• Journal of Mushroom
• /
• v.22 no.2
• /
• pp.48-52
• /
• 2024

This study investigated the microscopic characteristics and genetic relationships of Ganoderma applanatum fruiting bodies. Basidiospores were brown, ellipsoid, and had one or two large vacuoles and a double wall. The surface of basidiospores was smooth or wrinkled and most had numerous small and shallow holes. The length and width of basidiospores of Ganoderma applanatum isolates GBGA-01, GBGA-02, ASI 50167, ASI 52821, ASI 52822, ASI 52823, and ASI 53399 were on average 7.6×4.8 ㎛, 7.9×4.6 ㎛, 7.7×4.9 ㎛, 8.2×5.3 ㎛, 7.7×5.0 ㎛, 8.0×4.9 ㎛, and 7.9×4.9 mm, respectively. In contrast, the basidiospores of Ganoderma lucidum isolate ASI 7125 were 7.7×5.2 ㎛. Using the universal ITS1/ITS4 primer set, the ITS region of the isolates were amplified and sequenced. The ITS sequences were very closely related to G. applantum isolate GBGA-01, GBGA-02, ASI 50167, ASI 52821, ASI 52822, ASI 52823 and ASI 53399, but were not the same species. Whereas, G. lucidum isolate ASI 7125 belongs to different group.

• Clinical Endoscopy
• /
• v.57 no.4
• /
• pp.434-445
• /
• 2024

Pancreatic cystic lesions (PCLs) have increased in prevalence due to the increased usage and advancements in cross-sectional abdominal imaging. Current diagnostic techniques cannot distinguish between PCLs requiring surgery, close surveillance, or expectant management. This has increased the morbidity and healthcare costs from inappropriately aggressive and conservative management strategies. Endoscopic ultrasound (EUS) needle-based confocal laser endomicroscopy (nCLE) allows for microscopic examination and delineation of the surface epithelium of PCLs. Landmark studies have identified characteristics distinguishing various types of PCLs, confirmed the high diagnostic yield of EUS-nCLE (especially for PCLs with an equivocal diagnosis), and shown that EUS-nCLE helps to change management and reduce healthcare costs. Refining procedure technique and reducing procedure length have improved the safety of EUS-nCLE. The utilization of artificial intelligence and its combination with other EUS-based advanced diagnostic techniques would further improve the results of EUS-based PCL diagnosis. A structured training program and device improvements to allow more complete mapping of the pancreas cyst epithelium will be crucial for the widespread adoption of this promising technology.

• Parasites, Hosts and Diseases
• /
• v.62 no.2
• /
• pp.238-242
• /
• 2024

Strongyloidiasis is a chronic infection caused by the intestinal nematode parasite Strongyloides stercoralis and is characterized by a diverse spectrum of nonspecific clinical manifestations. This report describe a case of disseminated strongyloidiasis with urination difficulty, generalized weakness, and chronic alcoholism diagnosed through the presence of worms in the urinary sediment. A 53-year-old man was hospitalized for severe abdominal distension and urinary difficulties that started 7-10 days prior. The patient also presented with generalized weakness that had persisted for 3 years, passed loose stools without diarrhea, and complained of dyspnea. In the emergency room, approximately 7 L of urine was collected, in which several free-living female adult and rhabditiform larvae of S. stercoralis, identified through their morphological characteristics and size measurements, were detected via microscopic examination. Rhabditiform larvae of S. stercoralis were also found in the patient's stool. During hospitalization, the patient received treatment for strongyloidiasis, chronic alcoholism, peripheral neurosis, neurogenic bladder, and megaloblastic anemia, and was subsequently discharged with improved generalized conditions. Overall, this report presents a rare case of disseminated strongyloidiasis in which worms were detected in the urinary sediment of a patient with urination difficulties and generalized weakness combined with chronic alcoholism, neurogenic bladder, and megaloblastic anemia.

