• KSBB Journal
• /
• v.18 no.3
• /
• pp.243-247
• /
• 2003

The microbial immobilization media with curdlan and activated carbon which has great immobilization capacity has been developed. Characteristics of porosity and mechanical strength of this support media are dependent on manufacturing method. The support media showed the best cell immobilization performance when the ratio of curdlan and activated carbon was 30 g/L to 6 g/L in this study. The immobilization of iron-oxidizing bacteria on the supporting particles was photographed with a scanning electron microscope. Since cell concentration on the surface of supporting particle increased with the reaction time, the iron oxidation rate also increased.

• Applied Biological Chemistry
• /
• v.43 no.3
• /
• pp.202-206
• /
• 2000

Feasibility of microbial culture media using by-product of salt fermented kanary was investigated. Gram negative strain, Escherichia coli, and Gram positive strain, Bacillus subtilis, and bioluminescent Photobacterium Phosphoreum were incubated with kanary by-Product media (KB media). Compared with LB media, KB media had enough carbon source, but lacked nitrogen source and growth factor. When 0.5% of peptone as a nitrogen source and 0.3% of yeast extract as nitrogen and growth factor source were fortified in KB media, the cell population rate was similar to LB media. Also, when 0.5% of yeast extract was fortified to KB media, it showed the same result as in LB media. The price of KB media with fortification of 0.5% peptone and 0.3% yeast extract, and 0.5% of yeast extract is only 46 and 19% of that of LB media, respectively. These results showed that kanary by-Product could be a good and cheaper bacterial culture media if small amount of nitrogen source and growth factor were added.

• Korean Journal of Environmental Biology
• /
• v.41 no.3
• /
• pp.308-324
• /
• 2023

This study investigated unrecorded freshwater bacterial species in Korea. Water and sediment samples were collected from the Nakdong River basin from 2020-2022. Bacterial isolates obtained through the conventional culture method with commercial media were subjected to 16S rRNA gene sequencing to identify unrecorded bacterial species. Results of 16S rRNA gene sequencing of the bacterial isolates revealed that a total of 44 bacterial isolates shared 16S rRNA gene sequence similarities of more than 98.65%, with validly published bacterial species not reported in Korea yet. These isolates were phylogenetically assigned to 4 phyla, 7 classes, 21 orders, 33 families, and 42 genera. A total of 2, 6, 12, and 24 species belonged to phyla Bacillota, Bacteroidota, Actinomycetota, and Pseudomonadota, respectively. Here, we provide details of these 44 unrecorded bacterial species, including Gram staining, colony and cellular morphologies, biochemical properties, and phylogenetic position.

• KSCE Journal of Civil and Environmental Engineering Research
• /
• v.9 no.2
• /
• pp.135-141
• /
• 1989

Blast furnace slag presents coarse surface for microbes to grow on and high calcium and magnesium contents to neutralize acid to be produced during anaerobic digestion. Also, slag contains aluminum and iron oxides which would promote biological flocculation, and minerals which would stimulate microbial growth. Acid wastes like dairy waste, carbohydrate waste, sanitary landfill leachate and molases wastes were applied without neutralization to laboratory reactors to examine the applicability of blast furnace slag as media. The study results indicated slag media was effective to neutralize pH and maintain microbial population in the system. Particularly, COD removal efficiency was greater than those from plastic media operations treating dairy waste at higher loading rates.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
• /
• 2002.04a
• /
• pp.319-322
• /
• 2002

The bacterial transport in soil media was studied. Nonionic surfactants, enhanced the bacterial transports in soil media. The transport rate in soil column was increased by increasing the number of ethylene oxide in polyoxyethylene oxide surfactants. Ionic strength of solution affected the microbial transport characteristics in soil. The hydrophobicity of cell surface was proved that one of important characteristics on the bacterial transport in soil media.

