• 제목/요약/키워드: miR-29a

검색결과 226건 처리시간 0.027초

Anti-Aging Activity of Lavandula angustifolia Extract Fermented with Pediococcus pentosaceus DK1 Isolated from Diospyros kaki Fruit in UVB-Irradiated Human Skin Fibroblasts and Analysis of Principal Components

  • Ha, Ji Hoon;Kim, A Rang;Lee, Keon-Soo;Xuan, Song Hua;Kang, Hee Cheol;Lee, Dong Hwan;Cha, Mi Yeon;Kim, Hye Jin;An, Mi;Park, Soo Nam
    • Journal of Microbiology and Biotechnology
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    • 제29권1호
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    • pp.21-29
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    • 2019
  • The effects of Lavandula angustifolia extract fermented with Pediococcus pentosaceus DK1 on UVB-mediated MMP-1 expression and collagen decrease in human skin fibroblasts were determined, and the conversion of its components was also analyzed. Fermentation was performed at varying L. angustifolia extract and MRS medium concentrations, and optimal fermentation conditions were selected. L. angustifolia extracts showed decreased cytotoxicity after fermentation in the fibroblasts. UVB-irradiated fibroblasts treated with fermented L. angustifolia extract showed MMP-1 expression 8.2-14.0% lower than that in UVB-irradiated fibroblasts treated with non-fermented extract. This was observed even at fermented extract concentrations lower than those of non-fermented extracts. Fibroblasts treated with fermented L. angustifolia extract showed 20% less reduction in collagen production upon UVB irradiation than those treated with non-fermented extracts. UVB-irradiated fibroblasts treated with fermented L. angustifolia extracts showed 50% higher inhibition of ROS generation than those treated with non-fermented extract. Luteolin and apigenin glycosides of L. angustifolia were converted during fermentation, and identified using RP-HPLC and LC/ESI-MS. Therefore, the effects of L. angustifolia extract on MMP-1 expression and collagen decrease in UVB-irradiated human skin fibroblasts were increased through fermentation by P. pentosaceus.

강인성이 건강증진행위, 신체증상에 미치는 효과-여대생을 대상으로- (Hardiness as a Stress-resistance Resource and as a Determinant of Health -promoting Behavior: in a Sample of Nursing Students)

  • 이미라;소희영;송영신
    • 지역사회간호학회지
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    • 제9권1호
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    • pp.21-29
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    • 1998
  • This study was undertaken in order to examine the relationship of hardiness and health-promoting behavior and the effects of hardiness on stress-related physical symptoms. The subjects were 104 female nursing students of one college in Kongju. The instruments used for this study were a survey of general characteristics, hardiness(25 items), health-promoting behavior(44 items), and physical symptoms(35 items), Analysis of data was done by use of mean, Pearson correlation coefficient, stepwise regression and a hierarchical multiple regression with an SAS program. The results of this study are as follows. 1) Significant correlations between health -promoting behavior and subscales of hardiness, that is, control(r=-.35, P<.00l), commitment(r= -.29, P<.0l), and challenge(r= -.23, p<.05) were found. 2) Control was the highest factor predicting health -promoting behavior. 3) Main and buffering effects of hardiness on current physical symptoms were not found.

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Report of 29 unrecorded bacterial species from the phylum Proteobacteria

  • Nam, Yoon-Jong;Beak, Kiwoon;Han, Ji-Hye;Park, Sanghwa;Lee, Mi-Hwa
    • Journal of Species Research
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    • 제7권1호
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    • pp.60-72
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    • 2018
  • Our study aimed to discover indigenous prokaryotic species in Korea. A total of 29 bacterial species in the phylum Proteobacteria were isolated from freshwater and sediment of rivers and brackish zones in Korea. From the high 16S rRNA gene sequence similarity (${\geq}98.8%$) and formation of a robust phylogenetic clade with the closest species, it was determined that each strain belonged to an independent and predefined bacterial species. To our knowledge, there is no official report or publication that has previously described these 29 species in Korea. Specifically, we identified 10, 12, and seven species of eight, 12, and seven genera that belong to classes Alphaproteobacteria, Betaproteobacteria, and Gammaproteobacteria, respectively; all are reported as previously unrecorded bacterial species in Korea. The Gram reaction, colony and cell morphology, basic biochemical characteristics, isolation source, and strain IDs for each are also described.

