• Title/Summary/Keyword: microbial composition

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Effects of Dietary Antimicrobial Agents, Probiotics or Yucca Extract on Urease Activity and Ammonia Production in the Chicken Intestine (사료중 항균제, 생균제 또는 유카 추출물이 닭의 장내 요소 분해효소 활성과 암모니아 생산에 미치는 영향)

  • 김규일;여진모
    • Korean Journal of Poultry Science
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    • v.22 no.2
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    • pp.105-115
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    • 1995
  • The balance of microbial populations in the gastrointestinal (GI) tract of all warm-blooded animals is critical to the maintenance of health and resistance to disease. The composition of the populations can be altered by diet and environment, making the host animal susceptible to disease, and reducing growth rate and feed efficiency. Some feed additives including antimicrobial agents, prohiotics or yucca extract have been used to promote growth and feed utilization. There is evidence that part of growth-promoting effect of those feed additives results from the suppression of microbial urease activity or ammonia production in the GI contents of animals. Over 200 microbial species have been known to produce urease and the product of urea hydrolysis, ammonia, is toxic to animals. Carefully tested probiotics or other urease-suppressing agents can be a possible alternative to antimicrobial agents including antibiotics as growth promotants used for animals feeds.

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비친수성유기물질(HOC)로 오염된 토양의 정화를 위한 동전기-생물활성화공정의 개발

  • 양지원;김상준;박지연;이유진;기대정
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 2003.09a
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    • pp.326-329
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    • 2003
  • When an electrokinetic process is applied to a HOC-contaminated soil, hybrid types combined with soil flushing, chemical oxidation, and bioremediation are generally used. Especially when the electrokinetic process is combined with bioremediation, the hybrid technology can solve several limits of bioremediation such as low microbial mobility, low soil temperature, and shortage of nutrients in subsurface circumstance. Because microbial surface is charged negatively, the microorganism moves from cathode to anode under electrical field. In this study, mixed culture mainly-consisted by Pseudomonas sp. was applied to remediate pentadecane-contaminated kaolinite with particle size less than 300${\mu}{\textrm}{m}$. This remediation system was named ‘electrokinetic bioaugmentation’ and consisted of model aquifer, electrode reservoirs, bioreactor, power supply, and pump. The mixed culture above 0.5 of optical density in bioreactor was supplied to two reservoirs and penetrated soil when the electric current was applied. To enhance the removal efficiency, the optimal medium composition, electric current, and voltage were investigated.

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Effects of Carbohydrate, Protein and Lipid Content of Substrate on Hydrogen Production and Microbial Communities (탄수화물, 단백질, 지방 함량에 따른 혐기성 수소 발효시 부산물 및 미생물 군집 특성 평가)

  • LEE, CHAE-YOUNG;HAN, SUN-KEE
    • Transactions of the Korean hydrogen and new energy society
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    • v.28 no.5
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    • pp.440-446
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    • 2017
  • This study was aimed at evaluating the effects of carbohydrate, protein and lipid content of substrate on hydrogen yields and microbial communities. The hydrogen yields were linearly correlated to carbohydrate content of substrates while others (content of proteins and lipids) did not make a significant contribution. The chemical composition of substrates produced effects on the final products of anaerobic hydrogen fermentation. Acetate and butyrate were the main fermentation products, with their concentration proving to correlate with carbohydrate and protein content of substrates. The result of microbial community analysis revealed that the relative abundances of Clostridium butyricum increased and Clostridium perfringens decreased as the carbohydrate content increased.

An Integrated Database and Web Service for Microbial Resources at KACC

  • Kim, Chang-Kug;Jeon, Young-Ah;Cho, Gyu-Taek;Kwon, Soon-Wo;Kim, Yong-Hwan;Hong, Seung-Beom
    • Genomics & Informatics
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    • v.7 no.1
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    • pp.41-45
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    • 2009
  • The Korean Agricultural Culture Collection (KACC) has developed a web-based system to provide an integrated database with information updates about microbial resources. This integrated database consists of 5 major functions and contains general information, which includes identification numbers, culture media composition, image information, DNA sequences, patent information, and general forms for ordering and depositing microorganisms. In 2008, KACC started providing characterization information. KACC maintains 9,801 cultures of microorganisms, including 3,296 strains of bacteria, 4,734 fungi, 784 actinomycetes, 64 yeasts, and 923 others.

