• Asian Pacific Journal of Cancer Prevention
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• v.14 no.1
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• pp.139-143
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• 2013

Patients at the same pathological stage of esophageal cancer (EC) that received the same surgical therapy by the same surgeon may have distinct prognoses. The current study aimed to explore the possibility of differentially-expressed microRNAs (miRNAs) underlying this phenomenon. Samples were collected from EC patients at the same tumor node metastasis (TNM) stage but with different prognoses. Paracancerous normal tissues were taken as controls. The specimens were histopathologically analyzed. Differentially-expressed miRNAs were analyzed using real-time quantitative reverse transcription polymerase chain reaction. Compared with patients with poor prognosis, those with good prognosis exhibited 88 two-fold or more than two-fold increased miRNA fragments and 4 half-decreased miRNAs. The most noticeably up-regulated miRNAs included hsa-miR-31, hsa-miR-196b, hsa-miR-652, hsa-miR-125a-5p, hsa-miR-146b, hsa-miR-200c, hsa-miR-23b, hsa-miR-29a, hsa-miR-186, hsa-miR-205, hsa-miR-376a, hsa-miR-410, hsa-miR-532-3p, and hsa-miR-598, whereas the most significantly-downregulated miRNAs were hsa-let-7e, hsa-miR-130b, and hsa-miR-103. EC patients at same TNM stage but with different prognoses show differentially-expressed miRNAs.

• Molecules and Cells
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• v.43 no.11
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• pp.953-963
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• 2020

Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is an infectious disease with multiple severe symptoms, such as fever over 37.5℃, cough, dyspnea, and pneumonia. In our research, microRNAs (miRNAs) binding to the genome sequences of severe acute respiratory syndrome coronavirus (SARS-CoV), Middle East respiratory-related coronavirus (MERS-CoV), and SARS-CoV-2 were identified by bioinformatic tools. Five miRNAs (hsa-miR-15a-5p, hsa-miR-15b-5p, hsa-miR-195-5p, hsa-miR-16-5p, and hsa-miR-196a-1-3p) were found to commonly bind to SARS-CoV, MERS-CoV, and SARS-CoV-2. We also identified miRNAs that bind to receptor proteins, such as ACE2, ADAM17, and TMPRSS2, which are important for understanding the infection mechanism of SARS-CoV-2. The expression patterns of those miRNAs were examined in hamster lung samples infected by SARS-CoV-2. Five miRNAs (hsa-miR-15b-5p, hsa-miR-195-5p, hsa-miR-221-3p, hsa-miR-140-3p, and hsa-miR-422a) showed differential expression patterns in lung tissues before and after infection. Especially, hsa-miR-15b-5p and hsa-miR-195-5p showed a large difference in expression, indicating that they may potentially be diagnostic biomarkers for SARS-CoV-2 infection.

• Asian-Australasian Journal of Animal Sciences
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• v.30 no.7
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• pp.1037-1047
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• 2017

Objective: Despite an increasing number of investigations into the pathophysiology of necrotic enteritis (NE) disease, etiology of NE-associated diseases, and gene expression profiling of NE-affected tissues, the microRNA (miRNA) profiles of NE-affected poultry have been poorly studied. The aim of this study was to induce NE disease in the genetically disparate Fayoumi chicken lines, and to perform non-coding RNA sequencing in the intestinal mucosal layer. Methods: NE disease was induced in the Fayoumi chicken lines (M5.1 and M15.2), and non-coding RNA sequencing was performed in the intestinal mucosal layer of both NE-affected and uninfected chickens to examine the differential expression of miRNAs. Next, quantitative real-time polymerase chain reaction (real-time qPCR) was performed to further examine four miRNAs that showed the highest fold differences. Finally, bioinformatics analyses were performed to examine the four miRNAs target genes involvement in the signaling pathways, and to examine their interaction. Results: According to non-coding RNA sequencing, total 50 upregulated miRNAs and 26 downregulated miRNAs were detected in the NE-induced M5.1 chickens. While 32 upregulated miRNAs and 11 downregulated miRNAs were detected in the NE-induced M15.2 chickens. Results of real-time qPCR analysis on the four miRNAs (gga-miR-9-5p, gga-miR-20b-5p, ggamiR-196-5p, and gga-let-7d) were mostly correlated with the results of RNAseq. Overall, ggamiR-20b-5p was significantly downregulated in the NE-induced M5.1 chickens and this was associated with the upregulation of its top-ranking target gene, mitogen-activated protein kinase, kinase 2. Further bioinformatics analyses revealed that 45 of the gene targets of gga-miR-20b-5p were involved in signal transduction and immune system-related pathways, and 35 of these targets were predicted to interact with each other. Conclusion: Our study is a novel report of miRNA expression in Fayoumi chickens, and could be very useful in understanding the role of differentially expressed miRNAs in a NE disease model.

