• Journal of the Korean Wood Science and Technology
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• v.21 no.1
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• pp.39-50
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• 1993

The purpose of this study was to describe the micromorphological changes in Korean red pine (Pinus densiflora S. et Z.) wood decayed by a major brown-rot fungus, Lentinus lepideus, using scanning electron microscope and transmission electron microscope. At the end of the 12-week exposure to the fungus in soil block procedure(ASTM 1971), test blocks sustained 5.02% weight loss. The formation of bore hole by hyphae and penetration of hyphae through bordered pit were not observed. Instead, fungal hyphae appeared to penetrate axially tracheid luminar from the the ray cells via cross field pits. Hyphae were mainly found in lignin rich cell corner regions of tracheids, and also extensive degradation of tracheid wall occurred in this region. Extensive degradation of $S_2$ layer occurred without noticeable alteration of the $S_3$ layer, but warty layer and compound middle lamella remained relatively intact. Localized erosion, the characteristic of white rot, was observed in some cell wall and wall components including lignin were found to be decomposed.

• Journal of Acupuncture Research
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• v.22 no.5
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• pp.49-55
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• 2005

Objectives : In order to ensure safe Herbal Acupuncture treatment, the quality of needle tip is much important. Therefore we investigated micromorphological characteristics of Herbal Acupuncture needle tip according to the number of injection. Methods : We have selected the 26 Gage syringes & 29 Gage syringes that using currently in Herbal Acupuncture treatment. And we evaluated the change of shape of Herbal Acupuncture needle tip after several times of injection by Inverted System Microscope(x 200). Results & Conclusion : We found at Herbal Acupuncture needle tips had several defects such as hooked, scuffed, lump and irregular, stubbed or malformed shape in spite of new syringes. And we found at Herbal Acupuncture needle tips had several defects that is harmful to body such as mild pain, possibility of damage of tissue after several times of injection. Therefore it is necessary to pay attention to use Herbal Acupuncture needle tips.

• Korean Journal of Soil Science and Fertilizer
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• v.48 no.3
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• pp.189-193
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• 2015

Bad drainage problems from paddy fields adjacent to roads are caused by higher constructed roads which change topography of paddy fields to concave topography and artificial pan to prevent road erosion when road constructions are occurred. This study investigated effects of topography changes on soils by road constructions. Soil samples were investigated by physico-chemical analyses and micromorphology analyses from representative soil profile of Sachon series and soil samples. The characteristics of Sachon series that were adjacent to roads were fewer redoximorphic features (RMF) and increase in grey layers than the original Sachon series. The characteristics of Yecheon Series were shown from Jeollanamdo - Suncheon > Chungchungnamdo - Cheonan > Gangwondo - Wonju. Mosaic speckles were shown from micromorphological analyses because of repeat of shrink and expansion by wetting and drying. The location of graying in soil clods were found from coarse stone blocks to fine stone blocks and color changes of composed particles were also found.

• Microbiology and Biotechnology Letters
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• v.16 no.2
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• pp.156-162
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• 1988

Optimal conditions for the formation and regeneration of protoplasts of the cellulolytic fungus Penicillium verruculosum were investigated. Among the various commercial cell wall lytic enzymes tested, 0.5%(w/ v) Novozym 234 was the most effective for protoplast formation. The highest yield of protoplast exceeding 4.5$\times$10$^6$/m$\ell$ obtained when 400mg of 20 hr-old mycelia was incubated with 0.5%(w/v) Novozym 234 at 3$0^{\circ}C$ for 1 hr. The best osmotic stabilizer for the isolation and re-generation of protoplasts was 0.7M sorbital (pH 5.6) and 0.6M MgSO$_4$(pH 5.6), respectively. When 0.6M MgSO$_4$was added as osmotic stabilizer to the complete medium, the maximum regeneration frequency obtained was 4.6-27.8%. Micromorphological change of giant protoplasts into hyphae was observed during incubation in the regeneration liquid medium.