• 제목/요약/키워드: mixed-culture

검색결과 1,225건 처리시간 0.031초

Mixed Methods Research on the Intercultural Sensitivity of Summer Program Participants; Comparison of US Participants and Korean Language Partners

  • Soyoung Jeong
    • International Journal of Advanced Culture Technology
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    • 제11권4호
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    • pp.112-122
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    • 2023
  • This study was conducted as part of a summer Korean language and culture program at a university in Korea, aimed at examining the changes in intercultural sensitivity among American students who participated in the program, as well as the Korean students who served as their language partners. The research utilized a mixed-method approach combining qualitative and quantitative analysis. To derive research findings, pre- and post-program Intercultural Sensitivity Scale surveys were administered to participating students and semi-structured interview data were collected from a randomly selected group of six students. The research findings indicated that among a total of twenty-seven students including both American and Korean students, significant changes in intercultural sensitivity were observed in the domain of Interaction Engagement. Furthermore, fourteen American students showed significant changes in their Interaction Confidence, while the Korean students who participated as language partners did not exhibit significant changes in the five subdomains of intercultural sensitivity.

Lactobacillus acidophilus와 Saccharomyces uvarum의 혼합배양에 의한 대두유의 당 변화 (Changes of Oligosaccharide in Soymilk with Mixed Cultures of Lactobacillus acidophilus and Saccharomyces uvarum)

  • 공인수;공재열;유주현
    • 한국식품과학회지
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    • 제24권6호
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    • pp.586-591
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    • 1992
  • 대두유의 oligosaccharide를 분해할 수 있는 S. uvarum을 이용하여 L. acidophilus와 혼합배양을 행하여 당대사의 상호작용을 검토한 결과 대두유에 glucose, fructose, galactose 등의 단당류를 첨가하여 L. acidophilus 단독배양만으로도 높은 산생성을 보여주었으며 혼합배양의 경우도 단당류를 잘 이용하였다. Sucrose가 첨가된 배지에서의 L. acidophilus의 단독배양에서는 산생성이 낮았으나 혼합배양을 했을 때는 다른 단당류만큼 증가되었다. 배양시간에 다른 soywhey의 당변화를 검토한 결과 L. acidophilus 배양에서는 당변화가 거의 없었으나 S. uvarum은 sucrose, stachyose, raffinose와 같은 oligosaccharide를 분해하고 있어 혼합배양중 oligosaccharide로부터 분해된 단당류를 L. acidophilus가 젖산 발효에 이용하는 것으로 생각되었다.

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Degradation of Phenanthrene by Bacterial Strains Isolated from Soil in Oil Refinery Fields in Korea

  • KIM JEONG DONG;SHIM SU HYEUN;LEE CHOUL GYUN
    • Journal of Microbiology and Biotechnology
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    • 제15권2호
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    • pp.337-345
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    • 2005
  • The degradation of phenanthrene, a model PAH compound, by microorganisms either in the mixed culture or individual strain, isolated from oil-contaminated soil in oil refmery vicinity sites, was examined. The effects of pH, temperature, initial concentration of phenanthrene, and the addition of carbon sources on biodegradation potential were also investigated. Results showed that soil samples collected from four oil refinery sites in Korea had different degrees of PAH contamination and different indigenous phenanthrene-degrading microorganisms. The optimal conditions for phenanthrene biodegradation were determined to be 30$^{circ}C$ and pH 7.0. A significantly positive relationship was observed between the microbial growth and the rate of phenanthrene degradation. However, the phenanthrene biodegradation capability of the mixed culture was not related to the degree of PAH contamination in soil. In low phenanthrene concentration, the growth and biodegradation rates of the mixed cultures did not increase over those of the individual strain, especially IC10. High concentration of phenanthrene inhibited the growth of microbial strains and biodegradation of phenanthrene, but was less inhibitory on the mixed culture. Finally, when non-ionic surfactants such as Brij 30 and Brij 35 were present at the level above critical micelle concentrations (CMCs), phenanthrene degradation was completely inhibited and delayed by the addition of Triton X100 and Triton N101.

