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Experimental Studies on Pathogenicities and Developmental Stages of Nosema apis(Zander, 1909) (꿀벌에 기생(寄生)하는 Nosema apis의 병원성(病原性)과 발육단계(發育段階)에 관한 연구(硏究))

  • Kang, Yung Bai;Kim, Dong Sung;Jang, Du Hwan
    • Korean Journal of Veterinary Research
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    • v.16 no.1
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    • pp.11-25
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    • 1976
  • Studies on pathogenicities and developmental stages of Nosema apis (Zander, 1909) were carried out through artificial infection to Nosema free honey bees with various levels of spores isolated from local honey bee colony. The results obtained were summarized as follows: 1. The clinical symptoms were observed as dysentery, enteritis of mid-gut (enlargement and decoloration), crawling posture and shortening of the longevity of worker bees in the rearing honey bee colony inoculated with the spores. 2. Number of spores harvested from laboratory rearing honey bees were progresively increased to 4 weeks after inoculation. The regression equations and coefficients of correlations to various spore levels were as follows in each treatment colony. Colony 1. ($$1,000{\times}10^4spores/ml$$) $$y_{c1}=471{\times}10^{4}x+454{\times}10^4(r=0.65^*$$) Colony 2. ($$500{\times}10^4spores/ml$$) $$y_{c2}=340{\times}10^{4}x+207.8{\times}10^4(r=0.99^{**}$$) Colony 3. ($$100{\times}10^4spores/ml$$) $$y_{c3}=150{\times}10^{4}x+84.2{\times}10^4(r=0.99^{**}$$) Colony 4. ($$10{\times}10^4spores/ml$$) $$y_{c4}=13.8{\times}10^{4}x+13{\times}10^4(r=0.98^{**}$$) 3. Average longevity of worker bees artificially infected with Nosema apis was shortened as 21.7~43.8% compare to the control. (p<.05, p<.01) 4. The spores which were isolated from honey bee colony infected with Nosema disease were ovoid or spherical form, and measured, as a rule, from $4.7{\mu}m$ to $6.1{\mu}m$ (mean $5.3{\mu}m$) in length and from $2.4{\mu}m$ to $3.2{\mu}m$ (mean $2.9{\mu}m$) in width. 5. In the mid-gut of honey bees, the spore was progresively germinated and became trophozoite stage. The trophozoites were grown to meronts and their binary fission were begun. The divided two sporoblasts were developed to the spores which had elastic membrane. The new spores were shed in excreta of honey bees 10~15 day after inoculation at $25{\pm}2$ centigrade. 6. The ultrastructure of spore membrane consisted of three layers, such as, outer, middle and inner layer. The sporoplasm consisting lamellar structure occupied only anterior part of the spore and was often extended to posterior direction where definite vacuoles and a polar filament was able to detect.

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Effect of Soil Water on the Change in Soil pH and Nutrients Movement under Rainfed Condition in Bare Soil (토양수분(土壤水分) 조건(條件)에 따른 pH 변화(變化)와 무관수(無灌水) 나지구(裸地區)에서의 양분이동(養分移動))

  • Ryu, Kwan-Shig
    • Korean Journal of Soil Science and Fertilizer
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    • v.27 no.4
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    • pp.263-268
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    • 1994
  • Field microplot(D 20cm, L 85cm) experiment filled with Bonryang sandy loam soil(Typic Udifluvents) was conducted to obtain quantitative information on the change of soil pH under different soil moisture regime and ladino clover cultivation, and the movement of applied nutrient under the rainfed bare soil condition. Slaked lime increased soil pH of the applied layer. $NH_4-N$ evolved from urea hydrolysis was more effective in raising the subsoil pH rather than the exchangeable Mg and Ca. Under the different soil miosture condition downward movement of Cl and $NO_3-N$ lowered subsoil pH during the middle of ladino clover growth. Leaching of CI and $NO_3-N$ down to the out of the soil microplot and increasing movement of Ca and Mg into the subsoil produced. a slight increase of subsoil pH during the late crop growth. The mean downward movement of applied nutrient 5.5 months after field installation of microplot in the bare soil under rainfed condition(1207mm) was in the order : Cl= more than 80.0>Inorganic nitrogen= 75.8>K= 67.3>Mg=62.7>Ca=18.1>P=2.3cm respectively.