Effect of Cathodic Biofilm on the Performance of Air-Cathode Single Chamber Microbial Fuel Cells

  • Ahmed, Jalal;Kim, Sung-Hyun
    • Bulletin of the Korean Chemical Society
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    • v.32 no.10
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    • pp.3726-3729
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    • 2011
  • Biofilm formation is inevitable in a bioelectrochemical system in which microorganisms act as a sole biocatalyst. Cathodic biofilm (CBF) works as a double-edged sword in the performance of the air-cathode microbial fuel cells (MFCs). Proton and oxygen crossover through the CBF are limited by the robust structure of extracellular polymeric substances, composition of available constituents and environmental condition from which the biofilm is formed. The MFC performance in terms of power, current and coulombic efficiency is influenced by the nature and origin of CBF. Development of CBF from different ecological environment while keeping the same anode inoculums, contributes additional charge transfer resistance to the total internal resistance, with increase in coulombic efficiency at the expense of power reduction. This study demonstrates that MFC operation conditions need to be optimized on the choice of initial inoculum medium that leads to the biofilm formation on the air cathode.

Roads to Construct and Re-build Plant Microbiota Community

  • Kim, Da-Ran;Kwak, Youn-Sig
    • The Plant Pathology Journal
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    • v.38 no.5
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    • pp.425-431
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    • 2022
  • Plant microbiota has influenced plant growth and physiology significantly. Plant and plant-associated microbes have flexible interactions that respond to changes in environmental conditions. These interactions can be adjusted to suit the requirements of the microbial community or the host physiology. In addition, it can be modified to suit microbiota structure or fixed by the host condition. However, no technology is realized yet to control mechanically manipulated plant microbiota structure. Here, we review step-by-step plant-associated microbial partnership from plant growth-promoting rhizobacteria to the microbiota structural modulation. Glutamic acid enriched the population of Streptomyces, a specific taxon in anthosphere microbiota community. Additionally, the population density of the microbes in the rhizosphere was also a positive response to glutamic acid treatment. Although many types of research are conducted on the structural revealing of plant microbiota, these concepts need to be further understood as to how the plant microbiota clusters are controlled or modulated at the community level. This review suggests that the intrinsic level of glutamic acid in planta is associated with the microbiota composition that the external supply of the biostimulant can modulate.

Changes of Soil Microbial Phospholipid Fatty Acids as Affected by Red Pepper Cultivation and Compost Amendment (고추재배지에서 퇴비시용에 따른 토양 미생물의 인지질지방산 변화)

  • Park, Kee-Choon;Kim, Su-Jung
    • Korean Journal of Soil Science and Fertilizer
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    • v.43 no.2
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    • pp.194-199
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    • 2010
  • Compost as a soil amendment is of importance in enhancing the soil chemical and microbial qualities; however, soil microbial community can vary depending on the composition, and the amount of compost applied to plant in the soil. Responses of soil microbial properties to compost applications with 0, 30, and 60 Mg $ha^{-1}$ were investigated in silt loam soils where red pepper(Capsicum annuum L.) was mainly cultivated in Yeongyang, Gyeongbuk, Korea. The analysis of phospholipid fatty acids (PLFAs) extracted from soil showed that compost amounts significantly increased PLFAs representing as bacteria, fungi, and VAM-fungi as well as the ratio of fungi/bacteria, and monounsaturated/saturated PLFAs. Increasing the amount of compost significantly increased Gram-/Gram+ PLFAs' ratio, but significantly decreased monounsaturated/saturated PLFAs' ratio. Therefore, this result shows that compost would vary to a limited extent the microbial community in red pepper field. However, increase in compost application would change the subgroup structure of microbial community only.

