• Title/Summary/Keyword: miR-21

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Effects of Mixed Medicinal Herbs on Adipogenesis and Apoptosis in 3T3-L1 Adipocytes (배합된 한약재의 추출물이 3T3-L1 지방세포의 지방생성과 세포사멸에 미치는 영향)

  • Hwang, Su-Jung;Shin, In-Soon;Kim, Mi-Ryeo
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.24 no.4
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    • pp.661-667
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    • 2010
  • Astragali Radix (AR) has been used in traditional medicinal herb, which is known to tonify the qi and blood. It has been asserted to be a tonic that can improve the function of the lungs, adrenal glands and the gastrointestinal tract, increase metabolism, promote healing and reduce fatigue. Also, Plantago asiatica (PA) has the anti-inflammatory, antiseptic, antitussive, cardiac, diuretic, expectorant, haemostatic effects, though it is considered to be less powerful than the seed. Recent research is showing that it is promising in lowering cholesterol and controlling diabetes. Obesity is characterized at the cellular level by an increase in the number and size of adipocytes differentiated from fibroblastic preadipocytes in adipose tissue. In the present study, we investigated the effects of 3 to 7 extracts of mixed medicinal herbs (water and ethanol extract of Astragali Radix; ARW and ARE, water and ethanol extract of Plantago asitica; PAW and PAE, and those mixed extracts; ARW+PAE, ARW+PAW and ARE+PAE) on adipogenesis and apoptosis in 3T3-L1 adipocytes by MTT assay, Oil-Red-O staining and TUNEL assay. And then, we determined total contents of phenolic compounds and flavonoid compounds in mixed herbal extracts. These result showed that herbal extracts (ARW, PAE and ARW+PAE) affected on adipogenesis and apoptosis in 3T3-L1 adipocytes among 3 to 7 extracts tested. Especially herbal mixed extracts (ARW+PAE) had the highest inhibition on differentiation of preadipocytes and lipoid accumulation of maturing adipocytes. And it did not affect cell viability of mature adipocytes, but herbal mixed extracts (ARW+PAE) increased apoptosis in mature adipocytes, as proved by highest concentration $200\;{\mu}g/m{\ell}$ using TUNEL assay. In addition, contents of total phenoilc compounds and flavonoids showed the highest level in ARE+PAE and PAE than ARW. These results suggest that this main target for adipocyte functions could be effective in improving the metabolic syndrome including obesity.

The correlation among the oral & facial states and the gummy smile in female college students (일부 여대생의 구강 및 안모상태와 치은노출(Gummy smile)과의 상관성)

  • So, Mi-Hyun
    • Journal of Korean society of Dental Hygiene
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    • v.12 no.2
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    • pp.345-353
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    • 2012
  • Objectives : The author has studied about correlation of gingival exposure upon smiling and oral facial status that reduce facial aesthetic. Methods : The subjects in this study are 91 female vulunteers who were in aged $21.4{\pm}1.89$ in Suwon. Objectives should be normal oral and facial status without the prosthodontic, orthodontic appliance or conqenital missing tooth, and agree to be examined the oral status and impression taking. 1.Measure the length of gingival exposure upon smiling. 2.Measure of the size on central incisor. 3.Measure of Facial. SPSS(SPSS 10.0 for windows, SPSS Inc, Chicago, USA) was utilized for calculating the correlation coefficient between gingival exposure upon smiling and facial status. Regression analysis was calculated in order to predict the R square for gingival exposure upon smiling. Results : 1.Correlation coefficient between the gingival exposure and length of maxillary central incisor was calculated as reversed correlation(r=-.302, p<0.01), and between the gingival exposure and the ratio of the length of central incisor/width of central incisor was revealed as reversed correlation(r=-.250, p<0.05) on smiling. 2.There was correlation between the gingival exposure and the facial height(r=.351, p<0.01), the lower facial height(r=.454, p<0.01) and the upper lip height(r=.274, p<0.01) upon smiling. 3.There was correlation between the gingival exposure and the ratio of the facial height/facial width(r=.358, p<0.05), the ratio of the upper facial height/facial width(r=.214, p<0.05), and the ratio of the lower facial height/facial height(r=.383, p<0.01) upon smiling. 4.The equation of the regression analysis for gingival exposure upon smiling could be estimated as gingival exposure upon smiling=-5.139+.279${\times}$lower facial height-.615${\times}$maxillary central incisal length-.05${\times}$nasolabial angle. Conclusions : Considering these results, it recommended that treatment planning should be designed in consideration of such factors as the length of maxillary central incisor, facial height, upper lip height and lower facial height, in order to promote the easthetic problems of face on smiling.

