• 한국콘크리트학회:학술대회논문집
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• 한국콘크리트학회 2001년도 가을 학술발표회 논문집
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• pp.793-796
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• 2001

Physical properties of concrete, Such as, compressive strength, permeable pore and penetration depth of chloride ion were investigated. And to investigate the effect of containing GGBF in concrete, the diffusion coefficient of chloride was measured through an electro- migration test. The diffusion coefficient of chloride was decreased with increase of replacement ratios of GGBF when compared to OPC. Relation coefficients between physical properties of concrete and diffusion coefficient of chloride were more than 0.9.

• 한국건축시공학회:학술대회논문집
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• 한국건축시공학회 2008년도 추계 학술논문 발표대회
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• pp.219-226
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• 2008

• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 2000년도 춘계학술대회논문집
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• pp.1889-1893
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• 2000

During the test drive of developing vehicle, a low frequency vibration of sprung mass on front outer wheel has been frequently observed in cornering with some speed. The purpose of this paper is to investigate the low frequency vibration of the sprung mass. The analysis result shows that the low frequency vibration is caused by sudden migration of the center of gravity of vihicle and it is determined by geometric points of suspension.

• Asian Pacific Journal of Cancer Prevention
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• 제15권20호
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• pp.8685-8688
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• 2014

The objective of the present study was to investigate cloning, expression, and functions of the recombinant protein, Siva1. Siva1 gene was synthesized by RT-PCR from HCT116 cells. Plasmids were cleaved with the restriction endonuclease, BamH1/Sal1 and products were connected to pQE30, which underwent cleavage by BamH1/Sal1. The recombinant plasmid, pQE30-Siva1, was identified after digestion with restriction endonucleases followed by transformation into E. coli M15. Expression of Siva1 was induced by IPTG and identified by SDS-PAGE following purification with affinity chromatography. The results showed that size of Siva1 was 12 kDa, consistent with the molecular weight of the His-Siva1 fusion protein. Functional test demonstrated that Siva1 significantly inhibited the invasion and migration of HCT116 cells. It may thus find clinical application for control of cancers.

• Fisheries and Aquatic Sciences
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• 제16권4호
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• pp.243-251
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• 2013

Genetic diversity and gene flow patterns in Pollicipes mitella were investigated with a nucleotide sequence analysis of 514 base pairs from the mitochondrial cytochrome c oxidase subunit I gene (COI) in 124 samples collected from six Korean populations. In total, 59 haplotypes were defined by 40 variable nucleotide sites in the COI region. The haplotypes had shallow haplotype genealogy and no geographic associations. All populations had high haplotype diversity (0.909 to 0.979) and low nucleotide diversity (0.0055 to 0.0098). The haplotypes with recently diverged nucleotides were distributed by long-range larvae dispersal among regional populations. The pairwise fixation indices ($F_{ST}$) estimated with the exact test and migration rates indicate that substantial gene flow has occurred among populations as a result of sea currents, except between the Uljin (East Sea coast) and other Korean populations. This suggests that significant genetic differentiation and low migration rates have affected the Uljin population.

• Archives of Plastic Surgery
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• 제37권1호
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• pp.7-11
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• 2010

Purpose: Vascular endothelial growth factor (VEGF) is known as a growth factor of endothelium and fibroblast. The purpose is to know the VEGF effects on fibroblast proliferation and fibroblast's notch receptor expression. Methods: CCD-986sk fibroblast was purchased from the Korean Cell Bank and was used in XTT assay for proliferation and wound healing assay for migration. Immunofluorescent (IF) staining and western blotting were used in testing notch expression of fibroblast. Semiquantitative RT-PCR was used in checking notch 1 mRNA production by fibroblast. Student-t test was used for analyzing results. Results: Cell proliferation assay using XTT showed significant higher proliferation in VEGF treated fibroblast, $2.324{\pm}0.0026$ vs. $2.463{\pm}0.017$ (p=0.002). Wound healing assay showed longer migration in VEGF treated fibroblast (p=0.062). The fluorescence was brighter in VEGF treated cells of notch 1 IF staining. Notch 1 expressions and mRNA productions increased more in VEGF treated cells. Conclusion: VEGF stimulates fibroblast to proliferate, migrate and to express Notch 1 simultaneously. Notch receptor could be related to VEGF mediated wound healing.

