• Current Research on Agriculture and Life Sciences
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• 제4권
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• pp.119-123
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• 1986

본(本) 연구(硏究)는 마우스의 이유시체중(離乳時體重) 및 60일령(日齡) 체중(體重)에 대한 유전자(遺傳子) 효과(效果)를 구명하기 위하여, BALB/c, CBA, C3H 및 C57BL의 4 계통(系統)을 양면교잡(兩面交雜)시켜 생산(生産)된 후대(後代) 343마리를 대상으로 조사(調査) 분석(分析)한 것이다. 공시(供試)된 마우스는 1984년(年) 11월(月)부터 1985년(年) 2월(月)까지 경북대학교(慶北大學校) 농과대학(農科大學) 부속동물사육장(附屬動物飼育場)에서 사육(飼育)되었으며, Harvey의 방법(方法)에 의하여 일반결합능력(一般結合能力), 모체효과(母體效果), 특수결합능력(特殊結合能力) 및 상반교잡(相反交雜) 효과(效果) 등(等)이 분석(分析)되었다. 일반결합능력(一般結合能力) 효과(效果)는 이유시체중(離乳時體重)에서 -0.6033~0.5298 그리고 60일령(日齡) 체중(體重)에서 -0.5086~1.0012로 추정(推定)되었으며, 두 형질(形質)에 대한 일반결합능력(一般結合能力) 효과(效果)는 BALB/c와 C57BL이 CBA와 C3H보다 우수하였다(P<0.05). 모체효과(母體效果)는 C3H가 BALB/c보다 크게 나타났다(P<0.05). 모체효과(母體效果)는 이유시체중(離乳時體重)에서 -0.9678~0.4609 그리고 60일령(日齡) 체중(體重)에서 -1.1886~0.7029로 추정(推定)되었다. 특수결합능력(特殊結合能力) 효과(效果)도 유의성(有意性)이 인정(認定)되었는데(P<0.05), 그 효과(效果) 추정치(推定値)는 이유시체중(離乳時體重)에서 -0.1999~0.3380 그리고 60일령(日齡) 체중(體重)에서 -0.4056~0.3317 이었다. 상반교잡(相反交雜) 효과(效果)는 BALB/c${\times}$C57BL 및 BALB/c${\times}$C3H에서 크게 나타났는데 이유시체중(離乳時體重)에 있어서 효과(效果) 추정치(推定値)는 각각(各各) -0.5049 및 0.4972 그리고 60일령(日齡) 체중(體重)에 있어서 효과(效果) 추정치(推定値)는 각각(各各) -1.0336 및 1.2793이었다.

• Asian Pacific Journal of Cancer Prevention
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• 제14권11호
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• pp.6669-6672
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• 2013

Colon cancer (CRC) is a serious health problem throughout the world. Development of novel drugs without side effects for this cancer is crucial. Luteolin (LUT), a bioflavonoid, has many beneficial effects such as antioxidant, anti-inflammatory and anti-proliferative potential. was a potent chemical carcinogen used for the induction of colon cancer. Colon carcinogenesis was initiated by intraperitoneal injection of azoxymethane (AOM) to mice at the dose of 15 mg/body kg weight in Balb/C mice for 3 weeks. Mice were treated with LUT at the dose of 1.2 mg/body kg weight orally. Mitochondrial enzymes such as isocitrate dehydrogenase (ICDH), ${\alpha}$-keto dehydrogenase (${\alpha}$-KDH), succinate dehydrogenase (SDH) and the activities of respiratory chain enzymes NADH dehydrogenase and cytochrome c oxidase were found to be elevated in AOM-treated animals. Treatment with LUT decreased the activities of all the parameters significantly. Hence, LUT might be a potent anticancer agent against colorectal cancer.

• 대한수의학회지
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• 제61권2호
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• pp.12.1-12.9
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• 2021

The mosquito-borne pathogen Zika virus may result in neurological disorders such as Guillain-Barré syndrome and microcephaly. The virus is classified as a member of the Flaviviridae family and its wide spread in multiple continents is a significant threat to public health. So, there is a need to develop animal models to examine the pathogenesis of the disease and to develop vaccines. To examine the clinical profile during Zika virus infection, we infected neonatal and adult wild-type mice (C57BL/6 and Balb/c) and compared the clinical signs of African-lineage strain (MR766) and Asian-lineage strain (PRVABC59, MEX2-81) of Zika virus. Consistent with previous reports, eight-week-old female Balb/c mice infected with these viral strains showed no changes in body weight, survival rate, and neurologic signs, but demonstrated increases in the weights of spleens and hearts. However, one-day-old neonates showed significantly lower survival rate and body weight with the African-lineage strain than the Asian-lineage strain. These results confirmed the pathogenic differences between Zika virus strains. We also evaluated the clinical responses in neonatal and adult mice of different strains. Our findings suggest that these are useful mouse models for characterization of Zika virus for vaccine development.

