• 고려인삼학회:학술대회논문집
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• 고려인삼학회 1990년도 Proceedings of International Symposium on Korean Ginseng, 1990, Seoul, Korea
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• pp.168-174
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• 1990

This study was focused on the characterization of mitochondrial DNA (mtDNA) for molecular genetically approach of energy Production related mechanism in Panax Ein.fend. The simple and efficient method of mtDNA isolation from ginseng has been developed by modification of recently advanced methods. This procedure can successfully apply to mtDNA isolation of several plants. MtDNA of etiolated shoot and one-year root were digested with restriction endonucleases, but that of 6-year root not Any difference was not observed in the restriction endonuclease digestion patterns among the ginseng variants. Molecular size of ginseng mtDNA was estimated at least 159 kb by the restriction endonuclease fragment analysis. The 4.5 kb extra band at the lane of EcoRll treatment could be observed in restriction patterns digested with the methylation sensitive endonucleases, BstN 1 and EcoRll. For construction of mitochondrial genomic library of ginseng, mtDNA was partially digested with EcoRl, and packaged with EMBL4 phage vector Genomic library was screened and purified for further research including restricttion mapping of ginseng mtDNA, and cloning of the genes. The gene of ATP synthase A subunit was cloned koto the purified EMBL4 library clone No. 16. Now, clone No. 16 is subcloned for structure gene sequence analysis.

• Animal Systematics, Evolution and Diversity
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• 제33권3호
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• pp.169-175
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• 2017

In the northwestern Pacific region, the pen shell (Atrina pectinata) is a widely distributed bivalve and economically important in fisheries. Recently, stock of this species has been greatly reduced due to overexploitation and marine pollution, which arouses interest in conservation. Studies on genetic and taxonomic entities of pen shells have not been tried in Korea, which makes difficult to take measures for effective conservation of this marine resource. In this study, we investigated mitochondrial genetic polymorphism of pen shells collected from 4 locations in Korea and Japan using cytochrome c oxidase I (COI) gene sequences. A total of 39 haplotypes were identified among 86 individuals of pen shell. Although only 5 haplotypes were shared, no significant genetic differentiation was observed between Korean and Japanese populations. These results suggest that pen shell populations of these regions share an ancestral population which might have experienced expansion during the Pleistocene, but gene flow must have been highly restricted after expansion.

• Animal cells and systems
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• 제13권3호
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• pp.305-314
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• 2009

The control region of mitochondrial DNA (13516-14619) is located between srRNA and $tRNA^{lle}$ gene in swimming crab, Portunus trituberculatus. The present study was investigated the genetic polymorph isms of the control region in samples of P. trituberculatus collected at coastal waters of the Yellow Sea in Korea. A total of 300 substitution and indel polymorphic sites were identified. In addition to SNPs and indel variation, a hypervariable microsatellite motif was also identified at position from 14358 to 14391, which exhibited 10 alleles including 53 different suballeles. When the hypervariable microsatellite motif was removed from the alignment, 95 haplotypes were identified (93 unique haplotypes). The nucleotide and haplotype diversities were ranged from 0.024 to 0.028 and from 0.952 to 1.000, respectively. The statistically significant evidence for geographical structure was not detected from the analyses of neighbor-joining tree and minimum-spanning network, neither. This result suggest that population of P. trituberculatus are capable of extensive gene flow among populations. We believed that the polymorph isms of the control region will be used for informative markers to study phylogenetic relationships of P. trituberculatus.

