• Animal Bioscience
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• v.37 no.4
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• pp.609-621
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• 2024

Objective: Hair follicle stem cells (HFSCs) differentiation is a critical physiological progress in skin hair follicle (HF) formation. Goat HFSCs differentiation is one of the essential processes of superior-quality brush hair (SQBH) synthesis. However, knowledge regarding the functions and roles of miR-133a-3p and miR-145-5p in differentiated goat HFSCs is limited. Methods: To examine the significance of chi-miR-133a-3p and chi-miR-145-5p in differentiated HFSCs, overexpression and knockdown experiments of miR-133a-3p and miR-145-5p (Mimics and Inhibitors) separately or combined were performed. NANOG, SOX9, and stem cell differentiated markers (β-catenin, C-myc, Keratin 6 [KRT6]) expression levels were detected and analyzed by using real-time quantitative polymerase chain reaction, western blotting, and immunofluorescence assays in differentiated goat HFSCs. Results: miR-133a-3p and miR-145-5p inhibit NANOG (a gene recognized in keeping and maintaining the totipotency of embryonic stem cells) expression and promote SOX9 (an important stem cell transcription factor) expression in differentiated stem cells. Functional studies showed that miR-133a-3p and miR-145-5p individually or together overexpression can facilitate goat HFSCs differentiation, whereas suppressing miR-133a-3p and miR-145-5p or both inhibiting can inhibit goat HFSCs differentiation. Conclusion: These findings could more completely explain the modulatory function of miR-133a-3p and miR-145-5p in goat HFSCs growth, which also provide more understandings for further investigating goat hair follicle development.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.8
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• pp.3767-3772
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• 2014

Introduction: MicroRNAs have emerged as post-transcriptional regulators that are critically involved in tumorigenesis. This study was designed to explore the effect of miRNA 133b on the proliferation and expression of TBPL1 in colon cancer cells. Methods: Human colon cancer SW-620 cells and human colon adenocarcinoma HT-29 cells were cultured. MiRNA 133b mimcs, miRNA 133b inhibitors, siRNA for TBPL1 and scrambled control were synthesized and transfected into cells. MiR-133b levels in cells and CRC tumor tissue was measured by real-time PCR. TBPL1 mRNA was detected by RT-PCR. Cell proliferation was studied with MTT assay. Western blotting was applied to detect TBPL1 protein levels. Luciferase assays were conducted using a pGL3-promoter vector cloned with full length of 3'UTR of human TBPL1 or 3'UTR with mutant sequence of miR-133b target site in order to confirm if the putative binding site is responsible for the negative regulation of TBPL1 by miR-133b. Results: Real time PCR results showed that miRNA 133b was lower in CRC tissue than that in adjacent tissue. After miR-133b transfection, its level was elevated till 48h, accompanied by lower proliferation in both SW-620 and HT-29 cells. According to that listed in http://www.targetscan.org, the 3'-UTR of TBPL1 mRNA (NM_004865) contains one putative binding site of miR-133b. This site was confirmed to be responsible for the negative regulation by miR-133b with luciferase assay. Further, Western blotting and immunohistochemistry both indicated a higher TBPL1 protein expression level in CRC tissue. Finally, a siRNA for TBPL1 transfection obviously slowed down the cell proliferation in both SW-620 and HT-29 cells. Conclusion: MiR-133b might act as a tumor suppressor and negatively regulate TBPL1 in CRC.

• Animal Bioscience
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• v.36 no.1
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• pp.144-155
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• 2023

Objective: Adipocyte differentiation is regulated by a variety of functional genes and noncoding RNAs. However, the role of miRNAs in lipid deposition of goat white adipose tissue is still unclear. Therefore, this study revealed the miRNA expression profile in goat subcutaneous adipocytes by sRNA-seq. Methods: The miRNA expressed in goat subcutaneous preadipocytes and the mature adipocytes were sequenced by sRNA-seq. The differentially expressed miRNAs (DEm) were screened and gene ontology (GO) and Kyoto encyclopedia for genes and genomes (KEGG) analyses were performed. Gain-of-function and loss-of-function combined with oil red O staining, Bodipy staining, and quantitative reverse-transcription polymerase chain reaction (qPCR) were utilized to determine the effect of miR-133a-3p on adipocyte differentiation. Results: A total of 218 DEm were screened out. The target genes of these DEm were significantly enriched in GO items such as biological regulation and in KEGG terms such as FAK signaling pathway and MAPK signaling pathway. qPCR verified that the expression trend of miRNA was consistent with miRNA-seq. The gain-of-function or loss-of-function of miR-133a-3p showed that it promoted or inhibited the accumulation of lipid droplets, and CCAAT enhancer binding protein α (C/EBPα) and C/EBPβ were extremely significantly up-regulated or down-regulated respectively (p<0.01), the loss-of-function also led to a significant down-regulation of peroxisome proliferator activated receptor gamma (PPARγ) (p<0.01). Conclusion: This study successfully identified miRNAs expression patterns in goat subcutaneous adipocytes, and functional identification indicates that miR-133a-3p is a positive regulator of the differentiation process of goat subcutaneous adipocytes. Our results lay the foundation for the molecular mechanism of lipid deposition in meat-source goats from the perspective of miRNA.