An Inter-Laboratory Comparison Study on Chlorophyll a Determination in Seawater (해수 중 엽록소 a 측정방법에 대한 실험실 간 비교연구)

  • Moon, Cho-Rong;Kang, Dong-Jin;Park, Mi-Ok;Noh, Jae Hoon;Yoo, In-Jae;Moon, Jeong-Eon;Shin, Kyung-Hoon;Kim, Yun Sook;Choi, Joong-Ki;Suh, Young Sang
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.19 no.1
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    • pp.76-87
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    • 2014
  • Chlorophyll a in seawater which is an indicator of phytoplankton biomass and primary production is determined by High Performance Liquid Chromatography (HPLC), Fluorometry and Spectrophotometry. In this study, various methods for chlorophyll a determination in seawater are compared using in situ seawater samples from Korean seas. Three inter-laboratory comparison campaigns were carried out using chlorophyll a standard samples (R0) and in situ seawater samples, collected from the East China Sea (R1) and the East Sea (R2). 6 laboratories by HPLC methods, 4 laboratories by fluorometry, and 3 laboratories by spectrophotometry participated. Precisions, defined as the coefficient of variation (CV) were within 9% in standard samples, 0.8~20% (average: 6.1%) in R1, 4~21% (average: 13.2%) in R2. Discrepancy in three methods was approximately 20% within the range of the sample homogeneity intended the laboratory precision (R1: 8%, R2: 15%). The discrepancy in laboratories was greater than the discrepancy in methods. The chlorophyll a concentrations can be produced within 20% discrepancy in spite of using different methods. It is recommended to consider this 20% discrepancy when using the chlorophyll a data produced by different laboratories and different methods.

The Effects of Life Stress and Stress Coping among Nursing College Students on Health Promotion Behavior (간호대학생의 생활 스트레스, 스트레스 대처가 건강증진행위에 미치는 영향)

  • Choi, Yooun-Sook;Kim, Mi-Young
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.21 no.7
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    • pp.109-119
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    • 2020
  • The purpose of this study was to provide basic data for improving health promotion behavior by identifying the effects of life stress and coping with stress among nursing college students on health promotion behavior. The data was collected from 352 nursing students in 6 colleges in B and K city, and the data was analyzed with SPSS/Win 23.0. Factors influencing health promotion behavior were school life satisfaction (F=9.18, p<.001), happiness score (F=20.64, p<.001), health status (F=12.40, p<.001), eating breakfast (t=-3.31, p=.001), unbalanced diet (t=-4.20, p<.001), time spent sleeping (F=2.64, p=.049), and exercise (t=5.20, p<.001). In the correlations, health promotion behavior was negatively correlated with life stress (r=-.289, p<.001), but it was positively correlated with coping with stress (r=.539, p<.001). The factors influencing health promotion behavior were stress coping (β=.439, p<.001), happiness score (β=.251, p<.001), exercise (β=.172, p<.001), unbalanced diet (β=.125, p=.002), life stress (β=-.121, p=.005), and eating breakfast (β=.083, p=.046). The above variables explained 46.0% for the health promotion behavior. Thus, in order to improve the health promotion behavior of nursing college students, integrated nursing interventions need to be developed through improving coping with stress, creating a sense of happiness, reducing life stress, and maintaining exercise and good eating habits.