• Microbiology and Biotechnology Letters
• /
• v.48 no.3
• /
• pp.328-336
• /
• 2020

Parachlorella sp. is an efficient fatty acid producer that can be used in the production of biofuels, feeds, and fertilizers. Microalgae show varying responses to culture conditions, even those within the same species. In this study, growth and fatty acid composition of a newly isolated Parachlorella sp. from the Nakdong river of Korea in different culture media were investigated. The microalga was cultivated in 400 ml bubble column photobioreactors using BG-11, BBM, TAP, and modified TAP (MTAP) media. It was shown that using BBM led to greater fatty acid accumulation (34%), while using TAP medium led to greater biomass productivity (0.34 g/l/day). Composition of the TAP medium was modified to have the N:P ratio of BBM while also varying concentrations of N and P to improve fatty acid productivity. One of the modified TAP media, MTAP-1 (104.8 mgN/l, 135.2 mgP/l, N:P ratio = 0.77), showed the highest fatty acid concentration of 0.69 ± 0.04 g/l, while those from TAP and BBM were 0.48 ± 0.06 g/l and 0.40 ± 0.02 g/l, respectively. The results showed that microalgal fatty acid productivity could be enhanced by changing the N:P ratio and concentrations.

• Environmental Engineering Research
• /
• v.24 no.2
• /
• pp.181-190
• /
• 2019

Constructed wetlands are improvised man-made systems, designed for adopting the principle of natural wetlands for purifying wastewater - the elixir of life. They are used widely as a cost-effective and energy-efficient solution for treating greywater generated from different tertiary treatment sources. It provides an elaborate platform for research activities in an attempt to recycle earth's natural resources. Among the several organic impurities removal mechanisms existing in constructed wetland systems, the earth's active microbial population plays a vital role. This review deals with the recent advancements in constructed wetland systems from a microbiological perspective to (effect/ devise/ formulate) chemical and physical treatment for water impurities. It focuses on microbial diversity studies in constructed wetlands, influence of wetland media on microbial diversity and wetland performance, role of specific microbes in water reuse, removal of trace elements, some heavy metals and antibiotics in constructed wetlands. The impurities removal processes in constructed wetlands is achieved by combined interactive systems such as selected plant species, nature of substrate used for microbial diversity and several biogeochemical effected reaction cycles in wetland systems. Therefore, the correlation studies that have been conducted by earlier researchers in microbial diversity in wetlands are addressed herewith.

• Microbiology and Biotechnology Letters
• /
• v.21 no.4
• /
• pp.381-391
• /
• 1993

As the first stage of development of an artificial skin, fibroblasts were cultured in the collagen matrices to make a living dermal equivalent. Mouse embryonic fibroblasts were incorporated into a collagen matrices on plastic dishes containing concentrated DMEM culture media supplemented with sodium bicarbonate, hepes, antibiotics and fetal bovine serum. As the growth stimulation components, glycosaminoglycans were added: hyaluronic acid, chondroitin sulfate, heparin, chitosan were incorporated into the media at a concentration of either 1% or 5% w/w/ to collagen in order to investigate the effect on development of dermal equivalent. After the few days of incubation, gel matrics were contracted and firm dermal equivalent were formed. And the keratinocytes were cultured on top of dermal equivalent and make a three dimensional artificial skin tissue.

• Proceedings of the Korean Society for Agricultural Machinery Conference
• /
• 2001.02a
• /
• pp.387-392
• /
• 2001

• Journal of Food Hygiene and Safety
• /
• v.31 no.6
• /
• pp.465-470
• /
• 2016

Sample preparation methods were evaluated for effectiveness in detecting foodborne pathogens from sprout seeds. The methods included: Rinse.-Test portions were rinsed with 0.1% peptone water, and the pellet after centrifugation was inoculated into pre-enrichment media; and Sprouting.-Seed samples were sprouted before pre-enrichment and sprouted seeds were inoculated into pre-enrichment media. In rinse method, E. coli was isolated from 13 of 280 sample units. In sprouting method, E. coli was isolated from 12 of 135 sample units. E. coli O157:H7, Salmonella spp., and L. monocytogenes were not detected in any of the samples. In the trials for recovering Salmonella enterica from artificially contaminated alfalfa seeds, the soak, rinse, and sprouting methods were evaluated. The detection rates of S. enterica were statistically different according to the amount of the sample tested and selective medium type (P < 0.05).