• 제목/요약/키워드: microbial property

검색결과 132건 처리시간 0.022초

표면 개질을 통한 미생물합성 폴리에스테르의 효소분해속도 조절 (Control of Enzymatic Degradability of Microbial Polyester by Surface Modification)

  • 이원기
    • 한국환경과학회지
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    • 제11권12호
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    • pp.1315-1320
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    • 2002
  • Since the enzymatic degradation of microbial poly[(R)-3-hydroxybutyrate-co-3-hydroxyvalerate] (P(3HB-co-3HV)) initially occurs by a surface erosion process, a degradation behavior could be controlled by the change of surface property. In order to control the rate of enzymatic degradation, plasma gas discharge and blending techniques were used to modify the surface of microbial P(3HB-co-3HV). The surface hydrophobic property of P(3HB-co-3HV) film was introduced by CF$_3$H plasma exposure. Also, the addition of small amount of polystyrene as a non-degradable polymer with lower surface energy to P(3HB-co-3HV) has been studied. The enzymatic degradation was carried out at 37 $^{\circ}C$ in 0.1 M potassium phosphate buffer (pH 7.4) in the presence of an extracellular PHB depolymerase purified from Alcaligenes facalis T1. Both results showed the significant retardation of enzymatic erosion due to the hydrophobicity and the enzyme inactivity of the fluorinated- and PS-enriched surface layers.

Relationship between Chemical Property and Microbial Activity of Reclaimed Tidal Lands at Western Coast Area in Korea

  • Ko, Eun-Seong;Joung, Ji-An;Kim, Chang-Hwan;Lee, Su Hwan;Sa, Tongmin;Choi, Joon-Ho
    • 한국토양비료학회지
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    • 제47권4호
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    • pp.254-261
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    • 2014
  • The scientific information between microbial activities and chemical properties of reclaimed tidal soil is not enough to apply for reclamation projects. This study was conducted to investigate the relation between chemical properties and microbial activities of reclaimed tidal lands located at western coastal area (25 samples from Nampo, Ewon, Sukmoon and Shihwa sites). Most of the reclaimed soils showed chemical characteristics as salinity soil except Nampo site. The major component influenced the salinity of reclaimed soil was identified as a sodium from the relationship between EC and exchangeable cation. With an increase in EC of soil, the population of mesophilic bacteria decreases whereas halotolerant and halophilic bacteria increases. The population of mesophilic bacteria increased with an increase in both organic matter and dehydrogenase activity. However, the population of halotolerant and halophilic bacteria decreased with an increase in organic matter. Based on the relation between chemical property and microbial activity of reclaimed tidal soil, electrical conductivity and organic matter as chemical properties of soil, population of mesophilic bacteria, halotolerant and halophilic bacteria and dehydrogenase activity as microbial activities could be the major parameters for reclamation process.

Microbial Transglutaminase Improves the Property of Meat Protein and Sausage Texture Manufactured with Low-quality Pork Loins

  • Katayama, K.;Chin, K.B.;Yoshihara, S.;Muguruma, M.
    • Asian-Australasian Journal of Animal Sciences
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    • 제19권1호
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    • pp.102-108
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    • 2006
  • Microbial transglutaminase (MTGase) was investigated to determine whether it was an effective binding agent for the processing of low-quality pork loins. MTGase especially promoted the coagulation of myosin heavy chain (MHC). However, the effect of MTGase on MHC from low-quality meat was less than that from the normal meat when the reaction time was not enough. The breaking strength of the heat-induced gel made of myosin B from low-quality meat with MTGase was lower than that of normal meat. Sausage made with low-quality meat with MTGase did not exhibit improved hardness, as compared to that made with normal meat. Results of this study indicated that use of low-quality meat in the manufacture of sausage was feasible to get textural property equal to that of normal meat sausage, when a half or more of the raw material was normal meat and MTGase was used in the sausage.

