• Title/Summary/Keyword: milt

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The analysis of heavy metal total contents In milt of Han river (한강유역 저니층의 중금속 함량 전분석)

  • 정일현;김세진
    • Journal of environmental and Sanitary engineering
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    • v.9 no.2
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    • pp.63-71
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    • 1994
  • An analytic and comparative study on the level of concentration on heavy metals in silt of Han- River was described. The Paldang lake supplies tap water resources for the 20 million residents in the metropolitan zone. It is composed that three steps for water works by the water volume in all 35,072 thousand tons : the first section is 7,251 thousand tons, the second is 15,980 thousand tons and the third is 11,841 thousand tons. However, recently it has becoming a serious social issue for water contamination on Paldang by heavy metals due to gathering of silt. It has concentrated on analysis and comparison study on the level of heavy metals between contamination- estimated zone and the other zones. After all, Concentration on heavy metals of expected none pollute's zone and expected pollute's zone indicate almost similar results. In the end, heavy metals within silt of Han- River consider not to effect on pollution but quantity of natural condition.

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Effect of Diluents and Temperature on Sperm Storage in Starry Flounder (Platichthys stellatus) (희석액과 보존온도에 따른 강도다리 (Platichthys stellatus) 정자의 냉장보존 효과)

  • Lim, Han-Kyu;An, Cheul-Min;Son, Maeng-Hyun;Park, Min-Woo;Kim, Eung-Oh;Byun, Soon-Gyu
    • Journal of Aquaculture
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    • v.19 no.1
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    • pp.47-51
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    • 2006
  • Experiments were performed to find out the physico-chemical properties of milt and sperm motility in various storage conditions using starry flounder (Platichthys stellatus). The average sperm concentration and spermatocrit in stripped milt were $6.00{\pm}0.98{\times}10^8/mL\;and\;72{\pm}5$, respectively. The osmolality and pH were $337{\pm}9mmol/kg$ and $7.7{\pm}0.1$, respectively. The sperm of starry flounder was preserved with artificial seminal plasma (ASP), Stein's solution (SS) and marine fish Ringer's solution (MFRS) at $0^{\circ}C,\;2^{\circ}C,\;and\;4^{\circ}C$. The most effective condition for cold storage was SS at $0^{\circ}C$, and the preserved sperm remained motile for 30 days.

Evaluation of Fertility of Artificial Induced Gynogenetic Diploid Male in Paralichthys olivaceus (유도된 자성발생성 2배체 숫컷 넙치(Parlichthys olivaceus)의 생식 능력 평가)

  • KIM Bong-Seok;MOON Young Bong;JEONG Chang Hwa;KIM Dong Soo;LEE Young-Don
    • Journal of Aquaculture
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    • v.7 no.3
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    • pp.151-158
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    • 1994
  • To evaluate the reproductive ability of gynogenetic diploid male. Paralichthys olivaceus. histological analysis of testis. cytological analysis of spermatozoa and fertilization test with normal aggs were studied and the results are as follow; The gonads of gynogenetic diploid male were histologically normal. and many spermatozoa were observed in their testis. Number of spermatozoa from the control and gynogenetic diploid male were $2.58\times10^9$ and $2.42\times10^9$ cells per 1 ml of milt. respectively (P> 0.05). Amount of milt per kg body weight from the gynogenetic diploid male was significantly higher (P< 0.01) than that from the control male (8.3ml). Size and morphology from the two experimental groups were not different (P>0.05). More than $80\%$ of fertilization rates and hatching rates were observed when the eggs from the control were fertilized with the gynogenetic diploid male sperms.

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Effect of Diluents for Cold Storage of Olive Flounder (Paralichthys olivaceus) Sperm (희석액벼려 넙치(Paralichthys olivaceus) 정자의 냉장보존 효과)

  • LIM Han Kyu;AN Cheul Min;SON Maeng Hyun;PARK Min-Woo;PARK Yun Jung
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.38 no.4
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    • pp.239-244
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    • 2005
  • The effects of diluents composition on cold storage for olive flounder (Paralichthys olivaceus) sperm were examined in terms of the swimming speed of sperm, the percentage of mobile sperm, and fertilization rates. The following results indicated that cold storage methods with fresh conditions could be employed in olive flounder milt preservation. The preserved sperm of olive flounder that was diluted I: 10 with artificial seminal plasma II (ASP II) and Stein's solution (SS) at $0^{\circ}C$ remained motile for 30 days. The most effective condition for cold storage was ASP II and SS at 0 $0^{\circ}C$ for the sperm, although there is no significant difference statistically. No difference in the fertilization rate was found between fresh sperm and the preserved ones with ASP I, II and SS at 10 days post-storage.

