• Title/Summary/Keyword: microbial load

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Statistical Analysis of Operating Parameters on Advanced Wastewater Treatment Plant (고도처리 하수처리장 운전조건의 통계분석)

  • Lee Chan-Hyung;Moon Kyung-Sook
    • Journal of Environmental Science International
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    • v.14 no.2
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    • pp.251-258
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    • 2005
  • Statistical analysis between operating parameters and effluent quality on advanced wastewater treatment plant was performed. Through factor analysis four factors derived varimax rotation were selected each plant. Four components explained $80\%,\;82\%$ of the total variance of the process, respectively. The components on MLE plant were identified in the following order: 1) HRT increase and BOD load decrease by influent decrease, 2) Biomass, 3) SVI increase by internal return increase, 4) Microbial diversity by SRT increase. On $A_2O$ plant, we defined them as follows: factor 1, high MLSS by return rate increase, HRT increase by influent decrease; factor 2, biomass; factor 3, BOD of influent; factor 4 was relate to DO.

Relationship of Oral Bacterial Load Over One Year of Smoking Cessation

  • Kim, Sunghyun;Seo, Min-Seock;Hwang, Soo-Jeong
    • Journal of dental hygiene science
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    • v.19 no.4
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    • pp.213-219
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    • 2019
  • Background: Smoking exerts an adverse effect on the periodontal tissue by reorganizing the ecosystem of oral microorganisms and is considered to be an important factor in the development of periodontal disease. Although cross-sectional studies on smokers and non-smokers have been attempted to investigate the microbial differences in periodontal oral cavity, only few studies have been conducted to investigate the changes in oral microorganisms during smoking cessation. The purpose of this study was to investigate the changes of bacteria in saliva and gingival crevicular fluid (GCF) over a period of one year among 11 smokers trying to quit smoking. Methods: Eleven smokers trying to quit smoking visited the clinic at baseline, two weeks, two months, four months, six months, and 12 months to give saliva and GCF samples. The amounts of 16S rRNA, Porphyromonas gingivalis, Treponema denticola, Prevotella intermedia, Fusobacterium nucleatum subsp. nucleatum, Streptococcus mutans, and Streptococcus sobrinus in saliva and GCF were quantified using real-time polymerase chain reaction TaqMan probe assay. The results were analyzed by nonparametric statistical analysis using Friedman test and Spearman correlation coefficient. Results: After cessation of smoking, the amounts of 16S rRNA corresponding to P. gingivalis, F. nucleatum, P. intermedia, and T. denticola in saliva decreased and then again increased significantly. The amount of F. nucleatum 16S rRNA in GCF decreased significantly after smoking cessation. Positive correlations were observed between 16S rRNA and F. nucleatum and between F. nucleatum and T. denticola in saliva and GCF. Conclusion: Even if the number of subjects in this study was small, we suggest that smoking cessation may reduce the total bacterial amount and F. nucleatum in GCF. However, the results regarding changes in the microbial ecosystem due to smoking or smoking cessation were inconsistent. Therefore, further in-depth studies need to be carried out.

Effect of Chlorine Dioxide Gas Application to Egg Surface: Microbial Reduction Effect, Quality of Eggs, and Hatchability

  • Chung, Hansung;Kim, Hyobi;Myeong, Donghoon;Kim, Seongjoon;Choe, Nong-Hoon
    • Food Science of Animal Resources
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    • v.38 no.3
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    • pp.487-497
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    • 2018
  • Controlling of microorganisms in the industrial process is important for production and distribution of hatching and table eggs. In the previous study, we reported that chlorine dioxide ($ClO_2$) gas of a proper concentration and humidity can significantly reduce the load of Salmonella spp. on eggshells. In this study, we compared microbial reduction efficacy on egg's surface using hatching eggs and table eggs, internal quality of table eggs, and hatchability after both the conventional method (washing and UV expose, fumigation with formalin) and $ClO_2$ gas disinfection. Application of 40 ppm $ClO_2$ gas to the table and hatching eggs, respectively, reduced the aerobic plate count (APC) with no statistical difference compared with the conventional methods. Additionally, we didn't observed that any significant difference in albumin height, Haugh unit (HU), and yolk color, this result confirms that 40 ppm $ClO_2$ had no effect on the internal quality of the table eggs, when comparing with the UV treatment method. The hatchability of hatching eggs was not statistical different between formaldehyde fumigation and 80 ppm $ClO_2$ gas treatment, though the value was decreased at high concentration of 160 ppm $ClO_2$ gas. From these results, we recommend that $ClO_2$ gas can be used as a safe disinfectant to effectively control egg surface microorganisms without affecting egg quality.

