• Journal of Ginseng Research
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• 제39권4호
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• pp.392-397
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• 2015

Background: Red ginseng (RG) is processed from Panax ginseng via several methods including heat treatment, mild acid hydrolysis, and microbial conversion to transform the major ginsenosides into minor ginsenosides, which have greater pharmaceutical activities. During the fermentation process using microbial strains in a machine for making red ginseng, a change of composition occurs after heating. Therefore, we confirmed that fermentation had occurred using only microbial strains and evaluated the changes in the ginsenosides and their chemical composition. Methods: To confirm the fermentation by microbial strains, the fermented red ginseng was made with microbial strains (w-FRG) or without microbial strains (n-FRG), and the fermentation process was performed to tertiary fermentation. The changes in the ginsenoside composition of the self-manufactured FRG using the machine were evaluated using HPLC, and the 20 ginsenosides were analyzed. Additionally, we investigated changes of the reducing sugar and polyphenol contents during fermentation process. Results: In the fermentation process, ginsenosides Re, Rg1, and Rb1 decreased but ginsenosides Rh1, F2, Rg3, and Compound Y (C.Y) increased in primary FRG more than in the raw ginseng and RG. The content of phenolic compounds was high in FRG and the highest in the tertiary w-FRG. Moreover, the reducing sugar content was approximately three times higher in the tertiary w-FRG than in the other n-FRG. Conclusion: As the results indicate, we confirmed the changes in the ginsenoside content and the role of microbial strains in the fermentation process.

• 한국수소및신에너지학회논문집
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• 제28권5호
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• pp.440-446
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• 2017

This study was aimed at evaluating the effects of carbohydrate, protein and lipid content of substrate on hydrogen yields and microbial communities. The hydrogen yields were linearly correlated to carbohydrate content of substrates while others (content of proteins and lipids) did not make a significant contribution. The chemical composition of substrates produced effects on the final products of anaerobic hydrogen fermentation. Acetate and butyrate were the main fermentation products, with their concentration proving to correlate with carbohydrate and protein content of substrates. The result of microbial community analysis revealed that the relative abundances of Clostridium butyricum increased and Clostridium perfringens decreased as the carbohydrate content increased.

• 대한한의학회지
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• 제32권5호
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• pp.41-49
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• 2011

Objectives: To optimize the preservation method of herbal decoction, we investigated the content of principle components of Pyungwi-san, liquiritin, glycyrrhizin, and hesperidin according to preservation temperature and period. We also investigated the changing patterns of pH and microbial population in Pyungwi-san decoction as a model case. Methods: With samples preserved at different temperatures, the content of liquiritin, glycyrrhizin, and hesperidin was determined using HPLC and microbial population was determined as viable counting method up to 8 times every month. Identification of isolated bacteria was performed by 16S rDNA analysis. Results: The content of liquiritin and glycyrrhizin did not change according to the preservation temperature and period, but that of hesperidin was severely decreased at room temperature. The isolate from the decoction was identified as Bacillus licheniformis by 16S rDNA sequence analysis. Microbial population appeared after 3 months' preservation and reached maximum value at 4 months; at all tested temperatures, the pH showed the lowest value (4.4-4.5) simultaneously. Conclusion: From the results, it seems to be that the microbial growth affects the pH of preserved decoction but not the change of liquiritin and glycyrrhizin content.

• The Korean Journal of Ecology
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• 제9권1호
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• pp.9-18
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• 1986

The decomposition of litters of Pinus densiflora and the growth of microbial populations in a calcareous region, Youngwal were compared with those in a noncalcareous region, Sinlim. The decay rate of litter in Pinus densiflora in Youngwal was 0.128 and that in Sinlim was 0.096. The differences in the populations of soil bacteria and total microorganisms between the two regions were signficant at the 5% level, but that of fungi and actinomycetes was not at that level. The differences in the content of calcium and pH value of soil between the two regions were significant at the 1% level. The excessive content of calcium became to increase pH value, in turn the high pH decreased the content of available phosphorus in soil. The vertical distribution of the content of available phsophorus was consistent with that of the populations of fungi and actinomycetes in Youngwal.

• Asian-Australasian Journal of Animal Sciences
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• 제9권6호
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• pp.655-665
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• 1996

The effects of feeding rice bran and beet pulp mixtures on the site and extent of digestion and microbial synthesis in fattening steers were studied. Three Holstein steers fitted with ruminal, duodenal and ileal cannulas were fed four diets in a $4{\times}3$ Youden square design. The four diets consisted of 15% Italian ryegrass hay and 85% concentrate as a control diet which included 72% rolled barley, 20% rice bran plus 40% beet pulp, 30% rice bran plus 30% beet pulp or 40% rice bran plus 20% beet pulp. All diets provided 1.8 times digestible energy required for maintenance. The digestibility of fat in the small intestine (% of flow) showed an increase with rice bran content among the by-product diets. Digestibility of structural carbohydrate both in the rumen and the whole digestive tract decreased linearly with rice bran content. The digestibility of nonstructural carbohydrate was not affected by rice bran content, but that of nonstructural, nonstarch polysaccharides was higher in the rumen and lower in the large intestine for the by-product diets than for the control diet. A rice bran content of more than 30% in the by-product diets severely inhibits ruminal microbial synthesis and digestible energy intake in fattening steers.

