• Proceedings of the Korean Society of Food and Cookery Science Conference
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• 2003.10a
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• pp.74-74
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• 2003

We investigated the microbial populations and viable cell counts of Codonopsis lanceolata from uncultivated and cultivated soil in the suing. The microbial populations and viable cell counts from both types of soils were also investigated simultaneously. It had existed 10 more kinds of microorganisms in uncultivated than those in cultivated. The total viable cell counts of C. laneolata from uncultivated soil, especially in the upper zone, were 9.7$\times$10$^{6}$ CFU/g. However, the C. lanceolata from cultivated soil was 4.2$\times$10$^{6}$ CFU/g. (omitted)

• Food Science and Preservation
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• v.6 no.1
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• pp.16-22
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• 1999

Microbial populations (total bacteria, lactic acid bacteria and coliforms), TBA, VBN and POV were investigated for evaluating the shelf life of pork loins gamma-irradiated at doses of 1, 3, 5 and 10 kGy with air-contained and vacuum-packaged methods. The initial microbial populations decreased with gamma irradiation depending upon the dose and microorganisms in the vacuum-packaged samples were inhibited more than those in the air-contained samples. POV, TBA and VBN values were higher in the air-contained samples than in the vacuum-packaged samples. In conclusion, the combination of gamma-irradiation and vacuum-packaging could extend the shelf life of chilled pork loin.

• Journal of Dairy Science and Biotechnology
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• v.36 no.2
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• pp.125-131
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• 2018

The present study aimed to investigate the microbial diversity in Gouda cheese within the four months of ripening, via next-generation sequencing (NGS). Lactococcus (96.03%), and Leuconostoc (3.83%), used as starter cultures, constituted the majority of bacteria upon 454 pyrosequencing based on 16S rDNA sequences. However, no drastic differences were observed among other populations between the center and the surface portions of Gouda cheese during ripening. Although the proportion of subdominant species was <1%, slight differences in bacterial populations were observed in both the center and the surface portions. Taken together, our results suggest that environmental and processing variables of cheese manufacturing including pasteurization, starter, ripening conditions are important factors influencing the bacterial diversity in cheese and they can be used to alter nutrient profiles and metabolism and the flavor during ripening.

• Horticultural Science & Technology
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• v.32 no.6
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• pp.901-905
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• 2014

The efficacy of ozone micro-bubble water (OMBW) in reducing microbial populations on alfalfa seeds was investigated in this study. We observed the surface of alfalfa seeds using microscopy and found that many cracks and crevices existing on the surface could harbor pathogens. Alfalfa seeds were treated with tap water (TW), micro-bubble water (MBW), ozone water (OW), ozone micro-bubble water (OMBW), and chlorine water (CL) for 5 min, and total microbial population, E. coli and Salmonella spp. colonies were determined. Also, the sterilized seeds were germinated and cultivated for 5 d after sowing to investigate the percentage of germination and the growth of alfalfa sprouts. The treatments with OMBW and CL were most effective in reducing total microbial populations and E. coli was eliminated by OW, OMBW, and CL treatments. CL treatment reduced the percentage of germination and fresh weight of alfalfa sprouts, but OMBW did not cause any negative effects on the germination and growth of alfalfa sprouts. These results indicate that OMBW can be used as an effective sanitizer for eliminating seed-borne pathogens without detrimental effects on seed viability.

• Molecular & Cellular Toxicology
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• v.2 no.1
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• pp.11-14
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• 2006

In this study we attempted to develop a novel genomic method to selectively isolate target functional microbial genomes from environmental samples. For this purpose, stable isotope probing (SIP) was applied in selectively isolating organic pollutant-assimilating populations. When soil microbes were fed with $^{13}C-labeled $ biphenyl, biphenyl-utilizing cells were incorporated with the heavy carbon isotope. The heavy DNA portion was successfully separated by CsCl equilibrium density gradient. And the diversity in the heavy DNA was sufficiently reduced, being suitable for the current DNA microarray techniques to detect biphenyl-utilizing populations in the soil. In addition, we proposed a new way to get more genetic information by combining this SIP method with selective metagenomic approach. The increased selective power of these new DNA isolation methods will be expected to provide a good quality of new genetic information, which, in turn, will result in development of a variety of biomarkers that may be used in assessing ecotoxicology issues including the impacts of organic hazards, and antibiotic-resistant pathogens on human and ecological systems.

