• 한국세라믹학회지
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• 제53권1호
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• pp.50-55
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• 2016

In order to make a highly ordered three-dimensionally macro-porous structure of zirconia ceramics, porogen precursors PMMA beads were prepared by emulsion polymerization using acrylic monomer. The monodisperse PMMA latex beads were closely packed by centrifugation as a porogen template for the infiltration of zirconium acetate solution. The mixed compound of PMMA and zirconium acetate was dried. According to the firing schedule, dry compacts of PMMA and zirconium acetate were calcined at $475^{\circ}C$ to obtain micro-, macro-, and meso- structures of polycrystalline zirconia with monodispersed porosity. Inorganic frameworks composed of $ZrO_2$ were formed and showed a three Dimensionally Ordered Microstructure [3DOM] of $ZrO_2$ ceramics. The obtained $ZrO_2$ skeleton was calcined at $710^{\circ}C$. The 3DOM $ZrO_2$ skeleton showed color tuning in solutions such as deionized [DI] $H_2O$ and/or methanol. The monodispersed crystalline micro-structure with micro/meso porosity was observed by FE-SEM.

• 대한기계학회논문집B
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• 제31권4호
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• pp.392-397
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• 2007

We developed a microfluidic platform able to control the trap and release of magnetic beads used for separation of a specific biomolecules. The magnetic beads can be trapped and released conditionally by controlling the difference between the Stokes force induced by the fluid flow and magnetic force resulting from a permanent magnet. The permanent magnet of CoNiP alloy is electroplated. It is characterized to have the 1369 Oe of coercivity, 1762 Gauss of remanence, and 0.603MGOe of (BH)max. Through the experimental and numerical investigation, the magnetic beads are trapped under the flow velocity of 17 ${\mu}m/s$ and are released perfectly above the velocity of 174 ${\mu}m/s$.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2008년도 추계학술대회A
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• pp.1509-1514
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• 2008

To fabricate microsized poly(methyl methacrylate)(PMMA) beads of uniform size and density on poly(ethylene terephthalate) (PET) fis, we introduce an electro spraying technique that uses a target electrode applied with an ac electric fid. Using the apparatus and various material properties, we could obtain uniform size PMMA beads which were deposited on the thin PET film. The optical properties, transmittance and light diffusivity of the fis electro sprayed with the PMMA were characterized. The results show that the sprayed beads appear to act as a good optical diffuser, like microlenses. To understand the effect of process variables, applied field conditions and rheological properties, the morphological pictures of the deposited particles were investigated through the optical and scanning electron microscope.

• 한국재료학회지
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• 제24권10호
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• pp.532-537
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• 2014

In this study, highly sensitive hydrogen micro gas sensors of the multi-layer and micro-heater type were designed and fabricated using the micro electro mechanical system (MEMS) process and palladium catalytic metal. The dimensions of the fabricated hydrogen gas sensor were about $5mm{\times}4mm$ and the sensing layer of palladium metal was deposited in the middle of the device. The sensing palladium films were modified to be nano-honeycomb and nano-hemisphere structures using an anodic aluminum oxide (AAO) template and nano-sized polystyrene beads, respectively. The sensitivities (Rs), which are the ratio of the relative resistance were significantly improved and reached levels of 0.783% and 1.045 % with 2,000 ppm H2 at $70^{\circ}C$ for nano-honeycomb and nano-hemisphere structured Pd films, respectively, on the other hand, the sensitivity was 0.638% for the plain Pd thin film. The improvement of sensitivities for the nano-honeycomb and nano-hemisphere structured Pd films with respect to the plain Pd-thin film was thought to be due to the nanoporous surface topographies of AAO and nano-sized polystyrene beads.

• Environmental Engineering Research
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• 제15권3호
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• pp.149-156
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• 2010

The objective of this study was to investigate microbial removal using layered double hydroxides (LDHs) and iron (hydr)oxides (IHs) immobilized onto granular media. Column experiments were performed using calcium alginate beads (CA beads), LDHs entrapped in CA beads (LDH beads), quartz sand (QS), iron hydroxide-coated sand (IHCS) and hematite-coated sand (HCS). Microbial breakthrough curves were obtained by monitoring the effluent, with the percentage of microbial removal and collector efficiency then quantified from these curves. The results showed that the LDH beads were ineffective for the removal of the negatively-charged microbes (27.7% at 1 mM solution), even though the positively-charged LDHs were contained on the beads. The above could be related to the immobilization method, where LDH powders were immobilized inside CA beads with nano-sized pores (about 10 nm); therefore, micro-sized microbes (E. coli = 1.21 ${\mu}m$) could not diffuse through the pores to come into contact with the LDHs in the beads, but adhere only to the exterior surface of the beads via polymeric interaction. IHCS was the most effective in the microbial removal (86.0% at 1 mM solution), which could be attributed to the iron hydroxide coated onto the exterior surface of QS had a positive surface charge and, therefore, effectively attracted the negatively-charged microbes via electrostatic interactions. Meanwhile, HCS was far less effective (35.6% at 1 mM solution) than IHCS because the hematite coated onto the external surface of QS is a crystallized iron oxide with a negative surface charge. This study has helped to improve our knowledge on the potential application of functional granular media for microbial removal.