• Journal of Korean Academy of Fundamentals of Nursing
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• v.11 no.1
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• pp.59-66
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• 2004

Purpose: The purpose of this study was to investigate the relationship between eating disorder, self-esteem and depression in college women. Method: Participants were 282 college women. Data were collected between Nov. 1st and 30th, 2003, and provide basic data on eating disorder levels in college women and basic data for health control programs. The instrument was a questionnaire consisting of 8 items on general characteristics and weight control, 24 on eating disorders, 10 on self-esteem and 10 on depression. Analysis of the data was done using numbers, percentages, means and standard deviations, t-test and Pearson correlation coefficients. SPSS WIN 10.0 was used in data analysis. Result: The greatest difference for eating disorders was between the group with normal body weight and the group with low body weight (t=-6.94, p=.000). There was a high positive correlation between Body Mass Index and eating disorder (r=.383, p<.01), between eating disorder and depression (r=.161, p<.01). There was a high negative correlation between eating disorder and self-esteem (r=-.196, p<.01), and between self-esteem and depression (r=-.537, p<.01). Conclusion: These results indicate that college women need more education and counseling on dietary concerns. Also, systematic efforts to establish a more health conscious social standard for beauty should be taken. Further empirical and experimental studies are required to investigate factors influencing attitudes towards eating held by college women and to determine variables which affect various specific dimensions of these attitudes.

• Microbiology and Biotechnology Letters
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• v.37 no.3
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• pp.189-196
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• 2009

Bacteria were isolated from roots of plants belonging to family Solanaceae and Gramineae, inhabited in Dokdo island. Fifty six endospore-forming bacteria grown on tryptic soy broth (TSB) agar medium and 23 nitrogen-fixing bacteria (NFB) grown on nitrogen free agar medium were isolated, respectively. The isolates were partially identified by analyzing the 16S rDNA and categorized into phylogenetic groups. The 16S rDNA sequences of each identified isolates were compared with sequences of each type strains to analyze phylogenetic relationship by phylogenetic tree. As a result, endospore-forming bacteria and nitrogen-fixing bacteria were classified into 4 and 6 lineage groups, respectively. Among these isolated, 18 were presumed to be novel species candidates based on the similarity (lower than 98%) analysis of the l6S rDNA sequences.

• Physical Therapy Rehabilitation Science
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• v.9 no.4
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• pp.261-268
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• 2020

Objective: This study was conducted to provide basic data on physical function improvement activities of elderly care facilities by identifying the degree of physical improvement activities of elderly caregivers. Design: Descriptive correlational study. Methods: The instrument of this study consisted of 12 questions on the general characteristics of the subject. For the assessment of improvement in physical function activities, the measuring tool used for the elderly consisted of a total of 20 questions. For data analysis, Pearson's correlation coefficient & Spearman's rho and multiple regression were used. Results: The higher the age of the subjects, the lower their educational background (r=-0.273, p<0.05), and the higher the probability of having a religion (r=-0.258, p<0.05), the more stable the employment type (r=-0.333, p<0.05). The higher the level of education, the higher the monthly income (r=0.187, p<0.01), and the shorter the career (r=-0.204, p<0.05). The more stable employment, the more unstable duty (r=-0.245, p<0.05), and the more unstable work, the higher the monthly income (r=-0.206, p<0.05) and the longer the career, the higher the monthly income (r=0.247, p<0.05). The more stable the employment, the more activities to improve physical function were found (r=0.341, p<0.05), and the more unstable the duty, the more activities to improve physical function were found (r=0.321, p<0.05), and the higher monthly income, the less physical function improvement activities (r=-0.196, p<0.05). Conclusions: It is necessary to provide regular services by a dedicated physical therapist for physical function improvement activities in order to improve the quality of life of the elderly in the future.

• BMB Reports
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• v.47 no.4
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• pp.192-196
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• 2014

RNA polymerase II carboxyl-terminal domain (pol II CTD) phosphatases are a newly emerging family of phosphatases that are members of DXDX (T/V). The subfamily includes Small CTD phosphatases, like SCP1, SCP2, SCP3, TIMM50, HSPC129 and UBLCP. Extensive study of SCP1 has elicited the diversified roles of the small C terminal domain phosphatase. The SCP1 plays a vital role in various biological activities, like neuronal gene silencing and preferential Ser5 dephosphorylation, acts as a cardiac hypertrophy inducer with the help of its intronic miRNAs, and has shown a key role in cell cycle regulation. This short review offers an explanation of the mechanism of action of small CTD phosphatases, in different biological activities and metabolic processes.

