• Microbiology and Biotechnology Letters
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• v.27 no.5
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• pp.415-418
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• 1999

Thecodiplosis japonensis is an important insect pest in pine trees and hard to be controlled by chemical pesticides due to its characteristic life cycle. Experiments were conducted to determine the pathogenicity and growth temperature of several entomopathogenic fungi as potential microbial insectcides for T. japonensis. Beauveria bassiana SFB-SC and Paecilomyces fumosoroseus SFP-198 were highly pathogenic against T. japonensis. B. bassiana SFB-SC had also pathogenicity against a beneficial insect Bombyx mori. Growth rate and pathogenicity of P. funmosoroseus SFP-198 under 15$^{\circ}C$ was higher than those of B. bassians SFB-SC. These results indicated that P. fumosoroseus SFP-198 is a potential microbial control candidate for control of T. japonensis.

• Korean journal of applied entomology
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• v.43 no.3 s.136
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• pp.185-188
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• 2004

Feltiella acarisuga Vallot, a predatory gall midge preying upon spider mites, is reported for the first time in Korea. It was found in a greenhouse on Glycine soja Sieb. et Zucc. heavily infested with Tetranychus urticae in Sachon, Kyongnam Province. This species is very useful biological control agent for spider mites on greenhouse vegetable crops. Brief morphological characteristics are described.

• Korean journal of applied entomology
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• v.42 no.1
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• pp.77-79
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• 2003

Obolodiplosis robiniae (Haldeman, 1987) (Diptera: Cecidomyiidae), a pest species on a black locust tree, which was originally distributed in North America, is newly recorded from Korea. The specimens were collected from the central Korea. Morphological diagnoses of the both sexes and the symptoms on the host plants are provided.

• Environmental Mutagens and Carcinogens
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• v.25 no.1
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• pp.32-39
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• 2005

In order to identify potential biomarkers of environmental monitoring, we evaluated heat shock genes expressions as effects of various environmental pollutants (nonylphenol, bisphenol-A, 17a­ethynyl estradiol, bis(2-ethylhexyl)phthalate, endosulfan, paraquat dichloride, chloropyriphos, fenitrothion, cadmium chloride, lead nitrate, potassium dichromate, benzo[a]pyrene and carbon tetrachloride) on larvae of aquatic midge Chironomus tentans (Diptera, Chironomidae). Heat shock protein 70 gene expression increased in most of chemicals treated larvae compared to control. The response was rapid and sensitive to low chemical concentrations but not stressor specific. In conjunction with stressor specific biomarkers, heat shock protein 70 gene expression in Chironomus might be developed for assessing exposure to environmental stressors in the fresh water ecosystem. Considering the potential of Chironomus larvae as biomonitoring species, heat shock gene expression has a considerable potential as a sensitive biomarker for environmental monitoring in Chironomus.

• Journal of Korean Society of Forest Science
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• v.50 no.1
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• pp.56-71
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• 1980

To study the changes of vegetational composition of each plant successional stage of Pinus densiflora forest caused by pine gall midge damage, Thecodiplosis japonensis, located in Kyonggi-do area, the middle part of Korea, four districts, Anseong, Yongin, Gwangju, and Gapyeong, were selected according to the length of insect damage duration. The forest in the Anseong district was the noninfested one selected as the check. And forests in Yongin, Gwangju, and Gapyeong were the recent]y damaged, severely damaged, and damage prolonged ones in sequence. It was considered that this order could be in concord with the gradual transition of plant successional stage. In view of temperature, precipitation and soil factors, these four districts were sited within the same environment. Four survey plots were selected from each district and the distance between them was 3~4 km. Then again four subplots were set within a plot. Through needle accumulation after insect infestation, light intensity, soil moisture content, and the amount of soil organic matter were increased and these conditions could create a favorable environment enabling the new species invasion and their growth. These were naturally closely related with the increase in quantity of Pinus densiflora defoliation. After considerable time lapse, the quantities of above mentioned factors decreased with the development of the new substitute tree species. It was found that Quercus mongolica was an excellently substitute tree species come first in this studied area. Species diversity increased to a degree at the early stage of the duration of pine gall midge damage and decreased thereafter. As the same manner, similarity index value between plots in the same district decreased to a degree and increased thereafter. This was linked admittedly with the increase and decrease of tree species and evenness. Synthesizing above mentioned results, the process of plant succession studied quantitatively by changes of relative density and relative importance value, it can be concluded that Pinus densiflora forest gives way to Genus Quercus forest in which Genus Lespedeza and Genus Rhododendron species become as subdominance species after removal of the Pinus densiflora trees damaged by pine gall midge.

