• Title/Summary/Keyword: miR-27a

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Isolation and Characterization of a Protease-Producing Bacterium, Bacillus amyloliquefaciens P27 from Meju as a Probiotic Starter for Fermented Meat Products

  • Lee, Mi-Sun;Lee, Na-Kyoung;Chang, Kyung-Hoon;Choi, Shin-Yang;Song, Chi-Kwang;Paik, Hyun-Dong
    • Food Science of Animal Resources
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    • v.30 no.5
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    • pp.804-810
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    • 2010
  • This study was performed to select protease-producing Bacillus sp. as a potential probiotic starter for fermented meat products. In order to isolate protease-producing bacterium from meju, measured the diameter of the clear zone on agar plate (TSA, 1% (w/v) skim milk) and analyzed for intracellular protease activity, then 10 Bacillus-like strains were isolated. Three Bacillus-like strains (P19, P27, and P33) among 10 strains were able to tolerate in acidic condition (TSB, pH 2.5, 2 h incubation). These 3 strains were showed antimicrobial activity against food-borne pathogenic bacteria. These vegetative cells of 3 strains were showed a survival rate of 0.04% to 0.08% under the artificial gastric acidic condition (TSB, pH 2.5 with 1% (w/v) pepsin), but spore-forming cells were 56.29% to 84.77%. Vegetative cells of 3 strains were the least bile-resistant, while spore-forming cells of 3 strains showed higher survival rate more than 76% under artificial bile condition (TSB, 0.1% (w/v) oxgall bile). In these strains, P27 strain was finally selected as a good probiotic strain. P27 strain was tentatively identified as Bacillus amyloliquefaciens by API CHB kit and 16S rDNA sequence analysis. The results of this study suggest that B. amyloliquefaciens P27 can be used as a potential probiotic starter for fermented meat product.

Evaluation of Vi-Passive Hemagglutination, SD$^{(R)}$ Kit, and Widal Test for Serological Diagnosis of Typhoid Fever (장티푸스의 혈청학적 진단을 위한 Vi-수동혈구응집법, SD$^{(R)}$ Kit 및 Widal 시험에 대한 효용성 평가)

  • Kim, Sung-Hun;Kim, Shuk-Ho;Lee, Deog-Yong;Lee, Esther;Park, Mi-Sun;Lee, Bok-Kwon
    • Korean Journal of Microbiology
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    • v.46 no.2
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    • pp.219-222
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    • 2010
  • In this study, we evaluated Vi-passive hemagglutination (Vi-PHA), SD Salmonella Typhi IgG/IgM ($SD^{(R)}$ kit) and Widal test for the rapid laboratory diagnosis of typhoid fever patients. A total of 36 serum samples from febrile patients in Korea from 2005 to 2006 were used. Among 36 patients, 27 were fever patients without typhoid, 9 were typhoid fever. Vi-PHA showed 8 positive results out of 9 typhoid fever patients (sensitivity 88.9%) and 1 positive and 26 negative results out of 27 febrile patients without typhoid (specificity 96.3%). The sensitivity and the specificity of $SD^{(R)}$ kit were 100% and 92.6%, respectively. However, the sensitivity and the specificity of Widal O & H tests were 88.9%, 100%, and 77.8%, 70.4%, respectively. Consequently, Widal H and $SD^{(R)}$ kit showed higher sensitivity and Vi-PHA showed higher specificity. To efficient diagnosis, Vi-PHA may be sufficient diagnosis method in acute cases and $SD^{(R)}$ kit and Widal test may be sufficient in sporadic area and high risk group.

Factors Influencing Preventive Behaviors for COVID-19 in Undergraduate Nursing Students: A Cross-Sectional Descriptive Study

  • Choi, Jeong Sil;Kim, Kyung Mi
    • Journal of Korean Academy of Nursing
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    • v.52 no.6
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    • pp.554-563
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    • 2022
  • Purpose: This study aimed to determine how undergraduate nursing students' knowledge and health beliefs affected their COVID-19-related infection-prevention behaviors. Methods: This study used a descriptive survey. A total of 188 undergraduate nursing students from two universities in South Korea participated in this study. The data were collected from June 2020 to August 2020. Factors influencing infection-prevention behaviors were identified using multiple regression analysis. Results: The participants' mean knowledge level regarding COVID-19 was 84.05 ± 11.78 out of 100. The average health belief score was 2.80 ± 0.32 points out of 5. COVID-19-related preventive health behaviors were correlated with experiences of searching for COVID-19 information (r = .22, p < .01), perceived severity (r = .24, p < .01), perceived benefits (r = .29, p < .01), cues to action (r = .30, p < .01), knowledge (r = .27, p < .01), and perceived barriers (r = - .19, p < .05). Factors that significantly affected COVID-19-related preventive health behaviors were the participants' years of study, experiences regarding COVID-19 prevention education, perceived severity, perceived barriers, and cues to action. Conclusion: COVID-19-related preventive health behaviors are promoted by increasing awareness about the disease and promoting COVID-19 education in nursing curriculums.