• Korean Journal of Health Education and Promotion
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• v.19 no.4
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• pp.33-44
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• 2002

This study was conducted to investigate the relationship between an eating disorder, body image, depression and self-esteem amongst a subject group of 282 college women between Jan.,1st and 30th,2002, to provide base data for eating disorder levels of college women and to provide base data for health control. The evaluative instrument was a constructive questionnaire that consisted of 8 items of general characteristics and weight control questions, 24 questions relating to eating disorders, 17 questions pertaining to body image, 10 questions pertaining to depression and 10 questions pertaining to self-esteem. To identify the levels of an eating disorder, body image, depression and self-esteem perceived by subjects, the researcher used means and SDs. To test the significance of a subject's eating disorder, body image, depression and self-esteem according to the BMI, the researcher used the t-test. To test the correlation between the BMI, an eating disorder, body image, depression and self-esteem, the researcher used Pearson correlation coefficient. The results of this study are as follows. The mean score for anorexia nervosa was 31.52 and the mean score for bulimia nervosa was 24.12. Differences between the underweight group and the normal weight group in the level of eating disorders were significant (t=-6.94, p=.000). There were high positive correlations between the BMI and an eating disorder (r=.383, p<.01), between an eating disorder and depression (r=.161, p<.01), between body image and self-esteem (r=.653, p<.01). In addition, there were high negative correlations between an eating disorder and body image (r=-.214, p<01), between an eating disorder and self-esteem (r=-.196, p<.01), between depression and body image (r=-.541, p<.01), and between depression and self-esteem (r=-.537, p<.01). These results indicate that Korean college women need more education and counseling on dietary habits. Also, the systemic efforts reestablish the social standard of the beauty should be taken. Further empirical and experimental studies would be required for investigating for the factors influencing the eating attitude of the college women and discriminating the variables affecting the various specific dimensions of the eating attitude.

• Research in Community and Public Health Nursing
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• v.21 no.3
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• pp.333-340
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• 2010

Purpose: The purpose of this study was to examine the bone mineral density (BMD), body mass index (BMI), stress, and health promotion lifestyle of female college students and to assess relations among them. Methods: A total of 220 female college students were assessed through anthropometric measurements and bone mineral density test using quantitative ultrasound. In addition, the subjects were asked about stress and health promotion lifestyle with a self-rating questionnaire. Collected data were processed with the SPSS/WIN 12.0 program. Results: 1) Of the students, 67.8% had weight control experience. The percentage of the osteoporosis, osteopenia and normal groups were 2.8%, 53.5% and 43.8%, respectively, and the percentage of the underweight, normal and overweight groups according to BMI were 27.9%, 57.2% and 14.9%, respectively. 2) The levels of stress and health promotion lifestyle were 2.9 and 2.3, respectively. 3) There was a positive correlation between BMD and BMI (r=.196, p<.01). There was a negative correlation between stress and health promotion lifestyle (r=-.35, p<.01). 4) Weight control experience made negative effects on BMD and BMI. Conclusion: The findings of this study suggest the necessity of new health promotion programs to increase bone density of female college students. Stress management programs are also needed.

• Journal of the Korea Convergence Society
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• v.8 no.7
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• pp.113-122
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• 2017

The purpose of this study is to verify trust in supervisor, customer badness behavior have turnover intention influence on job embeddedness. Self-report questionnaire was completed by 188 clinical nurse May 1st to May 30th, 2016. and date analyzed using the IBM SPSS/WIN 21.0 program. As a result, The mean score of trust in supervisor is 3.20 points, customer badness behavior is 2.66 points, job embeddedness is 3.05 points, turnover intention is 3.39 points(out of 5). There were significant positive correlations between job embeddedness and trust in supervisor(r=.57, p<.001) and turnover intention(r=.19, p=.011) and negative correlations between Sacrifice sub-cale of job embeddedness and customer badness behavior(r=-.15, p=.037). The convergence factors with the effect on job embeddedness, trust in supervisor(${\beta}=.471$, p<.001), job satisfaction(${\beta}=.226$, p<.001), turnover intention(${\beta}=-.196$, p<.001), nursing satisfaction(${\beta}=-.169$, p=.005) explained 48.9% of the job embeddedness. therefore, nursing managers have to strategy increasing nurse trust in supervisor and job satisfaction and nursing satisfaction for job embeddedness and plan to lower the turnover intention at the organization level.