• Korean Journal of Microbiology
• /
• v.19 no.1
• /
• pp.14-22
• /
• 1981

Protein content of cellulosic wastes, such as spent grain, hop bark, spent rye, rice straw, rice hull, saw dust and used newspaper, was increased by a mixed culture of C. utilis wastes having 66-75% moisture. Among the fungal strains tested. A.phoenicis KU175 was the most powerful to increase the protein content of A. phoenicis during the mixed culture with C. utilis in the CMC medium reached at the peak for one day culture after inoculation of the both strains at the same time, while it reached at peark from the beginning of the mixed culture, when A. phoenicis was inocultated for 12-24hours prior to the inoculation of C.utilis. To increase the protein content of the cellulosic wastes by the mixed culture of C.utilis and A.phoenicis, the inoculation of both strains at the same time was more effective than the preinoculation of A. phoenicis for 6-24 hours. Content of crude cellulose in the used newspaper, saw dust and spent grain was high relatively, and the lignin content of spent grain, spent rye, and rice strew was reduced more than half by the treatment of 2% NaOH. However, effect of alkali treatment of increase the protein content of the cellulosic wastes was not prominent in the case of mixed culture. Protein content of the cellulosic wastes was increased prominently by the mixed culture of C.utilis and A.phoenicis in semi-solid substrate, compared with the single culture of C. utilis, although the latter increased the protein content of cellulosic wastes considerably. The effect of mixed culture of C. utilis and A. phoenicis increased 4-fold the protein content of spent grain, and more than doubled crude protein in hop bark and rice straw.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.40 no.9
• /
• pp.1300-1306
• /
• 2011

This study investigated the physicochemical characteristics of wet-milled rice flour treated with pectinase and cellulase in a steeping process. Enzyme treatments were used as follows: pectinase 0.05%, cellulase 0.05%, and mixed enzyme treatments 0.05~0.2%. For particle distribution, rice flour E-treated with mixed enzymes (pectinase 0.05% and cellulase 0.05%) was the finest at 48.3% particle distribution less than $53\;{\mu}m$. Protein contents and damaged starch were reduced by enzyme treatments. Damaged starch was the lowest (12.1%) in rice flour E compared with non-enzyme treatment (18.1%). Amylose content, water binding capacity, solubility, and swelling power all increased upon enzyme treatments, and their effects increased upon mixed enzyme treatment. For gelatinization characteristics of RVA, peak viscosity, final viscosity, breakdown, and total setback viscosity increased in rice flours treated with mixed enzymes. Especially, in steeping method with mixed enzyme treatment, pectinase 0.05% and cellulase 0.05% treatment was suitable for minimizing damaged starch and high fine particle distribution of rice flours compared with single enzyme treatment.

• The Journal of Internal Korean Medicine
• /
• v.38 no.6
• /
• pp.863-876
• /
• 2017

Objectives: The aim of this experimental study was to evaluate the anti-neoplastic and anti-inflammatory effects of single and mixed extracts of Ulmus davidiana (UD) and Oldenlandia diffusa (OD) on azoxymethane/dextran sodium sulfate (AOM/DSS)-induced colonic neoplasms in mice. Methods: AOM/DSS induces colitis-associated colonic neoplasms in mice. Mice were divided into seven groups: normal-no inducement and no treatment; control-colonic neoplasms with no treatment; UD-colonic neoplasms and treatment with UD; OD-colonic neoplasms and treatment with OD; UD1+OD1-colonic neoplasms and treatment with UD1 and OD1. UD1+OD2-colonic neoplasms and treatment with UD1 and OD2; UD2+OD1-colonic neoplasms and treatment with UD2 and OD1. Single and mixed preparations of UD and OD were applied to mice for six weeks. The colon length and weight and histopathologic changes of colon tissue were observed. Serum pro-inflammatory cytokines, including tumor necrosis factor-alpha ($TNF-{\alpha}$) and interleukin-6 (IL-6), were determined by enzyme-linked immunosorbent assay. The mRNA expression levels of Bax, Bcl-2, and interferon-gamma ($INF-{\gamma}$) were measured by RT-PCR. Results: The colon length was significantly increased in OD, UD1+OD2, and UD2+OD1 mice, and the colon weight was significantly decreased in OD and UD1+OD2 mice. The morphological change of colon epithelial cells was more suppressed in complex-treatment groups than in single-treatment groups. The inhibitory effect on inflammatory cell invasion was especially shown in UD1+OD2 mice. The serum level of the pro-inflammatory $TNF-{\alpha}$ was decreased in all complex-treatment groups, and the IL-6 level was decreased in UD1+OD1 mice. Single-treatment groups had an increase in the mRNA expression of the pro-apoptosis regulator Bax, and UD2+OD1 decreased the mRNA expression of the anti-apoptosis regulator Bcl-2. The mRNA expression of $INF-{\gamma}$ associated with inflammation was decreased in OD and UD1+OD2 mice. Conclusions: This study suggests that single and mixed extracts of Ulmus davidiana and Oldenlandia diffusa have anti-neoplastic and anti-inflammatory effects on AOM/DSS-induced colonic neoplasms in mice. Therefore, we conclude that UD, OD, and a mixture of UD and OD are potential therapeutic agents for colitis-associated colonic neoplasms.