The Influence of Spirituality, Meaning in Life and Hospice Perception on Attitudes towards Terminal Care in Nursing Students (간호대학생의 영성, 생의 의미, 호스피스 인식 정도가 임종간호태도에 미치는 영향)

  • Sim, Mi-Kyung;Park, Jeong-Mo
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.18 no.7
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    • pp.290-299
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    • 2017
  • This study was conducted to evaluate the spirituality, perceived meaning in life, hospice perception, and attitudes towards terminal care among nursing students, as well as to identify the relationships between variables and examine factors influencing their attitudes towards terminal care. Data were collected using a self-reported questionnaire completed by 308 nursing students at two universities from April 11, 2017 to April 17, 2017. Data were analyzed using descriptive analysis, a t-test, Pearson's correlation coefficients and stepwise multiple regression with the SPSS/Win 14.0 program. Attitudes toward terminal care among nursing students were significantly correlated with hospice perception(r=0.30, p<0.001) and meaning in life(r=0.30, p<0.001). Meaning in life among nursing students was significantly correlated with hospice perception(r=0.25, p<0.01) and spirituality(r=0.53, p<0.01). The significant predictors of the attitudes towards terminal care in nursing students were hospice perception(${\beta}=0.40$, p<0.001) and meaning in life(${\beta}=0.27$, p<0.001). Hospice perception explained 22.7% and meaning in life explained 5.0% of the variance. These variables explained 27.7% of the variance in attitudes toward terminal care. Overall, these findings suggest that the terminal care education program, including hospice perception and meaning in life, is necessary to enhance the positive attitude toward terminal care among nursing students.

Factors Influencing the Retention Intention of Nurses in General Hospital Nurses (종합병원 간호사의 재직의도에 영향을 미치는 요인)

  • Kim, Yu-Mi;Seo, Minjeong
    • Journal of Digital Convergence
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    • v.18 no.12
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    • pp.377-387
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    • 2020
  • The purpose of this study is a descriptive research study to identify the emotional labor, ethical climate, job satisfaction, and retention intention of nurses in general hospitals, and to identify the relationship between variables and factors influencing the retention intention. The study subjects were 198 nurses working in four general hospitals, and data were collected from September 18 to September 30, 2019 using a structured questionnaire. The collected data were analyzed by t-test, ANOVA, Pearson's correlation coefficients, and multiple regression using SPSS WIN 25.0. As a result of the study, there was a positive correlation between ethical climate(r=.345, p<.001) and job satisfaction(r=.388, p<.001), but emotional labor (r=-.153, p=.031) showed negative correlation. Factors influencing the retention intention of general hospital nurses are job satisfaction(β=.27, p=.001), age(β=.27, p<001), ethical climate(β=.16, p=044), and the explanatory power of the variable was 24.2%. In order to increase the retention intention of general hospital nurses, it is necessary to increase job satisfaction and create a sound ethical climate, and to develop an intervention program that can increase the retention intention by age.

Factors affecting the attitude toward withdrawal of life-sustaining treatment of nurses working at long-term care hospitals (요양병원 간호사의 연명치료중단에 대한 태도에 미치는 영향 요인)

  • Kim, Mi Sook
    • The Journal of Korean Academic Society of Nursing Education
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    • v.26 no.4
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    • pp.383-392
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    • 2020
  • Purpose: This study was a descriptive study to identify factors affecting the attitude toward life-sustaining treatment of nurses working at long-term care hospitals. Methods: Data were collected through structured questionnaires from August 2nd to 27th, 2019. Study participants consisted of 163 nurses who were working for at least 6 months from 7 long-term care hospitals in B and K city. Data were analyzed using t-test, ANOVA, Scheffe' test, Pearson's correlation coefficients, and hierarchical regression with SPSS WIN v 21.0. Results: There were significantly positive correlations between awareness of good death (r=.46, p<.001) and perception of patients' rights (r=.32, p<.001). The factors affecting participants' attitude toward life-sustaining treatment were awareness of good death (β=.35, p<.001) and their own view of death (β=.24, p=.001), which explained about 27.0% of the attitude toward life-sustaining treatment. Conclusion: Based on these results, it is necessary to develop nursing educational materials that can establish values for deaths, and cultivate legal and ethical knowledge related to attitude toward life-sustaining treatment. In addition, since the severity of a patient's condition varies and the characteristics of the institution vary depending on the type of hospital, a study is needed on the relevance of variables considering the hospital environment.

