• Journal of Microbiology and Biotechnology
• /
• v.31 no.11
• /
• pp.1508-1518
• /
• 2021

Hsa_circ_0001947 is associated with multiple cancers, but its function in non-small cell lung cancer (NSCLC) is ambiguous and needs further research. The targeting relationship among circ_0001947, miR-661, and downstream of tyrosine kinase 7 (DOK7) was predicted by database and further verified by dual-luciferase reporter assay, while their expressions in cancer tissues and cells were detected by quantitative real-time polymerase chain reaction (qRT-PCR). After transfection, cell biological behaviors and expressions of miRNAs, miR-661 and DOK7 were determined by cell function experiments and qRT-PCR, respectively. Circ_0001947 was low-expressed in NSCLC tissues and cells. Circ_0001947 knockdown intensified cell viability and proliferation, induced cell cycle arrest at S phase, suppressed apoptosis and evidently enhanced miR-510, miR-587, miR-661 and miR-942 levels, while circ_0001947 overexpression did the opposite. MiR-661 was a target gene of circ_0001947 that participated in the regulation of circ_0001947 on cell biological behaviors. Furthermore, DOK7, the target gene of miR-661, partly participated in the regulation of miR-661 on cell viability. Hsa_circ_0001947 acts as a sponge of miR-661 to repress NSCLC development by elevating the expression of DOK7.

• Journal of Korean Society on Water Environment
• /
• v.33 no.6
• /
• pp.661-669
• /
• 2017

A distributed watershed model CAMEL (Chemicals, Agricultural Management and Erosion Losses) was applied to a small rural watershed where intensive livestock farming sites are located to estimate nitrate leaching rates from soil to groundwater. The model was calibrated against the stream flows, and T-N and $NO_3-N$ concentrations were observed at the watershed outlet for three rainfall events in 2014. The simulation results showed good agreement with the observed stream flows ($R^2=0.67{\sim}0.93$), T-N concentrations ($R^2=0.40{\sim}0.58$) and $NO_3-N$ concentrations ($R^2=0.43{\sim}0.65$). The estimated annual nitrate leaching rate of the watershed was 33.0 kg N/ha/yr. The contributing proportions of individual activities to the total nitrate leaching rate of the watershed were estimated for livestock farming, applications of chemical fertilizer, and manure. The simulation results showed that the highest contributor to the nitrate leaching rate of the watershed was chemical fertilizer applications. The simulation period was for one year only, however, and results may vary depending on different conditions. Gathering input data over a longer period of time and monitoring data for calibration is needed. When this has been accomplished, it is expected that this model can be applied to small rural watersheds for evaluating temporal and spatial variations of nitrogen transformations and transport processes.

• Journal of the Korean Dietetic Association
• /
• v.22 no.2
• /
• pp.131-142
• /
• 2016

The purpose of this study was to assess the physical activity and energy balance of weekdays and weekend in male high school students. Fifty healthy male high school students participated in this study. Anthropometric data were collected. Physical activity level (PAL) and energy intake for weekdays and weekend were calculated from a physical activity diary and food diary using the 24-hour recall method and interview. The resting metabolic rate (RMR) and estimated energy requirement (EER) were calculated from the prediction equations suggested in 2015 KDRIs. Total energy expenditure (TEE) was calculated by multiplying RMR by PAL. Mean age of subjects was $15.9{\pm}0.33years$. The daily pedometer counts were significantly higher in the weekdays (12,837 steps) than in weekend (6,661 steps) (P<0.001). The PAL of the weekdays ($1.63{\pm}0.17$) was significantly higher than that ($1.37{\pm}0.26$) of the weekend (P<0.001). PAL was significantly correlated with pedometer counts on the weekdays (r=0.495) and weekend (r=0.686). The total energy intakes ($2,847.2{\pm}681.5kcal$) and TEE ($3,046.3{\pm}437.3kcal$) of weekdays were significantly higher than those of the weekend. The results of this study would be useful to develop nutrition and exercise programs for male high school students on weekdays and weekend, respectively.

• Journal of Microbiology and Biotechnology
• /
• v.15 no.5
• /
• pp.1054-1059
• /
• 2005

An extracellular protease was identified from Bacillus subtilis KCCM 10257 by N-terminal sequencing and mass spectral analysis. The molecular mass of the extracellular protease was estimated to be 28 kDa by SDS-PAGE. Sequencing of the N-terminal of the protease revealed the sequence of A(G,S,R)QXVPYG(A)V(P,L)SQ. The N-terminal sequence exhibited close similarity to the sequence of other proteases from Bacillus sp. A mass list of the monoisotopic peaks in the MALDI-TOF spectrum was searched after peptide fragmentation of the protease. Six peptide sequences exhibiting monoisotopic masses of 1,276.61, 1,513.67, 1,652.81, 1,661.83, 1,252.61, and 1,033.46 were observed from the fragmented protease. These monisotopic masses corresponded to the lytic enzyme L27 from Bacillus subtilis 168, and the Mowse score was found to be 75. A doubly charged Top product (MS) at a m/z of 517.3 exhibiting a molecular mass of 1034.6 was further analyzed by de novo sequencing using a PE Sciex QSTAR Hybrid Quadropole-TOF (MS/MS) mass spectrometer. MS/MS spectra of the Top product (MS) at a m/z of 517.3 obtained from the fragmented peptide mixture of protease with Q-star contained the b-ion series of 114.2, 171.2, 286.2, 357.2, 504.2, 667.4, 830.1, and 887.1 and y-ion series of 147.5, 204.2, 367.2, 530.3, 677.4, 748.4, 863.4, and 920.5. The sequence of analyzed peptide ion was identified as LGDAFYYG from the b- and y-ion series by de novo sequencing and corresponded to the results from the MALDI-TOF spectrum. From these results the extracellular protease from Bacillus subtilis KCCM 10257 was successfully identified with the lytic enzyme L27 from Bacillus subtilis 168.