• 생명과학회지
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• 제19권10호
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• pp.1444-1450
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• 2009

본 연구를 통해 BALB/c 마우스에서 quercetin의 경구투여가 PCL로 유도된 접촉성 피부 알레르기의 예방에 미치는 효과에 대해 알아보았다. Quercetin을 투여 농도에 따라 대조군(0 mg/kg), 저용량군(50 mg/kg), 고용량군(100 mg/kg)으로 나누고 8일에 걸쳐 총 8번 경구투여를 실시한 후 알레르기 유발물질인 PCL을 마우스의 양쪽 귀에 감작시켜 접촉성 피부 알레르기를 유발 시켰다. Quercetin의 투여 농도별 ear swelling 변화를 확인한 결과 quercetin을 투여하지 않은 대조군에 비해 quercetin을 투여한 군의 ear swelling 증가폭이 낮게 나타났고, 100 mg/kg (고용량군)에서 농도 유의적으로 ear swelling의 증가폭이 현저히 낮게 나타났다. Quercetin의 투여 농도에 따른 혈청 내 염증성 매개 물질의 농도 변화를 알아보기 위한 IgE 및 histamine level 측정 결과에서 quercetin을 투여하지 않은 대조군에 비해 quercetin을 투여한 50 mg/kg (저용량군), 100 mg/kg (고용량군)에서 낮은 수준의 IgE, histamine 수치가 나왔다. H&E염색과 Toluidine blue stain을 통한 조직병리학적 검사결과에서 quercetin을 투여한 고용량군의 귀 두께가 대조군에 비해 얇게 관찰되었고, 비만세포의 유무를 알아보기 위한 Toluidine blue stain의 결과 대조군에 비해 고용량군에서 적은 수의 비만세포들이 관찰되었다. 따라서 ear swelling과 IgE, histamine level, 조직병리학적 결과를 종합해 봤을 때 식물성 flavonoid 성분인 quercetin은 접촉성 피부 알레르기의 예방에 상당한 효과가 있다고 판단되며, 부작용이 발생하는 기존의 치료제를 대체할 수 있는 후보 물질로써 중요한 가치가 있다고 사료된다.

• 대한수의학회지
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• 제41권4호
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• pp.529-533
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• 2001

칠면조에서 분리된 avian pneumovirus(APV)의 포유동물 전파 여부를 확인하기 위해 Balb/c 쥐에 감염을 시도하였다. APV롤 3주, 5주, 7주령의 실험동물에 비강내 감염시킨 후 감염동물과 동거동물의 임상증상 및 조직내 바이러스 유전자 존재 여부등을 확인하였다. 감염 동물 및 동거 동물에서 임상증상은 나타나지 않았으나 감염 6일후에 혈액, 폐, 기도, 구강 및 직장 시료에서 RT-PCR 방법으로 APV의 유전자를 검출할 수 있었다. 14일 후에는 혈액에서는 유전자가 검출되지 않았으나 혈청내 항체를 확인할 수 있었다. 동거동물에서는 폐와 직장시료에서 PCR로 APV를 검출할 수 있었다. 이와 같은 결과로 칠면조 유래 APV는 실험쥐에 감염되어 직접 접촉에 의한 전파도 가능하다는 것을 알수 있었다.

• 생약학회지
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• 제31권2호
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• pp.142-148
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• 2000

The purpose of this research was to investigate effects of Samultang water extract (SMT) on cytokine production from immune cells during the late stage of pregnancy in BALB/c mice. SMT(500 mg/kg) was administered p.o. once a day for 7 days, and then thymocytes and peritoneal macrophages were separated. At the late stage of pregnant mice, the proliferation of thymocytes and the production of ${\gamma}-interferon$ in thymocytes were decreased as compared with normal group, but the production of interleukin-2 and interleukin-4 was increased. The production of tumor necrosis $factor-{\alpha}$, nitric oxide and phagocytic activity in peritoneal macrophage was increased as compared with normal group. At the late stage of pregnant mice administered with SMT, the production of interleukin-2 in thymocytes was decreased as compared with a pregnant group, but the proliferation of thymocytes, the production of ${\gamma}-interferon$ and interleukin-4 was increased. The production of tumor necrosis $factor-{\alpha}$ and nitric oxide in peritoneal macrophages were decreased as compared with a pregnant group, but phagocytic activity were increased. These results suggest that SMT has the regulative action on immune function of thymocytes and peritoneal macrophages at the late stage of pregnant mice.

