• 해양환경안전학회:학술대회논문집
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• 해양환경안전학회 2005년도 추계학술대회지
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• pp.125-132
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• 2005

해양은 인류가 앞으로 풀어야 할 숙명적인 문제인 식량문제, 자원문제, 환경문제를 해결할 수 있는 마지막 보루이다 이러한 점을 감안하여 국내 산$\cdot$학$\cdot$연 전문가가 참여하여 추진한 「해양과학기술(MT) 개발 계획」이 2004년 7월 28일 대통령을 위원장으로 하는 국가과학기술위원회 심의에 통과됨으로써 우리나라에서도 본격적으로 MT개발을 추진할 기반을 마련하게 되었다. 또한, 2005년 6월부터 「MT개발계획」을 효율적으로 추진하기 위한 후속조치로 해양과학기술 로드맵(MTRM)을 추진중에 있다. 본 논문에서는 MT의 국내외 기술개발동향과 현재 직면한 현안문제 및 MTRM 추진현황을 점검하고, MT R&D 전문기관 설치$\cdot$운영 및 예산확보 등 MT 활성화 방안을 제시하였다.

• 한국결정학회지
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• 제2권2호
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• pp.28-31
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• 1991

4-크로로-4'-메톡시-2-니트로디페닐아민, (C12H11N2O3CL, FW=278.70)의 단것세포 상수는 a=8,169(3), b=8.883(1), c=9.150(1) h, α =82. 98(1), β=104.80(2), y=101.43(2)", V=627.3 A3, F(000)=288.0, Dc=1.48g/cm3, u=3.06cm-1, 7=295" K, 공간군 Pi, 번호 2, 삼사 정계 이며, Z=2이다. λ (Mo-Ka)=0.7107A을 사용 하여 수집한 독립적인 회절 반점 1541개로 구조분 석한 최종 신뢰도 값은 각각 R=0.032, Rw=0.033 이며, S=0.46이다. 본 화합물은 암모니아의 두개 의 수소 대신에 4-크로로-페닐기와 4-메톡시-페닐기로 치환된 물질로써, 질소와 두 페닐기 사이의 각과 결합거리 들은 각각 125.42", 1.362 및 1.428 A 인바 수소와 함께 SP2_혼성결합을 하고있다. 질소를 포함한 두 면간 각은 63.29"이다. 분자간 어 떠 한 수소결합을 하고 지지 않다.

• 생약학회지
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• 제48권2호
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• pp.125-133
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• 2017

In this study, we investigated the total phenolic and flavonoid contents, component analysis, antioxidative activity and cellular protective effects against oxidative stress on human skin cells in 50% ethanol extract, ethyl acetate fraction and aglycone fraction obtained from Gynostemma pentaphyllum (G. pentaphyllum) Makino. The DPPH (1,1-diphenyl-2-picrylhydrazyl) scavenging activites ($FSC_{50}$) of the 50% ethanol extracts, ethyl acetate fraction and aglycone fraction were 246.8, 147.2, $128.9{\mu}g/mL$, respectively. Reactive oxygen species (ROS) scavenging activities ($OSC_{50}$) of the 50% ethanol extract, ethyl acetate fraction and aglycone fraction on ROS generated in $Fe^{3+}-EDTA/H_2O_2$ system using the luminol-dependent chemiluminescence assay were 37.15, 10.74, $7.19{\mu}g/mL$, respectively. We investigated the cellular protective activity and the results showed that treatment of aglycone fraction ($0.05-0.39{\mu}g/mL$) protect human skin cells in a concentration-dependent manner when the skin cell damages were induced by treating them with $H_2O_2$. These results suggest that extract/fractions of G. pentaphyllum Makino may be applicable as natural antioxidants in cosmetics.

• Applied Biological Chemistry
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• 제49권2호
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• pp.125-128
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• 2006

Schizonepeta tenuifolia Briq. are used in folk medicine as common cold, sore throat, headache and skin infection in Korea, China and Japan. To compare the contents of domestic and Chinese Schizonepeta tenuifolia quantitative HPLC analysis was performed using a Luna $C_{18}$ column $(4.6{\times}250\;mm,\;5{\mu}m)$, with 1 ml/min of flow rate of 0.01% formic acid in water : acetonitrile = 50 : 50 under UV 254 nm of detector. Pulegone was detected at retention time of about 17.14 min.