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Development of Digital-Image-Correlation Technique for Detecting Internal Defects in Simulated Specimens of Wind Turbine Blades (풍력 블레이드 모의 시편의 내부 결함 검출을 위한 이미지 상관법 기술 개발)

  • Hong, Kyung Min;Park, Nak Gyu
    • Korean Journal of Optics and Photonics
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    • v.31 no.5
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    • pp.205-212
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    • 2020
  • In the performance of a wind turbine system, the blades play a vital role. However, they are susceptible to damage arising from complex and irregular loading (which may even cause catastrophic collapse), and they are expensive to maintain. Therefore, it is very important both to find defects after blade manufacturing is completed and to find damage after the blade is used for a certain period of time. This study provides a new perspective for the detection of internal defects in glass-fiber- and carbon-fiber-reinforced panels, which are used as the main materials in wind turbine blades. A gap or fracture between fiber-reinforced materials, which may occur during blade manufacturing or operation, is simulated by drilling a hole 5 mm in diameter in the middle layer of the laminated material. Then, a digital-image-correlation (DIC) method is used to detect internal defects in the blade. Tensile load is applied to the fabricated specimen using a tensile tester, and the generated changes are recorded and analyzed with the DIC system. In the glass-fiber-reinforced laminated specimen, internal defects were detected from a strain value of 5% until the end of the experiment, while in the case of the carbon-fiber-reinforced laminated specimen, internal defects were detected from 1% onward. It was proved using the DIC system that the defect was detected as a certain level of strain difference developed around the internal defects, according to the material properties.

The Study on the Phytoplankton Bloom and Primary Productivity in Lake Shihwa and Adajcent Coastal Areas (시화호와 시화호 주변 해역 식물플랑크톤의 대증식과 일차 생산력에 관한 연구)

  • Choi, Joong-Ki;Lee, Eun-Hee;Noh, Jae-Hoon;Huh, Sung-Hoi
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.2 no.2
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    • pp.78-86
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    • 1997
  • To clarify the phytoplankton blooms in Lake Shihwa after the construction of a dyke, a study on the environmental factors, the distribution of chlorophyll-a, phytoplankton standing stocks, dominant species and primary productivity was carried out in Lake Shihwa and adjacent coastal areas from October, 1995 to August, 1996. Lake Shihwa is brackish water with mixing of freshwater from tributaries and the remaining salt water at the bottom. The dense phytoplankton bloom of average value of 168.6 ${\mu}gChl-a\;l^{-1}$ have occurred throughout the year in Lake Shihwa which is eutrophicated by the large input of nutrients from inflowing 5 tributaries and Shihwa Industrial Complex. The major organisms of algal bloom in Lake Shihwa were diatoms, Cyclotella atomus, Nitzschia sp. and Chaetoceros sp. in autumn and winter, and dinoflagellate Prorocentrum minimum and Chrysophyceae in spring and summer. The autumn and winter diatom blooms were limited by the depletion of silicate in the lake. Diatom blooms have occurred in the coastal areas adjacent to Shihwa lake from winter to summer due to the inflow of nutrient rich-water from Lake Shihwa. The primary productivities in the Lake Shihwa ranged from 2,653 mgC $m^{-2}\;day^{-1}$ to 9,505 mgC $m^{-2}\;day^{-1}$ with an average of 3,972 mgC $m^{-2}\;day^{-1}$. However, most of the high primary production was limited to the shallow euphotic zone due to the inhibition of light penetration. The primary productivities during autumn and winter were limited by the depletion of silicate. Lack of photosynthesis and the decomposition of falling organic matter under the middle of water column accelerated the depletion of dissolved oxygen in the bottom layer.

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Application of Algal Growth Potential Test (AGPT) on the Water Quality of the Chinyang Reservoir and the Nam River (진양호와 남강의 수질에 대한 Algal Growth Potential Test (AGPT) 적용)

  • Lee, Ok-Hee;Cho, Kyung-Je;Shin, Jae-Ki
    • Korean Journal of Ecology and Environment
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    • v.36 no.1 s.102
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    • pp.57-65
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    • 2003
  • The algal growth potential test (AGPT) bioassay were conducted to assess the water quality and fertility in the Chinyang Reservoir and the lower part of the Nam River from August 2000 to July 2001, The AGPT value of the Chinyang Reservoir ranged from 0 to 23.4 mg dw $1^{-1}$, while 79% of the algae cultivation have not grown. The AGPT value was in proportion to phosphorus concentration of the water, and it was less when chlorophyll-a was high. This value was higher in the middle and lower layers than in the upper layer, and in the inflow part where the water is shallower than in the lacustrine. The AGPT value has increased in the whole reservoir in August${\sim}$September when the water volume is high. In contrast, the AGPT value in the Nam River varied greatly compared to that of the reservoir, and ranged from 0 to 252.0 mg dw $1^{-1}$ and 65% of the algae cultivation have grown. The value was less than 10 mg dw $1^{-1}$ in the upstream, over the point where the treated wastewater discharged. It was 57 mg dw $1^{-1}$ on the average in the downstream, except in March and July when the discharged water influenced greatly, exceeding the hypertrophic condition. The result of AGPT shows the differences in the time and space on the reservoir and the streams. The AGPT value has increased in July${\sim}$September, and in December in the inflow part of the reservoir; in March and August${\sim}$December in the lower part; and in January, May, and November in the streams. AGPT is useful not only in defining the influence of the limiting nutrients on the algal growth, but also in evaluating the nutrients fertility in the inland water.

