• 설비공학논문집
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• 제20권4호
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• pp.252-259
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• 2008

The purpose of this study is to estimate the movement of microbial contaminant caused by bio-attack using bio-agent such as bacillus anthracis for preventing contaminant diffusion. multizone simulation was carried out in the case of three types of bio-attack scenario in the government building. Simulation results show that severe contaminant diffusion is brought about in all cases of bio-attack scenario in one hour, though pollution boundaries have different mode according to bio-attack scenarios. Simulation results also show that immune building technology such as filter and UVGI technology gives us powerful alternatives to meet the emergent situation caused by unexpected bio-attack.

• 설비공학논문집
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• 제19권4호
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• pp.291-298
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• 2007

The purpose of this study is to evaluate the efficacy of multizone simulation that enables to grasp of details about microbial contaminant problem in an multistoried apartment. We used actual indoor test data to figure up microbial contaminant level as initial value for the multizone simulation and estimated the various effects of indoor occupant infected with germs such as bacteria and fungus and the performance of air sterilization by using multizone simulation in substitute for infeasible experimental approach. The results show that natural ventilation make ourselves generally useful for removing indoor microbial contaminants. The results also show that the performance of air sterilization reach the maximum in the case of using mechanical ventilation and UVGI air sterilizer. The conclusion is that this multizone simulation is useful tool for actual design method for immune building systems.

• 한국물환경학회지
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• 제22권3호
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• pp.504-512
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• 2006

In order to estimate microbial contaminant discharge from diffuse sources, rainfall runoff was monitored at forestry, agriculture and urban watersheds. Total coliform and E. coli were monitored at the study watersheds as they are regulated by the environmental laws. Concentration and EMC (Event Mean Concentration) of coliform of rainfall runoff at the urban watershed were the highest followed by those from agricultural and forestry watersheds. By monitoring coliform concentrations of overlying water and sediment at five monitoring points in the downstream of the Geum River, average concentration from spring to summer was higher than those values from fall to spring. Coliform concentrations in the pore water were higher compared to those of overlying water and closely related with flow rate of the river.

• 대한설비공학회:학술대회논문집
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• 대한설비공학회 2006년도 하계학술발표대회 논문집
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• pp.319-324
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• 2006

The purpose of this study Is to evaluate the efficacy of multizone simulation that enables to grasp of details about microbial contaminant problem in an multistoried apartment. We used actual indoor test data to figure up microbial contaminant level as initial value for the multizone simulation and estimated the various effects of indoor occupant infected with germs and the performance of air sterilization by using multizone simulation in substitute for infeasible experimental approach. The results show that natural ventilation make ourselves generally useful for removing indoor microbial contaminants. The results also show that the performance of air sterilization reach the maximum in the case of using mechanical ventilation and UVGI air sterilizer. The conclusion is that this multizone simulation is useful tool for actual design method for Immune building systems.

• 한국지하수토양환경학회:학술대회논문집
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• 한국지하수토양환경학회 2002년도 총회 및 춘계학술발표회
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• pp.18-21
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• 2002

Phytoremediation which uses plants to enhance the bioremediation through stimulation of microbial activity and root uptake, has been a topic of increasing interest. Mathematical model were developed that can be applied to various bioremediation methods in the unsaturated zone, especially phytoremediation, for simulating the fate and transport of contaminants under field conditions. A 2-year field study was conducted using 72 (1.5m long and 0.1 m diameter) column lysimeters with four treatments: Johnsongrass; wild rye grass; a rotation of Johnsongrass and wild rye grass; and unplanted fallow conditions. The developed model represented the fate and transport of contaminant both in vegetated and unplanted soils satisfactorily for field applications. Parameters related to the contaminant concentration in the water phase were the main parameters determining the contaminant fate in the vadose zone and indicated that the bioavailability can be the most important factor in the success of phytoremediation as well as bioremediation applications.