• 한국수산과학회지
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• 제33권2호
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• pp.103-109
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• 2000

열목어를 비롯한 산천어, 시마연어, 연어, 무지개송어 등 우리나라 연어아과 어류의 집단구조분석을 위한 기초자료를 얻기 위하여, 미토콘드리아 ribosomal RMA 유전자 영역의 염기서열변이를 비교${\cdot}$분석하였다. 미토콘드리아 DNA의 125 rRNA(945 bases, 열목어 의 경우 946 bases), Valine transfer RNA (72 bases), 및 16S rRNA(1513 bases) 등 3개의 유전자 영역에 걸쳐, 최대 2531 bases의 염기서열을 PCR/direct sequencing하여 얻었는데, 모든 염기변이중 전이가 월등히 우세하게 나타났으며, 종내${\cdot}$종간변이율은 모두 $0.5{\%}$이하로 낮게 나타나, 다른 영역에 비해 rRNA 유전자 영역에서의 염기서열이 매우 보존적임을 보여주었다. 또한, 미토콘드리아 rRNA 유전자 염기서열은 연어류의 속 (genus)단계 이상에서 집단분류표지인자로 유용하게 쓰일 수 있으리라 사료되어진다. 미토콘드리아 rRNA 염기서열자료를 기초로 구성된 phylogenetic tree를 통해 이들 종간의 진화적인 유연관계를 살펴본 결과, 시마연어가 무지개송어보다는 연어와 더 근연인 것으로 나타났으며, 열목어는 가장 유연이 먼 종임을 확인할 수 있었다.

• Genomics & Informatics
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• 제17권4호
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• pp.48.1-48.6
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• 2019

Over the last decade, genome-wide association studies (GWASs) have provided an unprecedented amount of genetic variations that are associated with various phenotypes. However, previous GWAS were mostly conducted in European populations, and these biased results for non-Europeans may result in a significant reduction in risk prediction for non-Europeans. An issue with the early GWAS was the winner's curse problem, which led to misleading results when constructing the polygenic risk scores (PRS). Therefore, more non-European population-based studies are needed to validate reported variants and improve genetic risk assessment across diverse populations. In this study, we validated 422 variants independently associated with glycemic indexes, liver enzymes, and type 2 diabetes in 125,872 samples from a Korean population, and further validated the results by assessing publicly available summary statistics from European GWAS (n = 898,130). Among the 422 independently associated variants, 284, 320, and 361 variants were replicated in Koreans, Europeans, and either one of the two populations. In addition, the effect sizes for Koreans and Europeans were moderately correlated (r = 0.33-0.68). However, 61 variants were not replicated in both Koreans and Europeans. Our findings provide valuable information on effect sizes and statistical significance, which is essential to improve the assessment of disease risk using PRS analysis.

• 가정∙방문간호학회지
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• 제22권2호
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• pp.246-255
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• 2015

Purpose: The study was conducted to develop the Nursing Practice Capacity scale for evaluating achievement of education objectives among students in nursing school. Methods: This was a methodological study. The scale development process included generation of initial items, verification of content validity, selections of secondary items, preliminary study, and extraction of final items. The participants were five graduates 1 to 4 years after graduation (for the preliminary study) and 125 graduates 1 to 4 years after graduation from the nursing school at A University, South Korea. Item analysis, criterion validity, and internal consistency were used to analyze the data. Results: Twenty-six items were selected for the final scale. The scores on the scale were significantly correlated with the Nursing Competency Scale(r=.62, p<.001) and Nursing Performance Scale(r=.63, p<.001). Chronbach's alpha coefficient for the 26 items was .87. Conclusion: The Nursing Practice Capacity scale for evaluating achievement of nursing education objectives has good validity and reliability. The measurement scale can be useful for evaluating the nursing practice capacity of nurses as well as measuring the extent to which graduates have achieved the established education objectives in the education fields.