• Korean Journal of Organic Agriculture
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• v.9 no.3
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• pp.103-117
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• 2001

This study was inducted to investigate the effects of microbial fertilizers on the fields of young radish(Raphanus sativus L.), chemical components of plant and soil, and the microbial floras. Six microbial fertilizers, MPK+Husk+Palma, Husk+Palma, MPK+Compost, Compost, BIO Livestock Clean System(BLCS) cattle dropping, and Tomi were used. The yields of young radish were increased in six microbial fertilizer treatments. The fresh matter weight, the number of leaves, and the dry matter weight of young radish in BLCS cattle dropping treatment, the leaf length in MPK+Husk+Palma treatment, the sugar content in Husk+Palma treatment, arid the leaf width in Tomi treatment showed the highest amount, respectively. The effects of microbial fertilizers on chemical characteristics of young radish and soil were examined. Phosphoric acid amount of young radish in Tomi treatment was much higher than other treatments. Potassium amount of young radish showed high significance in all microbial fertilizer treatments compared with control, and shoved the highest in Compost treatment . Two components, phosphoric acid find potassium, in soil inoculated by microbial fertilizers showed significant. Phosphoric acid in the Tomi treatment and Potassium in Husk+Palma treatment were increased. The microorganic populations in soil inoculated with microbial fertilizers were examined. While the number of Bacillus in ceil was increased in MPK+Husk+Palma treatment, the numbers of total bacteria, actinomycetes, and fungi were increased in Tomi treatment.

• Journal of Microbiology and Biotechnology
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• v.17 no.4
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• pp.560-570
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• 2007

The effective and accurate assessment of the total microbial community diversity is one of the primary challenges in modem microbial ecology, especially for the detection and characterization of unculturable populations and populations with a low abundance. Accordingly, this study was undertaken to investigate the diversity of the microbial community during the biodegradation of cis- and trans-dichloroethenes in soil and wastewater enrichment cultures. Community profiling using PCR targeting the l6S rRNA gene and denaturing gradient gel electrophoresis (PCR-DGGE) revealed an alteration in the bacterial community profiles with time. Exposure to cis- and trans-dichloroethenes led to the disappearance of certain genospecies that were initially observed in the untreated samples. A cluster analysis of the bacterial DGGE community profiles at various sampling times during the degradation process indicated that the community profile became stable after day 10 of the enrichment. DNA sequencing and phylogenetic analysis of selected DGGE bands revealed that the genera Acinetobacter, Pseudomonas, Bacillus, Comamonas, and Arthrobacter, plus several other important uncultured bacterial phylotypes, dominated the enrichment cultures. Thus, the identified dominant phylotypes may play an important role in the degradation of cis- and trans-dichloroethenes.

• Asian-Australasian Journal of Animal Sciences
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• v.26 no.1
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• pp.72-81
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• 2013

Four Luxi beef cattle ($400{\pm}10$ kg) fitted with ruminal, duodenal and ileal cannulas were used in a $4{\times}4$ Latin square to assess the effects of soybean small peptide (SSP) infusion on rumen fermentation, diet digestion and flow of nutrient in the gastrointestinal tract. The ruminal infusion of SSP was 0 (control), 100, 200 and 300 g/d. Ruminal SSP infusion linearly (p<0.01) and quadratically (p<0.01) increased microbial protein synthesis and rumen ammonia-N concentration. Concentrations of total volatile fatty acid were linearly increased (p = 0.029) by infusion SSP. Rumen samples were obtained for analysis of microbial ecology by real-time PCR. Populations of rumen Butyrivibrio fibrisolvens, Streptococcus bovis, Ciliate protozoa, Ruminococcus flavefaciens, and Prevotella ruminicola were expressed as a proportion of total Rumen bacterial 16S ribosomal deoxyribonucleic acid (rDNA). Butyrivibrio fibrisolvens populations which related to total bacterial 16S rDNA were increased (p<0.05), while Streptococcus bovis populations were linearly (p = 0.049) and quadratically (p = 0.020) decreased by infusion of SSP. Apparent rumen digestibility of DM and NDF were (Q, p<0.05; L, p<0.05) increased with infusion SSP. Total tract digestion of DM, OM and NDF were linearly (p<0.01) and quadratically (p<0.01) increased by infusing SSP. The flow of total amino acids (AA), essential amino acids (EAA) and individual amino acids were linearly (p<0.01) and quadratically (p<0.01) increased with infusion SSP. The digestibility of Lysine was quadratically (p = 0.033) increased and apparent degradability of Arginine was linearly (p = 0.032) and quadratically (p = 0.042) increased with infusion SSP. The results indicated that infusion SSP could improve nutrient digestion, ruminal fermentation and AA availability.