Evaluation of Mixed Probiotic Starter Cultures Isolated from Kimchi on Physicochemical and Functional Properties, and Volatile Compounds of Fermented Hams

  • Kim, Young Joo;Park, Sung Yong;Lee, Hong Chul;Yoo, Seung Seok;Oh, Sejong;Kim, Kwang Hyun;Chin, Koo Bok
    • 한국축산식품학회지
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    • 제36권1호
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    • pp.122-130
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    • 2016
  • The objective of this study was to investigate the effects of mixed starter cultures isolated from kimchi on physicochemical properties, functionality and flavors of fermented ham. Physicochemical properties, microbial counts, shear force, cholesterol contents and volatile compounds of fermented ham were investigated during processing (curing and ripening time). Curing process for 7 d increased saltiness, however, decreased hunter color values (L, a, and b values). Ripening process for 21 d increased most parameters, such as saltiness, color values, weight loss, shear force and cholesterol content due to the drying process. The mixed starter culture had higher lactic acid bacteria than the commercial one. While eight volatile compounds were identified from fermented hams during curing process, total fiftyeight volatile compounds were identified from fermented hams during ripening process. The main volatile compounds were alcohols, esters and furans. However, no differences in volatile compounds were observed between two batches. Fermented hams (batch B) manufactured with probiotic starter culture (LPP) had higher sensory score in texture, color and overall acceptability than counterparts (batch A), while the opposite trend was observed in flavor. Therefore, mixed probiotic starter culture isolated from kimchi might be used as a starter culture to be able to replace with commercial starter culture (LK-30 plus) for the manufacture of fermented ham.

Lactobacillus bulgaricus 와 Kluyveromyces fragilis의 혼합배양에 의한 두유의 젖산발효 (Lactic Acid Fermentation of Soymilk by Mixed Cultures of Lactobacillus bulgaricus and Kluyveromyces fragilis)

  • 유주현;류인덕;박정길;공인수
    • 한국식품과학회지
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    • 제19권3호
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    • pp.263-272
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    • 1987
  • Lactobacillus bulgaricus KFCC 35463과 Kluyveromyces fragilis KFCC 35458 을 두유 배지에 접종하여 생육과 산생성의 특성을 조사한 결과 두유를 L. bulgaricus로 단독발효하는 것보다 K. fragilis와 혼합발효하는 것이 산생성량이 많고 산생성속도가 빨랐다. 이 두 균을 혼합배양하여 산생성을 높이기 위한 배양조건을 검토한 결과는 다음과 같았다. 두유에 sucrose를 1.0% 또는 탈지분유를 1.5% 첨가하고 L. bulgaricus와 K. fragilis 를 $O.\;D._{660}$ 값으로 1 : 2가 되게 접종하여 $35{\sim}37^{\circ}C$의 온도에서 24시간 배양하는 것이 효과적이었다. L. bulgaricus를 단독 또는 K. fragilis와 혼합배양할 때의 산도는 각각 0.14%와 0.41%로서 혼합배양한 것이 2.9배 높았다. sucrose를 1.0% 첨가한 두유를 사용하였을 경우는 각각 0.13%와 0.70%로서 혼합배양한 것이 5.4배 높았다. 그리고 두유에 탈지분유를 1.5% 첨가하였을 경우는 단독해양한 것과 혼합배양한 것 모두가 0.84% 수준이었고 탈지분유를 1.5% 이하로 첨가하였을 때만 단독배양한 것보다 흔합배양한 것이 산도가 높았다.