• Journal of Korean Society of Forest Science
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• v.65 no.1
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• pp.1-11
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• 1984

Susceptible trees of Pinus densiflora and resistant trees of Pinus rigida following pine gall midge (Tnecodiplosis japonensis Uchida et Inouye) attack were seasonally compared to examine the variation in needle growth and photosynthetic ability, respiration rate, chlorophyll contents, carotenoid and anthocyanin contents. Also, carotenoid and anthocyanin contents of larvae both from soil and from galled tissue were compared during March and September, respectively. The plantation damaged severely by this insect consisted mostly of 10-to 15-year old P. rigida and P. densiflora. The results obtained in this study were as follows: 1) The length of the infested needles of P. densiflora decreased by 48.1 percent compared with the normal needles, while that of P. rigida did 37.4 percent. 2) All of P. densiflora and P. rigida showed higher photosynthetic ability in normal needles than in infested needles. The maximum photosynthetic ability of P. densiflora was shown in mid-August, while that of P. rigida in mid-October. In contrast to that, respiration rate of infested needles was higher than that of normal needles in both species. The respiration rate of P. rigida was higher than that of P. densiflora. 3) P. rigida had higher total chlorophyll contents than P. densiflora. The total carotenoid contents tents in infested needles were higher than those in normal needles of both species. 4) Total carotenoid contents were generally higher in P. rigida than in P. densiflora during the growing season. The total carotenoid content (0.094mg/g) in larvae from soil was similar to that (0.092mg/g) in larvae from galled tissues. 5) Infested needles of both species showed higher anthocyanin contents than normal needles. Higher anthocyanin contents in galled needles were due primarily to its active formation stimulated by larval attack. Thus, reddish-brown coloration occurred only in galled needles of P. densiflora.

• Korean journal of applied entomology
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• v.18 no.4 s.41
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• pp.153-160
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• 1979

The present experiments were carried out to reduce the population density of adult pine gall midge, Thecodiplosis japonensi,s UCHIDA et INOUYE, by means of spray the sticky agent C-4, on the ground, herbacious plantation and foliar leaf of pine trees at Sanbonli,. Anyang, Kyungido. And also the 3 periods of treatment, such as before 2 weeks( I ), before 1 week (II) from the peak emergence period and peak emergence period (III) were applied. The experimental results obtained were as follows. (1) The formula of selected sticky agent C-4 was $70\%$ of castor oil, $25\%$ of damar resin and $5\%$ of carnauba wax, and it showed the best both on stickiness and duration, and lower phytotoxicity to the host plant. (2) The reduction of population densities of the adult PGM were $68\%$ and 78f: in the G-I and G-II treatment plot respectively, which compared to control, on the ground spray. (3) And the reduction of population densities were $63\%$ and about $90\%$ in the P-I and P-II plot respectively when the agent was sprayed on the herbacious plantation. (4) The rates of gall formation were $32.8\%,\;40.8\%\;and\;59.4\%$ in the spray plots of F-I, F-II, and F-III respectively, and there was no significant difference among the upper, middle and lower parts of the treated host plant in the rates of gall formation. (5) The effective stage of sticky agent application were considered as before one week from the peak emergence period in tile all types of treatment.