• Journal of Advanced Marine Engineering and Technology
• /
• v.38 no.7
• /
• pp.890-899
• /
• 2014

Austenitic stainless steel is widely used in various industries due to its excellent corrosion resistance. However, Cr carbides precipitation along the grain boundaries after heat treatment or welding may develop Cr depleted zone, which acts as a preferential site for intergranular corrosion attack. To resolve this, carbon stabilizing element such as Ti or Nb are added to suppress formation of Cr carbides. However, there are few reports on corrosion characteristics under seawater environment of the stabilized stainless steel. This study investigated the effects of alloying contents on the electrochemical characteristics in seawater of stainless steel containing stabilizing element(Ti and Nb). To achieve this, the changes on the microstructure due to alloying were observed with microscope, and the electrochemical characteristics were determined by measurement of natural potential and potentiodynamic polarization experiments. The microscopic observation revealed that all specimens had inclusions other than the austenite matrix phase due to the addition of alloying elements. Such inclusions are considered to have different electrochemical characteristics from those of the matrix, and thus a clear distinction was found according to the type of stabilizers and the contents. The results of this study suggest that it is important to consider the effects of alloying contents on the electrochemical characteristics in seawater with the addition of Ti or Nb into austenitic stainless steel.

• Journal of the Korean Wood Science and Technology
• /
• v.34 no.2
• /
• pp.68-77
• /
• 2006

The heartwood of cengal (Neobalanocarpus heimii) is known to have a high degree of decay resistance by virtue of its high extractive content. After 30 years in ground contact an utility pole of this tropical hardwood was found to be degraded only in the surface layers by cavity-forming soft rot fungi. The present work was undertaken 1) to characterize the degradation of cengal heartwood from the aspect of ultrastructure and chemistry and 2) to investigate the correlation between soft rot decay and its extractive microdistribution in wood tissues. The chemical analysis of cengal heartwood revealed the presence of a high amount of extractives as well as lignin. The wood contained a relatively high amount of condensed lignin and the guaiacyl units. Microscopic observations revealed that vessels, fibers and parenchyma cells (both ray and axial parenchyma) all contained extractives in their lumina, but in variable amounts. The lumina of fibers and most axial parenchyma were completely or almost completely filled with the extractives. TEM micrographs showed that cell walls were also impregnated with extractives and that pit membranes connecting parenchyma cells were well coated and impregnated with extractives. However, fungal hyphae were present in the extractive masses localized in cell lumina, and indications were that the extractives did not completely inhibit fungal growth. The extent of cell wall degradation varied with tissue types. The fibers appeared to be more susceptible to decay than vessels and parenchyma. Middle lamella was the only cell wall region which remained intact in all cell types which were severely degraded. The microscopic observations suggested a close correlation between extractive microdistribution and the pattern and extent of cell wall degradation. In addition to the toxicity to fungi, the physical constraint of the extractive material present in cengal heartwood cells is likely to have a profound effect on the growth and path of invasion of colonizing fungi, thus conferring protection to wood by restricting fungal entry into cell walls. The presence of relatively high amount of condensed lignin is also likely to be a factor in the resistance of cengal heartwood to soft rot decay.

• Journal of Yeungnam Medical Science
• /
• v.9 no.2
• /
• pp.224-238
• /
• 1992

Hepatic fibrosis was induced in Sprague-Dawley rats to evaluate the ultrastructural changes of fat-storing cells(Ito cells). For experimental induction of liver fibrosis, the rats were administered intraperitoneally with 0.5ml of 50% $Ccl_4$ solution per Kg body weight, twice weekly for 12 weeks. The rats were sacrified every week. The liver tissues were examined under light and eletron microscopes. And the immunohistochemical study of desmin was also performed. The results were summarized as follows : Light microscopic findings : The cellular infiltrations with inflammatory cells and Kupffer cells developed from 1 week after $Ccl_4$ injection, and were the most severe in 4 weeks. The strong immunoreactivity for desmin was also evident in 4 weeks. The centrilobular necrosis and fibrosis developed from 2 weeks after injection, and the necrosis persisted until 8 weeks. The progress of fibrosis was accompanied by decreases in cellular infiltration and reactivity for desmin, and increased gradual nodular formation was also observed. The cirrhosis was developed after 10 weeks. Electron microscopic findings : An increase in number of fat-storing cells was observed from 1 week after injection. Transitional cells characterized by a depletion of lipid droplets and a hypertrophy of the rER appeared after 2 weeks. The number of transitional cells with abundant collagen fibers in the extracellular spaces increased in 4 weeks. With progression of fibrosis the number of fat-storing cells decreased and proliferating fibroblasts with dilated rER were observed. According to these results it was revealed that there was an apparent transition from fat-storing cells to transitional cells and to fibroblasts. These cells had a few similar characteristics and may belong to the same cell population. Thus it was suggested that fat-storing cells might play an important role in hepatic fibrosis.