Kimchi Quality Affected by the Addition of Grapefruit Seed Extract Powder (자몽종자추출물 분말제재를 첨가한 김치의 저장성 연장)

  • 박우포;장덕규
    • Food Science and Preservation
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    • v.10 no.3
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    • pp.288-292
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    • 2003
  • Grapefruit seed extract powder (GFSEP), which was made with grapefruit seed extract and calcium carbonate, was investigated to retard the fermentation rate. The mixing ratios of GFSEP to salted Chinese cabbage were 0.1, 0.3 and 0.5%. Quality characteristics of kimchi such as pH, titratable acidity, reducing sugar content and microbial loads were measured during fermentation at 10$^{\circ}C$. pH and reducing sugar content of control and kimchi sample with 0.1% GFSEP showed decreases until 10 days, and then attained to stabilized levels. Kimchi samples with 0.3% and 0.5% GFSEP maintained a higher pH and reducing sugar content, while microbial load was lower than others. Based on the pH and tit ratable acidity, kimchi samples with GFSEP prolong the shelf life about 3~10 days.

The Quality Characteristics of Kimchi as Affected by the Addition of Xanthan Gum (증점안정제로 사용한 Xanthan Gum첨가가 김치의 품질에 미치는 영향)

  • Park, Woo-Po
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.31 no.3
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    • pp.423-427
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    • 2002
  • Xanthan gum (XG) was investigated for use as a thickening and stabilizing agent in kimchi during fermentation at 10$\^{C}$. The mixing ratios of XG to salted Chinese cabbage were 0.1, 0.3 and 0.5%. Quality characteristics of kimchi such as pH, titratable acidity, reducing sugar content and microbial loads were measured. pH and reducing sugar content showed abrupt decreases after 5 day lag time until 20 days, while titratable acidity steadily increased during fermentation. The addition with 0.5% XG retarded the change rates of pH and titratable acidity showing the slowest change. Kimchi samples added with 0.1% and 0.3% XG maintained a higher reducing sugar content during the whole fermentation period of 30 days. Microbial loads showed an abrupt increase from 5 to 10 days, and maintained a nearly same load thereafter. Kimchi sample added with 0.3% XG showed better scores in color, aroma and taste.

Effect of Water Temperature and Packing Type on Quality of Fresh-cut Chicory (세척수 온도와 포장 형태에 따른 신선편이 치커리의 품질 변화)

  • Chang, Min-Sun;Kim, Gun-Hee;Kim, Byeong-Sam
    • Journal of Food Hygiene and Safety
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    • v.22 no.4
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    • pp.279-287
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    • 2007
  • Quality attributes of fresh-cut chicory (Clchorium intybus L.var. foliosum) treated with hydrocooling and packing type were investigated in terms of weight loss, respiration, vitamin C content, microbial load and sensory properties during storage at $4^{\circ}C\;and\;10^{\circ}C$. Fresh chicory was trimmed and washed 3 times with cold water $(1^{\circ}C,\;5^{\circ}C)$ and tap water $(10^{\circ}C)$ for 30 sec and then packaged in polypropylene (PP) film bag and polyethylene terephthalate (PETE) tray, and stored for 9 days at $4^{\circ}C\;and\;10^{\circ}C$. Weight loss was decreased by washing and packing generally. Respiration rate was increased slowly in the storage at $4^{\circ}C$. Vitamin C content of chicory packaged within PETE tray were decreased gradually during storage at $4^{\circ}C$. Hydrocooling and packing within PETE tray treatments resulted in approximately 1-2 log CFU/g reduction of microbial load.

Effect of Water Temperature and Packing Type on Quality of Fresh-Cut Sesame Leaf (세척 깻잎의 선도 유지에 대한 세척수 온도와 포장 형태의 영향)

  • Kim, Byeong-Sam;Chang, Min-Sun;Park, Shin-Young;Cha, Hwan-Soo;Kwon, Ki-Hyun
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.37 no.2
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    • pp.231-238
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    • 2008
  • Quality attributes of fresh-cut sesame leaf (Perilla frutescens var. japonica) as affected by hydrocooling and packing were investigated in terms of weight loss, respiration, vitamin C content, total chlorophyll content, microbial load and sensory properties during storage at 4 and $10^{\circ}C$. Fresh sesame leaf was trimmed and washed with cold water (1, $5^{\circ}C$) as well as tap water ($13^{\circ}C$) for 30 sec 3 times and then packaged in PP (polypropylene) film bag and PETE (polyethylene terephthalate) tray, and stored for 9 days at 4 and $10^{\circ}C$. Weight loss was decreased by washing and packing. Respiration rate was increased slowly over the storage at $4^{\circ}C$. Vitamin C content and total chlorophyll contents of sesame leaf packaged within PETE tray decreased gradually during storage. Hydrocooling and packing within PETE tray treatments resulted in approximately $1{\sim}2\;log\;CFU/g$ reduction of microbial load.

