• Title/Summary/Keyword: microscopic characteristics

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Effect of ${\beta}-Glucan$ on Gelatinization of Barley Starch (${\beta}-Glucan$이 보리 전분의 호화에 미치는 영향)

  • Choi, Hee-Don;Seog, Ho-Moon;Kim, Sung-Ran;Park, Yong-Kon;Lee, Cherl-Ho
    • Korean Journal of Food Science and Technology
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    • v.35 no.4
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    • pp.545-550
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    • 2003
  • The effect of ${\beta}-glucan$ on gelatinization of barley starch was studied. By the rapid visco-analyzer measurement, gelatinization of starch became rapid and viscosity increased largely on the RVA pattern by addition of ${\beta}-glucan$ to starch. The results of differential scanning calorimeter showed that molecular structure of starch was getting stabilized through shifting up of gelatinization temperature and increase in enthalpy by addition of ${\beta}$-glucan. X-ray diffraction pattern also showed the same results as differential scanning calorimeter. But it was revealed that addition of ${\beta}$-glucan to starch didn't affect characteristics such as microscopic observation, solubility, swelling power, and iodine binding properties during gelatinization of starch.

The Measurement of Minimum Ignition Energy and Explosion Limit for Pine Tree Dust (소나무 분진의 최소착화에너지와 폭발한계 측정에 관한 연구)

  • Choi Il-Gon;Cho Il-Keon;Mok Yun-Soo;Lee Dong-Hoon;Choi Jae-Wook;Ha Dong-Myeong
    • Journal of the Korean Institute of Gas
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    • v.2 no.2
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    • pp.55-60
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    • 1998
  • In this study, we investigated the dust explosion characteristics by determining minimum ignition energy and explosion limit for this experiment, we used pine-tree dust which was used widely for the filler of thermosetting resin. The experiment was accomplished according to the variation of discharge gap, dust concentration, particle size and humidity. The result of this experiment are as follows; (1) The relation between the discharge gap and ignition energy was that ignition energy decreased according as the discharge gap became small, but increased when the discharge gap was below 4mm and suddenly became infinite when the discharge gap was below. So, we knew that this infinite value was limit discharge gap. (2) When the dust concentration increase and the particle size became microscopic it was easy to explore and in the same particle size, if the humidity increase the minimum ingnition energy decreased.

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Comparative Ultrastructural Study on four Candida species and Cryptococcus neoformans (Candide species와 Cryptococcus neoformans의 전자현미경적 미세구조에 관한 비교 연구)

  • Yoon, Chul-Jong;Kim, Sung-Gwon;Kim, Soo-Sung;Chi, Je-Geun
    • Applied Microscopy
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    • v.23 no.2
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    • pp.97-106
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    • 1993
  • This study was done to elucidate the electron microscopic characteristics of certain pathogenic fungi. Four Candida species, (C. albicans, C. tropicalis, C. parapsilosis and C. glabrate) and Cryptococcus neoformans were cultured for 3 days at $30^{\circ}C$ in the Sabouraud dextrose medium. After incubation, they were stored at $4^{\circ}C$ for 24hours. Fine structures were analyzed by morphometry, and Tukey's HSD test was used for statistics. On scanning electron microscopy C. albicans and C. neoformans were similar in size but different in shape, showing sphero-shape or ovalo-shape in C. neoformans. Surface of C. neoformans was coarse and spiny, but Candida species examined were uniformly smooth. In size, C. glabrata was the smallest among them. Budding scar as seen on the surface of Candida species by the number ranging from 1 to 7. Cryptococcus neoformans showed one or two budding scar. On transmission electron microscopy the cytoplasm of most yeast cells showed plentiful glycogen particles, mitochondria, peroxisomes and vacuoles. However, cell walls were different among four Candida species and Cryptococcus neoformans. The cell wall of Candida species consisted of fibrous layer, that was electron dense layer and transparent layer, in contrast to Cryptococcus neoformans consisted of electron dense layer with lamellar structure. This layer was two times thicker than that of Candida species. The outer layer of cell wall was of radiating pattern.

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Pathological Interrelations of Soil-Borne Diseases in Cucurbits Caused by Fusarium Species and Meloidogyne incognita

