• Asian-Australasian Journal of Animal Sciences
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• 제22권2호
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• pp.201-205
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• 2009

Effects of Cordyceps militaris mycelia on rumen microbial fermentation were determined by measuring in vitro gas production, cellulose digestion and VFA concentrations. C. militaris mycelia was added to buffered rumen fluid with final concentrations of 0.00, 0.10, 0.15, 0.20, 0.25 and 0.30 g/L and incubation times were for 3, 6, 9, 12, 24, 36, 48 and 72 h. At all incubation times, the gas production showed a quadratic increase with the supplementation of C. militaris mycelia; maximum responses were seen with 0.25 g/L supplementation. However, the gas production was significantly lower for the 0.30 g/L supplementation than for the 0.25 g/L supplementation from 9 h to 72 h incubation. The cellulose filter paper (FP) digestion showed a quadratic increase, as did the gas production except at 3 h incubation. The concentration of total VFA was significantly increased by the supplementation of C. militaris mycelia compared with the control treatment; the highest response was also seen with 0.25 g/L supplementation. This was true for responses in the concentration of acetic and propionic acids. As opposed to other responses, the responses of pH to the supplementation of C. militaris mycelia showed a quadratic decrease from 3 h to 36 h incubation. In conclusion, C. militaris mycelia alter the mixed rumen microbial fermentation with increases in the production of gas and VFA, and cellulose FP digestion.

• 유기물자원화
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• 제3권1호
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• pp.3-11
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• 1995

음식물찌꺼기를 $1.1m^3$ 규모로 퇴적하여 강제 송풍방식으로 80일 이상 호기적 퇴비화를 실시하였으며, 퇴비화기간 동안 몇가지 이화학적인 특성을 조사하였다. 음식물찌꺼기의 퇴비화 과정중 색도 및 탄질율의 변화를 분석한 결과, 음식물찌꺼기의 유기성 물질들은 신속하게 분해되어 약 30일이면 안정화에 도달하는 것으로 보인다. 그러나 pH는 40일 이후에 중성범위에서 안정화되고, 원형여지크로마토그램은 45일 이후에 특정적인 변화가 나타났으며, EC값은 50일이 경과한 후 안정화에 도달하는 것으로 평가되었다. 이러한 결과들은 음식물찌꺼기를 이용한 퇴비의 안정화 평가를 위해서는 몇가지 이화학적인 특성을 종합적으로 고려해야 함을 의미하는 것으로 판단된다.

• 대한수의학회지
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• 제54권3호
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• pp.139-146
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• 2014

Jeotgal (salted seafood) has been one of major fermented foods in Korea for long time. Although there are many studies about Jeotgal in various aspects of food, its immunological importance on hosts has not been elucidated yet. In this study, we investigated if several bacteria isolated from Jeotgal may modulate the function of dendritic cells (DCs), powerful antigen-presenting cells equipped with special immunological capabilities. 4 Jeotgal bacteria were selected as representatives and used for experiments. To treat viable DCs, those bacteria were killed at $60^{\circ}C$ for 30 min. The viability of DCs treated with Jeotgal bacteria was verified and two isolates significantly induced high production of interleukin-12, a representative cell-mediated cytokine of DCs. Surface activation and maturation markers (MHC class II, CD40, CD86) of DCs were analyzed by flow cytometer. In addition, the treated DCs showed significantly high lymphocyte stimulatory capability compared to control DCs based on allogeneic mixed lymphocyte reactions. These observations suggest that Jeotgal isolates can function as immunostimulating bacteria in hosts, like Lactobacillus. Taken together, these experimental evidences may broaden the use of Jeotgal isolates in immunological fields in addition to as a fermented food.

• Applied Biological Chemistry
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• 제33권2호
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• pp.133-137
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• 1990

담수토양중(湛水土壤中)에 있어서 IBP의 분해(分解)에 미치는 각종(各種) 토양환경조건(土壤環境條件)의 영향(影響)에 대하여 연구검토(硏究檢討)하였다. IBP는 비담수조건(非湛水條件)에서 보다 담수조건(湛水條件)에서 느리게 분해(分解)되었다. 분해속도(分解速度)는 토양종류(土壤種類)에 따라 크게 변화(變化)되었으며, 유기물함양(有機物含量)이 높은 토양(土壤)에서보다 낮은 토양(土壤)에서 빨랐다. IBP는 저온(低溫)에서보다 고온(高溫)의 토양(土壤)에서, 고농도(高濃度)에서보다 저농도(低濃度) 첨가(添加)에서 빨리 분해(分解)되었다. 토양중(土壤中) 분해속도(分解速度)는 볏짚첨가에 의하여 현저히 저하(低下)되었으나, 복합비료(複合肥料)나 타농약(他農藥) fenitrothion과 butachlor의 첨가(添加)에 의하여는 영향(影響)을 받지 않았다. 토양중(土壤中) IBP는 호기적(好氣的) 미생물(微生物)에 의하여 분해(分解)되는 것으로 추정(推定)되었다.