Diluents and Cryoprotectants for Cryopreservation of Filefish Thamnaconus modestus Sperm

  • Le, Minh-Hoang;Lim, Han-Kyu;Min, Byung-Hwa;Son, Maeng-Hyun;Lee, Jung-Uie;Chang, Young-Jin
    • Journal of Aquaculture
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    • v.21 no.1
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    • pp.54-59
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    • 2008
  • The present study aimed to find the best diluent and cryoprotectant for sperm cryopreservation of filefish Thamnaconus modestus. Two kinds of artificial seminal plasma(ASP1, ASP2), 0.3 M glucose and marine fish Ringer's solution(MFRS) were employed as diluent. Dimethyl sulfoxide(DMSO) and methanol as cryoprotectant were selected for sperm cryopreservation. Sperm was diluted at the ratio of 1:3 with diluents containing cryoprotectants and adjusted for final concentration at 5%, 10%, 15% and 20%. Mixed milt was frozen at liquid nitrogen vapor after equilibration for 5 min. The highest motility($40.5{\pm}2.8%$) and swimming speed($81.5{\pm}4.1{\mu}m/s$) of frozen/thawed sperm were observed in ASP1 diluent containing 10% DMSO and in ASP2 containing 15% DMSO, respectively. Results showed that cryopreservation with ASP as diluent and DMSO as cryoprotectant could be adopted for long term storage of filefish sperm.

The Lateral Load Capacity of Bored-Precast Pile Depending on Injecting Ratio of Cement Milk in Sand (사질토 지반에서 시멘트밀크 주입비에 따른 매입말뚝의 수평지지력)

  • Hong, Won-Pyo;Yun, Jung-Mann
    • Journal of the Korean Geosynthetics Society
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    • v.12 no.4
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    • pp.99-107
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    • 2013
  • In order to investigation Lateral bearing capacity of bored-precast pile, we carried out the analysis of the relationship between Lateral load and horizontal displacement using the result of horizontal pile load test. The six piles injected cement milk of 50%, 70% and 100% of the embedded length of pile were used in the horizontal pile load test. The horizontal displacement, yielding load and horizontal bearing capacity are mainly affected by The injecting ratio of cement milk (injected length of cement milk/embedded length of pile). As the injecting ratio of cement milt is increased, the starting point of horizontal displacement in piles become close to the ground surface and the amount of horizontal displacement is decreased. Also, the horizontal bearing capacity and yielding load are highly increased with increasing the ration of cement milk. The horizontal bearing capacity and yielding load of bored pile with 1 of cement milk ratio are about two or three times those of pile with 0.5 of cement milk ratio.

Food Preferences and Dietary Habits of University Students in Kyungbuk Province (경북 일부 지역 남, 녀 대학생들의 식품기호도와 식습관에 관한 연구)

  • 박경애
    • Journal of the East Asian Society of Dietary Life
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    • v.13 no.6
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    • pp.527-541
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    • 2003
  • The purpose of this study was to investigate food preferences and dietary habits of university students in Kyungbuk province. We assessed anthropometric measurements, food preferences, and dietary habits of seventy nine male and sixty nine female students at K university. The results of this study were analyzed with $\chi$$^2$, t, and ANOVA test using SPSS package program. The percentages of students who were underweight, normal and overweight by body mass index (BMI) were 18.0, 22.1, and 27.7 for males, and 17.8, 21.7, and 28.7 for females, respectively. Preferences for noodles with bean sauce, bread (castella, red-bean bread), beef, pork, chicken, ham, garlic, milk soy bean milt carbonated drink ionic beverages, bun (fried, steamed), croquette, soju(alcohol) and tobacco were higher in males than females, but preferences for tangle, strawberry and orange were lower in males than females. Males did not skip meals compared to females, and ate more meat fat than females. Males exercised more frequently and longer than females. Eighty six point eight percent of females and 69.7% of males were dissatisfied with their weights, and females attempted the weight control more than males. Preferences for bibimbab(boiled rice with assorted mixtures), ionic beverages, and beer were shown higher in overweight males than in normal ones and preference for red fish, hamburger, and chocolate were higher in underweight females than overweight ones. Overweight females skipped meals more and ate more meat fats than normal weight ones. Normal weight males and underweight females were satisfied with their weight. The results of this study suggest that university students need more nutritional education, and modification of dietary habits based on food preference to prevent and treat obesity and to maintain their health.