  • Seo, Yunhee;Kim, Young Ho
    • The Plant Pathology Journal
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    • v.33 no.4
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    • pp.410-423
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    • 2017
  • Pathological interrelations of two soil-borne diseases in cucurbits (watermelon, oriental melon, shintosa and cucumber) caused by Fusarium isolates (FI) and the root-knot nematode (RKN), Meloidogyne incognita were characterized by the fusarium disease severity index (DI), RKN gall index (GI) and eggmass index (EI) in inoculation tests using FI and RKN. Virulence of FI as determined by DI at 4 weeks after inoculation was mostly in the higher order of Fusarium proliferatum F6, F5 and Fusarium oxysporum f. sp. melonis or Fusarium oxysporum f. sp. niveum with no significant differential interactions among the cucurbits and RKN co-infection. Significant increases of DI due to RKN coinfection were noticed in watermelon and oriental melon infected with F. proliferatum isolates, suggesting the DI increase due to RKN coinfection may depend upon the virulence of FI relative to aggressiveness of RKN on the cucurbits. For the coinfection of FI and RKN, GI and EI were mostly reduced logarithmically with the increase of DI, largely more in EI than GI, in all cucurbits except for shintosa. Microscopic examination of the root tissues showed histopathological features characteristic to infection types; formation of fungal hyphae and/or spores and plant defense structures (tyloses and mucilage) in variable degrees and formation of giant cells at variable developmental stages and with variable cytoplasmic depletion or degeneration which were visualized in relations with the values of DI, GI and EI. These findings will be helpful to develop control strategies of the soil-borne disease complex based on their pathological characteristics.

A Case of MALT Lymphoma in Parotid Gland Duct (이하선관에 발생한 MALT 림프종 1예)

  • Kim, Ki Yup;Yang, Won Yong;Kwon, Seok Min;Kang, Sang Yoon
    • Archives of Plastic Surgery
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    • v.36 no.5
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    • pp.663-666
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    • 2009
  • Purpose: Primary malignant lymphomas of the salivary glands are uncommon. The parotid gland was most frequently involved, followed by the submandibular gland, minor salivary gland and sublingual gland. The most common subtype is mucosa - associated lymphoid tissue(MALT) lymphoma. We experienced a case of salivary MALT lymphoma involving parotid gland duct, so report a case with a review of the literature. Methods: A 65 year old female presented with a palpable mass on the left side of her cheek. There was no clinical or laboratory evidence of pre - existing autoimmune disease. Preoperative facial and neck CT with contrast showed $2.1{\times}1.7cm$ sized, ill defined, homogeneous low density mass near left masseter muscle, and no evidence of other enlarged lymph nodes. Results: At operation, a yellowish oval shaped mass was found slightly adhered to middle portion of the parotid gland duct, meaduring $2{\times}1.5{\times}0.7cm$. Microscopic finding showed that centrocyte - like cells, monocyte B cells and plasma cells were diffusely infiltrated. Immunophenotyping was preformed on fixed section. The majority of the small cells were immunoreactive for the B cell marker CD20. Based on the typical histological findings supported by immunostaining, the mass was defined as MALT lymphoma. Conclusion: We report that very rare case of MALT lymphoma involving parotid gland duct in 65 year old female patient was experienced with clinical characteristics, histologic features and references.

Preparation of Cross-sectional Specimen for High Resolution Observation of Coating Structure and Visualization of Styrene/butadiene Latex Binder (고배율 도공층 구조 및 S/B latex 분포 분석을 위한 도공층 횡단면 제작)

  • Kim, Chae-Hoon;Youn, Hye-Jung;Lee, Hak-Lae
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.44 no.4
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    • pp.16-24
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    • 2012
  • To characterize the coating structure, diverse methods such as mercury intrusion, nitrogen adsorption and oil absorption methods have been developed and widely employed. These indirect techniques, however, have some limitation to explain the actual coating structure. Recently microscopic observation methods have been tried for analyzing structural characteristics of coating layers. Preparation of the undamaged cross section of a coating layer is essential for obtaining high quality image for analysis. In this study, distortion-free cross-section of the coating layer was prepared using a grinding and polishing technique. The coated paper was embedded in epoxy resin and cured. After curing the resin block it was ground with abrasive papers and then polished with diamond particle suspension and nylon cloth. Polished coating layer was sufficient enough to obtain undamaged cross sectional images with scanning electron microscope under backscattered electron image mode. In addition, the SEM images allowed distinction of the coating layer components. Also S/B latex film formed between pigment particles was visualized by osmium tetroxide staining. Pore size distribution and pore orientation were evaluated by image analysis from SEM cross-sectional images.

Production Method of Wooden Seated Bodhisattva from Gwaneumsa Temple, Wando (완도 관음사 목조보살좌상의 제작 방법)

  • Kwak, Eungyung;Lee, Hyejin;Yu, Sohyun;Son, Jongmin
    • Conservation Science in Museum
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    • v.18
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    • pp.77-92
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    • 2017
  • This study is based on a scientific analysis of the production method of the wooden seated Bodhisattva statue from Gwaneumsa Temple in Wando, which is currently housed at Chonnam National University Museum. The purpose was to investigate the characteristics of the statue's production through an X-ray inspection of the wooden timbers that make up its base material, a composition analysis of the surface layers, and microscopic observation. The votive prayers found together with the statue allowed the precise dating of the artifact to the sixteenth century, during the Joseon Dynasty. The X-ray transmission identified the statue as being made using the "ilmokjo"(一木造) technique, which means that the entirety of the statue excluding the right hand was produced using a single block of wood. The specimen analysis of the naturally exfoliated surface layer revealed that the current surface was coated with brass to restore the original gilt layer. These research findings added an interesting case to the existing related research and reaffirmed the academic value of this statue.