플라즈마를 이용한 미생물합성 폴리에스테르의 표면개질과 효소분해성 (Surface Modification and Enzymatic Degradation of Microbial Polyesters by Plasma Treatments)

  • 김준;이원기;류진호;하창식
    • 접착 및 계면
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    • 제7권2호
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    • pp.19-25
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    • 2006
  • 미생물 합성 고분자인 poly(hydroxylalkanoate)s (PHAs)의 초기효소분해는 표면침식의 메커니즘으로 진행하므로 이들의 분해거동은 표면특성을 개질로서 조절할 수 있다. 본 연구에서는 효소분해속도를 조절하기 위하여 플라즈마 기법을 PHAs 표면특성의 개질에 적용하였다. $CF_3H$$O_2$ 플라즈마를 사용하여 재료 표면에 각각 소수성 및 친수성을 부여하였다. 효소분해 실험은 pH 7.4의 0.1 M potassium phosphate 완충용액에서 Alcaligenes facalis T1에서 정제된 poly(hydroxybutyrate) 분해효소를 첨가하여 행하였다. $CF_3H$ 플라즈마 처리된 시편의 경우 표면 층의 불소화에 따른 소수성의 증가와 분해 효소에 대한 비활성으로 초기분해 속도가 상당히 지연됨을 관찰하였으나 $O_2$ 플라즈마 처리에 의한 표면 친수성은 분해속도의 촉진 등에 큰 영향을 미치지 않았다.

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미생물 처리 방법이 바이오그라우트에 미치는 영향 (Effect of Microbial Treatment Methods on Biogrout)

  • 김대현;박경호;김호철;이용희
    • 한국지반환경공학회 논문집
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    • 제13권5호
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    • pp.51-57
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    • 2012
  • 본 연구에서는 친환경적인 그라우트재의 개발을 위하여 연약지반에 대한 바이오그라우트 가능성을 확인하고, Bacillus Pasteurii 균을 이용하여 탄산칼슘 침전 효과를 분석하였다. 연약지반에 미생물의 탄산칼슘 침전을 이용하여 바이오그라우트에 미치는 영향을 알아보기 위해 4가지 시료의 조건(멸균 시료, 비멸균 시료, 반응용액과 미생물용액의 선처리 혼합시료, 반응용액과 미생물용액의 후처리 혼합시료)으로 실험되었다. 전자현미경(SEM), EDX와 X선 분석 회절기(XRD)를 이용하여 연약지반 시료의 분석을 수행하였고, 이러한 연구결과를 바탕으로 탄산칼슘 침전을 이용한 미생물 처리 공법은 바이오그라우트의 특성을 개선하였다.

栽培地域 差異에 따른 微생物 발효퇴비의 施용效果 (Effect of Microbial Fermentation Compost by Cultivating Area)

  • 이종성;정원복;정순재;오주성;강경희;김회태
    • 한국유기농업학회지
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    • 제10권1호
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    • pp.87-97
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    • 2002
  • 本 試驗은 정해지역의 慣行栽培 土壤과 부산지역의 신개간지 土壤에 微生物 발효퇴비를 각각 90kg/10a, 180kg/10a, 270kg/10a을 施用하여 土壤 微生物相 變化와 알타리 무의 生育, 收量 및 體內咸分에 미치는 影響을 究明하고자 試驗한 結果는 다음과 같다. 1. 栽培地域을 달리하여 미생물상를 調査하였던 바 신개간지 土壤에서 微生物數가 많았으며, 處理間 微生物數 變化는 化學肥料 處理區에서는 微生物數가 약간 減少하는 傾向을 보였으나 微生物 발효퇴비 處理區에서는 施用量이 增加할수록 綱菌數 및 放線菌數는 增加한 반면 絲狀菌數는 약간 減少하였다. 2. 栽培地域을 달리하여 알타리무를 栽培한 結果 김해지역의 慣行栽培 土壤에서 栽培한 알타리 무가 生育 및 收量이 높았으며, 微生物 발효비료 施用量에 따른 알타리 무의 生育 및 收量은 微生物 戀靜繼肥 施用量이 增加할수록 높았고, 특히 10a3당 270kg 處理區에서 가장 높았다. 3. 알타리무를 體內咸分을 분석한 結果 栽培地域 및 施用量間 뚜렷한 差異는 나타나지 않았다.