Distribution of Water Temperature and Common Squid Todarodes pacificus Paralavae around Korean Waters in 2013, 2014 (2013-2014년 한국주변해역 수온과 살오징어 유생분포)

  • Kim, Yoon-Ha;Lee, Chung Il
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.22 no.1
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    • pp.11-19
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    • 2016
  • Field observation for oceanic conditions and paralarvae of the common squid, Todarodes pacificus in Korean waters were sampled with the Bongo net (diameter: 60 cm, mesh size: $333{\mu}m$) by using oblique tow method with the oceanographic research vessel (Tamgu 12 and Tamgu 20) around Korean waters (middle of the Yellow Sea, northern part of the East China Sea, East Sea) in 2013 and 2014 was carried out. The observation in the Yellow Sea and the northern part of the East China Sea was done in August, 2013 and in the East Sea it was repeated at seven times from June, 2013 to September, 2014. The paralarvae in August of 2013 was not found in the Yellow Sea and one paralarvae was found in the northern part of the East China Sea. In the East Sea, 39 paralarvae during whole observation period were found, mantle length of paralarvae was from 1.7 to 13.5 mm. Surface water temperature in the Yellow Sea was $30^{\circ}C$, and cold water mass lower than $10^{\circ}C$ was occupied in the deep layer than 30 m. In the northern part of the East China Sea, surface water temperature was $31^{\circ}C$, and higher water temperature above $20^{\circ}C$ was found in deeper than 50 m. In the East Sea, optimum temperature for survival, $15-24^{\circ}C$, was existed shallower than 75 m.

Numerical Analysis of Synchronous Edge Wave Known as the Driving Mechanism of Beach Cusp (Beach Cusp 생성기작으로 기능하는 Synchronous Edge Wave 수치해석)

  • Lee, Hyung Jae;Cho, Yong Jun
    • Journal of Korean Society of Coastal and Ocean Engineers
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    • v.31 no.6
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    • pp.409-422
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    • 2019
  • In this study, we carried out the 3D numerical simulation to investigate the hydraulic characteristics of Synchronous Edge wave known as the driving mechanism of beach cusp using the Tool Box called the ihFoam that has its roots on the OpenFoam. As a wave driver, RANS (Reynolds Averaged Navier-Stokes equation) and mass conservation equation are used. In doing so, we materialized short-crested waves known as the prerequisite for the formation of Synchronous Edge waves by generating two obliquely colliding Cnoidal waves. Numerical results show that as can be expected, flow velocity along the cross section where waves are focused are simulated to be much faster than the one along the cross section where waves are diverged. It is also shown that along the cross section where waves are focused, up-rush is moving much faster than its associated back-wash, but a duration period of up-rush is shortened, which complies the typical characteristics of nonlinear waves. On the other hand, due to the water-merging effect triggered by the redirected flow toward wave-diverging area at the pinacle of run-up, along the cross section where waves are diverged, offshore-ward velocity is larger than shore-ward velocity at the vicinity of shore-line, while at the very middle of shoaling process, the asymmetry of flow velocity leaned toward the shore is noticeably weakened. Considering that these flow characteristics can be found without exception in Synchronous Edge waves, the numerical simulation can be regarded to be successfully implemented. In doing so, new insight about how the boundary layer streaming occur are also developed.

Seasonal Variation of Surface heat budget and Wind Stress Over the Seas Around the Korean Peninsula (한반도주위 해양에서 의 해면 열수지와 응력의 계절변화)

