• Title/Summary/Keyword: miR-155

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Safety assessments of recombinant DTaP vaccines developed in South Korea

  • Gi-Sub Choi;Kyu-Ri Kang;Seung-Bum Kim;Joon-Hwan Ji;Gyu-Won Cho;Hyun-Mi Kang;Jin-Han Kang
    • Clinical and Experimental Vaccine Research
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    • v.13 no.2
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    • pp.155-165
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    • 2024
  • Purpose: Pertussis bacteria have many pathogenic and virulent antigens and severe adverse reactions have occurred when using inactivated whole-cell pertussis vaccines. Therefore, inactivated acellular pertussis (aP) vaccines and genetically detoxified recombinant pertussis (rP) vaccines are being developed. The aim of this study was to assess the safety profile of a novel rP vaccine under development in comparison to commercial diphtheria-tetanus-acellular pertussis (DTaP) vaccines. Materials and Methods: The two positive control DTaP vaccines (two- and tri-components aP vaccines) and two experimental recombinant DTaP (rDTaP) vaccine (two- and tri-components aP vaccines adsorbed to either aluminum hydroxide or purified oat beta-glucan) were used. Temperature histamine sensitization test (HIST), indirect Chinese hamster ovary (CHO) cell cluster assay, mouse-weight-gain (MWG) test, leukocytosis promoting (LP) test, and intramuscular inflammatory cytokine assay of the injection site performed for safety assessments. Results: HIST results showed absence of residual pertussis toxin (PTx) in both control and experimental DTaP vaccine groups, whereas in groups immunized with tri-components vaccines, the experimental tri-components rDTaP absorbed to alum showed an ultra-small amount of 0.0066 IU/mL. CHO cell clustering was observed from 4 IU/mL in all groups. LP tests showed that neutrophils and lymphocytes were in the normal range in all groups immunized with the two components vaccine. However, in the tri-components control DTaP vaccine group, as well as two- and tri-components rDTaP with beta-glucan group, a higher monocyte count was observed 3 days after vaccination, although less than 2 times the normal range. In the MWG test, both groups showed changes less than 20% in body temperature and body weight before the after the final immunizations. Inflammatory cytokines within the muscle at the injection site on day 3 after intramuscular injection revealed no significant response in all groups. Conclusion: There were no findings associated with residual PTx, and no significant differences in both local and systemic adverse reactions in the novel rDTaP vaccine compared to existing available DTaP vaccines. The results suggest that the novel rDTaP vaccine is safe.

DNA marker traceability in Korean Cattle

  • Kwon, Jae-Chul;Choi, Yu-Mi;Lee, Jea-Young
    • 한국데이터정보과학회:학술대회논문집
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    • 2006.11a
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    • pp.155-159
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    • 2006
  • Samples of 33 Hanwoo individuals from Korean elite sire families were used and five microsatellite markers were selected finally, which were located on chromosomes different chromosomes with the end sequencing of 100 HW-YUBAC that were recorded in the NCBI by Yeungnam University. Ten major microsatellite markers were selected from alleles amplified, their frequencies, H(Heterozygosity) and PIC(Polymorphism information content) with Hardy-Weinberg equilibrium. Next, in order to evaluate the Power of the markers selected on the individual animal identification, the match probability(MP) and the relatedness coefficient(R) were computed.

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Impact of Self-Esteem and Job Stress on Job Satisfaction of the Emergency Medical Technicians (응급구조사의 자아존중감, 직무스트레스가 직무만족도에 미치는 영향)

  • Kim, Mi-Sook;Kim, Nam-Hee
    • The Korean Journal of Health Service Management
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    • v.5 no.2
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    • pp.147-155
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    • 2011
  • The purpose of this study was to investigate self-esteem and job stress which might influence job satisfaction of the emergency medical technicians. The partcipants were 135 emergency medical technicians in hospital. Data were collected from August 15 to September 10, 2009. The collected data was analyzed with the SPSS win 15.0 version. The average self-esteem score was 3.47, job stress 3.16, job satisfaction 3.14. The score for job satisfaction. showed significant difference motivation of desire(F=3.169, p=.016) and region(F=3.414, p=.011). Self-esteem had significant positive correlation to job satisfaction(r=.296, p=.000). Job stress had significant negative correlation to job satisfaction(r=-.327, p=.000). Self-esteem and job stress were significant predictor and accounted for 16% of the variance in job satisfaction of the emergency medical technicians. Future job satisfaction management program for the emergency medical technicians should be considered their self-esteem and job stress.