Markers of Collagen Change in Chronic Secondary Renal Disease Model in Rat (만성 속발성 신질환 모델동물에서 콜라젠 변화의 지표)

  • 남정석;김기영;이영순
    • Toxicological Research
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    • v.12 no.2
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    • pp.213-221
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    • 1996
  • In order to develop a suitable secondary renal disease model and diagnostic markers of renal disease in the rat, the change of PIIIP (aminoterminal procollagen III peptide) in serum and hydroxyproline levels in the renal tissue that reflect the synthesis of extracellular matrix (ECM) during development of experimental renal diseases were observed. Two types of experimental primary diseases, diabetes mellitus administrated by streptozotocin (STZ, 75 mg/kg, i.p.) and liver cirrhosis produced by bile duct ligation/scission (BDL/s) operation, were induced. The hydroxyproline level increased according to the high PIIIP and NCl(carboxyterminal procollagen IV peptide) in Western blot analysis as early as 1 week in the STZ treated-rat kidney. Increased renal ECM was observed at 15 weeks in STZ and BDL/s model under the microscopic examination. High PAS positive reaction was found in capillary basement membrane in STZ treated-rats and mesangium in BDL/s operated rats at this time, showing the histological characteristics of diabetic nephropathy and cirrhotic glomerulonephritis in human, respectively. Such secondary renal failure were supported by additional tests including urinalysis and renal function test. The serum PIIIP detected by ELISA was a useful parameter to estimate synthesis rate of renal ECM during development of renal disease without extrarenal fibrosis i.e. liver cirrhosis in rats. This study is proposed that STZ treatment or BDL/s operation may be a suitable experimental animal model for the induction and development of chronic secondary renal diseases. Morover, it was found that hydroxyproline level in renal tissues was a good parameter of the change of renal ECM at the early stage of the diseases without apparent histological changes. Especially, serum PIIIP could be a choice as a diagnostic or prognostic marker during the development of renal diseases in rats.

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AE Characteristics on the Damage Behavior of TiNi/A16061 Shape Memory Alloy Composites at High Temperature (TiNi/A16061 형상기억복합재료의 고온에서의 손상거동에 대한 AE 특성)

  • Lee, Jin-Kyung;Park, Young-Chul;Ku, Hoo-Taek
    • Journal of the Korean Society for Nondestructive Testing
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    • v.22 no.1
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    • pp.45-52
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    • 2002
  • It has been known that tensile residual stresses occurring by the thermal expansion coefficient mismatch between fiber and matrix is a cause of the weak strength of metal matrix composites(MMCs). In order to solve this problem, TiNi alloy fiber was used as a reinforced material in TiNi/A16001 shape memory alloy composite in this study. TiNi alloy fiber improves the tensile strength of the composite by causing compressive residual stress in matrix on the basis of its shape memory effect. Pre-strain was imposed to generate the compressive residual stresses inside the TiNi/A16001 shape memory alloy composites. AE technique was used to quantify the microscopic damage behavior of the composite at high temperature. The effect of applied pre-strains on the AE behavior was also evaluated.

Distribution of Toxic and Non-toxic Microcystis in Korean Water Supply (국내 주요 상수원지에서 독성 및 비독성 Microcystis의 분포 특성)

  • Lee, Kyung-Lak;Shin, Yuna;Lee, Jaean;Lee, Jae-Kwan;Kim, Han Soon
    • Korean Journal of Ecology and Environment
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    • v.49 no.4
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    • pp.393-399
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    • 2016
  • This study was conducted to investigate whether the presence of mcy gene and microcystin production are related to morphological characteristics of Korean Microcystis species. We isolated 6 different species of Microcystis (M. aeruginosa, M. ichthyoblabe, M. flos-aquae, M. novacekii, M. viridis and M. wesenbergii) from drinking water supply dams (Yeongchun, Ankei, Gachang), and used microscopic method for morphological identification, molecular method for amplifying a partial region of mcyB gene and ELISA method for microcystin analysis. In the present study, 80% of M. aeruginosa strains contained mcy gene, followed by 45% (10 strains) of M. icthyoblabe, 33% (1 strain) of M. wesenbergii, and 11% (4 strains) of M. flos-aquae. Each percentage of mcy gene in Microcystis morphospecies was similar to that of microcystin production in Microcystis morophospecies. In conclusion, the present study shows that molecular method using mcy gene primers can be used as an indirect indicator for the monitoring of toxic cyanobacteria (Microcystis).