• Journal of Ginseng Research
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• 제14권2호
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• pp.310-316
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• 1990

This study was focused on the characterization of mitochondrial DNA (mtDNA) for molecular 9enetical approach of energy Production related mechanism in Panax ginseng. The simple and efficient method of mtDNA isolation from ginseng has been developed by modification of recently advanced methods. This procedure can successfully apply to mtDNA isolation of several plants. mtDNA of etiolated shoot and one-year root were digested with restriction endonucleases, but that of 6-year root not. Any difference was not observed in the restriction endonuclease digestion patterns among the ginseng variants. Molecular size of ginseng mtDNA was estimated at least 159 kb by the restriction endonuclease fragment analysis. The 4.5 kb extra band at the lane of EcoRII treatment could be observed in restriction patterns digested with the methylation sensitive endonucleases, BstN I and EcoRII. For construction of mitochondrial genomic library of ginseng, mtDNA was partially digested with EcoRl, and packaged with EMBL4 phage vector. Genomic library was screened and purified for further research including restriction mapping of ginseng mtDNA, and cloning of the genes. The gene of ATP synthase A subunit was cloned from the purified EMBL4 library clone No. 16. Now, clone No. 16 is subcloned for structure gene sequence analysis.

• Animal Systematics, Evolution and Diversity
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• 제28권1호
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• pp.36-41
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• 2012

The larvae of Chaoborus are widely distributed in lakes, ponds, and reservoirs. These omnivorous Chaoborus larvae are crucial predators and play a role in structuring zooplankton communities, especially for small-sized prey. Larvae of Chaoborus are commonly known to produce predator-induced polyphenism in Daphnia sp. Nevertheless, their taxonomy and molecular phylogeny are very poorly understood. As a fundamental study for understanding the role of Chaoborus in predator-prey interactions in a freshwater ecosystem, the molecular identification and phylogenetic relationship of Chaoborus were analyzed in this study. A molecular comparison based on partial mitochondrial cytochrome oxidase I (COI) between species in Chaoborus was carried out for the identification of Chaoborus larvae collected from 2 localities in Korea. According to the results, the Chaoborus species examined here was identified as C. flavicans, which is a lake-dwelling species. Furthermore, partial mitochondrial genome including COI, COII, ATP6, ATP8, COIII, and ND3 were also newly sequenced from the species and concatenated 5 gene sequences excluding ATP8 with another 9 dipteran species were compared to examine phylogenetic relationships of C. flavicans. The results suggested that Chaoborus was more related to the Ceratopogonidae than to the Culicidae. Further analysis based on complete mitochondrial DNA sequences and nuclear gene sequences will provide a more robust validation of the phylogenetic relationships of Chaoborus within dipteran lineages.

• Animal cells and systems
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• 제16권4호
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• pp.313-320
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• 2012

Genetic variation was surveyed at four microsatellite loci and 1416 base pairs (bp) of the mitochondrial DNA (mtDNA) cytochrome c oxidase I gene (COI) to clarify the genetic structure of the small yellow croaker, Larimichthys polyactis, in the Yellow and East China Seas, especially regarding four provisional populations, (one Korean and three Chinese populations). Based on microsatellite DNA variations, the estimated expected heterozygosity ($H_E$) in each population ranged from 0.776 to 0.947. The microsatellite pairwise $F_{ST}$ estimates showed no significant genetic differentiation between the populations. MtDNA variations also indicated no genetic structure in L. polyactis, but very high variability. The absence of genetic differentiation among and within populations of L. polyactis may either result from the random migration of the adult or the passive dispersal of the eggs and larvae.

• 한국어류학회지
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• 제21권4호
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• pp.291-298
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• 2009

한국, 중국, 일본, 대만 등 동아시아에 분포하는 피라미속 (Zacco) 어류에 대한 mitochondrial cytochrome b gene의 염기서열 분석을 통해 분자계통학적 유연관계를 조사하였다. MP tree 분석 결과, 한국산 참갈겨니와 갈겨니 집단은 모두 단계통군을 형성하고 있었으나 생물분포구계의 남한아 지역에 분포하는 참갈겨니와 갈겨니 집단은 일본산 갈겨니와 유전적으로 유사하게 분석되었다. 또한 한국산 피라미 집단은 일본산 피라미와 유전적 친화성을 보였으나, 중국산 피라미는 끄리와 분자계통학적 유연관계를 형성하였다. 참갈겨니의 한강집단은 동해 중북부에 서식하는 집단과 유전적으로 유사하였고, 갈겨니의 동진강과 영산강 집단은 유전적 동일 집단, 동진강과 섬진강 집단은 유전적 유사집단으로 여겨졌다. NJ tree 분석을 통해 피라미 집단은 참갈겨니와 갈겨니 및 Z. sieboldii 집단과는 오래전에 공동조상으로부터 분화되었고, Z. sieboldii의 조상으로부터 참갈겨니와 갈겨니가 분기진화한 것으로 추정되었다. 또한 중국에 서식하는 피라미는 한국산 피라미 집단과 상당한 유전적 차이를 보였을 뿐만 아니라 끄리속 어류와 분자계통수를 형성하고 있어 추후 동물지리학적 분포 및 피라미속 어류에 대한 계통분류학적 논의가 요구된다.

