• Title/Summary/Keyword: microbial degradation,

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Microbial Degradation of Non-volatile-amine (미생물에 의한 불휘발성아민의 분해)

  • LEE Tae-Seek;PARK Jeong-Heum;LEE Myung-Suk;HUR Sung-Ho
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.23 no.1
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    • pp.1-6
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    • 1990
  • The degradation of non-volatile-amines by microorganisms were investigated. The degra-ding activity could be noted in four strains isolated from fermented sardine sauce, and those were Pseudomonas aeruginosa, Pseudomonas fluorescens P-2, Pseudomonas fluorescens P-3 and Enterobacter aerogenes. The strongest degrading activity of non-volatile-amines was showed in Pseudomonas fluorescens P-3 among the four strains isolated. The optimum temperature for degradation by Pseudomonas fluorescens P-3 was $35^{\circ}C$, corresponding to the optimum temperature for growth of this strain, pH between 7.0 and 7.5 could gave effective degradation and the optimum concentration of NaCl was 0 and/or $1\%$.

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Enhanced Natural Purification of Crude Oil Contaminated Tidal Flat (원유로 오염된 갯벌 지역의 자연정화 기능 향상 기술의 개발)

  • Kim, Young-A;Sung, Ki-June
    • Journal of Soil and Groundwater Environment
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    • v.16 no.5
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    • pp.24-30
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    • 2011
  • Tidal flats which are ecologically sensitive, are hard to remediate once they are contaminated by oil spill accidents. Traditional oil remediation measures focus on removal efficiency, and their improper implementation can adversely affect crude oil contaminated coastal areas and greatly disrupt the structure and functions of crude oil contaminated tidal flats. In this study, the oil degradation due to the implementation of remediation measures naturally enhanced using air and natural oil sorbents was evaluated in the lower strata of tidal flats. The effects of air and natural oil sorbents on oil degradation for two concentration levels (< 500 ppm and > 500 ppm) were tested at artificially contaminated tidal flats. Fifty days after these treatments, the natural oil sorbent treatment showed the lowest total petroleum hydrocarbon (TPH) concentration ($4.46{\pm}1.47%$) at the low concentration level, whereas both air and natural oil sorbent treatments showed high degradation efficiencies at the high concentration level ($29.30{\pm}4.39%$). Although the phosphatase activity decreased for all treatments, there was no significant difference between the decreases for the different treatments; on the other hand, B-glucosidase activities were high for both air and natural oil sorbent treatments. Although degradation efficiencies decreased as the concentration increased, the air provision and natural oil sorbent treatment could be an effective ecological restoration measure for oil contaminated tidal flats while minimizing the environmental impact of the remediation efforts.

Bacterial Degradation of Monoethanolamine (생물학적 방법에 의한 Monoethanolamine의 분해 연구)

  • Hyun, Jun-Taek;Rhee, In-Hyoung;Kwon, Sung-Hyun;Kim, Dong-Jin;Cho, Dae-Chul
    • KSBB Journal
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    • v.22 no.3
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    • pp.157-161
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    • 2007
  • This study is to investigate the biological degradation and the characteristics of MEA, a pH regulator to be put in the cooling water circulation system for power plants, loading to elevate concentrations of COD and N when eluted into the water environment. MEA, $NH_4^+$ and CODcr were monitored in flask cultures and in a batch aerator. MEA was found to be biologically degradable, producing substantial amount of ammonia (max. 78.1%) in a form of $NH_4^+$ and other carboneous intermediates. The degradation reaction rates were similar one another over all MEA concentrations tested as the activated sludge (microbial consortium) was acclimated to MEA with the gradual and stepwise increase in MEA input into the batch aerator. Also, MLVSS kept increasing with increasing MEA input. The COD-based degradation reaction order was determined to be 1.

Effect of Repeated Application of IBP on the Degradation of Pesticides in Flooded Soil (IBP의 반복처리(反復處理)가 담수토양중(湛水土壤中) 농약(農藥)의 분해(分解)에 미치는 영향(影響))

  • Song, Byeong-Hun;Jeong, Young-Ho;Park, Young-Sun
    • Korean Journal of Environmental Agriculture
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    • v.1 no.1
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    • pp.65-70
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    • 1982
  • This experiment was conducted to see the effect of repeated application of IBP granular formulation(17%, 0,0-diisopropyl-S-benzyl thiophosphate) on the biodegradation of IBP and diazinon〔0,0-diethyl 0-(2-isopropyl-4-methyl-5-pyrimidinyl) phosphorothioate〕 in silt loam soil with 2.1% organic matter under flooded condition. The persistence of IBP in the soil was shortened by increasing the frequencies of application of the chemical. Enhanced degradation ability in the soil caused by repeated application of IBP was prolonged about 53 days, while the ability did not influence diazinon persistence in the soil. The half-lives of IBP in sterilized soil autoclaved at $121^{\circ}C$ for 30 minutes were about 3 times longer than those in viable soil, suggesting that microbial process was a major factor for IBP degradation in the soil. The total colony number of soil microbes showed little difference between the soils with and without repeated application of IBP. A possible concern of specific soil microorganisms on the pesticide degradation in soil was discussed.