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Bacillus natto와 B. licheniformis 혼합 Starter로 제초된 청국장의 품질특성 (Quality Characteristics of the Chungkookjang Fermented by the Mixed Culture of Bacillus natto and B. licheniformis)

  • 연규춘;김동호;김정옥;육홍선;조재민;변명우
    • 한국식품영양과학회지
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    • 제31권2호
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    • pp.204-210
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    • 2002
  • 청국장의 산업적인 대량생산 system에 B. subtilis, B. natto, B. licheniformis 그리고 B. natto와 B. licheniformis의 혼합 균주를 starter로 하여 45시간 동안 청국장을 발효시키면서 각 시험제품의 미생물 생장, 일반성분, 효소활성, 향기성분 등을 분석하고 관능을 평가하였다. 청국장의 수분은 점질물의 형성이 많은 B.subtilis와 B. natto 시험구에서 다소높았으며 pH는 B. licheniformis에서 8.68로 가장 높았고 B. subtilis 에서 8.13으로 가장 낮았다. 미생물의 생장정도는 B. subtilis가 $10^{9}$ CFU/g까지, B. licheniformis는 $10^{8}$ CFU/g까지 생장하였으며 단백질분해효소의 활성은 B. subtilis에서 1.35 unit/g으로 가장 높았고 B. licheniformis에서 0.45 unit/g으로 가장 낮았다. 암모니아태 질소의 함량 은미생물의 생장률 및 단백질분해 효소의 활성에 비례하여 B. subtilis > B. natto> B. licheniformis의 순으로 높은 함량을 나타내었다. 한편, B. natto와 B. licheniformis의 혼합균주를 starter로 하여 제조한 청국장의 일반 품질은 B. natto와 B. licheniformis를 단독 접종한 제품의 평균치에 해당하는 특성을 보였다. 검출된 향기성분의 화학적 구성은 ketones 9종, alcohols 11종, aldehyde 6종, heterocyclic compounds 4종, ester 6종, acids 4종, hydrocarbons 4종, 기타 14종이었으며 균주별로는 B. subtilis에서 25종, B. natto에서 30종, B. licheniformis에서 28종, B. natto와 B. licheniformis를 혼합하여 접종한 청국장에서는 41종의 향기성분이 검출되었다. 유기산의 총량은 B. subtilis를 접종한 청국장에서 3.98%로 가장 높았고 B. natto는 2.24%, B. licheniformis는 1.53%이었으며 B. natto와 B. licheniformis의 혼합 시험구에서 는 1.67%의 함량을 보였다. 분석된 향기성분과 유기산은 starter로 접종된 Bacillus의 종류에 따라 각각 특징적 인 조성을 나타내었다. 관능평가 결과 색, 향, 맛 그리고 전체적인 선호도의 모든 항목에서 B. natto와 B. licheniformis의 혼합 시료가 가장 높은 선호도를 보였고 B. subtilis를 starter로 한 시료의 선호도가 가장 낮았다.

Lipomyces starkeyi와 Leuconostoc mesenteroides의 혼합배양에 의한 올리고당과 Dextran의 생성 및 생성당의 특성 연구 (Development of a Mixed-culture Fermentation Process and Characterization for New Oligosaccharides and Dextran Using Lipomyces starkeyi and Leuconostoc mesenteroides)

  • 허수진;김도만;이인수;장판식
    • 한국미생물·생명공학회지
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    • 제27권4호
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    • pp.304-310
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    • 1999
  • We have developed a new process for the production of new structure oligosaccharides using the mixed-culture fementation of Lipomyces starkeyi KSM22 and leuconostoc mesenteroides B-512FMCM.L.starkeyi KSM22 produces a novel DXAMase(an enzyme containing both dextranase and amylase activities). It hydrolyzes the soluble starch and dextran. The hydrolyzates were used as acceptors for dextransucrase of L.mesenteroides to synthesize the new oligosaccharides(NOS). In fermentation, as the concentration of sucrose was increased from 9%(w/v) to 15%(w/v), the yields of dextran(sum of dextran I, MW=66kD, and dextran II, MW=21kD) was increased from 12.7% to 42.5%, and NOS was increased from 3.9% to 5.2% of the theoretical, respectively. The NOS of dp(degree of polymerization) 5 and over was increased from 33.1% to 58.3% of the total NOS. The NOS showed heat resistant up to 12$0^{\circ}C$ and was stable at pHs ranged from 2 to 6. The NOS decreased the pH changes in the culture of S. mutans, and also showed inhibitory effects on the growth of S. aureus or S. typhimurium.