Application of Near Infrared Spectroscopy for Nondestructive Evaluation of Color Degree of Apple Fruit (사과 착색도의 비파괴측정을 위한 근적외분광분석법의 응용)

  • Sohn, Mi-Ryeong;Cho, Rae-Kwang
    • Food Science and Preservation
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    • v.7 no.2
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    • pp.155-159
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    • 2000
  • Apple fruit grading is largely dependant on skin color degree. This work reports about the possibility of nondestructive assessment of apple fruit color using infrared(NIR) reflectance spectroscopy. NIR spectra of apple fruit were collected in wavelength range of 1100~2500nm using an InfraAlyzer 500C(Bran+Luebbe). Calibration as calculated by the standard analysis procedures MLR(multiple linear regression) and stepwise, was performed by allowing the IDAS software to select the best regression equations using raw spectra of sample. Color degree of apple skin was expressed as 2 factors, anthocyanin content by purification and a-value by colorimeter. A total of 90 fruits was used for the calibration set(54) and prediction set(36). For determining a-value, the calibration model composed 6 wavelengths(2076, 2120, 2276, 2488, 2072 and 1492nm) provided the highest accuracy : correlation coefficient is 0.913 and standard error of prediction is 4.94. But, the accuracy of prediction result for anthocyanin content determining was rather low(R of 0.761).

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The Photometric Analysis Of The W Subtype Contact Binary EK Comae Berenices

  • Song, Mi-Hwa;Kim, Chun-Hwey;Lee, Jae Woo
    • The Bulletin of The Korean Astronomical Society
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    • v.42 no.2
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    • pp.64.1-64.1
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    • 2017
  • 접촉쌍성 EK Com은 0.2667일의 짧은 주기를 가진 만기형 식쌍성으로 광도곡선의 변화로 인해 연구자마다 이계의 하위 유형을 다르게 언급하여 하위유형의 혼동이 있는 천체이다. 우리는 소백산천문대에서 2009년 5월 VR 필터로, 2013년 2 ~ 5월 사이 BVRI 필터로, 2016년 1 ~ 4월 사이 R 필터로 CCD 측광 관측하여 3개의 새로운 광도곡선을 획득하였다. 우리의 관측 자료와 SuperWASP의 공개된 자료로부터 40개의 새로운 극심시각을 산출하였다. 이 극심시각을 포함하여 155개의 극심시각을 여러 문헌에서 수집하여 주기 연구를 수행한 결과, EK Com의 공전주기는 영년 주기 증가와 더불어 8.2년의 주기적인 변화가 겹쳐 변화함을 발견하였다. 이러한 주기 변화 원인에 대하여 살펴본 결과, 영년주기변화는 질량이 작은 별에서 큰 별로의 질량이동에 의하여 일어나고, 주기적인 변화는 질량이 큰 주성의 자기 활동에 의해 발생할 수 있음을 보였다. Wilson-Devinney code를 이용한 광도곡선 분석을 통하여 EK Com은 개기식이 부식에 있는 하위 유형 A라기 보다 주식에 있는 W형에 속하며, 모든 광도곡선에서 1개의 Hot spot과 1개의 Cool spot을 가진 모형이 가장 관측치를 잘 설명한다. W UMa형 별들의 HR도, 온도비, 질량의 그래프에서 EK Com은 W형 Group들이 있는 위치에 존재한다. 이는 광도곡선에서 유추한 EK Com의 하위 유형과도 일치한다.

