• Title/Summary/Keyword: mino acid spquence

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Isolation and Characterization of a Cdna ( Fp 1 ) Encoding the Iron Storage Protein in Red Pepper ( Capsicum annuum L. )

  • Kim, Ho-Young;Lee, Young-Ok;Noh, Ill-Sup;Kang, Hee-Wan;Kameya, Toshiaki;Saito, Takashi;Kang, Kwon-Kyoo
    • Plant Resources
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    • v.1 no.1
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    • pp.13-21
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    • 1998
  • A cDNA Fragment encoding iron storage protrin generated by polymerase chain reaction(PCR) using highly conserved regions of ferritin related genes were used to sereen a red pepper cDNA library. cDNA clone was designated as Fp1. Fp1 clone contatines a 5' nontranslated region of 51dp containing stop conds. Down stream from 5' UTP. an open reading frame of 750bp was observed. followed by a 3' UTR of 272bp. The deduces amino acid sequence of red pepper protein(Fp1) showed 84%, 48% and 36% identity with soybean(SolC). human(HuL H) and horse spleen(HoS-L) ferritin mRNA accumulation in response to iron. Ferritin mRNA accumulation was transient and particularly abundant in leaves. reaching a maxmum at 12h. The level of ferritin mRNA in roots was affected to a lesser extent than in leaves.

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