Direct Monitoring of Membrane Fatty Acid Changes and Effects on the Isoleucine/Valine Pathways in an ndgR Deletion Mutant of Streptomyces coelicolor

  • Tae-Rim Choi;Suk Jin Oh;Jeong Hyeon Hwang;Hyun Jin Kim;Nara Shin;Jeonghee Yun;Sang-Ho Lee;Shashi Kant Bhatia;Yung-Hun Yang
    • Journal of Microbiology and Biotechnology
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    • v.33 no.6
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    • pp.724-735
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    • 2023
  • NdgR, a global regulator in soil-dwelling and antibiotic-producing Streptomyces, is known to regulate branched-chain amino acid metabolism by binding to the upstream region of synthetic genes. However, its numerous and complex roles are not yet fully understood. To more fully reveal the function of NdgR, phospholipid fatty acid (PLFA) analysis with gas chromatography-mass spectrometry (GC-MS) was used to assess the effects of an ndgR deletion mutant of Streptomyces coelicolor. The deletion of ndgR was found to decrease the levels of isoleucine- and leucine-related fatty acids but increase those of valine-related fatty acids. Furthermore, the defects in leucine and isoleucine metabolism caused by the deletion impaired the growth of Streptomyces at low temperatures. Supplementation of leucine and isoleucine, however, could complement this defect under cold shock condition. NdgR was thus shown to be involved in the control of branched-chain amino acids and consequently affected the membrane fatty acid composition in Streptomyces. While isoleucine and valine could be synthesized by the same enzymes (IlvB/N, IlvC, IlvD, and IlvE), ndgR deletion did not affect them in the same way. This suggests that NdgR is involved in the upper isoleucine and valine pathways, or that its control over them differs in some respect.

Effect of Cultural Conditions on Polysaccharide Production and its Monosaccharide Composition in Phellinus linteus L13202 (배양 조건에 따른 상황 버섯의 다당류 생산 및 단당류 구성 변화)

  • Lee, Jae-Hoon;Cho, Soo-Muk;Ko, Kyung-Soo;Yoo, Ick-Dong
    • The Korean Journal of Mycology
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    • v.23 no.4 s.75
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    • pp.325-331
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    • 1995
  • The effect of cultural conditions on mycelial growth, polysaccharide production in Phellinus linteus and its monosaccharide composition was studied. P. linteus showed the highest growth (0.9 g/100 ml) on glucose but the polysaccharide production was the highest (13.7%) on mannose. The fungus grew very well at neutral pH (0.9 g/100 ml) but the growth was reduced to 0.47 g per 100 ml at alkaline pH. For the different pH, the yield of polysaccharide was in the range of $5{\sim}8%$. The highest yield of 7.94% was obtained at pH 5. Also a variation in monosaccharide composition was observed for different carbon sources and pH. The composition ranges of glucose, mannose, and galactose of polysaccharide were $80{\sim}95%,\;3{\sim}12%,\;and\;2{\sim}10%$ depending on carbon sources, respectively. In contrast, the variation of composition range of three monosaccharides was narrower for different pH than that for carbon sources. These results suggested the possibility of the improvement of production and the physiological modification of the polysaccharide.

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Analysis of Attached Algae and Microbial Community Structure in Sedimentation Basin of Water and Wastewater Treatment Plant (정수 및 하수처리공정 중 침전지 부착조류 및 미생물 군집구조 해석)

  • Lim, Byung-Ran;Ahn, Kyu-Hong;Song, Kyung-Gun;Park, Yu-Jung;Jun, Dae-Young
    • Journal of Korean Society of Water and Wastewater
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    • v.19 no.1
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    • pp.61-67
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    • 2005
  • The objective of this study was to investigate community structure of attached algae and microbes in sedimentation basin of water and wastewater treatment plants by using respiratory quinone profile. There was an evident difference, in microbial community structure and attached algae species, between inclination plate settler and drainage canal in the sedimentation basin. The algae was composed of species in following order; Chlorophyceae>Bascillariophyceae>Cyanophyceae. The dominant quinone types of attached microorganisms in the wastewater treatment plant were plastoquinone (PQ)-9, vitamin(V)K-1 followed by UQ-8, but those for water treatment plant were VK-1, PQ-9 followed by UQ-8. These results suggests that nutrients, seasons and material of sedimentation basin have notable influence on composition of attached algae and microbial community structure in water and wastewater treatment plants.