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UNCERTAINTIES IN AMV ESTIMATION

  • Sohn, Eun-Ha;Cho, Hee-Je;Ou, Mi-Lim;Kim, Yoon-Jae
    • Proceedings of the KSRS Conference
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    • 2007.10a
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    • pp.153-155
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    • 2007
  • Korea Meteorological Administration (KMA) has operationally produced Atmospheric Motion Vector (AMV) from the consecutive MTSAT-1R satellite image dataset. Comparing with radiosonde data, our current AMV scheme shows more than 10 m/s RMSE. Therefore we need to improve continuously its accuracy. Many AMV producers have stated that the bad performance of the Height Assignment (HA) algorithm is the main reason of degrading the accuracy of AMV. The uncertainties in AMV HA can occur in the algorithm itself, used NWP profiles, and the performance of Radiative Transfer Model (RTM) etc. This study introduces currently operated AMV HA schemes and the impacts of NWP profile data and RTM that these schemes use were investigated. Finally we analyzed the relationship between vectors by vector tracking and heights assigned to each vector by using collocated wind profile dataset with radiosonde data. This study is a preliminary work to improve the accuracy of AMV by removing or decreasing the uncertainties in AMV estimation.

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Relationship between Critical Thinking Disposition and Clinical Decision-Making Abilities in Home Health Advanced Practice Nurses (가정전문간호사의 비판적 사고성향과 임상의사결정능력과의 관계)

  • Choi, Seong Mee;Lee, Mi Kyoung
    • Journal of Home Health Care Nursing
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    • v.21 no.2
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    • pp.147-155
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    • 2014
  • Purpose: Clinical decision-making carried out by nurses is a complex process that influences the quality of care provided and various patient outcomes. This study examined the relationship between critical thinking disposition and clinical decision-making abilities in home health advanced practice nurses. Method: The study had a non-experimental correlational design. Data were collected from 100 home health advanced practice nurses in 20 hospitals. Results: The mean critical thinking disposition score was $3.69{\pm}.39$ out of 5 and the mean score for clinical decision-making abilities was $3.48{\pm}.22$ out of 5. In this correlation analysis, a significant positive correlation (r=.58, p<.001) was found between critical thinking disposition and clinical decision-making abilities of home health advanced practice nurses. Conclusion: In order to improve the clinical decision-making ability of home health advanced practice nurses, we need to improve their critical thinking disposition. In order to make this change, appropriate training program are needed to increase the critical thinking disposition and clinical decision-making abilities of home health advanced practice nurses.

Assessment of Daily steps, Activity coefficient, Body composition, Resting Energy Expenditure and Daily Energy Expenditure in Female University Students (여대생의 1일 보행수, 활동계수, 신체조성, 휴식대사량 및 에너지 소비량의 평가)

  • Choe, Hyeon-Jeong;Song, Ju-Mi;Kim, Eun-Gyeong
    • Journal of the Korean Dietetic Association
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    • v.11 no.2
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    • pp.159-169
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    • 2005
  • The purpose of this study was to assess the energy expenditure and investigate the relationship between related variables in 70 female university students. Resting energy expenditure estimated by Harris-Benedict formula, WHO/NAO/FAO formula and various formulas based on body weight and body surface area were 1366.9$\pm$74.4kcal/day, 1287.8$\pm$106.6kcal/day, 1171.4$\pm$155.8kcal/day and 1342.0$\pm$97.4kcal/day. Measured resting energy expenditure by indirect calorimetry(Model : Metavine and TrueOne2400) were 1582.0$\pm$150.1kcal/day and 1268.2$\pm$152.9kcal/day, respectively. Average step number per day was 11981.2$\pm$3014.4 steps and average step number per hour was 746.1$\pm$198.0 steps/hr. Daily energy expenditure by using Harris-Benedict formula, body weight formula, body surface area formula, WHO/NAO.FAO formula and 15-min check list formula were 2374.7$\pm$249.6kcal, 2033.5$\pm$313.2kcal, 2331.2$\pm$266.0kcal, 2240.8$\pm$185.5kcal and 2195.5$\pm$398.3kcal. Meanwhile energy intake of subjects was 1714.9$\pm$551.2 kcal. Daily energy expenditure has positive correlation(r=0.262) with daily step number. And there was significant positive correlations(r=0.35-0.68) between various daily energy expenditures and muscle mass. These results suggested that increase of daily step number by using pedometer is good method to increase daily energy expenditure. In particular, increase in step number can reinforce the amounts of muscle.