• Journal of the Korean Society for Heat Treatment
• /
• v.31 no.3
• /
• pp.104-110
• /
• 2018

The effects of heat treatment time and cooling method on microstructure and mechanical property of Cu-22wt%Sn alloy were discussed. ${\alpha}+{\delta}$ mixed phase structure was obtained in air-cooled specimens after heat treatment at 775, 750, and $700^{\circ}C$ for 1 hour. On the other hand, in water-cooled specimens, ${\alpha}+{\beta}^{\prime}$ martensite mixed phase was obtained. In the case of water-cooled specimens, the hardness value decreased with decreasing heat treatment temperature because the volume fraction of ${\alpha}$ phase with low hardness value increased as the heat treatment temperature decreased. In water-cooled specimen after heat treatment at $600^{\circ}C$, ${\gamma}^{\prime}$ martensite was formed instead of ${\beta}^{\prime}$ martensite. The hardness value of ${\gamma}^{\prime}$ martensite was lower than those of ${\beta}^{\prime}$ and ${\delta}$ phases.

• Korean Journal of Environmental Agriculture
• /
• v.16 no.4
• /
• pp.285-290
• /
• 1997

This study was conducted to develop labor-saving pesticide-fertilizer mixed formulation through a single application of mixed formulation of an insecticide imidacloprid coated on a slow release urea. The release of both total nitrogen and imidacloprid into water was delayed with increasing adhesive.Imidacloprid was released out 95% within 24 hours, whereas total nitrogen released in mixed formulation until 40 days was released less extent by increasing adhesive. There was no difference between pesticide-fertilizer mixed formulation and coated urea thereafter. The content of $NH_4-N$ in the soil treated with pesticide and urea seperating was higher until 10 days but gradually decreased compare to that of pesticide-fertilizer mixed formulation or coated urea. The population density of nitrate reducing bacteria and denitrifying bacteria in soil after treatment were lower in the pesticide-fertilizer mixed formulation and coated urea than those of pesticide and urea separate treatment. The residue of imidacloprid in soil was slightly higher in the treatment of pesticide alone than the pesticide-fertilizer mixed formulation and pesticide and urea seperating treatment. The population and control efficacy of small brown planthopper, Laodelphax striatellus, were not significance different among treatments.

• Journal of the Korean Society of Clothing and Textiles
• /
• v.30 no.1 s.149
• /
• pp.115-124
• /
• 2006

Natural dyes are environmentally and human compatible. But they are not various or not fast in color. The mixed dyeing have been attempted to solve these disadventages of natural dyes. The persimmon juice dyed fabrics have brown-color and good hygienic properities however low color fastness. The onion dyeing show similar brown-color and have good color fastness caused by querectin existed in onion outer skins. Mixed dyeing was carryied out on silk fabrics using persimmion juice and onion outer skin extract in this study. The mixing method were persimmon juice dyeing and then onion dyeing(P-O), onion dyeing and then persimmon juice dyeing(O-P) and dyeing in the mixture of persimmon juice and onion outer skin extract simultaneously(P+O). The mordants were none-mordent, gallic acid after-treatment and alum after-treatment. Several persimmon juice dyed fabrics were irradiated for 2 hours before onion dyeing(PU-O). The color values of dyed silk fabrics were as follows. The persimmon juice dyed silk fabric developed to yellow-red color after 2 hours of uv irradiation. Onion dyed fabrics show similar yellow-red color after dyeing without uv irradiation. The effect of alum after-treatment on color difference were highest in onion dyeing. The dyeabilities of both P-O and O-P were higher than persimmom juice dyeing and onion dyeing. The dyeabilities of P+O was lower than persimmom juice dyeing and onion dyeing. The value of color difference of alum-treated fabric was the highest. The color difference of P-O and O-P caused from 2 to 4 hours of uv-irradiation were lower than those of persimmon juice dyed fabrics. Onion skin extract could prevent the color-change of persimmon juice dyed fabrics in mixed dyeing. The color difference of PU-O was higher than the P-O.