• Journal of the korean academy of Pediatric Dentistry
• /
• v.35 no.4
• /
• pp.652-661
• /
• 2008

The purpose of this study was to evaluate the skeletal and dental effects obtained by the Frankel functional regulator III in growing children with Class III malocclusions. Cephalometric changes in thirty children at the time of mixed dentition malocclusions (initial mean age, $7.9{\pm}1.1$ years; mean treatment duration, $1.5{\pm}0.8$ years) were analysed. The results were as follows : 1. The skeletal effects on the maxilla showed a significant downward displacement whereas forward displacement was not significant in comparison with the control group. 2. The skeletal effects on the mandible showed statistically significant backward and downward displacement. 3. The dental effects showed statistically significant backward movement in the mandibular incisor tip and increase of overjet The results suggested that forward displacement on the maxilla was insufficient and treatment effects were caused mainly by downward displacement of the maxilla, backward and downward rotation of the mandible, and the increase of overjet during short period.

• Journal of Physiology & Pathology in Korean Medicine
• /
• v.24 no.4
• /
• pp.661-667
• /
• 2010

Astragali Radix (AR) has been used in traditional medicinal herb, which is known to tonify the qi and blood. It has been asserted to be a tonic that can improve the function of the lungs, adrenal glands and the gastrointestinal tract, increase metabolism, promote healing and reduce fatigue. Also, Plantago asiatica (PA) has the anti-inflammatory, antiseptic, antitussive, cardiac, diuretic, expectorant, haemostatic effects, though it is considered to be less powerful than the seed. Recent research is showing that it is promising in lowering cholesterol and controlling diabetes. Obesity is characterized at the cellular level by an increase in the number and size of adipocytes differentiated from fibroblastic preadipocytes in adipose tissue. In the present study, we investigated the effects of 3 to 7 extracts of mixed medicinal herbs (water and ethanol extract of Astragali Radix; ARW and ARE, water and ethanol extract of Plantago asitica; PAW and PAE, and those mixed extracts; ARW+PAE, ARW+PAW and ARE+PAE) on adipogenesis and apoptosis in 3T3-L1 adipocytes by MTT assay, Oil-Red-O staining and TUNEL assay. And then, we determined total contents of phenolic compounds and flavonoid compounds in mixed herbal extracts. These result showed that herbal extracts (ARW, PAE and ARW+PAE) affected on adipogenesis and apoptosis in 3T3-L1 adipocytes among 3 to 7 extracts tested. Especially herbal mixed extracts (ARW+PAE) had the highest inhibition on differentiation of preadipocytes and lipoid accumulation of maturing adipocytes. And it did not affect cell viability of mature adipocytes, but herbal mixed extracts (ARW+PAE) increased apoptosis in mature adipocytes, as proved by highest concentration $200\;{\mu}g/m{\ell}$ using TUNEL assay. In addition, contents of total phenoilc compounds and flavonoids showed the highest level in ARE+PAE and PAE than ARW. These results suggest that this main target for adipocyte functions could be effective in improving the metabolic syndrome including obesity.

• Food Science and Preservation
• /
• v.16 no.5
• /
• pp.661-668
• /
• 2009

A total of 107 pearled barley products produced in Korea, 58 non-waxy and 49 waxy, were analyzed for protein and $\beta$-glucan content, whiteness, cooking characteristics (water absorption and expansibility), and pasting properties, with respect to the region of production. We compared non-waxy and waxy pearled barley products and sought correlations between levels of chemical components and cooking characteristics. Waxy pearled barley products had higher concentrations of protein (7.17-12.57%, w/w) and $\beta$-glucan (2.81-7.38%, w/w), a higher whiteness grade (27.1-49.6), and a greater water absorption (218-593%) and expansibility (366-593%) than did non-waxy barley products. The pasting temperature of non-waxy pearled barley ($73.9^{\circ}C$) was higher than that of waxy pearled barley ($66.9^{\circ}C$). Peak viscosity, hot paste viscosity, and final viscosity of non-waxy pearled barley products were higher than those of waxy products. The results showed that waxy pearled barley products had better cooking characteristics than did non-waxy products. A significant positive correlation was observed between protein and $\beta$-glucan content in both non-waxy and waxy pearled barley products ($r=0.632^{***}$ and $r=0.453^{**}$, respectively). Whiteness showed a negative correlation with protein content of both non-waxy and waxy pearled barley products ($r=-0.433^{***}$, $r=-0.343^{**}$). However, neither water absorption nor expansibility showed any significant correlation with protein or $\beta$-glucan content. The waxy ratio of 49 waxy pearled barley products ranged from 84-100%.