• 대한설비공학회:학술대회논문집
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• 대한설비공학회 2007년도 하계학술발표대회 논문집
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• pp.137-137
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• 2007

• 한국미생물학회:학술대회논문집
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• 한국미생물학회 2008년도 International Meeting of the Microbiological Society of Korea
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• pp.86-88
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• 2008

• KSBB Journal
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• 제21권3호
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• pp.175-180
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• 2006

전기장하 토양 내에서 미생물 이동은 주로 전기영동과 전기삼투에 의해 일어나며 미생물의 이동속도와 유속에 대한 전기영동의 공헌도가 전기삼투보다 대체로 높게 나타났다. Pentadecane-오염토양에 대해 동전기 생물학적복원을 실시한 결과 토양내 미생물 농도는 전기영동과 전기삼투가 함께 작용하여 양극과 음극의 인접 토양에서 동시에 증가하였으며 초반 공정이후에는 미생물의 표면전하특성과 양극의 산소발생에 의하여 미생물 농도가 양극, 중간, 음극의 순서로 나타났다. 하지만 미생물의 양방향 이동으로 토양의 모든 위치에서 오염물이 균일하게 제거될 수 있었다. 미생물의 전기적 이동을 이용한 동전기 생물학적복원은 기존의 생물학적복원의 단점인 늦은 분해속도와 낮은 제거효율의 단점을 극복할 수 있었다.

• Journal of Microbiology and Biotechnology
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• 제23권5호
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• pp.674-680
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• 2013

Yeast Dekkera/Brettanomyces bruxellensis is probably the most common contaminant in wineries and ethanol production processes. The considerable economic losses caused by this yeast, but also its ability to produce and tolerate high ethanol concentrations, make it an attractive subject for research with potential for industrial applications. Unfortunately, efforts to understand the biology of D. bruxellensis and facilitate its broader use in industry are hampered by the lack of adequate procedures for delivery of exogenous DNA into this organism. Here we describe the development of transformation protocols (spheroplast transformation, LiAc/PEG method, and electroporation) and report the first genetic transformation of yeast D. bruxellensis. A linear heterologous DNA fragment carrying the kanMX4 sequence was used for transformation, which allowed transformants to be selected on plates containing geneticin. We found the spheroplast transformation method using 1M sorbitol as osmotic stabilizer to be inappropriate because sorbitol strikingly decreases the plating efficiency of both D. bruxellensis spheroplast and intact cells. However, we managed to modify the LiAc/PEG transformation method and electroporation to accommodate D. bruxellensis transformation, achieving efficiencies of 0.6-16 and 10-20 transformants/${\mu}g$ DNA, respectively. The stability of the transformants ranged from 93.6% to 100%. All putative transformants were analyzed by Southern blot using the kanMX4 sequence as a hybridization probe, which confirmed that the transforming DNA fragment had integrated into the genome. The results of the molecular analysis were consistent with the expected illegitimate integration of a heterologous transforming fragment.

• 한국지하수토양환경학회지:지하수토양환경
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• 제20권4호
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• pp.15-21
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• 2015

Anaerobic biodegradation of diesel with coexisting heavy metals (Pb) was monitored in batch mode. Two different groups of the indigenous bacteria from a site contaminated with diesel and lead were used in this research: the first group was composed of a single species and the second group was composed of several species. The effect of heavy metals on the microbial population was monitored and confirmed the biodegradation mechanism in each combined contaminant. Growth of the microorganisms in 21 days was observed Diesel > Diesel + Pb > Diesel + Cu > Diesel + Pb + Cu > Diesel + Cr > Diesel + Pb + Cr. Indigenous microorganisms showed the adaptation in the Pb contaminate. Interactive toxic effect using AMES test observed larger synergistic effect than antagonistic in Diesel + Cr and Diesel + Pb + Cr. Therefore, the main effects of diesel biodegradation in the present of heavy metals are likely to exist other factors as well as toxic of heavy metals. This is a necessary part of the future studies.