• 한국응용곤충학회지
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• 제61권4호
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• pp.641-649
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• 2022

미토콘드리아 시토크롬 c 산화효소 1 (COX1) 유전자 염기서열(658 bp)을 사용하여, 콩 포장에서 채집된 어리팥나방(Matsumuraeses falcana)과 팥나방(Matsumuraeses phaseoli)의 종을 실험실 집단의 종들과 비교하여 동정하였다. COX1 염기서열 분석에서, 어리팥나방 47개체로부터 10개의 하플로타입이 발견되었고, 종내 유전적 거리는 0.15~0.46%이었다. 이중 하프로타입 A형이 약 70%로 우점형이었다. 팥나방의 30개체로부터는 모두 동일한 하나의 서열만이 확인되었고, 어리팥나방과의 종간 유전적 거리는 4.11~4.61%이었다. 두 종의 COX1 염기서열을 번역한 아미노산 서열은 모두 동일하여 동의적 염기서열 변이(동의치환, 同義置換, synonymous substitution)를 확인할 수 있었다. 포장 조사에서 두 종의 유충이 콩의 잎과 꼬투리를 가해하였고, 한 포장에서 동시에 발생하였다. 전체 포장에서 어리팥나방의 평균 밀도는 팥나방보다 약 1.5배 높았다. 이 결과는 콩이 두 종의 동일 기주임을 명백하게 제시하였다. 별도로 이 속의 유충 기생파리로서 Elodia flavipalpis (파리목: 기생파리과)가 발견되었고, COX1 서열로 동정되었다.

• Parasites, Hosts and Diseases
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• 제56권1호
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• pp.87-91
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• 2018

Morphological and molecular characteristics of spirometrid tapeworms, Spirometra decipiens, were studied, which were recovered from a heavily infected stray cat road-killed in Eumseong-gun, Chungcheongbuk-do (Province), the Republic of Korea (=Korea). A total of 134 scolices and many broken immature and mature proglottids of Spirometra tapeworms were collected from the small intestine of the cat. Morphological observations were based on 116 specimens. The scolex was 22.8-32.6 mm (27.4 mm in average) in length and small spoon-shape with 2 distinct bothria. The uterus was coiled 3-4 times, the end of the uterus was ball-shaped, and the vaginal aperture shaped as a crescent moon was closer to the cirrus aperture than to the uterine aperture. PCR amplification and direct sequencing of the cox1 target fragment (377 bp in length and corresponding to positions 769-1,146 bp of the cox1 gene) were performed using total genomic DNA extracted from 134 specimens. The cox1 sequences (377 bp) of the specimens showed 99.0% similarity to the reference sequence of S. decipiens and 89.3% similarity to the reference sequence of S. erinaceieuropaei. In the present study, we report a stray cat heavily infected with S. decipiens identified by mitochondrial cox1 sequence analysis and morphological examinations of the adult worms.

• Animal Systematics, Evolution and Diversity
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• 제34권4호
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• pp.215-219
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• 2018

Protochitonidae Ashby, 1925 is a family of small to medium sized chitons that includes a single fossil genus and two extant genera. Of the two extant genera, Deshayesiella Carpenter in Dall, 1879 contains 5 described species. Although most Deshayesiella species are known to be found in deep sea habitats(over 100 m), D. curvata (Carpenter in Pilsbry, 1892) is found from shallow waters(1-20 m). In this study, we provide details of microstructure of shell and radula characters using scanning electron microscopy and morphological features of D. curvata, and its partial sequence of mitochondrial DNA cox1 gene as DNA barcode sequence. In addition, we compare morphological differences of D. curvata from other congeneric species.

• Parasites, Hosts and Diseases
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• 제56권2호
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• pp.195-198
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• 2018

Six cystic metacestodes were found in the abdominal muscles of a wild rabbit, Lepus sinensis, in China. The coenurus contained one or more scolices armed with hooklets. Mitochondrial cox1 (1,623 bp) confirmed 98% homology with cox1 of Taenia serialis. This is the first report of T. serialis infection in an intermediate host in the Qinghai Tibetan Plateau Area, China.

• ALGAE
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• 제30권3호
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• pp.217-222
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• 2015

Pythium species (Pythiales, Oomycetes) are well known as the algal pathogen that causes red rot disease in Pyropia / Porphyra species (Bangiales, Rhodophyta). Accurate species identification of the pathogen is important to finding a scientific solution for the disease and to clarify the host-parasite relationship. In Korea, only Pythium porphyrae has been reported from Pyropia species, with identifications based on culture and genetic analysis of the nuclear internal transcribed spacer (ITS) region. Recent fungal DNA barcoding studies have shown the low taxonomic resolution of the ITS region and suggested the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene as an alternative molecular marker to identify Pythium species. In this study, we applied an analysis of both the ITS and cox1 regions to clarify the taxonomic relationships of Korean Pythium species. From the results, the two closely related Pythium species (P. chondricola and P. porphyrae) showed the same ITS sequence, while the cox1 marker successfully discriminated P. chondricola from P. porphyrae. This is the first report of the presence of P. chondricola from the infected blade of Pyropia yezoensis in Asia. This finding of the algal pathogen provides important information for identifying and determining the distribution of Pythium species. Further studies are also needed to confirm whether P. chondricola and P. porphyrae are coexisting as algal pathogens of Pyropia species in Korea.