The Effect of Depression, Life Stress and Resilience on Quality of Life in Middle Aged Women (중년여성의 우울, 생활스트레스 및 회복탄력성이 삶의 질에 미치는 영향)

  • Yang, Kyoung Mi
    • Journal of Korean Academic Society of Home Health Care Nursing
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    • v.22 no.2
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    • pp.300-309
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    • 2015
  • Purpose: This study investigated the effect of depression, life stress and resilience on quality of life among middle-aged women. Methods: A descriptive correlation study design was used with a convenience sample consisting of 201 middle-aged women recruited from an urban area. SPSS 21.0 was used for a descriptive analysis, t-test, ANOVA, Pearson's correlations and multiple-regression analysis. Results: Quality of life had a significant positive correlation with resilience(r=.53, p<.001) and negative correlation with life stress(r=-.27, p<.001). The significant factors for quality of life were resilience (B=.425, p<.001) marital-status (B=.227, p=.001) and health status (B=-216, p=.001). These factors explained 36.8% of the variance in quality of life. Conclusions: In conclusion, to promote quality of life middle-aged women, there is a need to develop an intervention program to improve resilience.

The Relationship between Stress Coping, Spouse Support, Intentional Rumination and Post-traumatic Growth in Women with Hysterectomy (자궁 절제술을 경험한 여성의 스트레스 대처, 배우자지지, 의도적 반추와 외상 후 성장의 관계)

  • Chae, Yeo-Joo;Kim, Young-Ran;Lee, Ok-Suk;Kim, Bo-Ram;Ko, Young-Shim;Han, Sang-Mi;Ha, Yeong-Mi
    • Journal of the Korea Convergence Society
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    • v.11 no.8
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    • pp.237-245
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    • 2020
  • The purpose of this study was to identify the relationship between stress coping, spouse support, intentional rumination, and post-traumatic growth of subjects who experienced traumatic events hysterectomy. The subjects collected from 67 women who had hysterectomy were analyzed using t-test, ANOVA and multiple regression analysis. As a result of this study, the post-traumatic growth of the subjects was passive coping (r=0.27, p=.026), active coping (r=0.34, p=.004), and intentional reflection (r=0.46, p<.001)), and passive coping was positively correlated with active coping (r=0.74, p<.001). Regression analysis to identify factors affecting on the post-traumatic growth of women with hysterectomy showed that intentional rumination was the significant factor(β = 0.42, p <.001). and their total explanatory power was 26% (F = 8.68, p < .001). Therefore, results of this study, in order to promote post-traumatic growth of subjects with hysterectomy, an intervention program is needed to increase the intentional rumination to effectively face the trauma they suffer.

Factors Influencing Work-related Burnout of Nurses and Caregivers in Nursing Homes (노인요양시설 간호사와 요양보호사의 직무관련 소진 영향요인)

  • Lee, Young-Mi;Kim, Sung-Ja;Kim, Kisook
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.14 no.4
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    • pp.1755-1764
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    • 2013
  • This study was to identify factors influencing the job-related burnout of the nurses and caregivers in nursing homes. The participants in this study were 188 who were working as nurses (77) and caregivers (111) at five nursing homes in Seoul and Gyeonggi Province. Collected data were analyzed in independent t-test, Pearson's correlation coefficients, multiple stepwise regression using IBM SPSS Statistics 19.0. The nurse's job-related burnout was positively correlated with job-satisfaction(r=.333, p=.003), and stress(r=.484, p<.001), whereas caregiver's job-related burnout was positively correlated with stress(r=.458, p<.001). The factor influencing on nurse's job-related burnout was interpersonal relation stress and this factors explained 27.0% of the variance. In case of the caregivers, work-related stress was a factor influencing on job-related burnout and it explained 21.2% of the variance. On the basis of the results in this study, the improvement of working conditions, and the development of stress management program should be considered systematically.

The Influence of Clinical Nurses' Sleep Disorder, Organizational Support, Health promotion behaviors on Burn-out (간호사의 수면장애, 조직 내 지지, 건강증진행위가 소진에 미치는 영향)

  • Jang, Jin Suk;Kim, Mi Young
    • The Journal of the Convergence on Culture Technology
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    • v.7 no.2
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    • pp.27-33
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    • 2021
  • The purpose of this study was to investigate the influence of clinical nurses' sleep disorder, organizational support, and health promotion behaviors on professional quality of life. The subjects were 256 nurses working in hospitals in more than 200 and less than 300 beds hospital in B and G cities. The data were collected from September 4, 2017 to September 11, 2017. The collected data were analyzed by frequency analysis t-test, ANOVA, Scheffé test, Pearson's correlation coefficients, and multiple regression analysis using SPSS WIN 20.0. Burnout rate was 28.90± 4.00, burnout was associated with sleep disorder(r=-.321, p<.001), organizational support(r=-.321, p<.001), health promoting behavior(r=-,519 p<.001) showing a negative correlation. The factors affecting burnout were organizational support(β=-.113, p=.045), sleep disorder(β=-.129, p<.040), gender(β=-.131, p=.024), health promotion behaviors(β=-.423, p<.001) and the explanatory power was 32.2%. Based on these results, we aim to provide basic data for the development of various programs that can control burnout of nurses.