• Title/Summary/Keyword: miniature society

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Reproductive Characteristic of Transgenic Massachusetts General Hospital Miniature Pigs for Xenotransplantation (Massachusetts General Hospital 미니돼지 유래 이종이식용 형질전환 돼지의 번식 특성 분석)

  • Ji, Soo-Jeong;Lee, Gunsup;Park, Sang Hyoun;Kim, Kyung Woon;Byun, Sung-June;Ock, Sun A;Hwang, Seongsoo;Woo, Jae-Seok;Oh, Keon Bong
    • Journal of Embryo Transfer
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    • v.32 no.3
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    • pp.165-170
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    • 2017
  • Pigs have been extensively used as mediators of xenotransplantation research. Specifically, the Massachusetts General Hospital (MGH) miniature pig was developed to fix major histocompatibility antigens for use in xenotransplantation studies. We generated transgenic pigs for xenotransplantation using MGH pigs. However, it has not been studied yet whether these pigs show similarity of reproductive physiological characteristics to wild types of MGH miniature pig. In this study we analyzed the estrous cycles and pregnancy characteristics of wild type (WT) and transgenic MGH miniature pigs, which were ${\alpha}1,3$-galactosyltransferase (GalT) heterozygous and homozygous knock-out, and membrane cofactor protein (MCP) inserted in its locus, $GalT^{-MCP/+}$ and $GalT^{-MCP/-MCP}$ pigs. Estrous cycles of WT, $GalT^{-MCP/+}$ and $GalT^{-MCP/-MCP}$ pigs were $20.9{\pm}0.74$, $20.1{\pm}1.26$, and $17.3{\pm}0.87days$, respectively, and periods of estrous were $3.2{\pm}0.10$, $3.1{\pm}0.12$, and $3.1{\pm}0.11days$. The periods of gestation of WT, $GalT^{-MCP/+}$ and $GalT^{-MCP/-MCP}$ pigs were $114.2{\pm}0.37$, $113.3{\pm}0.67$, and $115.4{\pm}0.51days$, respectively. Litter sizes of WT, $GalT^{-MCP/+}$ and $GalT^{-MCP/-MCP}$ pigs were $4.8{\pm}0.35$, $4.8{\pm}1.11$ and $3.0{\pm}0.32$ respectively. There were no significant differences on estrous cycle, periods of estrous and gestation, and litter size among WT, $GalT^{-MCP/+}$ and $GalT^{-MCP/-MCP}$ pigs, meaning that GalT knock-out and additional expression MCP of the MGH miniature pig did not effect on reproduction traits. These results provide relevant information to establish breeding system for MGH transgenic pig, and for propagation of $GalT^{-MCP/-MCP}$ pig to supply for xenotransplantation research.

Development of a New Precision Actuator by Bi-morph Type PZT to Realize Nano/Micro Mechanical Testing in MUTM (바이몰프형 PZT를 이용한 소형만능재료시험기용 정밀 구동 액추에이터의 개발)

  • Kweon, Hyun-Kyu;Choi, Seong-Dae;Cheong, Seon-Hwan
    • Journal of the Korean Society of Manufacturing Process Engineers
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    • v.5 no.1
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    • pp.45-50
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    • 2006
  • This paper shows a new precision actuator of MUTM(miniature universal testing machine) for the testing of compression and tensile load on the MEMS materials and structures. The MUTM consists of a sample holder, an ultraprecision precision actuator(tranlation stage) and load sensor. The precision actuator has been developed for generating displacements with nanometer accuracy and a dynamic range of 1mm simultaneously. In this paper, it can be made by using the displacement property of bi-morph type PZT, which is able to extend the long range(stroke) according to cantilever size. However, it is not enough to be generated for compression and tensile load in miniature universal testing machine. Therefore, three dozen bi-morph type PZTs are used for generating the load. The load and displacement of the precision actuator are 35g and 0.4mm respectively.

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A Study on the Go-go-kwan(姑姑冠) -With a Special Reference to "Jip-sa(集史)"- (고고관(姑姑冠)에 관한 연구 - "집사(集史)"를 중심으로 -)

  • Kim, Mi-Ja
    • Journal of the Korean Society of Costume
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    • v.58 no.3
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    • pp.105-115
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    • 2008
  • A Go-go-kwan is a particular hat which has worn by married woman in Mongol and Yuan dynasty in China. It is also widely called as a Boktak in Mongol. The Go-go-kwan, recorded in jip-sa and in the miniature painting of 14th century is the early type of go-go-kwan. Later type can be seen from the relic collections of Gyeonggi Provincial Museum in Korea. The Go-go-kwan is composed with three parts ; feather part, tube part and hat part. Among the feathers, the largest one is as long as four times of the length of the tube and the shortest one is half the length of the tube. The tube is made of light weight wood such as white birch or bamboo, and covered with silk. Some of the hats were made exactly to fit on the head whereas others were designed to wear on the head as like jobawi.

A Study on the Woon Kyun of Won - Focused on the Jipsa - (원의 운견에 관한 연구 -집사를 중심으로-)

  • 김미자
    • Journal of the Korean Society of Costume
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    • v.53 no.2
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    • pp.127-135
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    • 2003
  • Woon Kyun was one of the shoulder cloth worn by both men and women during Won(원) Dynasty. The distinet features of Woon Kyun was that it had cloud-form embroidered on chest, back and both shoulders respectively and the front of it was opened. It also glitterinyly decorated with five different colored embroidery inserted with gold thread. 26 Woon Kyun out of 59 men demonstrated on the miniature of ≪Jipsa≫ were selected and analyzed according to the historical periods, class and sex. The results of the study showed that there was little change on its forms and patterns and people wore it without specific differentiation. The common patterns of embroidery used were various kinds of animals and plants, however, plants seemed to be more preferred.