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반연속배양의 혼합균주에 의한 Benzene, Toluene 및 Phenol 혼합물 분해 (Biodegradation of Benzne,Toluene, and Phenol by a Mixed Culture in Semicontinuous Culture)

  • 오희목;김성빈;이창호;서현효;이문호;고영희;윤병대
    • 한국미생물·생명공학회지
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    • 제22권4호
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    • pp.415-422
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    • 1994
  • The biodegradation of aromatic compounds by a mixed culture GE1 was investigated in an artificial wastewater containing 250 mg/l of benzene, toluene, and phenol in semicontinuous culture. In the control group (no strains) with an aeration rate of 75 ml/l/min, 37% of phenol and 83% of benzene were volatilized during early 24 hrs and toluene was disappeared from the medium within 12 hrs. The biodegradation of benzene and toluene was effective in SB (strains + biofilm) treatment, while phenol was degraded more quickly in SG (strains + glucose) treatment including glucose as an additional carbon source. aromatic compounds added at a concentration of 250 mg/l were completely removed by SG treatment after 16 hrs or 32 hrs, respectively. The removal rate of COD was high as much as 80 mg/l/h in SG treatment during early period, but COD revealed a stable value of 116~140 mg/l after 12 hrs caused by increased biomass. Therefore, it is concluded that the mixed GE1 could be used for the wastewater treatment including aromatic compounds such as benzene, toluene, and phenol.

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다문화가정 부부의 가정생활 적응 요인에 대한 연구 (Adjustment Factors for Family Life of Multi Cultural Family Couples)

  • 강기정;정천석
    • 가족자원경영과 정책
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    • 제13권2호
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    • pp.153-167
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    • 2009
  • This research analyzes factors of family life adjustment in mixed-culture families through a focus group and individual interviews with nine mixed-culture couples eighteen people who live in city C. First, the reason that women of other cultures wants to marry Korean men is because they find it a good opportunity, as they expect that Korean husbands will take responsibility of their family. On the other hand, Korean men also expect that they would have some similarities to each other. Also, in southeastern Asian men have more freedom than women to choose an international marriage in Southeastern Asia. Second, mixed-culture couples in everyday life are affected by communication problems, cultural differences, natural environment, financial situation, male -dominant attitude, self-adjustment pressure, house-work role and financial support from their own country. Third, wives from other cultures ask for social support to adjust in Korea and make a healthy family. These women can be important, as they have their own role in the family even though they may be far from their mother in law's strong expectation in how well she can adopt Korean culture.

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유산균이 Aspergillus parasiticus의 성장과 Aflatoxin $B_1$ 생성에 미치는 영향 (The Effects of Lactic Acid Bacteria on the Growth and Aflatoxin $B_1$ Production of Aspergillus parasiticus)

  • 이경민;김종규
    • 한국환경보건학회지
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    • 제31권2호
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    • pp.127-133
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    • 2005
  • This study was performed to investigate the competitive effect of Leuconostoc mesenteroides and Lactobacillus plantarum on the growth and aflatoxin $B_1$ production of Aspergillus parasiticus. Leu. mesenteroides and Lac. plantarum were grown with A. parasiticus in a modified APT broth at $28^{\circ}C$ for 9 days. It was observed that the growth of A. parasiticus got lower when the two bacteria were inoculated simultaneously than when the mold grew alone. The pH of mixed culture of Leu. mesenteroides and Lac. plantarum showed significantly lower value than the pH of the pure culture of A. parasiticus (p<0.05). The acidity of the mixed culture group significantly increased compared with the control group (A. parasiticus alone group) (p<0.05). The aflatoxin $B_1$ production was significantly decreased in the mixed culture group than in the A. parasiticus alone group (p<0.05). Leu. mesenteroides showed the more efficient effect than Lac. plantarum. These results indicate that the two lactic acid bacteria have inhibitory effect on the growth and aflatoxin $B_1$ production of Aspergillus parasiticus.