Nitric Oxide and Hydrogen Peroxide Production are Involved in Systemic Drought Tolerance Induced by 2R,3R-Butanediol in Arabidopsis thaliana

  • Cho, Song-Mi;Kim, Yong Hwan;Anderson, Anne J.;Kim, Young Cheol
    • The Plant Pathology Journal
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    • 제29권4호
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    • pp.427-434
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    • 2013
  • 2R,3R-Butanediol, a volatile compound produced by certain rhizobacteria, is involved in induced drought tolerance in Arabidopsis thaliana through mechanisms involving stomatal closure. In this study, we examined the involvement of nitric oxide and hydrogen peroxide in induced drought tolerance, because these are signaling agents in drought stress responses mediated by abscisic acid (ABA). Fluorescence-based assays showed that systemic nitric oxide and hydrogen peroxide production was induced by 2R,3R-butanediol and correlated with expression of genes encoding nitrate reductase and nitric oxide synthase. Co-treatment of 2R,3R-butanediol with an inhibitor of nitrate reductase or an inhibitor of nitric oxide synthase lowered nitric oxide production and lessened induced drought tolerance. Increases in hydrogen peroxide were negated by co-treatment of 2R,3R-butanediol with inhibitors of NADPH oxidase, or peroxidase. These findings support the volatile 2R,3R-butanediol synthesized by certain rhizobacteria is an active player in induction of drought tolerance through mechanisms involving nitric oxide and hydrogen peroxide production.

요양보호사의 응급처치 수행자신감 예측요인 (Predictors of Caregivers' First Aid Confidence)

  • 김순옥;김미희
    • 한국응용과학기술학회지
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    • 제40권4호
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    • pp.811-824
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    • 2023
  • 본 연구는 요양보호사를 대상으로 의사소통 자신감, 자기효능감, 응급처치 수행자신감 정도와 상관관계를 확인하고 응급처치 수행자신감 예측요인을 알아보고자 실시하였다. 요양보호사 202명을 대상으로 2022년 3월 1일부터 3월 31일까지 자료수집 하였으며, 수집된 자료는 SPSS 29.0 프로그램을 이용하여 t-test, ANOVA, Scheffe's test, Person's correlation coefficients, Multiple linear regression으로 분석하였다. 자기효능감은 의사소통 자신감과 정적 상관관계(r=.54, p<.001), 응급처치 수행자신감은 의사소통 자신감(r=-.18, p<.05), 자기효능감과 부적 상관관계를 보였다(r=-.31, p<.001). 응급처치 수행자신감 예측요인으로 간호조무사가 없을 때(β=-.18, p=.009)와 자기효능감(β=-.30, p<.001)으로 나타났고, 설명력은 11.0%였다(Adj R2=.110, p<.001). 본 연구결과를 근거로 요양보호사의 응급처치 수행자신감 향상을 위한 응급교육 프로그램을 개발하여 적용할 것을 제언한다.

화상환자의 소양증 특성 조사 (Pruritus in Burn Survivors)

  • 김경자;원미화
    • 한국산학기술학회논문지
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    • 제17권1호
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    • pp.45-53
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    • 2016
  • 본 연구 목적은 화상환자의 소양증 특성을 파악하기 위한 서술적 조사 연구로 화상환자 소양증 기초자료 제공을 위함이다. 심부 2도 이상 화상으로 입원 중인 급성기 화상환자 95명을 대상으로 자기보고식 설문지를 이용하여 화상관련 특성과 소양증 관련 특성을 측정하였다. 연구결과 대상자의 화상범위는 평균 $18.95{\pm}17.10%$였으며, 소양증 중증도 평균 $48.83{\pm}23.96$점, 증상고통 평균 $46.42{\pm}25.80$점으로 나타났다. 소양증 빈도에 따른 결과는 '항상' 경험할 때 그렇지 않은 경우보다 잠들기 어려움(F=4.817, p=.004), 기분 나빠짐(F=5.955, p<.001), 집중력 저하(F=6.483, p<.001)에서 유의한 차이를 보였고, 지속시간에 따른 소양증 결과는 30분 이상 지속 시 30분 이내보다, 잠들기 어려움(F=7.452, p<.001), 기분 나빠짐(F=3.655, p=.015), 집중력 저하(F=12.564, p<.001)에서 통계적으로 유의한 차이가 있었다. 화상환자의 소양증 빈도는 지속시간(r=.29, p=.004), 중증도(r=.45, p<.001), 증상고통(r=.44, p<.001) 및 소양증 결과(r=.29, p=.004)와 양의 상관관계를 보였다. 본 연구 결과는 소양증을 경험하는 화상환자의 간호중재를 위한 근거자료로 활용 될 수 있을 것이다.