• Animal cells and systems
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• 제15권3호
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• pp.243-249
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• 2011

The genetic diversity and population history of the Asian shore crab, Hemigrapsus sanguineus, were investigated with a nucleotide sequence analysis of 536 base pairs (bp) of the mitochondrial cytochrome c oxidase subunit I gene (COI) in 111 samples collected from four populations in Korea and one in Japan. In total, 28 haplotypes were defined by 27 variable nucleotide sites in the COI region examined. The observed haplotypes had a shallow haplotype genealogy and no geographical associations. Most of the populations had high haplotype diversity (0.656-0.788) and low nucleotide diversity (0.00165-0.00244), and significant negative values for Fu's $F_S$, suggesting rapid and recent population growth from an ancestral population and sudden population expansion. The pairwise fixation indices ($F_{ST}$) estimated with the exact test and the migration rates indicate that substantial gene flow occurs among these populations as a result of sea currents, except between the Yellow Sea coast of Korea (BUA) and the Pacific Ocean coast of Japan (JPA). These two populations (BUA and JPA) showed significant genetic differentiation and low migration rate.

• 한국미생물·생명공학회지
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• 제48권4호
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• pp.569-573
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• 2020

Brucellosis is a zoonotic disease caused by Brucella infections and humans usually contract this disease from close contact with infected animals or their products, usually via the ingestion of cheese or crude milk. Macrophage migration inhibitory factor (MIF) and Pro- and anti-inflammatory cytokines play an important role in susceptibility/resistance and the immunopathogenesis of Brucella infection. These cytokines are crucial factors in the initiation and progression of protective immunity against Brucella infection but the role of MIF has not been well studied in the human response to intracellular microbes. This study was designed to investigate the effect of MIF expression on Brucella susceptibility. A total of 85 positive rose Bengal tests and 24 samples from healthy individuals were collected for this study and subjected to polymerase chain reaction assays (PCR) of the bcsp31 diagnostic gene. MIF concentrations were evaluated using Enzyme-Linked immunosorbent assay (ELISA) and the results showed that 46 (54%) of the rose Bengal test samples were positive and 39 (46%) were negative for bcsp31 (p ≤ 0.05) and used as the gold standard for all of the comparisons in this study. The ELISA results indicate that the mean concentration of MIF was significantly higher in patients with positive rose Bengal tests when compared to the control groups and that its concentration increases with increasing age in both the patient and control groups (p ≤ 0.05).

• Geomechanics and Engineering
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• 제30권2호
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• pp.201-210
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• 2022

This paper presents a study regarding the influence of various water levels on the characteristics of subgrade mud pumping through a self-developed test instrument. The characteristics of mud pumping are primarily reflected by axial strain, excess pore water pressure, and fine particle migration. The results show that the axial strain increases nonlinearly with an increase in cycles number; however, the increasing rate gradually decreases, thus, an empirical model for calculating the axial strain of the samples is presented. The excess pore water pressure increases rapidly first and then decreases slowly with an increase in cycles number. Furthermore, the dynamic stress within the soil first rapidly decreases and then eventually slows. The results indicate that the axial strain, excess pore water pressure, and the height and weight of the migrated fine particles decrease significantly with a low water level. In this study, when the water level is 50 mm lower than the subgrade soil surface, the issue of subgrade mud pumping no longer exist.

• 마이크로전자및패키징학회지
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• 제17권4호
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• pp.11-17
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• 2010

차세대 패키징 기술을 실현하기 위해서는 극복해야 할 기술적인 과제들이 많이 존재한다. 그 중에서 솔더 접합부의 EM에 의한 고장은 오랜전부터 알려진 과제이지만, 고밀도화, 파인핏치화, 발열문제가 심각해지면서 현실적인 문제로 인식되기 시작했다. 더욱이 솔더가 무연화 되면서 다양해진 구성원소들의 영향에 대한 연구가 시작되었다. 지금까지의 연구결과를 종합해보면 Sn-Pb 공정솔더 보다 무연솔더는 EM에 대한 저항성이 강한 것으로 보여진다. 하지만, 무연솔더 접합부에서 발생하는 EM현상에 대해서는 아직 밝혀지지 않은 부분이 많다. 보이드의 핵 생성 및 성장속도와 전기저항의 급격한 변화와의 관계, Sn 결정립의 방향과 전류밀도에 따른 마이그레이션 속도계수와 수명예측기술, 각종 무연솔더와 시험조건에서의 언더필의 효과 등에 관한 다양한 연구가 필요하다. 또한 무연 플립칩 패키지의 총체적인 신뢰성 확보를 위해, EM과 반도체칩 내부배선의 발열에 기인하는 Thermomigraton, 응력에 기인하는 Stress-migration과의 상관관계에 대한 연구도 요구된다.