• 한국보건간호학회지
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• 제24권1호
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• pp.115-125
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• 2010

Purpose: This study was conducted to investigate the knowledge of infectious diseases and the management of children with infectious diseases among daycare facility teachers. Methods: This study was based on survey questionnaires completed by 122 day care facility teachers from 36 daycare center sin one district in Seoul. Results: Seventy three (72.8%) of the participating daycare facility teachers had not received infectious disease prevention education. They recognized that they did not have enough knowledge of infectious diseases. The average knowledge level of infectious diseases was scored as 9.30 on a 0-30 scale, with 0 representing no knowledge and 30 expert knowledge, and compliance level of management of children with infectious diseases was scored as 4.29 on a 0-16 scale, with 0 present no compliance and 16 representing total compliance Confidence of management of children with infectious diseases (r=0.24, p=.031) and the compliance level of management (r=0.35, p=.001) were higher with increased knowledge of infectious diseases. Conclusions: Daycare facility teacher scan lack sufficient knowledge about b infectious diseases. The education about infectious diseases and management of children with infectious diseases is indispensable to prevent trans mission of infectious diseases in daycare facilities.

• 한국식품위생안전성학회지
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• 제34권4호
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• pp.334-339
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• 2019

본 연구는 조제유류 중 "식품의 기준 및 성분규격"에 기준 규격이 설정되어 있지 않으나 제품에 함유되어 있는 베타카로틴에 대해 분석법을 마련하고자 수행하였다. 조제유류에 함유된 베타카로틴 함량 분석을 위해 HPLC를 이용한 분석법을 확립하고 시중에 유통 중인 제품을 대상으로 적용성을 검토하였다. 베타카로틴 표준품을 이용하여 HPLC를 이용한 기기분석조건을 확립하고 시료 중의 베타카로틴을 추출하여 분석하였다. 분석법 검증은 특이성, 직선성, 검출한계 및 정량한계, 정확성, 정밀성에 대해 수행되었다. 0.125~2 mg/L의 농도범위에서 $R^2=0.999$ 이상의 우수한 직선성을 확인할 수 있었으며, LOD와 LOQ는 각각 0.1, 0.2 mg/L였다. 표준물질 첨가법을 이용하여 정확성을 검토하였으며, 81~120%의 회수율을 확인할 수 있었다. 정밀성을 검토한 결과 시료 채취량에 따른 반복성은 RSD값이 2.1~4.9%, 실험실간 교차검증을 통한 실험일자에 따른 재현성은 4.0 RSD(%)로 확인되었다. 본 연구에서 확립된 분석법을 적용하여 조제유류 13건, 성장기용조제식 7건 등 국내 유통 중인 제품 20건에 대해 적용성 검토를 실시한 결과 전체 시료에서 분석이 용이하였으며, 모두 표시기준에 적합함을 확인하였다.

• Genomics & Informatics
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• 제19권4호
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• pp.37.1-37.7
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• 2021

Genome-wide association studies (GWASs) facilitated the discovery of countless disease-associated variants. However, GWASs have mostly been conducted in European ancestry samples. Recent studies have reported that these European-based association results may reduce disease prediction accuracy when applied in non-Europeans. Therefore, previously reported variants should be validated in non-European populations to establish reliable scientific evidence for precision medicine. In this study, we validated known associations with type 2 diabetes (T2D) and related metabolic traits in 125,850 samples from a Korean population genotyped by the Korea Biobank Array (KBA). At the end of December 2020, there were 8,823 variants associated with glycemic traits, lipids, liver enzymes, and T2D in the GWAS catalog. Considering the availability of imputed datasets in the KBA genome data, publicly available East Asian T2D summary statistics, and the linkage disequilibrium among the variants (r² < 0.2), 2,900 independent variants were selected for further analysis. Among these, 1,837 variants (63.3%) were statistically significant (p ≤ 0.05). Most of the non-replicated variants (n = 1,063) showed insufficient statistical power and decreased minor allele frequencies compared with the replicated variants. Moreover, most of known variants showed <10% genetic heritability. These results could provide valuable scientific evidence for future study designs, the current power of GWASs, and future applications in precision medicine in the Korean population.