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A Study of the Body Mass Index and Body Image among Female College Students (여자대학생의 비만지수와 신체상에 관한 연구)

  • Chung, Eun-Soon
    • Women's Health Nursing
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    • v.7 no.4
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    • pp.622-630
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    • 2001
  • This study aims at examining relationships between body mass index and perceived body shape and image. Using a purposive sampling method, 404 female college students were recruited from three universities located in Busan from April 2000 to June 2000. To classify the levels of obesity, Broca's method was used. Secord & Jourard's Body Cathexis Scale(1953) modified by Kim mi ok(1997) was also used to measure body image. The collected data were analyzed with the percentages, Mean, Standard deviation, T-test, and Pearson correlation Coefficient. The results of this study were as follows. 1. 56.4 % of subjects were under weight, and 42.1% were normal for BMI. 2. 55.9% of subjects were very satisfied with their body shape and 15.1% were satisfied. 3. The mean values of perceived body shape according to the BMI were 3.0 for the overweight group, 2.65 for the normal weight group, and 2.21 for the low weight group, the mean differences among three groups were significantly satisfied (F= 21.42, P= .000). 4. The mean scores of body image according to the BMI was 160.21 for low weight group, 155.57 for the normal weight group, and 140.0 for overweight group. The mean differences among the three groups were not significantly satisfied. 5. The mean scores of body image according to perceived body shape were 17819 for the satisfaction group, 161.08 for the moderate group and 150.82 for dissatisfied group. The mean differences among the three groups were significantly satisfied (F= 35.72, P=. 000). 6. There were high positive correlations between BMI and perceived body shape(r= .317) and between perceived body shape and body image (r=- .415).

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The Determinants of Health Promoting Behavior in Students on Dept of Dental Hygiene (치위생과 학생의 건강증진행위 결정요인에 관한 연구)

  • Kim, Eun-Mi;Lee, Hyang-Nim
    • Journal of dental hygiene science
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    • v.4 no.3
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    • pp.141-148
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    • 2004
  • This study was examed in order to determine influential factors of health promoting behavior on Dental Hygiene students the health promoting behavior. So examed students' health promoting behavior, self-efficacy, perceived benefit, perceived barrier, a health locus of control, self-esteem. A the result of this study were as follows: (1) Performance mean score in health promoting behavior was 2.60, self achievement score was 2.89, health responsibility score was 2.12, exercise score was 1.89, nutrition score was 2.45, interpersonal support score was 2.97, stress management score was 2.63. Performance mean score in self-efficacy was 2.56, perceived benefit was 3.45, perceived barrier was 2.32, a health locus of control score was 3.04, self-esteem score was 2.81. (2) Performance in health promoting behavior was significant differences in year, religion, economical level, experience of disease on family, perceived health status(p<0.05), perceived oral health status(p<0.001). Performance in self achievement was significant differences in year, economical level, perceived health status(p<0.05), religion, perceived oral health status(p<0.01). Performance in health responsibility was significant differences in year, religion, economical level, BMI(p<0.05) and experience of disease on myself, perceived oral health status(p<0.001). Performance in excercise was significant differences in mother's educational level, experience of disease on family, perceived oral health status(p<0.05) and nutrient was economical level, perceived oral health status(p<0.01), perceived health status(p<0.05). Performance in interpersonal relations was only significant differences perceived oral health status(p<0.05) and in stress management was year, perceived oral health status(p<0.05). (3) Performance in self-efficacy was significant differences in economical level, health status(P<0.05) and perceived health status, perceived oral health status(p<0.01). Performance in perceived benefit was significant differences in religion(p<0.05). Performance in perceived barrier was significant differences economical level, perceived oral health status(p<0.05), experience of disease on myself(p<0.01). Performance in a health locus of control was significant differences year(p<0.05), performance in a perceived oral health status(p<0.01). (4) Performance in health promoting behavior was significantly correlated with self-efficacy(r=0.376), perceived benefit(r=0.188), perceived barrier(r=-0.155), a health locus of control (r=0.064), self-esteem(r=0.318). (5) Self-efficacy was the highest factor predicting health promoting behavior.

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