• 대한한의학방제학회지
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• 제7권1호
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• pp.107-120
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• 1999

The purpose of this Study was to investigate effects of KaegiBokryengHwan(KBH) on anti-tumor, immunocytes and nitric oxide(NO). This Study estimated the proliferation of L1210 cell lines, HeLa cell lines, SK-OV3 cell lines, MCF-7 cell lines, balb/c mouse 3T3 cell lines, mouse thymocytes and mouse splenocytes and NO production from peritoneal macrophages in vitro. and estimated the proliferation of L1210 cells, mouse thymocytes and splenocytes and NO production from peritoneal macrophages and body weight in L1210 cells-transplanted mice in vivo. The result were obtained as follow ; 1. KBH inhibited significantly SK-OV3 cell lines in vitro. 2. KBH was accelerate significantly the proliferation of balb/c mouse thymocytes in vitro. 3. KBH increased significantly NO production from peritoneal macrophages in vitro. 4. KBH didn't effect the cytotoxicity of L1210 cells in L1210 cells-transplanted mice. 5. KBH was accelerate the proliferation of splenocytes in L1210 cells-transplanted mice. 6. KBH increased NO production from peritoneal macrophages in L1210 cells-transplanted mice. 7. KBH increased the body weight as comparing with control group in L1210 cells-transplanted mice.

• Preventive Nutrition and Food Science
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• 제4권4호
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• pp.260-264
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• 1999

Korean traditional fermented soy paste (doenjang) prolonged the life span of Balb/c mice injected with the sarcoma-180 cells. The activities of liver enzymes, such as xanthine oxidase, aminopyrine N-demethylase, aniline hydroxylase, ${\gamma}$-glutamylcysteine synthetase, glutathione reductase and glutathione S-transferase (GST), and the contents of lipid peroxide and glutathione were determined from the sarcoma-180 cell injected mice that were treated with methanol extracts from doenjang, miso and soybean. The content of lipid peroxide and the activity of xanthine oxidase in the liver of Balb/c mice which were increased by the transplantation of the sarcoma-180 cells were decreased by treatment with the methanol extract from doenjang. But the activities of aminopyrine N-dementhylase and aniline hydroxylase were not affected by the treatment of methanol extracts from doenjang to the mice injected with the sarcoma-180 cells. The content of glutathione, the activities of glutamylcysteine synthetase, glutathione reductase and glutathione S-transferase decreased by the injection of the sarcoma-180 were recovered considerably by the treatment of the methanol extract from doenjang.

• Food Science and Biotechnology
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• 제27권6호
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• pp.1791-1799
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• 2018

In this study, the immunomodulatory activity of oligopeptide (CP) derived from solid-state fermented cottonseed meal were investigated in immunosuppressed BALB/c mice models by treatment with cyclophosphamide (CY). Results indicated that oligopeptide increased the thymus and spleen indices of CY-treated mice. The count of plague forming cells (PFC) and the content of half serum hemolysis ($HC_{50}$) in immunosuppressive mice were restored to the normal level in CP-10 and CP-20 groups while the cytokines interleukin (IL)-2, IL-6, and tumor necrosis factor alpha (TNF-${\alpha}$) were increased significantly in CP-20 group. Similar increasing the immunoglobulin of IgG and IgM content in the serum of CP-10 group were also observed. These findings indicated that oligopeptide derived from solid-state fermented cottonseed meal had a strong immune-enhancing activity as well as a protective effect against immunosuppression induced by cyclophosphamide in mice.

• International Journal of Stem Cells
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• 제15권3호
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• pp.291-300
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• 2022

Background and Objectives: Many preclinical studies have been conducted using animal disease models to determine the effectiveness of human mesenchymal stem cells (hMSCs) for treating immune and inflammatory diseases based on the belief that hMSCs are not immunogenic across species. However, several researchers have suggested xenogeneic immune responses to hMSCs in animals, still without detailed features. This study aimed to investigate a xenogeneic humoral immune response to hMSCs in mice in detail. Methods and Results: Balb/c mice were intraperitoneally injected with adipose tissue-derived or Wharton's jelly-derived hMSCs. Sera from these mice were titrated for each isotype. To confirm specificity of the antibodies, hMSCs were stained with the sera and subjected to a flow cytometic analysis. Spleens were immunostained for proliferating cell nuclear antigen to verify the germinal center formation. Additionally, splenocytes were subjected to a flow cytometric analysis for surface markers including GL-7, B220, CD4, CD8, CD44, and CD62L. Similar experiments were repeated in C57BL/6 mice. The results showed increased IgG₁ and IgG_2a titers in the sera from Balb/c mice injected with hMSCs, and the titers were much higher in the secondary sera than in the primary sera. These antibodies were specifically stained the hMSCs. Germinal centers were observed in the spleen, and flow cytometric analysis of the splenocytes showed higher frequencies of centroblasts (B220⁺ GL7⁺) and memory T cells (CD62L⁺ CD44⁺) both in CD4⁺ and CD8⁺ subsets. Similar results were obtained for C57BL/6 mice. Conclusions: hMSCs induced a humoral immune response in mice, with characters of T cell-dependent immunity.