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Biodegradation of JP-8 in soil column by Rhodococcus fascians isolated from petroleum contaminated soil (유류 오염 토양에서 분리된 Rhodococcus fascians 를 이용한 토양 column에서의 JP-8의 분해)

  • Park, Bong-Je;Noh, Yong-Ho;Yun, Hyun-Shik
    • KSBB Journal
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    • v.23 no.6
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    • pp.479-483
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    • 2008
  • The environmental contamination by organic pollutants is a widespread problem. The most widely distributed pollution can be attributed to oil contamination. Bioremediation, the use of microorganism or microbial processes to degrade environmental contaminant, is one of the new technologies. The objective of the present study is to study the degradation of JP-8 in soil by microorganism. The degradation of JP-8 was analysed by TPH using gas chromatography. Rhodococcus fascians isolated from the petroleum contaminated site was applied for the degradation of JP-8 in the soil column system. Air flow rate of 30 ml/min was sufficient to degrade JP-8 in the soil column as much as 70% of JP-8 in the soil column. The addition of nitrogen source resulted in the increase in JP-8 degradability to 75% of JP-8 and the C:N ratio for JP-8 degradation was 100:10.

Degradation of Diazinon and Dursban in Submerged Soil (담수양중(湛水壤中) Diazinon 과 Dursban 의 분해(分解)에 관(關)하여)

  • Choi, Jong-Woo;Lee, Kyu-Seung
    • Korean Journal of Environmental Agriculture
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    • v.6 no.2
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    • pp.1-11
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    • 1987
  • The degradation of two chemicals seem to be clearly affected by soil microbial activity in submerged soil $conditions(30{\pm}1^{\circ}C)$. The Active ingredient of Diazinon disappeared about 5 times faster than that of Dursban. By Applying 300% higher concentrations of both chemicals. under the above soil conditions, however, degradation was retarded by about one day. Some of the metabolites of Diazinon were as follows: 0.0-diethyl phosphorothioate and sulfotep as hydrolytic products, and diazoxon, 0.0-diethyl-0-[2-(1-hydroxy-1, 1-dimethyl)-6-methyl]-pyrimidinyl phosphorothioate and 2-isopropyl-6-methyl-pyrimidine-4-one as degradation products of monooxygenase. But 0. 0-diethyl phosphorothioate was the only methabolite of Dursban.

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Microbial degradation and other methods for accelerated degradation the Herbicide Imazapyr (제초제 Imazapyr 의 미생물에 의한 분해 및 기타 방법에 의한 분해 촉진)

  • Lee, Jae-Koo;Kwon, Jeong-Wook
    • Korean Journal of Environmental Agriculture
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    • v.17 no.1
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    • pp.5-10
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    • 1998
  • The microbial degradation, photosensitizer-mediated photolysis, and bioceramic- accelerated degradation of the herbicide imazapyr were investigated using four types of soil. 1. Seven strains of microorganisms isolated from the soil A and the active sludge collected from the waste water disposal plant in CheongJu did not give any distinct degradation products in pure culture. When imazapyr (10ppm) was incubated for 14days with each of the 6strains of the known bacteria, they did not produce any noticeable products, either, suggesting that imazapyr was degraded very little by microorganisms in aqueous media. Meanwhile, when 50ppm of imazapyr was incubated in soil A and B for 6months, a degradation product of m/z 279 was detected. It turned out to be 2-[(1-carbamoyl-1,2-dimethylpropyl)carbamoyl]nicotinic acid, which was formed by the hydrolytic cleavage of the imidazolinone ring and by tautomerism. When imazapyr was exposed to sunlight, degradation rates were 14.6% under the control and 66.0, 76.5, 26.7, and 90.0% in the presence of PS-1 (100ppm), PS-1 (200ppm), PS-2(100ppm), and PS-3(100ppm), respectively, and a degradation product of m/z 149 was tentatively identified in the treatment of PS-1. 2. When soil C and D treated with bioceramic were incubated for 7weeks, the $^{14}C$-activities of $^{14}CO_2$ evolved were 2.03 and 1.12% of the originally applied ones, respectively, whereas those in control soils without bioceramic were 1.88 and 0.82% showing no significant defferences.After 5 weeks, however,the differences in the amounts of $^{14}CO_2$ between the two treatments increased gradually, suggesting the bioceramic effect.

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Sustainability Indices (=Green Star) for Microbial Fuel Cell (미생물 연료전지 영속발전 지표개발)

  • Song, Ha-Geun;KOO, Ja-Kong
    • Journal of the Korea Organic Resources Recycling Association
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    • v.23 no.2
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    • pp.47-52
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    • 2015
  • A microbial fuel cell (MFC) is a device that can be obtained electricity from a variety of organic through the catalytic reaction of the microorganism. The MFC can be applied to various fields, and research is required to promote the performance of the microbial fuel cell for commercialization. The lower performance of an MFC is due to oxygen reduction at the cathode and the longer time of microbial degradation at anode. The MFC amount of power is sufficient but, in consideration of many factors, as a renewable energy, now commonly power density as compared to Nafion117 it is an ion exchange membrane used is PP (Poly Propylene) from 80 to about 11 fold higher, while reducing the cost to process wastewater is changed to a microporous non-woven fabric of a low cost, it may be energy-friendly environment to generate electricity. All waste, in that it can act as a bait for microorganisms, sustainability of the microbial fuel cell is limitless. The latest research on the optimization and performance of the operating parameters are surveyed and through the SSaM-GG(Smart, Shared, and Mutual- Green Growth) or GG-SSaM(Green Growth - Smart, Shared, and Mutual) as the concept of sustainable development in MFC, the middle indices are developed in this study.