The Contribution of Maternal-Fetal Attachment: Taegyo, Maternal Fatigue and Social Support during Pregnancy (태아애착에의 영향요인: 임부의 피로, 사회적지지, 태교실천)

  • Yu, Mi;Kim, Miok
    • Child Health Nursing Research
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    • v.20 no.4
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    • pp.247-254
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    • 2014
  • Purpose: Maternal-fetal attachment in a pregnant woman is important for her health and for her child's emotional stability. This study was done to identify the contribution of maternal fatigue, social support, and Taegyo to maternal-fetal attachment based on a survey of pregnant women. Methods: The study was a descriptive correlation study. Participants were 211 from two women's health clinic centers. Data were analyzed using t-test, ANOVA, Pearson correlation coefficients and stepwise multiple regression. Results: Mean scores for maternal-fetal attachment, Taegyo, maternal fatigue, and social support were 93.74 (range 25-125), 64.76 (17-85), 24.12 (10-40), and 54.43 (22-132), respectively. There were significant differences in maternal-fetal attachment according to parity, breast feeding experience, and putting prenatal education into practice. Multiple regression analysis showed that the key determinants of maternal-fetal attachment, were Taegyo (${\beta}$=.67), maternal fatigue (${\beta}$=.21), and social support (${\beta}$=.13), and these explained 55% of the total variance of attachment. Conclusion: The results suggest a need to encourage Taegyo in prenatal management program and provide methods for fatigue resolution and promotion of social support.

Factors Influencing Acculturative Stress of Migrant Workers (외국인 근로자의 문화적응 스트레스 영향요인)

  • Kim, Ji-Hyun;Kim, Bo-Mi
    • Journal of Digital Convergence
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    • v.15 no.8
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    • pp.277-287
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    • 2017
  • The purpose of this study was to identify acculturative stress, social support and self esteem in migrant workers. The data of 181 subjects were collected from June 1st to September 27, 2016, and analyzed using the SPSS WIN 23.0 program. Results: There was a significant negative correlation among acculturative stress, social support(r=-.465, p<.001) and self esteem(r=-353, p<.001). Stepwise multiple regression analysis showed self esteem(${\beta}=-.21$, p<.001) and job(${\beta}=-.02$, p<.05) were significant factors affecting acculturative stress(F=27.672, p<.001). The results of the study indicate that the efforts, to improve self esteem, and to increase social support, are required to decrease acculturatives stress among migrant wokers. The results are expected to be utilized in development of supportive program for migrant workers adaptation. In the following study, the analysis of structural equation modeling about additional factors of acculturative stress among migrant workers will be needed.

Light Efficiency of LED Package with TiO2-nanoparticle-dispersed Encapsulant (TiO2 나노입자가 혼합된 봉지재를 적용한 LED 패키지의 광효율 특성 평가)

  • Lee, Tae-Young;Kim, Kyoung-Ho;Kim, Mi-Song;Ko, Eun-Soo;Chio, Jong-Hyun;Moon, Kyoung-Sik;Kim, Mok-Soon;Yoo, Sehoon
    • Journal of the Microelectronics and Packaging Society
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    • v.21 no.3
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    • pp.31-35
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    • 2014
  • $TiO_2$-nanoparticle-dispersed silicone was applied to a LED package and the light efficiency of the LED package was evaluated in this study. The addition of $TiO_2$ nanoparticles in silicone increased refractive index, which improved the light efficiency of the LED package. The $TiO_2$ nanoparticles were fabricated by hydrothermal synthesis and were dispsersed by a vinyl silane coating treatment. After the silane treatment, the $TiO_2$ nanoparticles dispersed with diameters of 10~40 nm but rod-shape $TiO_2$ nanoparticles with lengths of 100 nm were also observed. The refractive index increased with the $TiO_2$ concentration in silicone, while the transmittance decreased with the $TiO_2$ concentration. The light efficient of the LED package with $TiO_2$+silicone encapsulant was higher than that of the LED package with no $TiO_2$ in silicone encapsulant.