• The Korean Journal of Zoology
• /
• v.26 no.4
• /
• pp.253-270
• /
• 1983

Electron microscopic degeneration studies were carried out on the rat's interpeduncular nucleus following lesion on both habenular nuclei to clarify the morphological nature of their presynaptic endings involved in the habenulo-interpeduncular pathway. Two distinct types of interpeduncular neurons were observed: 30% of large neurons and 70% of small ones. Four types of boutons with many characteristics could be differentiated; small spherical vesicle-containing boutons (40%), large spherical vesicle-containing boutons (18%), pleomorphic vesicle-containing boutons (33%), and elongated vesicle-containg boutons (9%). Vesicle containing dendrites were observed as dendro-dendritic synapses and axo-axonal synapses were also found, though rarely. Two to four days after the lesion on both habenular nuclei, the small spherical vesicle-containing boutons were found almost exclusively in the process of degeneration, while the other types were entirely free of degeneration. Therefore, it is inferred that habenural neurons terminate in the interpeduncular nucleus as small spherical vesicle-containing boutons. The neuronal origin of the other bouton types is discussed.

• KSCE Journal of Civil and Environmental Engineering Research
• /
• v.34 no.4
• /
• pp.1269-1278
• /
• 2014

The urban highway, which is a motorway constructed to solve traffic issues, has the characteristic of extremely high damage to life during traffic accidents because the speed of vehicles is higher than typical roadways. In particular, because traffic accidents involving serious injuries hold a very important place among overall traffic accidents, analysis on factors affecting the occurrence of traffic accidents involving serious injuries must be considered with priority when establishing a reduction measure. Therefore, the study built a model that was capable of distinguishing the degree of the factors as part of microscopic analysis for investigating the complex effect of many elements concerning the occurrence of traffic accidents involving serious injuries in urban highways. The results are as follows. First, discriminant model showed a comparatively high level in overall accuracy rates, and, considering the correlation ratio, the models were determined to be valid, as all characteristics of the factors were clearly distinguished. Second, the problems of traffic accidents involving serious injuries on urban highways according to each factor, were clearly drawn out through the discriminant model. Third, the improvement measure for the problems drawn out from the discriminant models were clearly proposed.

• The Journal of The Korea Institute of Intelligent Transport Systems
• /
• v.13 no.5
• /
• pp.1-10
• /
• 2014

This research focuses on the warrants for the Two-Way Left-Turn Lanes (TWLTL). Using a microscopic traffic simulation tool, two key parameters were investigated herewith. One is a wide range of the Design Hourly Volume (DHV), reflective of recent Korean roadway volume characteristics, that is conventionally reduced from the Average Daily Traffic (ADT). The other is driveway spacing, the length of the middle-lane section where two conflicting left-turn demands often compete for space. In addition, unlike previous researches, the way and the procedure the TWLTL operation is realized in the VISSIM S/W with its add-on application such as VISVAP is clearly stated and described in detail. According to the result of simulations for 10 volume scenarios, as expected, the higher the volume level is, the more delay the left-tuner experience. The Level Of Service (LOS) for most cases was in the range of C and D based on the non-signalized intersection LOS criteria. Furthermore, the TWLTL was found operable up to the volume level of 1,116 and 1,860 vph in heavy direction (equivalent of volume level 7) for 3-lane and 5-lane facility respectively, which covers significant portion of existing two to four-lane highway volumes in Korea.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.14 no.1
• /
• pp.464-469
• /
• 2013

After city of Busan has been entered to the aging society in 2000, the city has the highest aging rate among 7 representative cities in 2011. Moreover, while entire population and number of average household are decreasing, over 65 years old of elderly population is rapidly increasing. So, it is possible to enter the super-aged society, where aging rate would be about 20% after 2020. The purpose of this study is that older housing-related analysis is consisted of dong-unit, and this led microscopic analysis has become necessary. Surveys from 2000 through 2010, census aggregate (output area) unit of spatial analysis was conducted. Take advantages of this, aging population and area, soaring area, high-density areas, such as the region of interest were primary extracted, and microscopic location and spatial distribution patterns were analyzed. Upon analysis, aging population is concentrated in the city and adjacent area, the highlands, and 10 years of increasing rate was more than 30 times in certain aggregate. Regarding the characteristic of these areas, the original city center, Busan, especially concentrated and intensified in aging population. Also, 2000 to 2010, the overall distribution pattern of Busan has identified aging population that is increasingly being distributed. This is the result, which is confronted with previous research result. Entering a super aged-society for the future is accordance with migration of social costs and improve the quality of life of elderly. And this could be the basic information to use the spatial dimension for the corresponding.