• Journal of Animal Science and Technology
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• v.63 no.4
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• pp.854-863
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• 2021

Weaning induces physiological changes in intestinal development that affect pigs' growth performance and susceptibility to disease. As a posttranscriptional regulator, microRNAs (miRNAs) regulate cellular homeostasis during intestinal development. We performed small RNA expression profiling in the small intestine of piglets before weaning (BW), 1 week after weaning (1W), and 2 weeks after weaning (2W) to identify weaning-associated differentially expressed miRNAs. We identified 38 differentially expressed miRNAs with varying expression levels among BW, 1W, and 2W. Then, we classified expression patterns of the identified miRNAs into four types. ssc-miR-196a and ssc-miR-451 represent pattern 1, which had an increased expression at 1W and a decreased expression at 2W. ssc-miR-499-5p represents pattern 2, which had an increased expression at 1W and a stable expression at 2W. ssc-miR-7135-3p and ssc-miR-144 represent pattern 3, which had a stable expression at 1W and a decreased expression at 2W. Eleven miRNAs (ssc-miR-542-3p, ssc-miR-214, ssc-miR-758, ssc-miR-4331, ssc-miR-105-1, ssc-miR-1285, ssc-miR-10a-5p, ssc-miR-4332, ssc-miR-503, ssc-miR-6782-3p, and ssc-miR-424-5p) represent pattern 4, which had a decreased expression at 1W and a stable expression at 2W. Moreover, we identified 133 candidate targets for miR-196a using a target prediction database. Gene ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses revealed that the target genes were associated with 19 biological processes, 4 cellular components, 8 molecular functions, and 7 KEGG pathways, including anterior/posterior pattern specification as well as the cancer, PI3K-Akt, MAPK, GnRH, and neurotrophin signaling pathways. These findings suggest that miRNAs regulate the development of the small intestine during the weaning process in piglets by anterior/posterior pattern specification as well as the cancer, PI3K-Akt, MAPK, GnRH, and neurotrophin signaling pathways.

• Molecules and Cells
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• v.45 no.3
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• pp.122-133
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• 2022

The aim of this study was to investigating whether lncRNA H19 promotes myocardial fibrosis by suppressing the miR-29a-3p/miR-29b-3p-VEGFA/TGF-β axis. Patients with atrial fibrillation (AF) and healthy volunteers were included in the study, and their biochemical parameters were collected. In addition, pcDNA3.1-H19, si-H19, and miR-29a/b-3p mimic/inhibitor were transfected into cardiac fibroblasts (CFs), and proliferation of CFs was detected by MTT assay. Expression of H19 and miR-29a/b-3p were detected using real-time quantitative polymerase chain reaction, and expression of α-smooth muscle actin (α-SMA), collagen I, collagen II, matrix metalloproteinase-2 (MMP-2), and elastin were measured by western blot analysis. The dual luciferase reporter gene assay was carried out to detect the sponging relationship between H19 and miR-29a/b-3p in CFs. Compared with healthy volunteers, the level of plasma H19 was significantly elevated in patients with AF, while miR-29a-3p and miR-29b-3p were markedly depressed (P < 0.05). Serum expression of lncRNA H19 was negatively correlated with the expression of miR-29a-3p and miR-29b-3p among patients with AF (r_s = -0.337, r_s = -0.236). Moreover, up-regulation of H19 expression and down-regulation of miR-29a/b-3p expression facilitated proliferation and synthesis of extracellular matrix (ECM)-related proteins. SB431542 and si-VEGFA are able to reverse the promotion of miR-29a/b-3p on proliferation of CFs and ECM-related protein synthesis. The findings of the present study suggest that H19 promoted CF proliferation and collagen synthesis by suppressing the miR-29a-3p/miR-29b-3p-VEGFA/TGF-β axis, and provide support for a potential new direction for the treatment of AF.

• Research in Community and Public Health Nursing
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• v.17 no.1
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• pp.125-133
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• 2006

Purpose: This study was performed to identify the relationship between PMS and sex role. Method: Data were collected from 417 female college students in Seoul and Gyeonggi-do area during the period from the 6th of September to the 15th of October in 2004. Collected data were analyzed using SPSS WIN 11.0. Result: The results of this study were as follows: 1) Perceived subjective symptoms were clustered endogenous depressive feature ($2.76{\pm}.97$), anxiety ($2.76{\pm}1.09$), and impulsivity ($2.72{\pm}1.02$). 2) PMS was significantly different according to the general characteristics of menarche age (F=5.910, p=.003), pain (F=7.886. p=.000) and family history (F=5.366, p=.005), masculinity was significantly different according to menarche age (F=3.174, p=.043), and femininity was significantly different according to menarche age (F=3.742, p=.025) and pain (F=3.256. p=.040). 3) Femininity and PMS were in a positive correlation with each other (r=.623. p=.000). Conclusion: This results showed that there is a significant correlation between PMS and sex role. For future research, it is recommended to identify major factors affecting PMS and the relationships between them and various subjects.