• Korean journal of applied entomology
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• v.18 no.4 s.41
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• pp.161-167
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• 1979

The study has been carried to investigate a possibility to control several major insect pest of forest by microbial pathogens existing in nature as one of the biological control measure. Microorganisms including polyhedral virus isolated from diseased fall webworm were total of 4 kinds pathogenic microbes among these 4 kinds were polyhedral virus and Bacillus .species. Control effect of these two pathogens appeared to be $70.6\%$ and $49.5\%$, respectively, when they were compared with those of control plot that was $27.8\%$. Each one of bacterium species and fungus species were isolated from diseased Japanese alder leaf beetle. Pathogenisity to the healthy beetle was recognized by the fungus species, while the bacterium showed none of pathogenisity. The fungus was identified as Beauveria sp. and its effect on the beetle control was $96.2\%$ while untreated plot showed $49.2\%$ of dead beetles in the same period. Fifteen species of microbes were isolated from diseased larvae of pine gall midge. Six species out of 15 showed certain level of insecticidal effect to the larvae of the insects. The highest efficiency was showed by a fungus species, Fusarium sp. and was followed by Bacillus SP. I, Spicaria sp. pathogens isolated from larvae of pine gall midge did not affected to both of Japanese alder leaf beetles and fall webworms in any means.

• Journal of Korean Society of Forest Science
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• v.85 no.3
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• pp.439-442
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• 1996

In this study, we tried to figure out the ecology of Masakimyia pustulae Yukawa et Sunose which attacts evergreen euonymus and results in scenic damage caused by early falling of leaves and may increase the susceptibility for anthrocnose. In addition, we tried to develope a method for the control of the insect to prevent spreading of the damage by use of insecticide. The results obtained from the study were as follows : 1. Masakimyia pustulae emerged from late April to late May at Chuncheon area. Average length of the adult insects was $1.88{\pm}0.43mm$, and the life span of them reached about 11 hours. The sex ratio of the insect was 56 : 44(female : male), and they ovipositied $90{\pm}28$ eggs. The shape of eggs was oval with average length $0.32{\pm}0.013mm$. The length of larvae averaged $1.64{\pm}0.25mm$ and started pupation from mid-March, which took about 40~50 days, although there was variance due to temperature variation in each spring. 2. The 3% powder of carbofuran treatment by burying $200g/m^3$ of the insecticide around the damaged tree during mid-March with additional burying about a week after pupation showed good result for the control of the insect, which resulted in more than 96.2% removal of the insects.

• Korean journal of applied entomology
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• v.19 no.1 s.42
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• pp.5-9
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• 1980

Some insecticides were evaluated on the effect of single ULV foliar spray in the control of the pine gall midges (Thecediplosis japonensis Uchida et Inouye) when the formulated (undiluted) and/or diluted insecticides were applied by ULV Sprayer (Battery-type of 12 voltage, devised by Union Carbide) With the formulated insecticide spray (30ml per plot; ten pine trees of 1.5 to 2m in height), the order of control effectiveness was Salithion (Ec 25), $Sumithion^{(R)}$ (ULV 80), Dimethoate (Ec 50), $Sevin\;oil^{(R)}$ (ULV 50), $Zolone^{(R)}$ (Ec 25) and $Folimat^{(R)}$ (Ec 50). However, except Zolone, other insecticides tested caused relatively severe phytotoxicity on the pine needles in all treatments. The dilluted insecticides (200ml Per Plot) of Salithion and Dimethoate with 10,20 and 40 times of water solution showed better control effect than with the formulated insecticides, and no phytotoxicity was observed. Salithion was more effective than Dimethoate. In conclusion, the desirable results in the pine gall midge control in this experiment were obtained by single ULV foliar spray of Salithion with 10 to 20 times of water solution, and the feasible timing of insecticide application would be from late in May to early in June.