• 한국식품과학회지
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• 제54권4호
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• pp.398-402
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• 2022

본 연구에서는 폴리페놀 함유 블랙 초크베리가 콜레스테롤 대사에 미치는 영향을 HepG2 세포에서 콜레스테롤 대사 관련 유전자 발현을 측정함에 따라 조사하였다. 블랙 초크베리는 콜레스테롤 대사와 관련하여 콜레스테롤 흡수, 생합성, 유출과 관련된 유전자 발현을 조절하는 것으로 나타났다. 이는 블랙 초크베리의 혈중 콜레스테롤 저하 효과가 콜레스테롤 및 담즙 대사 관련 유전자 발현을 조절함에 의한 것으로 사료된다. 추후 블랙 초크베리 내 어떠한 생리활성물질이 콜레스테롤 대사 유전자 발현을 조절하여 이러한 효과를 나타내는 지에 대한 연구가 필요하며, 블랙 초크베리의 콜레스테롤 저하 효과를 동물 및 임상에서 기전 연구를 진행하여 천연 유래 기능성 소재로서의 블랙 초크베리의 중요성을 검증할 필요가 있을 것으로 사료된다.

• Journal of electromagnetic engineering and science
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• 제15권3호
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• pp.142-150
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• 2015

Several reports supported that continuous exposure to 60 Hz magnetic field (MF) induces testicular germ cell apoptosis in vivo. We recently evaluated duration- and dose-dependent effects of continuous exposure to a 60 Hz MF on the testes in mice. BALB/c male mice were exposed to a 60 Hz MF at $100{\mu}T$ for 24 hours a day for 2, 4, 6, or 8 weeks, and at 2, 20 or $200{\mu}T$ for 24 hours a day for 8 weeks. To induce the apoptosis of testicular germ cell in mice, the minimum dose is $20{\mu}T$ at continuous exposure to a 60 Hz MF for 8 weeks, and the minimum duration is 6 weeks at continuous exposure of $100{\mu}T$. Continuous exposure to a 60 Hz MF might affect duration- and dose-dependent biological processes including apoptotic cell death and spermatogenesis in the male reproductive system of mice. The safety guideline of the International Commission on Non-Ionizing Radiation Protection (ICNIRP) indicates that the permissible maximum magnetic flux density for general public exposure is $200{\mu}T$ at 60 Hz EMF (ICNIRP Guidelines, 2010). In the present study, we aimed to examine the expression of pro- and anti-apoptotic genes regulated by the continuous exposure to 60 Hz at $200{\mu}T$ in Sprague-Dawley rats for 20 weeks. The continuous exposure to 60 Hz at $200{\mu}T$ does not affect the body and testicular weight in rats. However, exposure to 60 Hz MF significantly affects testicular germ cell apoptosis and sperm count. Further, the apoptosis-related gene was scrutinized after exposure to 60 Hz at $200{\mu}T$ for 20 weeks. We found that the message level of endonuclease G (EndoG) was greatly increased following the exposure to 60 Hz at $200{\mu}T$ compared with sham control. These data suggested that 60 Hz magnetic field induced testicular germ cell apoptosis through mitochondrial protein Endo G.