• The Korean Journal of Emergency Medical Services
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• v.18 no.3
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• pp.53-62
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• 2014

Purpose: This study aimed to investigate duty-related incidental stress, coping method, and stress factors in 133 new firefighters. Methods: A self-reported questionnaire was administrated to 133 new firefighters between April and May 2010. It consisted of 33 and 62 items concerning duty-related incident stress and coping methods, respectively. Data were analyzed by using the t-test, analysis of variance, Pearson correlation coefficient analysis, and multiple regression analysis. Results: New firefighters experienced the most stress when inappropriately dispatched. Duty-related incidental stress correlated with active (r = .420, p < .001) and passive coping (r = .450, p < .001). Also active coping statistically correlated with passive coping (r = .890, p < .001). Influencing factors of duty-related incidental stress were passive coping (t = 2.12, p < .05), experience of having a co-worker in a dangerous situation (t = 3.30, p < .001), having less than 6 months of work experience (t = 2.30, p < .05), and experience of having oneself in a dangerous situation (t = 2.05, p < .05). Conclusion: New firefighters need to be provided with training on active coping to prevent posttraumatic stress disorders and, social support immediately after an inappropriate and stressful dispatch.

• The Journal of the Convergence on Culture Technology
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• v.10 no.5
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• pp.289-297
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• 2024

This study was attempted to confirm the effects of nursing students' artificial intelligence literacy and grit on their creative convergence capabilities. This study targeted 133 students attending two nursing colleges located in G Province. Data analysis was analyzed using SPSS 21 program with descriptive statistics, t-test, ANOVA, Pearson correlation coefficient, and multiple regression analysis. The average creative convergence competency of the subjects was 3.53 points, and there was a significant difference in creative convergence competency according to general characteristics in grades (F=8.65, p<.005) and major satisfaction (F=3.95, p=.021). there was. The subject's creative convergence capability had a statistically significant positive correlation with artificial intelligence literacy (r=.599, p<.001) and grit (r=473, p<.001). The influencing factors on the subjects' creative convergence capabilities were artificial intelligence literacy (β=0.350, p<.001) and grit (β=0.192, p<.001), and the explanatory power of these variables was 45.6%. Based on the above research results, it is necessary to develop and apply curriculum and extracurricular programs that can improve artificial intelligence literacy and grit in order to improve the creative convergence capabilities of nursing students.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.9
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• pp.166-173
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• 2018

This study was conducted to investigate the effects of workplace bullying and social support on turnover intention of nurses. The data included 133 nurses who worked in general hospitals in Chungchungbukdo. Data were collected from 1 November, 2016 to 25 December using self-report, structured questionnaires. Collected data were analyzed by ANOVA, t-test, Pearson correlation coefficient and multiple regression using SPSS 20.0. The results showed that the effects of workplace bullying and social support on turnover intention averaged 1.46 (out of 4), 3.55 (out of 5) and 3.30 (out of 5). Additionally, there was a significant positive correlation between workplace bullying and turnover intention (r=0.218, p=0.012) and negative correlations between social support and turnover intention (r=-0.363, p<0.001). Multiple regression analysis revealed that the predictors of turnover intension were education (${\beta}=-0.268$, p<0.009), total clinical experience (${\beta}=0.706$, p<0.001), and workplace bullying (${\beta}=0.183$, p<0.036). Based on these results, it is necessary to recognize the importance of workplace bullying and develop and adapt programs to reduce workplace bullying in order to decrease turnover intention among nurses.

• Journal of Korean society of Dental Hygiene
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• v.17 no.1
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• pp.145-153
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• 2017

Objectives: This paper aimed to contribute to better oral disease prevention and practice of health behavior for immigrant women in multi-cultural families, to define missing and filled permanent teeth index of immigrant women, data from the 6th Korea National Health and Nutrition Examination Survey from the Korea Centers for Disease Control and Prevention was used. Methods: For the immigrant women to be subjects, they needed to be born overseas, had acquired Korean citizenship as a married immigrant women, and the estimate of the number of subjects was 133,093 women. For analyzing data, SPSS 21statistical program was used. We used covariance analysis (ANCOVA) andgeneral linear models for finding the relation with the missing and filled permanent teeth index. The significance level was 0.05. Results: DMFT-index of immigrant women was 7.33 points. $R^2$ was 0.416; and increased with age, and $R^2$ was 0.126 points higher (p<0.01). In household income, 'lower' was 5.933 points lower than 'upper' (p<0.05), and in toothbrushing after lunch, 'yes' was 3.598 points lower than 'no' (p<0.01). In preventive treatment, 'yes' was 4.301 points lower than 'no' (p<0.05). Conclusions: The result of this paper is as follows: for maintaining oral health of immigrant women, we think that the government needs to develop an oral health policy and a customized education system suited to immigrant women for preventive management of dental disease in immigrant women. In addition, basic data will be provided for public dental health programs based on the result of the study.