• Asian Pacific Journal of Cancer Prevention
• /
• v.15 no.1
• /
• pp.139-143
• /
• 2014

Although correlations between platelets and lung cancer has been recognized, effects on non-small cell lung cancer (NSCLC) metastasis remain to be determined in detail. In the present study, wound healing assays revealed a role of platelets in NSCLC cell migration. Thus the mean migration rate of lung adenocarcinoma A549 cells was significantly elevated after co-culture with platelets ($81.7{\pm}0.45%$ vs $41.0{\pm}3.50%$, P<0.01). Expression of GAPDH was examined by reverse transcription-polymerase chain reaction to study the effect of platelets on NSCLC cell proliferation. The result showed that the proliferation of A549 and SPC-A1 cells was not affected. Mouse models were established by transfusing A549 cells and SPC-A1 cells into mice lateral tail veins. We found tumor metastasis nodules in lungs to be increased significantly after co-transfusion with platelets (in A549, $4.33{\pm}0.33$ vs $0.33{\pm}0.33$, P=0.01; in SPC-A1, $2.67{\pm}0.33$ vs $0.00{\pm}0.00$, P=0.01). In addition, consecutive inoperable patients with newly diagnosed NSCLC (TNM stage III or IV) between January 2009 and December 2011 were retrospectively reviewed. Using the Kaplan-Meier method, NSCLC patients with a high platelet counts demonstrated a significantly shorter progression free survival compared with those with a low platelet count (> $200{\times}10^9/L$, 3 months versus ${\leq}200{\times}10^9/L$, 5 months, P=0.001). An elevated platelet count was also identified as an independent prognostic factor by Cox regression analysis for prgression free survival (adjusted hazard ratio: 1.69; 95% CI: 1.16, 2.46; P=0.006). This study suggested that platelets might contribute to the hematogenous metastatic process by promoting cancer cell migration, which eventually affects the prognosis of NSCLC.

• Journal of Dental Anesthesia and Pain Medicine
• /
• v.18 no.1
• /
• pp.9-17
• /
• 2018

Background: The structure and function of bone tissue is maintained through a constant remodeling process, which is maintained by the balance between osteoblasts and osteoclasts. The failure of bone remodeling can lead to pathological conditions of bone structure and function. Remifentanil is currently used as a narcotic analgesic agent in general anesthesia and sedation. However, the effect of remifentanil on osteoclasts has not been studied. Therefore, we investigated the effect of remifentanil on pre-osteoclast (pre-OCs) differentiation and the mechanism of osteoclast differentiation in the absence of specific stimulus. Methods: Pre-OCs were obtained by culturing bone marrow-derived macrophages (BMMs) in osteoclastogenic medium for 2 days and then treated with various concentration of remifentanil. The mRNA expression of NFATc1 and c-fos was examined by using real-time PCR. We also examined the effect of remifentanil on the osteoclast-specific genes TRAP, cathepsin K, calcitonin receptor, and DC-STAMP. Finally, we examined the influence of remifentanil on the migration of pre-OCs by using the Boyden chamber assay. Results: Remifentanil increased pre-OC differentiation and osteoclast size, but did not affect the mRNA expression of NFATc1 and c-fos or significantly affect the expression of TRAP, cathepsin K, calcitonin receptor, and DC-STAMP. However, remifentanil increased the migration of pre-OCs. Conclusions: This study suggested that remifentanil promotes the differentiation of pre-OCs and induces maturation, such as increasing osteoclast size. In addition, the increase in osteoclast size was mediated by the enhancement of pre-OC migration and cell fusion.

• Tunnel and Underground Space
• /
• v.34 no.2
• /
• pp.89-103
• /
• 2024

In the disposal environment, gases can be generated at the interface between canister and buffer due to various factors such as anaerobic corrosion, radiolysis, and microbial degradation. If the gas generation rate exceeds the diffusion rate, the gas within the buffer may compress, resulting in physical damage to the buffer due to the increased pore pressure. In particular, the rapid movement of gases, known as gas breakthroughs, through the dilatancy pathway formed during this process may lead to releasing radionuclide. Therefore, understanding these gas generation and movement mechanism is essential for the safety assessment of the disposal systems. In this study, an experimental apparatus for investigating gas migration within buffer was constructed based on a literature review. Subsequently, a gas injection experiment was conducted on a compacted bentonite block made of Bentonile WRK (Clariant Ltd.) powder. The results clearly demonstrated a sharp increase in stress and pressure typically observed at the onset of gas breakthrough within the buffer. Additionally, the range of stresses induced by the swelling phenomenon of the buffer, was 4.7 to 9.1 MPa. The apparent gas entry pressure was determined to be approximately 7.8 MPa. The equipment established in this study is expected to be utilized for various experiments aimed at building a database on the initial properties of buffer and the conditions during gas injection, contributing to understanding the gas migration phenomena.

