of high fashion designers from 2004 to 2008 have been analyzed and compared. The results were as follows: The Intraculturalism is reflected in the muticultural music such as Afropean, Jawaiian, Reggaeton and Asian Hip Hop. Intracultural music genres create the hybrid music and fashion culture through mixing, matching and blending one and another culture. Advertisement campaigns for Louis Vuitton, YSL Beauty, Gap and H&M stores have all purposely highlighted models with mixed racial heritage. It is reflected in the latest youth fashion market trend using face that are ethnically ambiguous. The increasingly multiracial, multicultural population is due to intermarriage and waves of immigration. The rising mixed race designers, Narciso Rodriguez, Hussein Chalayan, Vera Wang and DooRi Chung, not only compromise and amalgamate different cultural elements of their heritage and contemporary life but also create new look and fashion image. The characteristics of intraculturalism expressed in the 21st century fashion could categorized into de-genre, de-nationality, de-race and de-culture.