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Effect of Diluents on the Short-Term Storage of Sperm in Black Seabream, Acanthopagrus schlegeli (희석액별 감성돔, Acanthopagrus schlegeli 정자의 단기보존 효과)

  • LIM Han Kyu;KHO Kang Hee;CHANG Young Jin
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.30 no.2
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    • pp.211-215
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    • 1997
  • Experiments were performed to find out the proper conditions of diluents for the short-tenn storage of sperm of black seabream, Acanthopagrus schlegeli. For milt collection, brood stock was reared in a recirculating seawater system. The following results indicated that short-term storage methods with fresh condition could tie employed in black seabream sperm. When the black seabream's serum was used as a diluent for the fresh storage, sperm activity index (SAI), fertilization rate and survival rate derived the best result in $7\~10\;days$ of storage. pH 7 and 8 showed the highest SAI in the same storage condition. In order to keep high SAI and survival rate of the sperm, addition of 800 ppm neomycine into the diluent revealed the best storage results.

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Effects of Diluents and Cryoprotectants on Sperm Cryopreservation in Starry Flounder (Platichthys stellatus) (강도다리(Platichthys stellatus) 정자의 냉동보존에 미치는 희석액과 동해방지제의 영향)

  • Lim, Han-Kyu;An, Cheul-Min;Noh, Gyong-Ane;Min, Byung-Hwa
    • Journal of Aquaculture
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    • v.20 no.3
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    • pp.173-177
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    • 2007
  • An experiment was performed to obtain cryopreservation techniques of starry flounder (Platichthys stellatus) sperm. Milt obtained from 24 males were cryopreserved using two diluents, artificial seminal plasma (ASP) and Stein's solution (SS) with three cryoprotectants, dimethyl sulfoxide (DMSO), methanol, and glycerol concentrated from 5% to 20%. Post-thaw sperm activity (motility and/or speed) revealed the highest in 10% DMSO and 15% methanol in ASP and SS as diluent. Motility and speed of cryopreserved sperm were decreased according to increase of glycerol concentration. To conclude, DMSO was a better cryoprotectant than methanol or glycerol for cryopreservation of starry flounder sperm. Glycerol was incongruent cryoprotectant because of toxic to starry flounder sperm. Most cryopreserved spermatozoa without cryoprotectant showed the enlarged head with granulated chromatin and ruptured plasma membrane by freezing and thawing injuries compared with unfrozen normal spermatozoa.

Study on the Antioxidant Activity of Soybean Products Extracts for Application of Animal Products (축산제품에 응용하기 위한 콩제품 추출물의 항산화 능력에 관한 연구)

  • Lee Chi-Ho;Moon Seong-Yong;Lee Jung-Chil;Lee Ji-young
    • Food Science of Animal Resources
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    • v.24 no.4
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    • pp.405-410
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    • 2004
  • This study was performed to investigate the antioxidative activity of soybean products extracts using 1, 1-diphenyl-2-picrylhydrazyl (DPPH) and thiobarbituric acid (TBA) method for application of animal products. All three extracts (natto, soybean paste, soybean milk extract) were found to have an ability to donate hydrogen to DPPH. Especially, natto and soybean paste extract were more effective than that of soybean milk on the antioxidative activities. Natto and soybean paste extract were stronger than butylhydroxy toluene (BHT), butyl hydroxyl anisole (BHA) (5${\times}$10$^{-4}$ M) and ascorbic acid (5.7${\times}$10$^{-3}$ M) previously well known as antioxidants. These extracts also showed a synergistic effect. TBA values of natto (45.8%) and soybean paste extract (45.2%) were stronger than that of soybean milk extract (33.6%). These results suggest that soybean product extracts have antioxidative activities and synergistic effects.