• Journal of Physiology & Pathology in Korean Medicine
• /
• v.19 no.2
• /
• pp.475-480
• /
• 2005

This study is designed to find out the effect of Corydalidis Tuber Carthami Flos Paeoniae Radix Rubra mixed water extract on adjuvant arthritis, and to check the stability for Corydalidis Tuber Carthami Flos Paeoniae Radix Rubra mixed water extract about the liver. At first, rats were devided into four groups; Normal(Non-treated group with FCA), Control(Group administrated saline everyday for 1 week after treatment of FCA), Sample A(group administrated Corydalidis Tuber Paeoniae Radix Rubra Carthami Flos mixed water extract $9mg/200{\sim}220g$ everyday 1 week after treatment of FCA) and Sample B(Group administrated Corydalidis Tuber Paeoniae Radix Rubra Carthami Flos mixed water extract $18mg/200{\sim}220g$ everyday 1 week after treatment of FCA). After then, separated searches were made on the change of edema rate, the number of WBC, the contented quantities of total protein, total bilirubin, GOT, GPT in the serum. Result : In the right planter edema, both of Sample groups showed a decrease with statistical significance in comparison with control group. In the WBC count, Sample B group showed a decrease with statistical significance in comparison with control group and also sample A group showed a decrease, but did not show statistical significance. In the serum total protein, both of samples did not show statistically significant decrease in comparison with the control group. In the serum total bilirubin, GOT and GPT, both of samples did not show any harmful change about the liver in comparison with the control group. According to the above results, it is concluded that Corydalidis Tuber Paeoniae Radix Rubra Carthami Flos mixed water extract had the therapeutic effect of anti-inflammation in adjuvant arthritis with no injury to liver.

• Journal of the Korean Society for Heat Treatment
• /
• v.12 no.1
• /
• pp.47-54
• /
• 1999

Microstructure of Al-Si coated PWA 1426 alloy was studied. Diffusion coated specimens were heat treated for 4hr at $870{\sim}1087^{\circ}C$ and then were examined the changes of microstructure and interfacial compound by optical microscopy, SEM and EDS. According to the result of EDS, it is supposed that the coated layer was composed of $Ni_2Al_3$. When diffusion treatment was conducted at $1087^{\circ}C$, coated layer varied from $Ni_2Al_3$ to NiAl phase and composed of mixed, denuded and inter-diffusion layer.

• Journal of the Korea Organic Resources Recycling Association
• /
• v.29 no.3
• /
• pp.41-48
• /
• 2021

This study aimed to develop an alternative organic fertilizer to castor oil cake-based fertilizers. To assess the nutrient effect of the developed fermented mixed organic fertilizers, the yield of lettuce and soil characteristics after growth were analyzed and compared to those of a trial using a mixed expeller cake fertilizer. Two fermented mixed organic fertilizers, FA and FB, each containing 5.0% nitrogen, 2.6% phosphate, and 1.4% potassium, were produced by mixing different ratios of rice bran, dried distillers grains, sesame oil meal, and fish meal. This study was conducted with six trials: untreated, mixed expeller cake fertilizer, and the fermented mixed organic fertilizers FA and FB. Based on the amount of nitrogen fertilization (70 kg ha^-1) on the lettuce, the fermented mixed organic fertilizers FA and FB were applied at 100% and 150%, respectively, and the mixed oil cake was applied at 100%. As the amount of treatment increased, there was no significant difference except the number of leaves in FA treatment. The yields from the FA100 and FB100 treatments were 38.2 and 40.8 Mg ha^-1, respectively, which was not significantly different from that of the mixed expeller cake fertilizer treatment at 38.3 Mg ha^-1. In addition, the nitrogen uptake and utilization efficiency of the lettuce were not significantly different between mixed expeller cake fertilizer and fermented mixed organic fertilizer treatments. Analysis of the chemical properties of the soil after the trial showed that he mixed expeller cake fertilizer treatment showed the lowest pH. There were no significant differences in electrical conductivity, content of soil organic matter, available phosphate, and exchangeable cation among the fertilizer treatments. However, the bacterial and actinomyces density was higher in the soil from the fertilizer trials than in the non-fertilizer trials. These results indicated that the two tested fermented mixed organic fertilizers had nourishing effects and soil characteristics that were similar to those of the mixed expeller cake fertilizer. Thus, farmers can use these fermented mixed organic fertilizers as alternatives to castor oil cakes for the cultivation of organic lettuce.

• Journal of Microbiology and Biotechnology
• /
• v.6 no.2
• /
• pp.116-119
• /
• 1996

The biological treatment by a mixed culture GE2 immobilized on activated carbon was investigated with a phenolic resin industrial wastewater containing 41,000 mg/l of phenol and 2,800 mg/l of formaldehyde. At a dilution of 20 times with aerated tap water, influent and effluent $COD_{Mn}$ were 4,587 mg/l and 46 mg/l, that is, $COD_{Mn}$ removal efficiency was 99.0%. At this time, phenol and formaldehyde con-centration of the effluent were 1.24 and 6.80 mg/l, indicating removal efficiencies of 99.9 and 94.1%, respectively.