• Title/Summary/Keyword: methylene blue color test

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Application of methylene blue color test for the detection of inherited susceptibility to hemolysis of Korean native cattle (한우(韓牛)의 선천성용혈감수성검사(先天性溶血感受性檢査)에 대한 methylene blue 청색소실시험법(靑色消失試驗法)의 적용(適用))

  • Cho, Jong-hoo
    • Korean Journal of Veterinary Research
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    • v.28 no.2
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    • pp.327-329
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    • 1988
  • Blood samples were obtained from Korean native cattle and dairy cattle of Holstein species in the slaughter house and methylene blue color tests were performed for the detection of the inherited susceptibitity to hemolysis. Glucose-6-phosphate dehydrogenase activities expressed as the optical density obtained by methylene blue color test were the highest as 0.54 in male Korean cattle, 0.62 in female Korean cattle and 0.72 in dairy cattle of Holstein species. Percent hemolysis, packed cell volume and plasma protein contents were measured and compaired with relation to the results of methylene blue color test and no correlation were observed in each.

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Self-Cleaning of Mortar Mixed with Photocatalyst by Using Methylene Blue Solution (메틸렌블루 용액을 이용한 광촉매 혼입 모르타르의 방오성능 평가)

  • Yang, In-Hwan;Park, Ji-Hun;Park, Hee-Woong;Jung, Hoe-Won
    • Journal of the Korean Recycled Construction Resources Institute
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    • v.8 no.3
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    • pp.356-364
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    • 2020
  • An experimental study to investigate the effect of self-cleaning of mortar mixed with photocatalyst was performed out in this study. Test parameters included the photocatalyst content and surface roughness of the specimens. The experimental mortar specimens were manufactured by mixing a photocatalyst by cement weight of 2.5%, 5.0%, 7.5% and 10.0%. In addition, the surface roughness was categorized into three cases. They included flat surface condition, little surface roughness(medium roughness), and high surface roughness. After mortar specimens were cured for 28 days, they were illuminated by an ultraviolet lamp for 24 hours and immersed in a methylene blue conditioning solution for 12 hours. Thereafter, an ultraviolet(UV) lamp was illuminated on the specimens for 48 hours in an experimental chamber and then the color change of methylene blue solution was measured by using a spectrophotometer over illuminating time of UV lamp. The color change of methylene blue tended to increase as photocatalyst contents increased. Test results meant that photocatalyst was effective for self-cleaning in mortar. However, the color change of the methylene blue solution did not show a noticeable tendency at different surface roughness conditions. It might be due to the uneven photocatalyst distribution on the surface of mortar specimens.

Effect of Organic Photosensitizers on the Antimicrobial Property of Polyurethane coated Leather

  • Oh, Kyung Wha;Lim, Ki Sub
    • Fashion & Textile Research Journal
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    • v.15 no.4
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    • pp.630-634
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    • 2013
  • Cow leather coated with polyurethane film that contains various organic photosensitizers was investigated to demonstrate the antimicrobial properties in the application of the material to protective clothing and home appliances. To prepare the antimicrobial coating on leather surfaces with high potency against microbes, photoactive agents, such as benzophenone (BP), 4,4'-bis(dimethylamino) benzophenone (MK), 4,4'-dihydroxybenzophenone (DHBP) and methylene blue (MB), were incorporated into polyurethane-based coating solutions. The photoactive antimicrobial agent treated leather samples were characterized by SEM, color appearance, color fastness against abrasion, and antimicrobial tests. The optical properties of organic photosensitizers indicated that active UV absorbance ranges were different: BP (around 250 nm), MK (around 360 nm), DHBP (around 305 nm) and MB (around 295 nm &570 nm-685 nm). The intensity of the UV absorbance curve at the UVA light wavelength for the antimicrobial test showed the highest value with MK; subsequently, this was followed by MB, DHBP and BP in decreasing order. The treated-leather samples demonstrated excellent antibacterial activity under UVA light. The antimicrobial effects for the Staphylococcus aureus were superior to Escherichia coli. Moreover, the polyurethane finishing showed an effective durability to abrasion. The overall results indicated that DHBP is the most suitable PU coating additive to provide antimicrobial properties to leather as well as color and surface appearance than MK, MB, and BP.