• 한국토양비료학회지
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• 제44권5호
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• pp.696-701
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• 2011

전북지역 과수원 토양 110개소를 대상으로 토양특성이 미생물분포에 미치는 영향을 조사하였다. 토양 중 미사함량이 많아질수록 호기성세균, 곰팡이, 미생물 biomass C 함량은 증가하였고, 탈수소효소 활성은 토성에 따라 차이가 없었다. 지형에 따른 미생물분포, 미생물의 biomass C 함량, 탈수소효소 활성은 차이가 없었고, 과수종류에 따라 coliform group은 배와 포도 재배지에서 각각 $133.0{\times}10^3\;CFU\;g^{-1}$, $107.4{\times}10^3\;CFU\;g^{-1}$으로 가장 높은 수준을 보였다. 과수재배기간이 길어질수록 미생물 밀도는 감소하는 것으로 나타났다. 토양 pH, 유기물, 치환성 Mg 함량은 모든 미생물분포와 정의 상관관계를 보였고, Bacillus sp.는 조사한 모든 화학성분과 정의 관계를 보였다. 토양미생물의 biomass C 함량과 탈수소효소 활성은 유기물과 치환성 Ca 함량에 대해 고도의 유의성 (p<0.01)을 보였다.

• 유기물자원화
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• 제26권4호
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• pp.31-43
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• 2018

본 연구는 유기 농경지의 토양 및 양분관리 방법으로 가축분과 풋거름(헤어리베치)공급, 윤작 시스템. 그리고 경운 및 무경운 처리가 토양환경에 미치는 영향을 구명하고자 수행하였다. 신규 옥수수 재배 토양의 양분공급원으로 가축분 퇴비, 헤어리베치와 같은 유기물 공급이 화학비료 처리에 비해 토양 유기물 함량을 높이며 토양의 미생물 밀도와 미생물체량 C함량을 증가시키는 것을 알 수 있었다. 또한 토양 미생물 군집의 기능적 다양성이 증가하는 것을 확인하였다. 윤작재배 효과로 토양 화학성은 처리간의 차이가 뚜렷하게 나타나지 않았지만 옥수수-밀, 옥수수-헤어리베치 윤작 처리구가 옥수수 단작 처리구보다 토양의 미생물체량 C 함량과 종 다양성 지수가 높은 경향을 보였다. 경운 여부에 따른 토양 화학성은 처리간의 뚜렷한 차이를 나타내지 않았다. 토양 미생물 군집의 기질 이용에서 무경운이 경운처리 보다 다소 높게 나타났지만 통계적인 유의차는 없었다. 처리에 따른 토양의 화학성과 미생물상간의 요인간 상관관계 분석 결과, 토양 유기물 함량과 치환성 칼륨 함량은 기질 이용도, 기질 풍부도와 양의 상관관계를 보였다. 이러한 결과로부터 가축분 퇴비, 헤어리베치와 같은 유기물 시용으로 옥수수 재배 토양의 화학성 및 미생물상에 긍정적인 효과를 나타낸 것으로 판단되었다.

• Asian-Australasian Journal of Animal Sciences
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• 제14권1호
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• pp.54-60
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• 2001

A study was conducted to assess the nutritive value of two diets based on a low-energy variety of wheat, RAC C1 and their effects on intestinal mucosal structure and function in broiler chickens. The diets were fed with or without microbial enzyme supplement to male and female broiler chickens. The digesta viscosity was reduced (p<0.001) through supplementation with a microbial enzyme in male and female chicks. Enzyme supplementation also improved the dietary apparent metabolizable energy content (p<0.001) and had slight but non-significant positive effects on chick growth and feed conversion ratio. Intestinal mucosal structure and enzyme function were not affected by microbial enzyme supplement. Male chicks consumed more feeds (p<0.001), attained higher final body weight (p<0.001) and were more efficient at feed utilization (p<0.01) than the female chicks. Except for duodenal villus surface area and ileal protein content, intestinal mucosal structure and enzyme activities were similar between the two sexes and dietary treatment groups. The study showed an improvement in the nutritive value of the diets in the presence of the microbial enzyme supplement.

• 한국의류산업학회지
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• 제22권4호
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• pp.523-533
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• 2020

Previous studies are used to examine cellulose content, degumming period, fiber quality, production yield, production cost, development limit of fiber according to physical, chemical, and microbial degumming methods. Three types of degumming methods are used to measure surface condition after degumming, necessity of additional degumming and degree of impurity removal. First, previous studies confirmed that the microbial degumming method is superior in terms of cellulose content, fiber quality, production yield, production cost, and fiber development possibility. Second, surface condition and the necessity of additional degumming were analyzed by SEM. The black skin binding material was removed in the case of the Sangnangyi and chemical degumming; however, it was insufficient and further degumming was required. Skin fiber binding material was removed in the case of microbial degumming and the surface was cleanest after degumming; in addition, most showed the form of yarn decomposition. The FT-IR spectrum determined the degree of removal of impurities and showed that it can utilize inherent physical properties as the best degumming method. The degree of removal of pectin and lignin by microbial degumming was cleanest with hemicellulose also reduced by microbial degumming.

• 환경위생공학
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• 제14권1호
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• pp.24-30
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• 1999

A biological filter for treatment of toluene among volatile organic compounds was studied. The investigation was conducted using specially built stainless steel columns packed with granular activated carbon and cold for removal of toluene. The G.A. and mold as filter material was also coated with Pseudomonas putida microorganisms.The biofilter unit was operated in the condition of moisture content vairation at gas loading rate of 12.5 l/min. Gaseous toluene taken from tedlar bag was analyzed by the use of G.C equipped with F.I.d detector. The removal efficiency of gaseous toluene was 95% at average inlet concentration of 950 ppm during bio-degradation operating condition. Effective removal efficiency was obtained with moisture content 27.5% at activated carbon and 32% at mold in this study. The effective operating condition were obtained with pH 6-8, temperature 28-42℃ for microbial degradation at gas loading rate of 12.5 l/min in packed material.