• Journal of Microbiology and Biotechnology
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• 제31권11호
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• pp.1508-1518
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• 2021

Hsa_circ_0001947 is associated with multiple cancers, but its function in non-small cell lung cancer (NSCLC) is ambiguous and needs further research. The targeting relationship among circ_0001947, miR-661, and downstream of tyrosine kinase 7 (DOK7) was predicted by database and further verified by dual-luciferase reporter assay, while their expressions in cancer tissues and cells were detected by quantitative real-time polymerase chain reaction (qRT-PCR). After transfection, cell biological behaviors and expressions of miRNAs, miR-661 and DOK7 were determined by cell function experiments and qRT-PCR, respectively. Circ_0001947 was low-expressed in NSCLC tissues and cells. Circ_0001947 knockdown intensified cell viability and proliferation, induced cell cycle arrest at S phase, suppressed apoptosis and evidently enhanced miR-510, miR-587, miR-661 and miR-942 levels, while circ_0001947 overexpression did the opposite. MiR-661 was a target gene of circ_0001947 that participated in the regulation of circ_0001947 on cell biological behaviors. Furthermore, DOK7, the target gene of miR-661, partly participated in the regulation of miR-661 on cell viability. Hsa_circ_0001947 acts as a sponge of miR-661 to repress NSCLC development by elevating the expression of DOK7.

• 한국물환경학회지
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• 제33권6호
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• pp.661-669
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• 2017

A distributed watershed model CAMEL (Chemicals, Agricultural Management and Erosion Losses) was applied to a small rural watershed where intensive livestock farming sites are located to estimate nitrate leaching rates from soil to groundwater. The model was calibrated against the stream flows, and T-N and $NO_3-N$ concentrations were observed at the watershed outlet for three rainfall events in 2014. The simulation results showed good agreement with the observed stream flows ($R^2=0.67{\sim}0.93$), T-N concentrations ($R^2=0.40{\sim}0.58$) and $NO_3-N$ concentrations ($R^2=0.43{\sim}0.65$). The estimated annual nitrate leaching rate of the watershed was 33.0 kg N/ha/yr. The contributing proportions of individual activities to the total nitrate leaching rate of the watershed were estimated for livestock farming, applications of chemical fertilizer, and manure. The simulation results showed that the highest contributor to the nitrate leaching rate of the watershed was chemical fertilizer applications. The simulation period was for one year only, however, and results may vary depending on different conditions. Gathering input data over a longer period of time and monitoring data for calibration is needed. When this has been accomplished, it is expected that this model can be applied to small rural watersheds for evaluating temporal and spatial variations of nitrogen transformations and transport processes.

• 대한영양사협회학술지
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• 제22권2호
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• pp.131-142
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• 2016

The purpose of this study was to assess the physical activity and energy balance of weekdays and weekend in male high school students. Fifty healthy male high school students participated in this study. Anthropometric data were collected. Physical activity level (PAL) and energy intake for weekdays and weekend were calculated from a physical activity diary and food diary using the 24-hour recall method and interview. The resting metabolic rate (RMR) and estimated energy requirement (EER) were calculated from the prediction equations suggested in 2015 KDRIs. Total energy expenditure (TEE) was calculated by multiplying RMR by PAL. Mean age of subjects was $15.9{\pm}0.33years$. The daily pedometer counts were significantly higher in the weekdays (12,837 steps) than in weekend (6,661 steps) (P<0.001). The PAL of the weekdays ($1.63{\pm}0.17$) was significantly higher than that ($1.37{\pm}0.26$) of the weekend (P<0.001). PAL was significantly correlated with pedometer counts on the weekdays (r=0.495) and weekend (r=0.686). The total energy intakes ($2,847.2{\pm}681.5kcal$) and TEE ($3,046.3{\pm}437.3kcal$) of weekdays were significantly higher than those of the weekend. The results of this study would be useful to develop nutrition and exercise programs for male high school students on weekdays and weekend, respectively.

• Journal of Microbiology and Biotechnology
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• 제15권5호
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• pp.1054-1059
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• 2005

An extracellular protease was identified from Bacillus subtilis KCCM 10257 by N-terminal sequencing and mass spectral analysis. The molecular mass of the extracellular protease was estimated to be 28 kDa by SDS-PAGE. Sequencing of the N-terminal of the protease revealed the sequence of A(G,S,R)QXVPYG(A)V(P,L)SQ. The N-terminal sequence exhibited close similarity to the sequence of other proteases from Bacillus sp. A mass list of the monoisotopic peaks in the MALDI-TOF spectrum was searched after peptide fragmentation of the protease. Six peptide sequences exhibiting monoisotopic masses of 1,276.61, 1,513.67, 1,652.81, 1,661.83, 1,252.61, and 1,033.46 were observed from the fragmented protease. These monisotopic masses corresponded to the lytic enzyme L27 from Bacillus subtilis 168, and the Mowse score was found to be 75. A doubly charged Top product (MS) at a m/z of 517.3 exhibiting a molecular mass of 1034.6 was further analyzed by de novo sequencing using a PE Sciex QSTAR Hybrid Quadropole-TOF (MS/MS) mass spectrometer. MS/MS spectra of the Top product (MS) at a m/z of 517.3 obtained from the fragmented peptide mixture of protease with Q-star contained the b-ion series of 114.2, 171.2, 286.2, 357.2, 504.2, 667.4, 830.1, and 887.1 and y-ion series of 147.5, 204.2, 367.2, 530.3, 677.4, 748.4, 863.4, and 920.5. The sequence of analyzed peptide ion was identified as LGDAFYYG from the b- and y-ion series by de novo sequencing and corresponded to the results from the MALDI-TOF spectrum. From these results the extracellular protease from Bacillus subtilis KCCM 10257 was successfully identified with the lytic enzyme L27 from Bacillus subtilis 168.