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멜라민 수지와 항균제 혼합 수지 가공 직물의 항균성과 피부 적합성 (Skin Patch Test and Antibacterial Properties of the Anti-microbial Agent and Melamine Resin Blend Treated Fabric)

  • 천태일;박정환
    • 한국의류산업학회지
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    • 제7권1호
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    • pp.81-84
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    • 2005
  • Skin patch test and antibacterial properties of the reaction products between poly(hexamethyl biguanide) hydrochloride and trimethylol melamine on textile fabrics were examined. Antibacterial activities of anti-microbial agent treated samples are very good. The reduction ratios against four kinds of colonies are 99.9 % after repeated laundering ten times. Skin patch test results for anti-microbial agent treated samples are almost-negative by Hi-scope judgement and macroscopical judgement.

Effectiveness of external agents in polluted sedimentary area

  • Alam, Md. Mahabub;Haque, Md. Niamul;Cho, Daechul;Kwon, Sung-Hyun
    • Environmental Engineering Research
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    • 제21권1호
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    • pp.52-57
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    • 2016
  • Sediment is a useful natural source but deteriorated continually by anthropogenic and industrial sources. Therefore, it is imperative to search a suitable method for improving or restoring sediment quality. Sediment has been tested to identify the effects of some external agents on a polluted area for 28 days. Chemical analysis and total viable counts (TVC) test have been conducted for 4 days interval to assess their performance. The analyses of chemical oxygen demand (COD), acid volatile sulfide (AVS), total phosphorous (T-P), total nitrogen (T-N) indicate that the chemical agents was more efficient to improve sediment quality whereas the microbial agent was more efficient for nutrient releasing from sediment. Oxygen releasing property of the chemical agent was thought to be providing with more congenial environment for the higher growth of the bacterial community than the direct application of microbial agents.

전기장하 토양내에서 미생물 이동특성과 동전기 생물학적복원의 효과 (Bacterial Behavior in Soil under Electric Field and its Effect on Electrokientic Bioremediation)

  • 김상준;박지연;이유진;양지원
    • KSBB Journal
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    • 제21권3호
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    • pp.175-180
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    • 2006
  • 전기장하 토양 내에서 미생물 이동은 주로 전기영동과 전기삼투에 의해 일어나며 미생물의 이동속도와 유속에 대한 전기영동의 공헌도가 전기삼투보다 대체로 높게 나타났다. Pentadecane-오염토양에 대해 동전기 생물학적복원을 실시한 결과 토양내 미생물 농도는 전기영동과 전기삼투가 함께 작용하여 양극과 음극의 인접 토양에서 동시에 증가하였으며 초반 공정이후에는 미생물의 표면전하특성과 양극의 산소발생에 의하여 미생물 농도가 양극, 중간, 음극의 순서로 나타났다. 하지만 미생물의 양방향 이동으로 토양의 모든 위치에서 오염물이 균일하게 제거될 수 있었다. 미생물의 전기적 이동을 이용한 동전기 생물학적복원은 기존의 생물학적복원의 단점인 늦은 분해속도와 낮은 제거효율의 단점을 극복할 수 있었다.

유기농 자재의 연용이 토양의 이화학적 성질과 토마토의 생육, 수량 및 체내성분에 미치는 영향 (Effect of Continuous Application of Organic Farming Materials on the Soil Physicochemistry Property and Plant Growth, Yield and Components of Tomato)

  • 오주성;이종성;강경희;김회태;정원복;정순재
    • 한국유기농업학회지
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    • 제9권1호
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    • pp.75-89
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    • 2001
  • This study the effects of the application of organic farming materials on the soil Physicochemistry property and plant growth, yield and components of Tomato were compared with conventional culture. The results obtained from the experiment are summarized as follows : 1. The effect of soil chemical properties after application organic farming materials, The amounts of pH and O.M. in N, P, K treated plot were few of change, To the contrary, fermentation compost and microorganisms fermentation compost treated plot were some increase, Amount of $P_2$$O_{5}$, Ca and K increase in comparison with the N, P, K treated plot. 2. Changes of soil microbial after application organic farming materials, The number of bacteria, actinomycetes and fungi in N, P, K treated plot were appeared definite direction. to the contrary, The number of bacteria and actimycetes in fermentation compost and microorganisms fermentation compost treated plot showed the increased tendency, The number of fungi showed the decreased tendency. 3. Effect of organic farming materials application on the growth and yield of tomato was superior in order of microorganisms fermentation compost plot〉 chemical fertilizers plot〉 fermentation compost plot. especially, chicken manure + microorganisms fermentation compost treated plot was the highest. 4. Effect of organic farming materials application on the components of tomato were not different.

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