Effect of Water Temperature and Packing Type on Quality of Fresh-cut Pak-choi (세척 청경채의 선도 유지에 대한 세척수 온도와 포장 형태의 영향)

  • Kim, Byeong-Sam;Chang, Min-Sun;Park, Shin-Young;Cha, Hwan-Soo;Kwon, Ki-Hyun;Kim, Gun-Hee
    • Food Science and Preservation
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    • v.15 no.1
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    • pp.1-8
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    • 2008
  • Quality attributes of fresh-cut pak-choi (Brassica campestris var. chinensis) as affected by hydrocooling and packing were investigated in terms of weight loss, respiration, vitamin C content, total chlorophyll content, microbial load and sensory properties during storage at 4 and $10^{\circ}C$. Fresh pak-choi was trimmed and washed with cold water $(1^{\circ}C)$ as well as tap water $(6^{\circ}C)$ for 30 sec 3 times and then packaged in PP (polypropylene) film bag and PETE (polyethylene terephthalate) bay, and stored for 9 days at 4 and $10^{\circ}C$. Weight loss was decreased by washing and packing generally. Respiration rate was increased slowly over the storage at $4^{\circ}C$. Vitamin C content and total chlorophyll contents of pak-choi packaged within PETE bay decreased gradually during storage. Hydrocooling and packing within PETE bay treatments resulted in approximately 1-2 log CFU/g reduction of microbial load.

Quality Comparison between Gamma Irradiation and Freeze Drying Methods in Preparing Kimchi for the Long-term Storage (장기저장이 가능한 김치제조를 위한 감마선 조사와 동결건조법의 품질비교)

  • Park, Jin-Gyu;Park, Jae-Nam;Han, In-Jun;Song, Beom-Seok;Kim, Jae-Hun;Lee, Ju-Woon;Hwang, Han-Joon;Kim, Young-Duk;Byun, Myung-Woo
    • Food Science and Preservation
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    • v.15 no.1
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    • pp.9-14
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    • 2008
  • This study compared microbial load, texturre, histological properties, and sensory characteristics of gamma-irradiated (20 kGy) Kimchi and freeze-dried Kimchi under condition; of long-tenn storage. immediately after irradiation, no aerobic bacteria were detected, but microbial load of freeze-dried Kimchi decreased by only 1 log cycle in comparision with the nonirradiated control sample. The firmness of irradiated Kimchi was significantly higher than that of rehydrated freeze-dried Kimchi (p<0.05). Scanning electron microscopy showed collapse of the microstructure in epidermis tissues of rehydrated Kimchi. 'The sensory scores for color, texture, and taste of irradiated Kimchi were higher than those for rehydrated freeze-dried Kimchi.

Effects of Washing-water Temperature and Packaging Type on the Quality of Fresh-cut Crown Daisies (세척 쑥갓의 선도 유지에 미치는 세척수 온도와 포장 형태의 영향)

  • Chang, Min-Sun;Kim, Byeong-Sam;Kim, Gun-Hee
    • Korean Journal of Food Science and Technology
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    • v.40 no.2
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    • pp.171-177
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    • 2008
  • The effects of hydrocooling and packaging type on the quality attributes of fresh-cut crown daisies (Chrysanthemum coronarium var. spatiosum) were investigated by examining weight loss, respiration, vitamin C content, total chlorophyll content, microbial load, and sensory properties during storage at 4 and 10$^{\circ}C$. Fresh crown daisies were trimmed and washed with cold water (1 and 5$^{\circ}C$) as well as tap water (10$^{\circ}C$) 3 times each for 30 sec. They were then packaged in PP (polypropylene) film bags or PETE (polyethylene terephthalate) trays, and stored for 9 days at 4 and 10$^{\circ}C$, respectively. In general, weight loss was reduced as a result of the washing and packaging. The respiration rate increased slowly during storage at 4$^{\circ}C$, and the vitamin C and total chlorophyll contents of the crown daisies packaged in PETE trays decreased gradually during storage. Finally, the treatments consisting of hydrocooling and then packaging in PETE trays resulted in approximately 1-2 log CFU/g reductions in microbial load.