  • 강인식;김맹기
    • 한국해양학회지
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    • v.29 no.4
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    • pp.325-337
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    • 1994
  • The distributions of heat and momentum fluxes on the surface over the oceans around the Korean Peninsula are obtained based on the surface-layer flux model of Kim and Kang (1994), and their seasonal variations are examined in the present study. the input data of the model is the oceanatmosphere data with a grid interval of 2$^{\circ}$ in longitude and latitude. The atmosphere data, which are the pressure, temperature, and specific humidity on the 1000 mb level for 3 year period of 1985∼1987, are obtained from the European center for Medium Range Forecast. The sea surface temperature (SST) is obtained from National Meteorological Center (NMC). The solar insolation and longwave radiation on the ocean surface are obtained, respectively, from the NASA satellite data and based on an emprical formula. It is shown from the net heat flux that the oceans near Korea lose heat to the atmosphere in January and October with the rates of 200∼ 400 Wm/SUP -2/ and 100 Wm/SUP -2/, respectively. But the oceans are heated by the atmosphere in April and July with about the same rate of 100 Wm/SUP -2/. The annualmean net heat flux is negative over the entire domain except the northern part of the Yellow Sea. The largest annual-mean cooling rate of about 120 Wm/SUP -2/ is appeared off the southwest of Japan. In the East Sea, the annual-mean cooling rate is 60∼90 Wm/SUP -2/ in the southern and northern parts and about 30 Wm/SUP -2/ in the middle part. The magnitude of wind stress in january is 3∼ 5 times bigger than those of the other months. As a result, the spatial pattern of annual-mean wind stress is similar to that of January. It is also shown that the annual-mean wind stress curl is negative. in the East China Sea and the South Sea,but it is positive in the northern part of the Yellow Sea.In the East sea,the stress curl is positive in the southeast and northern parts and negative in the northwestern part.

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Study on the growth of 4H-SiC single crystal with high purity SiC fine powder (고순도 SiC 미분말을 적용한 4H-SiC 단결정 성장에 관한 연구)

  • Shin, Dong-Geun;Kim, Byung-Sook;Son, Hae-Rok;Kim, Moo-Seong
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.29 no.6
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    • pp.383-388
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    • 2019
  • High purity SiC fine powder with metal impurity contents of less than 1 ppm was synthesized by improved carbothermal reduction process, and the synthesized powder was used for SiC single crystal growth in RF heating PVT device at temperature above 2,100℃. In-situ x-ray image analyzer was used to observe the sublimation of the powder and single crystal growth behavior during the growth process. SiC powder was used as a source of single crystal growth, exhausted from the outside of the graphite crucible at the growth temperature and left graphite residues. During the growth, the flow of raw materials was concentrated in the middle and influenced the growth behavior of SiC single crystals. This is due to the difference in temperature distribution inside the crucible due to the fine powder. After the single crystal growth was completed, the single crystal ingot was cut into a 1 mm thick single crystal substrate and finely polished using a diamond abrasive slurry. A dark yellow 4H-SiC was observed overall of single crystal substrate, and the polycrystals generated in the outer part may be caused by the incorporation of impurities such as the bubble layer mixed in the process of attaching the seed crystal to the seed holder.

Effect of Hominis Placenta on cutaneous wound healing in normal and diabetic mice

  • Park, Ji-Yeun;Lee, Jiyoung;Jeong, Minsu;Min, Seorim;Kim, Song-Yi;Lee, Hyejung;Lim, Yunsook;Park, Hi-Joon
    • Nutrition Research and Practice
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    • v.8 no.4
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    • pp.404-409
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    • 2014
  • BACKGROUND/OBJECTIVES: The number of diabetic patients has recently shown a rapid increase, and delayed wound healing is a major clinical complication in diabetes. In this study, the wound healing effect of Hominis placenta (HP) treatment was investigated in normal and streptozotocin-induced diabetic mice. MATERIALS/METHODS: Four full thickness wounds were created using a 4 mm biopsy punch on the dorsum. HP was injected subcutaneously at the middle region of the upper and lower wounds. Wounds were digitally photographed and wound size was measured every other day until the 14th day. Wound closure rate was analyzed using CANVAS 7SE software. Wound tissues were collected on days 2, 6, and 14 after wounding for H/E, immunohistochemistry for FGF2, and Masson's trichrome staining for collagen study. RESULTS: Significantly faster wound closure rates were observed in the HP treated group than in normal and diabetes control mice on days 6 and 8. Treatment with HP resulted in reduced localization of inflammatory cells in wounded skin at day 6 in normal mice and at day 14 in diabetic mice (P < 0.01). Expression of fibroblast growth factor (FGF) 2 showed a significant increase in the HP treated group on day 14 in both normal (P < 0.01) and diabetic mice (P < 0.05). In addition, HP treated groups showed a thicker collagen layer than no treatment groups, which was remarkable on the last day, day 14, in both normal and diabetic mice. CONCLUSIONS: Taken together, HP treatment has a beneficial effect on acceleration of cutaneous wound healing via regulation of the entire wound healing process, including inflammation, proliferation, and remodeling.