• Journal of Forest and Environmental Science
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• v.30 no.4
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• pp.370-377
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• 2014

There are very few studies about soil chemical and biological properties under tropical dry evergreen forest Coromandel Coast, India. The present study was conducted in six tropical dry evergreen forests sites such as Oorani, Puthupet, Vadaagram, Kotthatai, Sendrakillai and Palvathunnan. We measured the quantity of soil chemical, biological properties and selected soil microorganisms for investigating the impacts of soil microbial populations on soil chemical and biological properties. The result showed that total N, P, Ca, S, Fe, Mn, Cu, Co, exchangeable K, Olson P, extractable Ca and phosphobacterial population were higher in the soil from Kothattai forest site. Organic carbon, total Mg, extractable Na, soil respiration, ${\beta}$-glucosidase activity, bacterial population, fungi population and actinomycetes population were higher in the soil from Palvathunn forest site. Total K, $NH_4{^+}$-N, $NO_3{^-}$-N, exchangeable K, extractable Ca, extractable Na, azotobacter population, bacillus population and rhizobacteria population were higher in the soil from Sendrakillai. Beijerinckia population, rhizobacteria and soluble sodium were higher in Puthupet forest soil. Total Si, total Na and exchangeable K were higher in soil from Oorani forest site. Total Mo and exchangeable K were higher in the soil from Vadaagaram forest site. The results showed that organic carbon, total N, $NH_4{^+}$-N, $NO_3{^-}$-N, extractable P, extractable Ca, soil respiration and ${\beta}$-glucosidase were significantly correlated with soil microbial populations. Therefore soil microorganisms are important factor for maintaining soil quality in tropical dry evergreen forest.

• Asian-Australasian Journal of Animal Sciences
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• v.14 no.12
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• pp.1747-1751
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• 2001

An experiment was conducted to determine the effects of dietary oligosaccharides on performance and intestinal microbial populations of piglets. Ten litters of piglets were assigned to five groups randomly, with two litters per group. The control group was fed with corn-soybean basal diet. Oligosaccharides was added to the basal diet at the level of 0.05%, 0.1%, 0.2% and 0.35% respectively to form four experimental diets. The experiment was conducted with two periods. The first period (suckling period) was from 7 to 28 days of age and the second period (weanling period) was from 28 to 56 days of age. Fresh fecal samples were collected at 21 days of age and assayed for Escherichia coli concentration, pH and moisture content. Three pigs per group were slaughtered at 42 days of age and cecum, colon, and rectum content samples were collected and assayed immediately for Escherichia coli and Bifidobacterium concentration, pH and moisture content. The results showed that dietary oligosacchrides decreased fecal Escherichia coli population and pH significantly (p 0.05), but did not affect performance and fecal moisture content during suckling period. 0.1% oligosaccharides for weanling pigs increased growth and improved feed conversion ratio together with a reduction of diarrhea (p 0.05), but 0.35% oligosaccharides did not affect growth performance. 0.1% and 0.2% oligosaccharides for weanling pigs had a suppression to Escherichia coli colonization in rectum and an enrichment to Bifidobacterium in colon (p 0.05). Oligosaccharides decreased significantly (p<0.05) rectum moisture content, but did not affect cecum, colon and rectum pH.