• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
• /
• 2004.07b
• /
• pp.845-848
• /
• 2004

High throughput analysis using a DNA chip microarray is powerful tool in the post genome era. Less labor-intensive and lower cost-performance is required. Thus, this paper aims to develop the multi-channel type label-free DNA chip and detect SNP (Single nucleotide polymorphisms). At first, we fabricated a high integrated type DNA chip array by lithography technology. Various probe DNAs were immobilized on the microelectrode array. We succeeded to discriminate of DNA hybridization between target DNA and mismatched DNA on microarray after immobilization of a various probe DNA and hybridization of label-free target DNA on the electrodes simultaneously. This method is based on redox of an electrochemical ligand.

• Korean Journal of Veterinary Service
• /
• v.30 no.4
• /
• pp.489-496
• /
• 2007

An oligonucleotide microarray system was developed for the simultaneous detection of porcine epidemic diarrhea virus, transmissible gastroenteritis virus, porcine enteric calicivirus, porcine group A and C rotavirus. RNAs of the reference viruses and porcine diarrhea samples were extracted and amplified using one-step multiplex RT-PCR in the presence of cyanine 5-dCTP and hybridized on the microarray chip that spotted the virus-specific oligonucleotides. This system were approximately 10-to 100-fold higher in sensitivity than conventional RT-PCR, and the assay time was less than 3 hours. The relative sensitivity and specificity were 92% and 72.2%, respectively, based on 102 porcine diarrhea samples using RT-PCR as gold standard. These results suggested that the oligonucleotide microarray system in this study be probably more reliable and reproducible means for detecting porcine enteric viruses and that it could be of substantial use in routine diagnostic laboratories.

• 한국생물공학회:학술대회논문집
• /
• 2005.04a
• /
• pp.47-47
• /
• 2005

There are many different surface technologies currently applied for preparation of protein chips. However, it requires innovative surface chemistry for capture proteins to be immobilized on chip surface keeping their conformation and activity intact and their orientation right, while they bind tightly and densely in a given array spot. Proteogen has developed 'ProteoChip BP' coated with novel proprietary linker molecules $(ProLinker^{TM})$ for efficient and robust immobilizations of capture proteins by improving surface properties of molecular captures. It was demonstrated that $ProLinker^{TM}$ gave the best surface performance in preparation of protein microarray chip base plates among others currently available on the market. In particular, the $ProLinker^{TM}-based$ surface chemistry has demonstrated to provide excellent performance in preparation of 'Antibody Chip' for analysis of biomarkers as well as proteome expression profiles. The linker molecule has also shown to be well applicable for development of biosensors and micro-beads as well as protein microarray and nano-array. ProteoChip BP can be used either for preparation of high-density array by using a microarrayer or for preparation of 'Well-on-a-Chip' with low density array, which is better applicable for quantitative analysis of biomarkers or protein-protein interactions. The biomarker assay can be performed either by direct or sandwich methods of fluorescence immunoassay. Application of ProteoChip BP has been well demonstrated by the extensive studies of 1) tumor-marker assays, 2) new drug screening by using 'Integrin Chip' and 3) protein expression profile analysis. Some of experimental results will be presented.

• Proceedings of the Korean Society of Crop Science Conference
• /
• 2005.10b
• /
• pp.328-329
• /
• 2005

• Proceedings of the KSME Conference
• /
• 2003.04a
• /
• pp.899-904
• /
• 2003

Microarrayer makes DNA chip and microarray that contain hundreds to thousands of immobilized DNA probes on surface of a microscope slide. This paper shows the development results for a printing type of microarrayer. It realizes a typical, low-cost and efficient microarrayer for generating low density microarray. The microarrayer is developed by using a robot of three-axes perpendicular type. It is composed of a computer-controlled three-axes robot and a pen tip assembly. The key component of the arrayer is the print-head containing the tips to immobilize cDNA, genomic DNA or similar biological material on glass surface. The robot is designed to automatically collect probes from two 96-well plates with up to 32 tips at the same time. To prove the performance of the developed microarrayer, the general water types of inks such as black, blue and red. The inks are distributed at proper positions of 96 well plates and the three color inks are immobilized on the slide glass under the operation procedure. As the result of the test, it can be shown that it has sufficient performance for the production of low integrated DNA chip consisted of 96 spots within 1 $cm^2$ area.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
• /
• 2001.07a
• /
• pp.757-760
• /
• 2001