Irradiation-induced Volatile Compounds in Irradiated Chicken by P&T Method (P&T법에 의한 닭고기의 방사선 유래 휘발성 조사물질 구명)

  • Kim, Hun;Cho, Woo-Jin;Jung, Yeon-Jung;Lee, Young-Mi;Jeong, Eun-Jeong;Yoo, Young-Jae;Byun, Myung-Woo;Cha, Yong-Jun
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.31 no.6
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    • pp.958-964
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    • 2002
  • To select the irradiation-induced marker components from volatile flavor compounds in irradiated chicken, and complement the extraction problems of liquid continuous extraction (LLCE) method, the volatile compounds of irradiated (0,1,3,5 and 10 kGy) chicken were analyzed by Purge and Trap (P&T) and gas chromatography/mass spectrometry (GC/MS) methods. A total of 119 compounds were detected in irradiated chicken, and these compounds were composed mainly of 7 aldehydes,22 ketones,8 alcohols,30 esters,36 hydrocarbons,8 aromatic compounds and 8 miscellaneous compounds. Among these, only 21 compounds were detected in both LLCE and P&T methods, and the 98 other were detected in omly P&T method. Among volatile compounds detected in irradiated chicken, only 3 compounds such as hexene (r=0.96, p<0.01), propanol (r=0.93, p<0.05) and 1,3-bis(1,1-dimethylethyl) benzene (r=0.96, p<0.05) were newly selected as marker compounds in irradiated chicken by P&T method, which showed significant and high positive correlation coefficient in the change of relative concentration according to the increment of irradiation dosage.

Relations of Postpartum Depression with Socio-Demographic and Clinical Characteristics of Preterm Infants and Mothers (미숙아와 어머니의 인구사회학적, 임상적 특성과 산후우울과의 관련성)

  • Bang, Kyung-Sook;Kang, Hyun-Ju;Kwon, Mi-Kyung
    • Child Health Nursing Research
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    • v.21 no.1
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    • pp.1-10
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    • 2015
  • Purpose: This study was conducted to explore relationships of postpartum depression with socio-demographic and clinical characteristics of preterm infants and mothers. Methods: Participants were the mothers of 80 premature infants admitted to neonatal intensive care units. Postpartum depression was measured using the Edinburg Postnatal Depression Scale (EPDS). Clinical characteristics were examined through the medical records. The physiological state for the infants was evaluated using the revised Neurobiologic Risk Score (NBRS). Data were analyzed using the t-test, ANOVA, and Pearson correlation coefficients. Results: Average for EPDS was $9.75{\pm}5.06$, and 30% of the participants were at high risk for postpartum depression. There were significant differences in postpartum depression according to mother's education level (F=3.493, p=.035), economic state (F=5.828, p=.004), multiple pregnancy (t=2.141, p=.037), chorioamnionitis (t=2.349, p=.021), oligohydramnios (t=-2.226, p=.029), broncho-pulmonary dysplasia (t=2.085, p=.040), germinal matrix hemorrhage (t=2.259, p=.027), and revised NBRS (t=-2.772, p=.007). There was a significant positive correlation between postpartum depression and number of health problem of infants (r=.252, p=.024) and revised NBRS (r=.316, p=.004). Conclusion: As 30% of the mothers with preterm infants were at high risk for postpartum depression, they require attention. When providing interventions, socioeconomic status as well as the physiological state of premature infants should be considered.

A Study on Thermal Effects for Structure of Portable Butane Gas Stoves using Oversized Grill (과대불판 사용 시 이동식부탄연소기 구조에 따른 열영향 연구)

  • Choi, Suel-Ki;Kim, Mi-Sung;Jo, Young-Do;Kim, Young-Gu
    • Journal of the Korean Institute of Gas
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    • v.21 no.1
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    • pp.87-93
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    • 2017
  • This study was conducted to analyze thermal effects for the structure of portable butane gas stoves in accordance with the introduction of safety standard to protect gas accidents that were caused by using oversized grills. The temperatures of oversized grill, cartridge and cover of cartridge coupling part were measured with stoves that heat sink was installed the inside of cover of cartridge coupling part and had different height of tripods. The trend of measured temperatures affected by thermal effects of grill and flame was analyzed. The condition of using portable butane gas stove safely is that the distance between grill and cover of cartridge coupling part should be about 28 mm and over. In contrast, when the distance between grill and cover of cartridge coupling part would be shorter, thermal effects of not only grill but also flame could affect the cartridge directly. In this case, the danger that could cause gas accident would be higher. When heat sink was installed the inside of cover of cartridge coupling part, it was effective on reducing of thermal effects. However, it still had the danger of explosive gas accident of cartridge due to overheating.