Factors Influencing Preventive Behaviors for COVID-19 in Undergraduate Nursing Students: A Cross-Sectional Descriptive Study

  • Choi, Jeong Sil;Kim, Kyung Mi
    • 대한간호학회지
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    • 제52권6호
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    • pp.554-563
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    • 2022
  • Purpose: This study aimed to determine how undergraduate nursing students' knowledge and health beliefs affected their COVID-19-related infection-prevention behaviors. Methods: This study used a descriptive survey. A total of 188 undergraduate nursing students from two universities in South Korea participated in this study. The data were collected from June 2020 to August 2020. Factors influencing infection-prevention behaviors were identified using multiple regression analysis. Results: The participants' mean knowledge level regarding COVID-19 was 84.05 ± 11.78 out of 100. The average health belief score was 2.80 ± 0.32 points out of 5. COVID-19-related preventive health behaviors were correlated with experiences of searching for COVID-19 information (r = .22, p < .01), perceived severity (r = .24, p < .01), perceived benefits (r = .29, p < .01), cues to action (r = .30, p < .01), knowledge (r = .27, p < .01), and perceived barriers (r = - .19, p < .05). Factors that significantly affected COVID-19-related preventive health behaviors were the participants' years of study, experiences regarding COVID-19 prevention education, perceived severity, perceived barriers, and cues to action. Conclusion: COVID-19-related preventive health behaviors are promoted by increasing awareness about the disease and promoting COVID-19 education in nursing curriculums.

Rapid Identification of Diaporthe citri by Gene Sequence Analysis

  • Zar Zar Soe;Yong Ho Shin;Hyun Su Kang;Mi Jin Kim;Yong Chull Jeun
    • 식물병연구
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    • 제29권2호
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    • pp.130-136
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    • 2023
  • Citrus melanoses caused by Diaporthe citri, has been one of the serious diseases in many citrus orchards of Jeju Island. To protect melanose in citrus farms, a fast and exact diagnosis method is necessary. In this study, diseased leaves and dieback twigs were collected from a total of 49 farms within March to April in 2022. A total of 465 fungal isolates were obtained from a total of 358 isolated plant samples. Among these fungal isolates, 40 representatives of D. citri isolates which were isolated from 22 twigs and 18 leaves on 23 farms were found based on cultural characteristics on potato dextrose agar and conidial morphology. Additionally, the molecular assay was carried out and compared with those by morphological diagnosis. All isolates were identified as D. citri by analyzing the sequences at the internal transcribed spacer (ITS) rDNA region using primers of ITS1/ITS4 or at β-tubulin using primer Btdcitri-F/R. Therefore, based on the present study, where the results of morphological identification of conidial type were consistent with DNA sequence analysis of certain gene, choosing a suitable method for a fast diagnosis of citrus melanose was suggested.

일 지역사회 산업장 근로자의 음주형태, 직무스트레스 및 여가만족도가 건강증진행위에 미치는 영향 (The Effects of Drinking Patterns, Job Stress, and Leisure Satisfaction on Health Promotion Behaviors of Industrial Workers)

  • 장경오;박미화
    • 한국산학기술학회논문지
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    • 제22권3호
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    • pp.499-510
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    • 2021
  • 본 연구는 산업장 근로자의 음주형태, 직무스트레스 및 여가만족도가 건강증진 행위에 미치는 영향을 파악하기 위한 서술적 조사연구이다. 조사대상은 U광역시에 소재한 3개의 병원에서 건강검진을 받는 산업장 근로자 207명을 대상으로 시행하였으며, 구조화된 설문지를 통해 2019년 11월 1일부터 2019년 11월 30일까지 자료를 수집하였다. 자료 분석은 IBM SPSS 24.0 통계프로그램으로 분석하였다. 그 결과, 대상자의 음주빈도는 주 2~3회 음주가 36.3%로 가장 많았고, 술을 마시는 동기는 친목 도모가 54.6%이었다. 직무스트레스의 평균 점수는 62.46±5.59점, 여가만족도는 30.29±6.39점, 건강증진행위는 125.64±8.77점으로 중간보다 다소 높았다. 연구대상자의 건강증진행위는 연령(r=-.162, p<.020)과 음의 상관관계, 결혼상태(r=.149, p<.032), 직무스트레스(r=.227, p<.001) 여가만족도(r=.542, p<.001)는 유의한 양의 상관관계가 있는 것으로 나타났다. 연구대상자의 건강증진행위에 영향을 미치는 요인을 다중회귀로 분석한 결과, 음주형태, 직무스트레스 및 여가 만족도가 건강증진행위에 영향을 미치는 요인으로 설명력은 29.7%이었다. 본 연구를 기반으로 산업장 근로자들이 음주가 아닌 여가활동을 통한 친목 도모와 건강증진을 위한 다양한 여가활동으로 직무스트레스에서 벗어날 수 있도록 지속적인 지원이 필요하며, 이를 위한 프로그램운영과 시스템 지원으로 산업장 근로자들이 건강한 생활양식과 건강증진 활동을 통한 삶의 질 개선을 기대한다.