• 한국식품과학회지
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• 제9권3호
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• pp.199-204
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• 1977

경기도 이천(利川)에서 수확한 고추(새마을 금장고추 2호)를 건조제분하여 평형수분(平衡水分)을 측정하였으며 125, 250, 500, 1,000 krad의 Co-60 감마선을 조사(照射) 후 저장 중 capsaicin, capsanthin 및 미생물 수의 변화를 조사하여 다음과 같은 결과를 얻었다. 1) $25^{\circ}C$에서 고추의 평형수분함량은 상대습도 43.7, 53.3, 64.4, 75.4, 84.3%에서 각각 13, 18, 25, 28, 37%이었다. 2) 제분도(製粉度)가 낮을수록, 즉 고추가루의 입경(粒徑)이 클수록 평형수분함량이 낮아 장기저장에 유리하였다. 3) 고추가루중의 생존(生存)미생물 수는 g당 약 $10^8$개나 되었으며 혼합미생물의 $D_{10}$ 값은 210 krad이었다. 4) 수분, capsanthin은 500, 1,000 krad 조사선량(照射線量)에서 저장중 변화가 거의 없었으나 capsaicin은 방사선조사(照射)에 의하여 부분적으로 파괴되었다.

• 한국축산식품학회지
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• 제32권1호
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• pp.1-5
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• 2012

The objective of this study was to examine the effects of a combined treatment regarding antimicrobial food ingredients and high pressure processing (HP) on the inactivation efficiency of Escherichia coli and Listeria monocytogenes inoculated into ground pork. Ethanol extracted from garlic, leeks, onions, and ginger powder was prepared. Half of the prepared powder was irradiated at 5 kGy to see the effect of pasteurization before addition. The prepared food ingredients were added into radiation-sterilized ground pork (1%, w/w), and inoculated with E. coli and L. monocytogenes. The samples were vacuum-packed and applied with HP at 0.1 (control), 300, 450, and 600 MPa. Microbial log reduction increased with the increase of pressure up to 600 MPa. With minor exceptions, overall efficiency of HP treatment with regards to inactivation of pathogens increased. Inoculated microorganisms showed approximately 7-8 Log reductions by 600 MPa, except for L. monocytogenes treated with garlic (5.7 Log reductions). The E. coli reduction in ground pork mixed with ethanol extracted garlic showed the highest efficiency (1.86) compared to leeks (1.25-1.31), onions (1.17-1.44), and ginger (1.50-1.82) when treated at an HP of 450 MPa. There was no evidence for the advantage of pasteurization concerning the food ingredients before addition of antimicrobial food ingredients and HP. Results demonstrate that the combination of antimicrobial food ingredients and HP treatment may help improve the efficiency of sterilization in meat systems.

• 한국축산식품학회지
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• 제22권2호
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• pp.164-171
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• 2002

박테리오신을 생산하는 9종의 유산균 배양액으로부터 ammonium sulfate를 첨가하여 제조된 각각의 조박테리오신을 혼합하여 복합박테리오신용액을 제조하였다. 복합박테리오신의 항균 활성은 단일 박테리오신보다 우수하였으며 항균 범위 또한 넓어짐을 알 수 있었고 단일 박테리오신에 대하여 저항성을 나타내는 Listeria monocytogenes의 생육을 저해하는 것으로 나타났다. 또한, 복합박테리오신은 pH와 열에강한 안정성을 보여 식품 가공 중에도 이용이 가능한 것으로 나타났다. 복합박테리오신을 프랑크소세지, 모짜렐라 치즈 및 돈육등심근에 첨가한 후 저장기간별 일반세균수의 변화를 조사한 결과, 대조구에 비해 유의적인 감소현상을 나타내었고 저장 28일 경과 후에는 모든 식품에서 1/10 또는 1/100 정도로 식품내 총세균이 억제되었다. 또한, 저장기간 중 VBN의 함량을 조사한 결과 돈육등심근과 모짜렐라 치즈의 경우 14일 이후부터 박테리오신 처리구가 대조구에 비해 유의적으로 낮은 수치를 보여주었다.