Evaluation of Self-Cleaning Performance of Mortar Using Nano-Titanium Dioxide Photocatalytic Coating (나노 이산화티탄 광촉매 코팅을 적용한 모르타르의 자기정화성능 평가)

  • Yang, In-Hwan;Jeon, Hyo-Jin;Lee, Seung-Jin
    • Journal of the Korean Recycled Construction Resources Institute
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    • v.10 no.1
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    • pp.56-65
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    • 2022
  • This paper aims at estimating self-cleaning performance of mortar coated with photocatalytic suspension under various conditions. Experimental variables included the concentration (1.5 % and 3.0 %) of photocatalytic suspension for coating mortar specimen, the presence of hydrophilic agent in photocatalytic suspension, and applying the primer on the surface of mortar. The color change of methylene blue solution increased and accordingly self-cleaning performance increased as photocatalytic concentration increased. The presence of hydrophilic agent in photocatalytic suspension slightly decreased the self-cleaning performance compared to the conventional photocatalytic suspension. Test results also showed that mortar specimen including primer and specimen not including primer did not show significantly different self-cleaning performance. In addition, cracks on the surface of mortar specimens decreased as the photocatalytic concentration increased. Therefore, increase in cracks on the surface of mortar at different photocatalytic concentration might adversely affect the self-cleaning performance of mortar specimens.

Surface roughness and color stability of various composite resins (수종의 복합 레진의 표면 거칠기와 색 안정성)

  • Lee, Sung-Yi;Kim, Hyeon-Cheol;Hur, Bock;Park, Jeong-Kil
    • Restorative Dentistry and Endodontics
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    • v.32 no.6
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    • pp.542-549
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    • 2007
  • The purpose of this study was to evaluate the difference in the surface roughness after polishing and to evaluate the difference in color stability after immersion in a dye solution among four types of composite resin materials. Four light-polymerized composite resins(Shade A2) with different sized filler content(a nanofilled, a hybrid, a microfilled, a flowble) were used. Average surface roughness (Ra) was measured with a surface roughness tester (Surftest Formtracer) before and after polishing with aluminum oxide abrasive discs(Super-Snap). Color of specimens before and after staining with 2% methylene blue solution were measured using spectrophotometer(CM-3700d) with SCI geometries. The results of Ra and ${\Delta}E$ were analyzed by one-way analysis of variance(ANOVA), a Scheffe multiple comparison test and Student t-test(p=0.05). After polishing, Ra values were decreased regardless of type of composite resins. In surface roughness after polishing and color stability after staining, nanofilled composite resin was not different with other composite resins except flowable resins.

COMPARISON OF THE SEALING ABILITY OF VARIOUS RETROGRADE FILLING MAIERIALS (수종의 역충전 재료의 치근단 밀폐력 비교)

  • 황윤찬;강인철;황인남;오원만
    • Restorative Dentistry and Endodontics
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    • v.26 no.5
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    • pp.379-386
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    • 2001
  • This study was performed to evaluate the sealing ability of various retrograde filling materials by using bacterial penetration and dye penetration test. One hundred and forty extracted human teeth with single, straight canals and mature apiece were collected and used for this study. All canals were instrumented using an engine driven Ni-Ti file (ProFile). After removing 3mm from the apex of tooth, a standardized 3mm root end cavity was prepared using an ultrasonic instrument. The 70 teeth were randomly divided into 7 groups : 6 groups for retrograde filling using Super-EBA, ZOE, Chelon-Silver, IRM, ZPC and amalgam. The 7th group was used as a negative control. Nail varnish was applied to all external root surfaces to the level of the reseated root ends to prevent lateral microleakages. The specimens were then sterilized in an ethylene oxide sterilizer for 24 hours. 2 mm of the reseated root was immersed in a culture chamber containing a Tripticase Soy Broth with a phenol red indicator. The coronal access of each specimen was inoculated every 72 hours with suspension of Proteus vulgaris. The culture media were observed every 24hours for color change indicating bacterial contamination. The specimens were observed for 4weeks. The remaining 70 teeth were submitted to a dye penetration test. The canals of all teeth were first sealed with AH26 and obturated using an Obtura II system. Root resection, root end preparation and retrograde filling was performed as above. All specimens were suspended in 2% methylene blue dye for 72 hours before being ion gitudinally split. The degree of dye penetration was then measured using a stereomicroscope at 10 magnification and evaluated. The results were as floows : 1. In the bacterial penetration, the degree of leakage was the lowest in the Super-EBA, followed by, in ascending order, ZOE, Chelon-Silver IRM and ZPC. The amalgam showed highest bacterial leakage of all(p<0.01). 2. In the dye penetration, the degree of microleakage was the lowest in the Chelon-Silver and Super-EBA, followed by, in ascending order, IRM, ZPC. The ZOE and amalgam showed the highest microleakage of all (p<0.05). These results suggested that the eugenol based cement, Super-EBA, have excellent sealing ability as a retrograde filling material.

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