• Journal of Species Research
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• 제13권3호
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• pp.332-339
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• 2024

The filamentous freshwater red alga Compsopogon caeruleus(Compsopogonophyceae, Compsopogonaceae, Rhodophyta) occurs in tropical and subtropical regions of worldwide. This species has been reported from Asia, America, Africa, Europe and Oceania, and the worldwide distribution of Compsopogon caeruleus is in variable water habitats. Several morphospecies of the genus Compsopogon had been recorded, but recent molecular phylogenetic analyses with worldwide sampling identified a monospecific genus, C. caeruleus. In the present study, we first report a freshwater red alga Compsopogon caeruleus from Korea. We identified Compsopogon caeruleus in an urban river in Yongin City, and analyzed its morphological and genetic characteristics. Nuclear 18S rDNA, plastidal rbcL gene and mitochondrial cox1 gene sequences isolated from Korean Compsopogon caeruleus showed high sequences similarity with Compsopogon caeruleus from worldwide (98.6-100% (18S rDNA), 99-100% (rbcL) and 97.7-100% (cox1)). These sequences similarities support the identification of a red alga found in Korea as Compsopogon caeruleus. This new geographical report provides the useful information for understanding the distribution and habitat range of Compsopogon caeruleus especially concerning urban freshwater environments.

• 한국패류학회지
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• 제31권2호
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• pp.159-163
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• 2015

We report a new record of Myurella nebulosa (Sowerby, 1825) collected by SCUBA diving from sandy bottom of subtidal zone in Jeju Island in Korea. The specimen of M. nebulosa is distinguished from its congeners by having slightly plump shell, high spire, distinctive subsutural band, deep axial ribs, numerous and shallow spiral ribs, ivory in color. In this report, we provided a brief morphological description and photographs of M. nebulosa. An unrooted phylogenetic tree for mitochondrial cox1 sequences using NJ analysis found Korean M. nebulosa sample was clustered with its conspecific sequences, but clearly separated from M. columellaris that is not easy to distinguishable based on external shell morphology alone.

• Parasites, Hosts and Diseases
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• 제54권6호
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• pp.813-817
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• 2016

Armillifer agkistrodontis (Ichthyostraca: Pantastomida) is a parasitic pathogen, only reported in China, which can cause a zoonotic disease, pentastomiasis. A complete mitochondrial (mt) genome was 16,521 bp comprising 13 protein-coding genes (PCGs), 22 tRNA genes, 2 rRNA genes, and 1 non-coding region (NCR). A phylogenetic tree drawn with the concatenated amino acid sequences of the 6 conserved PCGs (atp6, cox1-3, and nad2) showed that A. agkistrodontis and Armillifer armillatus constituted a clade Pentastomida which was a sister group of the Branchiura. The complete mt genome sequence of A. agkistrodontis provides important genetic markers for both phylogenetic and epidemiological studies of pentastomids.

• 한국어류학회지
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• 제33권4호
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• pp.217-225
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• 2021

양태과는 경제적으로 중요한 저서성 바닷물고기로써 인도태평양과 지중해의 열대 또는 온대지역의 하구역에 서식한다. 이번 연구에서 우리는 차세대염기서열분석법을 이용하여 flathead의 일종인 Platycephalus cultellatus Richardson, 1846의 전장 미토콘드리아 유전체를 최초로 분석하였다. 그 총 길이는 16,641 bp이었고, 단백질암호화 유전자 13개, 리보솜 RNA 유전자 2개, 전량 RNA 유전자 22개로 구성되었다. 그 유전자의 구성과 배열은 전형적인 척추동물과 같았다. 단백질암호화 유전자 13개를 바탕으로 작성된 분자계통수에서 P. cultellatus는 같은 과에 속하는 종들과 단계통군을 형성하였고, P. indicus를 비롯하여 Platycephalus sp.로 등록된 표본들과 함께 분기하였다. 또한 DNA 바코딩 분자마커로 널리 사용되는 cox1 유전자를 바탕으로 작성된 분자계통수에서 우리의 표본은 같은 종에 속하는 표본들과 단계통군을 형성하여 그 분류학적 위치가 명확하게 밝혀졌다. 이번 연구에서 새롭게 분석된 P. cultellatus의 미토콘드리아 유전체는 이후 flatheads의 분류와 분자계통을 위한 중요한 기초정보로 활용될 것이다.