• Journal of Magnetics
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• 제16권4호
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• pp.363-367
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• 2011

We have designed, fabricated and demonstrated a novel micro-system for programmable magnetic actuation using magnetic elliptical pathways on Si substrates. Lithographically patterned soft NiFe ellipses are arranged sequentially perpendicular to each other as stepping stones for the transport of magnetic beads. We have measured the magnetization curve of the ellipsoid ($9\;{\mu}m{\times}4\;{\mu}m{\times}0.1\;{\mu}m$) elements with respect to the long and short axes of the ellipse. We found that the magnetization in the long axis direction is larger than that in the short axis direction for an applied field of ${\leq}$ 1,000 Oe, causing a force on carriers that causes them to move from one element to another. We have successfully demonstrated a micro-system for the magnetic actuation of biomolecule carriers of superparamagnetic beads (Dynabead$^{(R)}$ 2.8 ${\mu}m$) by rotating the external magnetic field. This novel concept of magnetic actuation is useful for future integrated lab-on-a-chip systems for biomolecule manipulation, separation and analysis.

• Journal of Microbiology and Biotechnology
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• 제31권9호
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• pp.1323-1329
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• 2021

Micro-scale magnetic beads are widely used for isolation of proteins, DNA, and cells, leading to the development of in vitro diagnostics. Efficient isolation of target biomolecules is one of the keys to developing a simple and rapid point-of-care diagnostic. A zinc finger protein (ZFP) is a double-stranded (ds) DNA-binding domain, providing a useful scaffold for direct reading of the sequence information. Here, we utilized two engineered ZFPs (Stx2-268 and SEB-435) to detect the Shiga toxin (stx2) gene and the staphylococcal enterotoxin B (seb) gene present in foodborne pathogens, Escherichia coli O157 and Staphylococcus aureus, respectively. Engineered ZFPs are immobilized on a paramagnetic bead as a detection platform to efficiently isolate the target dsDNA-ZFP bound complex. The small paramagnetic beads provide a high surface area to volume ratio, allowing more ZFPs to be immobilized on the beads, which leads to increased target DNA detection. The fluorescence signal was measured upon ZFP binding to fluorophore-labeled target dsDNA. In this study, our system provided a detection limit of ≤ 60 fmol and demonstrated high specificity with multiplexing capability, suggesting a potential for development into a simple and reliable diagnostic for detecting multiple pathogens without target amplification.

• 보존과학회지
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• 제20권
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• pp.105-117
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• 2007

사적 제450호 사천 늑도 유적에서 출토된 구슬의 분석을 통하여 이 유적의 고대유리 문화의 특성을 확인하고 이를 경상도 지역 출토 고대유리의 특성과 비교하였다. 시료는 광학현미경과 SEM을 이용하여 미세구조를 관찰하고 비중측정을 통해 물성을 확인하였다. SEM-EDS를 이용하여 유리의 구성 요소인 융제, 안정제, 착색제의 조성을 분석하였으며, XRD를 이용하여 기타 구슬시료의 재질을 조사하였다. 조개구슬로 분류한 백색 불투명 구슬은 비정질의 규산염계 물질로 확인되었으며, 청색 계열의 유리구슬은 포타쉬유리군($SiO_2-K_2O$ group). LCA(Low-CaO, $Al_2O_3$) 계통으로 색상별로 벽색과 청록색은 LCA-A(LCA-CaO<$Al_2O_3$) 계통, 감색은 LCA-B(LCA-CaO>$Al_2O_3$) 계통으로 확인된다. 이를 경상도지역에서 출토된 고대유리와 비교하면 AD $2{\sim}3$세기 이전의 포타쉬유리와 유사한 특징을 보인다.

• 한국자기학회지
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• 제15권2호
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• pp.76-80
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• 2005

환경정화 등의 기능성을 부여할 수 있는 고분자자성체가 토양 오염물처리에 사용될 수 있는지 여부를 살펴보았다. 본 연구에서는 수산화기를 지닌 마이크로 고분자자성체를 분체로 걸러진 토양$(<0.025{\cal}mm)$, 물과 함께 혼합하고 교반시킨 후 1.2 Tesla의 자력을 지닌 자석으로 자성체를 분리하였다. 이때 고려되어진 인자는 토양과 고분자자성체의 비율, 토양과 물의 비율, 반응용기의 크기 그리고 자력의 크기였다. 토양과 고분자자성체의 분리실험 결과, 고분자자성체와 물의 양이 분리도에 영향을 미친 반면 반응용기의 크기와 자력은 이 소규모의 실험에서 별다른 영향이 없었다. 본 실험을 통하여 전체적으로 반응조건을 최적화시켜 $90{\~}100{\%}$의 분리도를 달성할 수 있었다. 이로서 기능성고분자를 환경 처리에 적용함에 있어서, 수처리 뿐만 아니라 토양처리에도 사용이 가능함을 확인하였다.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2003년도 생물공학의 동향(XIII)
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• pp.717-720
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• 2003

A chromogenic biosensor employing microfluidics on a chip has been developed for the determination of high-density lipoprotein (HDL) cholesterol (HDL-C) in human serum. We have investigated a plain and effective method to immobilize enzymes within the microchip without chemically modifying micro-channel or technically micro-fabricating column reactor and fluid channel network. In assessing risk factors of coronary heart disease, a micro-chip system would minimize requirements of instrument and reagent handling.