We have used the random fluidic self-assembly (RFSA) technique based on the chip pattern of hydrophobic self-assembly layers to assemble microfabricated particles onto the chip pattern. Immobilization of DNA, fabrication of the particles and the chip pattern, arrangement of the particles on the chip pattern, and recognition of each using DNA fluorescence measurement were carried out. Establishing the walls, the arrangement stability of the particles was improved. Each DNA is able to distinguish by using the lithography process on the particles. Advantages of this method are process simplicity, wide applicability and stability. It is thought that this method can be applicable as a new fabrication technology to develop a minute integration type biosensor microarray.

• BMB Reports
• /
• v.35 no.5
• /
• pp.532-535
• /
• 2002

To establish a method to evaluate the quality of the printed microarray and DNA fragments' immobilization. The target gene fragments that were made with the restriction display PCR (RD-PCR) technique were printed on a superamine modified glass slide, then immobilized with UV cross-linking and heat. This chip was hybridized with universal primers that were labeled with cy3-dUTP, as well as cDNA that was labeled with cy3-dCTP, as the conventional protocol. Most of the target gene fragments on the chip showed positive signals, but the negative control showed no signal, and vice versa. We established a method that enables an effective evaluation of the quality of the microarrays.

• Proceedings of the Korea Information Processing Society Conference
• /
• 2003.11b
• /
• pp.733-736
• /
• 2003

본 논문은 DNA microarray chip 을 사용한 실험 결과로 생산되는 대량의 데이터를 효율적으로 관리하기 위한 LIMS 개발에 대해 기술한다. 기존의 상용 LIMS 는 보편적 패턴과 방식을 정규화하여 제공하기 때문에 실험실의 고유한 방식을 포함하긴 어렵다. 본 논문에서는 유연성 있는 LIMS 를 개발하기 위해 특정 실험 중심으로 설계하면서 MAGE-OM 의 표준을 따르도록 디자인하였고, HYLIMS manager 라는 Local Application 과 검색을 주로 이용하는 사용자를 위하여 Web 검색 시스템을 구현하였다. 데이터베이스의 부하를 줄이기 위해 데이터 저장용 DB 와 검색용 DB 를 구분하였고, 데이터를 타입과 처리 형태에 따라 분류하여 관리하였으며 데이터 보안을 위해 실험 관리자가 사용자의 접근 제한을 설정 할 수 있도록 하였다.

• Proceedings of the Korean Statistical Society Conference
• /
• 2001.11a
• /
• pp.149-153
• /
• 2001

본 논문은 microarray를 분석하기위한 표준화에 대한 여러 방법들을 소개하고 비교해보았다. Microarray 연구는 Human Genome Project에서 파생된 여러 생명공학 기술 중 가장 널리 사용되는 기술로 기존에는 하지 못했던 총체적인 유전자의 발현상황을 탐색할 수 있다는 장점을 지니고 있으나, 자료들에 일정한 패턴이 나타나거나 잡음이 첨가되어 정보의 추출이 용의하지 않다는 단점을 지니고 있다. 특히 자료에 일정한 패턴이 있는 경우에 올바르지 못한 결론을 이끌어낼 수도 있기에 이 패턴을 제거하는 표준화작업은 microarray 분석에 있어서 매우 중요한 처리과정이다. 본 논문에서는 표준화방법들을 소개하고 각각 가지고 있는 장단점을 실제 국내에서 얻어진 자료를 통해 비교하였고, 그 결과 LOWESS 적합을 통한 표준화방법이 타 방법에 비해 유용한 점이 많음을 확인할 수 있었다.

• Proceedings of the KIEE Conference
• /
• 2002.11a
• /
• pp.263-265
• /
• 2002

We have used the secondary-step immobilization methods based on the chip pattern of hydrophobic self-assembly layers to assemble microfabricated particles onto the chip pattern. Immobilization of DNA, fabrication of the particles and the chip pattern, arrangement of the particles on the chip pattern, and recognition of each using DNA fluorescence measurement were carried out. Establishing the walls, the arrangement stability of the particles was improved. Each DNA is able to distinguish by using the lithography process on the particles. Advantages of this method are process simplicity, wide applicability and stability. It is thought that this method can be applicable as a new fabrication technology to develop a minute integration type biosensor microarray.