• Korean Membrane Journal
• /
• v.9 no.1
• /
• pp.18-26
• /
• 2007

The transport and removal characteristics of cobalt ions were investigated in a continuous electrodeionization with cation exchange textile (CEDI-CET). It was shown that the removal properties of cobalt ions are strongly dependent on the operating parameters such as temperature, flow velocity, and the solution pH. The transport and removal of cobalt ions was mainly related to the sorption on the surface and the convection and electro-migration through the ion exchange medium. In this study, the CEDI-CET showed good process performance for the removal of metal ions compared with the conventional CEDI with resins.

• ETRI Journal
• /
• v.32 no.6
• /
• pp.932-939
• /
• 2010

In this paper, a fast migration method is proposed. Our method executes local relocation on a model placement where an additional module is added to it for modification with a minimum number of displacements. This method is based on mean-field annealing (MFA), which produces a solution as reliable as a previously used method called simulated annealing. The proposed method requires substantially less time and hardware, and it is less sensitive to the initial and final temperatures. In addition, the solution runtime is mostly independent of the size and complexity of the input model placement. Our proposed MFA algorithm is optimized by enabling module rotation inside an energy function called permissible distances preservation energy. This, in turn, allows more options in moving the engaged modules. Finally, a three-phase cooling process governs the convergence of problem variables called neurons or spins.

• Animal cells and systems
• /
• v.12 no.1
• /
• pp.1-9
• /
• 2008

In the developing limb, chondrogenesis is an important prerequisite for the formation of cartilage whose template is required for bone formation. Chondrogenesis is a tightly regulated multi-step process, including mesenchymal cell recruitment/migration, prechondrogenic condensation of the mesenchymal cells, commitment to the chondrogenic lineage, and differentiation into chondrocytes. This process is controlled exquisitely by cellular interactions with the surrounding matrix and regulating factors that initiate or suppress cellular signaling pathways and transcription of specific genes in a temporal-spatial manner. Understanding the cellular and molecular mechanisms of chondrogenesis is important not only in the context of establishing basic principle of developmental biology but also in providing research direction toward preventive and/or regenerative medicine. Here, I will overview the current understanding of cellular and molecular mechanisms contributing to prechondrogenic condensation processes, the crucial steps for chondrogenesis, focusing on cell-cell and cell-matrix interactions.

• Journal of the Korean housing association
• /
• v.15 no.6
• /
• pp.47-56
• /
• 2004

In the North-eastern districts of China, Northern races inherited and developed their own cultural traditions. The housing of northeastern China has been changed under the influence of environment. Some tribes' housings has been developed, keeping their own peculiar style, while others have divested of their ethnic flavor. To understand these aspects of development, this study focused on the housings in Heilongjiang and Nei Monggol. As for the procedure, this study referred to the literature and carried out a field trip at the same time. The housing of northeastern China has undergone the process to fix their residence after the life of migration in common. In this process, northeastern hosing has changed variously and can be grouped into the several types by shape and material. From the point of view about living pattern, some important living features are extracted by the types.

• Proceedings of the KSME Conference
• /
• 2008.11a
• /
• pp.1455-1457
• /
• 2008

Cell adhesion is a coordinated process involving initial binding of integrin receptors to extracellular matrix (ECM), recruitment of adhesion proteins, and focal adhesion assembly. The formation of mechanically stable focal adhesion assembly of cells within surrounding ECM is a key parameter to direct numerous cellular functions including cell migration, differentiation, and apotosis. With current cell adhesion assays, it is difficult to understand contributions of each coordinated event on evolution of cell adhesion strengthening since cells spontaneously spread upon their adhesion to the substrate, thus remodeling their cytoskeletal structure. In this presentation, novel approaches for analysis of cell adhesion strengthening process based on the combination of mechanical device, micro-patterned substrates, and molecular biological techniques will be discussed.

• Clinical and Experimental Reproductive Medicine
• /
• v.38 no.1
• /
• pp.1-5
• /
• 2011

The maturation process of mammalian oocytes accompanies an extensive rearrangement of the cytoskeleton and associated proteins. As this process requires a delicate interplay between the cytoskeleton and its regulators, it is often targeted by various external and internal adversaries that affect the congression and/or segregation of chromosomes. Asymmetric cell division in oocytes also requires specific regulators of the cytoskeleton, including formin-2 and small GTPases. Recent literature providing clues regarding how actin filaments and microtubules interact during spindle migration in mouse oocytes are highlighted in this review.

• Journal of Korean Society of Rural Planning
• /
• v.4 no.2
• /
• pp.58-69
• /
• 1998

This paper is to describe the characteristics of the Small Towns development in Rural China. The policies to develop Small Towns have been induced for solving the problem raised during the rural reform. The changes and adaptations of rural reform processes are integrated in Small Towns. The characteristics in the process of development of Ronggang, a small town, are as follows. First, it was the fund raised by rural inhabitants that was used for construction and development of the small town, Second, by implementing the policies of selective migration, the inhabitants of the small town have been differentiated from those of rural region. Third, the activities of commerce and industry of Ronggang have progressed to joint-stock system. Fourth, Having the spatial characteristics of self- development, the small town has grown and is expected to grow higher hierarchy of settlement system.