Protein Evaluation of Dry Roasted Whole Faba Bean (Vicia faba) and Lupin Seeds (Lupinus albus) by the New Dutch Protein Evaluation System: the DVE/OEB System

  • Yu, P.;Egan, A.R.;Leury, B.J.
    • Asian-Australasian Journal of Animal Sciences
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    • v.12 no.6
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    • pp.871-880
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    • 1999
  • The effects of dry roasting (110, 130, $150^{\circ}C$ for 15, 30, 45 min) on potential ruminant protein nutritional values in terms of: a), rumen bypass protein (BCP); b), rumen bypass starch (BST); c), fermented organic matter (FOM); d), true absorbed bypass protein (ABCP); e) microbial protein synthesized in the rumen based on available energy (E_MP); f), microbial protein synthesized in the rumen based on available nitrogen (N_MP); g), true protein supplied to the small intestine (TPSI); h), true absorbed rumen synthesized microbial protein (AMP); i), endogenous protein losses (ENDP); j), true digested protein in the small intestine (DVE); k), degraded protein balance (OEB) of whole lupin seeds (WLS) and faba beans (WFB) were evaluated by the new Dutch DV/OEB protein evaluation system. Dry roasting significantly increased BCP, BST, TPSI, ABCP, DVE (p<0.001) and decreased FOM, E_MP, AMP, N_MP and OEB (p<0.001) with increasing temperatures and times except that when temperature was at $110^{\circ}C$. The values of BCP, BST, TPSI, ABCP and DVE at $150^{\circ}C/45min$ for WLS and WFB were increased 2.2, 3.7; -, 2.0; 1.7, 1.7; 2.3, 3.7 and 1.7, 1.7 times and the values of FOM, E_MP, AMP, N_MP and OEB at $150^{\circ}C/45min$ for WLS and WFB were decreased by 15.3, 25.8; 18.1, 25.8; 18.7, 25.8; 54.6, 41.6 and 82.3% 54.7%, respectively, over the raw WLS and WFB. The results indicated that though dry roasting reduced microbial protein synthesis due to reducing FOM, TPSI didn't decrease but highly increased due to increasing BCP more than enough for compensation of the microbial protein decreasing. Therefore the net absorbable DVE in the small intestine was highly increased. The OEB values were significantly reduced for both WLS and WFB but not to the level of negative. It indicated that microbial protein synthesis might not be impaired due to the sufficient N supplied in the rumen, but the high positive OEB values in the most treatments except of $150^{\circ}C$ for 30 and 45 min of WLS (The OEB values: 54.8 and 26.0 g/kg DM) indicated that there were the large amounts of N loss in the rumen. It was concluded that dry roasting at high temperature was effective in shifting protein degradation from rumen to intestines and it increased the DVE values without reaching the negative OEB values. No optimal treatment was found in WLS due to the too high OEB values in all treatments. But dry roasting at $150^{\circ}C$ for 30 and 45 min might be optimal treatments for WLS due to the very lower OEB values.

Thermophilic Biofiltration of Benzene and Toluene

  • Cho, Kyung-Suk;Yoo, Sun-Kyung;Ryu, Hee-Wook
    • Journal of Microbiology and Biotechnology
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    • v.17 no.12
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    • pp.1976-1982
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    • 2007
  • In the current studies, we characterized the degradation of a hot mixture of benzene and toluene (BT) gases by a thermophilic biofilter using polyurethane as a packing material and high-temperature compost as a microbial source. We also examined the effect of supplementing the biofilter with yeast extract (YE). We found that YE substantially enhanced microbial activity in the thermophilic biofilter. The degrading activity of the biofilter supplied with YE was stable during long-term operation (approximately 100 d) without accumulating excess biomass. The maximum elimination capacity ($1,650\;g{\cdot} m^{-3}{\cdot} h^{-1}$) in the biofilter supplemented with YE was 3.5 times higher than that in the biofilter without YE ($470\;g{\cdot} m^{-3}{\cdot} h^{-1}$). At similar retention times, the capacity to eliminate BT for the YE-supplemented biofilter was higher than for previously reported mesophilic biofilters. Thus, thermophilic biofiltration can be used to degrade hydrophobic compounds such as a BT mixture. Finally, 168 rDNA polymerase chain reaction-DGGE (PCR-DGGE) fingerprinting revealed that the thermophilic bacteria in the biofilter included Rubrobacter sp. and Mycobacterium sp.