• 대한소아치과학회지
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• 제35권4호
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• pp.652-661
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• 2008

본 연구는 혼합치열기 전치부 반대교합 어린이를 대상으로 FR III가 치아와 골격의 성장에 미치는 효과를 평가하고자 하였다. FR III로 처치한 전치부 반대교합을 지닌 30명의 아동(평균나이 $7.9{\pm}1.1$세 : 평균 치료기간 $1.5{\pm}0.8$년)을 대상으 로 치료 전후의 측모 두부방사선 사진을 분석하여 다음과 같은 결과를 얻었다. 1. 상악에 대한 FR III의 골격적인 효과는 상악의 하방이동이며, 전방이동은 대조군과 유의한 차이를 보이지 않았다. 2. 하악에 미치는 FR III의 골격적인 효과는 하악의 후하방이동과 SNB의 감소이다. 3. 치아에 대한 FR III의 효과는 하악 전치 첨부의 후방이동과 수평피개의 증가였다. 이상의 결과로 단기간의 FR III 치료는 상악골의 전방이동 촉진효과는 미미하며 상악골의 하방이동, 하악골의 후하방 회전, 수평피개 증가효과가 있음을 알 수 있었다.

• 동의생리병리학회지
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• 제24권4호
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• pp.661-667
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• 2010

Astragali Radix (AR) has been used in traditional medicinal herb, which is known to tonify the qi and blood. It has been asserted to be a tonic that can improve the function of the lungs, adrenal glands and the gastrointestinal tract, increase metabolism, promote healing and reduce fatigue. Also, Plantago asiatica (PA) has the anti-inflammatory, antiseptic, antitussive, cardiac, diuretic, expectorant, haemostatic effects, though it is considered to be less powerful than the seed. Recent research is showing that it is promising in lowering cholesterol and controlling diabetes. Obesity is characterized at the cellular level by an increase in the number and size of adipocytes differentiated from fibroblastic preadipocytes in adipose tissue. In the present study, we investigated the effects of 3 to 7 extracts of mixed medicinal herbs (water and ethanol extract of Astragali Radix; ARW and ARE, water and ethanol extract of Plantago asitica; PAW and PAE, and those mixed extracts; ARW+PAE, ARW+PAW and ARE+PAE) on adipogenesis and apoptosis in 3T3-L1 adipocytes by MTT assay, Oil-Red-O staining and TUNEL assay. And then, we determined total contents of phenolic compounds and flavonoid compounds in mixed herbal extracts. These result showed that herbal extracts (ARW, PAE and ARW+PAE) affected on adipogenesis and apoptosis in 3T3-L1 adipocytes among 3 to 7 extracts tested. Especially herbal mixed extracts (ARW+PAE) had the highest inhibition on differentiation of preadipocytes and lipoid accumulation of maturing adipocytes. And it did not affect cell viability of mature adipocytes, but herbal mixed extracts (ARW+PAE) increased apoptosis in mature adipocytes, as proved by highest concentration $200\;{\mu}g/m{\ell}$ using TUNEL assay. In addition, contents of total phenoilc compounds and flavonoids showed the highest level in ARE+PAE and PAE than ARW. These results suggest that this main target for adipocyte functions could be effective in improving the metabolic syndrome including obesity.

• 한국식품저장유통학회지
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• 제16권5호
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• pp.661-668
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• 2009

국내 시판 보리쌀 107개 제품을 수집하여 단백질, $\beta$-glucan 함량, 백도, 찰성비율, 취반특성 및 호화특성 등을 분석하였으며 그 결과는 다음과 같다. 국내 유통 메성보리 쌀의 단백질 함량은 6.62~11.38%였고, $\beta$-glucan 함량은 2.44~6.95%, 백도는 29.0~44.4이었으며 흡수성은 135~284%, 퍼짐성은 227~457%이었다. 찰성보리쌀의 경우 단백질은 7.17~12.57%, 백도는 27.1~49.6, $\beta$-glucan 함량은 2.81~7.38%로 메성보리쌀보다 높았으며 흡수성과 퍼짐성도 218~593%와 366~593%로 메성보리쌀보다 높았다. 즉, 찰성보리쌀 제품이 메성보리쌀 제품보다 취반특성이나 백도, $\beta$-glucan 함량 등에서 좋은 특성을 나타내었다. 호화특성은 지역별 생산제품별로 차이가 있었으며 호화개시온도는 메성보리쌀 제품이 찰성보리쌀 제품보다 높았고 최고점도, 최저점도, 최종점도들도 메성보리쌀 제품이 찰성보리쌀 제품보다 높게 나타났다. 메성과 찰성에 관계없이 단백질 함량과 $\beta$-glucan 함량은 정의 상관관계($r=0.632^{***}$, $r=0.453^{**}$)를 나타내었으며, 백도와는 부의 상관관계($r=-0.433^{***}$, $r=-0.343^{**}$)를 나타내었다. 찰성보리쌀 제품의 찰성비율을 분석한 결과 84~100% 범위였으며, 평균 찰성비율은 97%이었고 이중 16개 제품이 98%이상의 찰성비율을 나타내는 등 시중에 판매되고 있는 제품들의 찰성비율이 지역별, 제품별로 큰 차이를 보였다.