• Journal of Microbiology
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• 제33권3호
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• pp.208-214
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• 1995

The effects of various 2, 4-D-degradative plasmids on the axenic growth patterns, the degradation phenotypes, and the competitiveness of different host bacteria were evaluated in liquid cultures; the organisms and plasmids used were Alcaligenes eutrophus JMP134/pJP4, Alcaligenes paradoxus/p2811, Pseudomonas pickettii/p712, pJP4, and p712 or p 2811 exhibited very different restriction fragment profiles in restriction endonuclease digests. These plasmids were transferred to the recipients (P. cepacia and Alcaligenes JMP228) at relatively high frequencies ranging from 8.9 $\times$ 10$^3$ to 1.6 $\times$ 10$^5$ per donar cell. In the axenic liquid cultures the fast-growing strains, such as P. pseudomallei/p745 and P. cepacia/pJP4, exhibited short lag periods, high specific growth rates, and high relative fitness coefficients, while the slow-growing strains, such as P. pickettii/p712 and A. paradoxus/p2811, had long lag periods, low specific growth rates, and low relative fitness coefficients. Depending on the type of plasmid containing the genes for the 2, 4-D pathway, some transconjugants exhibited intermediate grwoth patterns between the fast-growing strains and the slow-growing strains. The plasmid and plasmid-host interactions determined specific growth rate and lag time, respectively, which were shown to be principal determinants of competitiveness among the strains, but relative fitness coefficient derived from the axenic culture was not always predictive for the mixed culture condition.

• 한국식품저장유통학회지
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• 제11권1호
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• pp.17-21
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• 2004

본 실험에서는 식품의 미생물작용에 의한 변질의 저해 효과가 뛰어난 식물성천연항균제품(botanical antimicrobial agent)에 천연보조제(인삼추출물, 알로에, 매실추출물)를 첨가하여 paper disk법에 의한 항균성 검사를 실시한 결과, 인삼추출물을 첨가한 천연복합항균제재(BAAG)에서 생육저해환이 가장 크고 뚜렷하게 나타났다. 한편, 천연 항균복합제재 용액에 콩나물용 콩을 농도별로 각각 침지 처리하여 냉장 및 상온에 저장하면서 대장균수, 총균수의 변화를 살펴보고, 외관상의 변화에 미치는 영향을 수도수로 침지처리한 대조구와 비교하면서 조사하였다. 실험 결과, 대장균은 BAAG의 침지처리에 의해 성장이 억제되었으며 처리농도가 증가시킬수록 균증식 억제 효과가 뚜렷하였고, 총균수도 BAAG 처리구의 콩나물이 가장 낮은 수치를 나타내었다. 외관상의 변화와 상품 가치를 측정한 결과는 수도수처리구인 대조구 콩나물이 저장 3일 후부터 부패취와 함께 표피의 점질성 갈변이 형성되어 상품가치가 크게 떨어진 상태였다. 위와 같은 결과, BAAG용액 속에서 콩나물 콩을 발아시켜 침지 처리함으로써 콩나물의 유통기간을 상당기간 연장할 수 있을 것으로 기대된다.

• 한국지하수토양환경학회지:지하수토양환경
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• 제24권4호
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• pp.1-10
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• 2019

Recently, groundwater contamination by mixed occurrence of arsenic (As) and nitrate ($NO_3{^-}$) has been a serious environmental issue all around world. In this study, we investigated the microbial As(III) oxidation characteristic under denitrification process to examine the feasibility of the microbial consortia in wetland sediment to simultaneously treat these two contaminants. The detail objectives of this study were to investigate the effects of $NO_3{^-}$ on the oxidation of As(III) in anaerobic environments and observe the microbial community change during the As oxidation under denitrification process. Results showed that the As(III) was completely and simultaneously oxidized to As(V) under denitrification process, however, it occurred to a much less extent in the absence of sediment or $NO_3{^-}$. In addition, the significant increase of As(III) oxidation rate in the presence of $NO_3{^-}$ suggested the potential of As oxidation under denitrification by indigenous microorganisms in wetland sediment. Genera Pseudogulbenkiania, and Flavisolibacter were identified as predominant microbial species driving the redox process. Conclusively, this study can provide useful information on As(III) oxidation under denitrifying environment and contribute to develop an effective technology for simultaneous removal of As(III) and $NO_3{^-}$ in groundwater.