• 한국패류학회:학술대회논문집
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• 한국패류학회 2005년도 전기 학술대회 초록집
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• pp.21-21
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• 2005

• 생명과학회지
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• 제26권11호
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• pp.1232-1236
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• 2016

Mitochondrial calcium uptake 1 (MICU1)은 2개의 canonical EF hands를 가지고 미토콘드리아 내막에 위치하여, 미토콘드리아의 칼슘 섭취에 중요한 기능을 하는 것으로 알려져 있다. 근육 세포의 미토콘드리아 칼슘 섭취는 사후에 급속냉각 또는 냉장 시, 근육 내 칼슘 방출로 인한 강직과 관련되어 있으므로 궁극적으로는 육질 형성에 관련이 있을 것으로 판단된다. 따라서 본 연구에서는 돼지 MICU1 유전자의 exon영역의 변이를 탐색하고, 발굴된 변이에 대해 육질 형질과의 연관성 분석을 실시하였다. 이를 위해 버크셔 667두(암퇘지 347두, 거세수퇘지 320두)가 이용되었으며, MICU1 유전자의 cDNA를 염기서열 해독하여 비교함으로써 exon 영역의 변이를 발굴하였다. 그 결과 MICU1의 3' 비해독 영역(untranslated region, UTR)에서 3개의 단일염기다형성(single-nucleotide polymorphism, SNP)를 발견했다. 그리고 이들 SNP에 대해 공시돈의 육질형질(근육 pH, 육즙 손실, 육색, 근내지방함량)과 연관성 분석을 실시했다. SNP1 (c.*136G>A)에서는 육즙 손실(p=0.017), 근내지방함량(p=0.039)과 연관되어 있었고, SNP2 (c.*222G>A)와 SNP3 (c.*485G>A)에서는 각각 육즙 손실(p=0.018)과 근내지방함량(p<0.001)과 연관되어 있는 것을 확인하였다. 따라서 본 결과를 바탕으로, 돼지에서 육질과 관련된 후보 유전자로 추정된 MICU1 유전자로부터 3' 비해독 영역의 변이가 유의적으로 육질 형질과 관련되어있다는 것이 확인되었다. 향후 MICU1 유전자의 3' 비해독 영역의 변이들에 기능적 역할을 정확히 파악하기 위한 분자생물학적 특성 연구가 필요할 것으로 판단된다.

• Genomics & Informatics
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• 제5권4호
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• pp.168-173
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• 2007

In previous nuclear genomic association studies, Random Forests (RF), one of several up-to-date machine learning methods, has been used successfully to generate evidence of association of genetic polymorphisms with diseases or other phenotypes. Compared with traditional statistical analytic methods, such as chi-square tests or logistic regression models, the RF method has advantages in handling large numbers of predictor variables and examining gene-gene interactions without a specific model. Here, we applied the RF method to find the association between mitochondrial single nucleotide polymorphisms (mtSNPs) and diabetes risk. The results from a chi-square test validated the usage of RF for association studies using mtDNA. Indexes of important variables such as the Gini index and mean decrease in accuracy index performed well compared with chi-square tests in favor of finding mtSNPs associated with a real disease example, type 2 diabetes.