• Journal of the Korean Society of Food Science and Nutrition
• /
• v.44 no.10
• /
• pp.1504-1509
• /
• 2015

The aim of this study was to investigate a validation method for determination of pectolinarin and pectolinarigenin in fermented Cirsium setidens Nakai. For validation, the specificity, linearity, precision, accuracy, detection limits, and quantification limits of pectolinarin and pectolinarigenin were measured by HPLC. The results show that the detection limits of pectolinarin and pectolinarigenin were $4.25{\mu}g/mL$ and $2.46{\mu}g/mL$, respectively. The recovery rates of pectolinarin and pectolinarigenin were high in the ranges of 99.7~104.0% and 99.7~102.4%, respectively. Inter-day and intra-day precisions of pectolinarin and pectolinarigenin in fermented Cirsium setidens Nakai were 0.9%, 0.5% and 0.5%, 0.2%, respectively. Therefore, application of pectolinarin and pectolinarigenin was validated by an analytical method as a marker compound in Cirsium setidens Nakai.

• Analytical Science and Technology
• /
• v.28 no.5
• /
• pp.309-316
• /
• 2015

The analytical method of lead in plasma by ICP-MS was validated after securing environment within class 1,000 classification. We tested specificity and accuracy of within-run and between-run. According to measurement of the amount of suspended particulates in a clean room, 0.3~62 particles were detected in 0.3 µm size while 0.0~28.3 particles were observed in 0.5 µm size. Total suspended particulates met required environment with up to 90.3 particles. The MDL (Method detection limit) of the sample which has been fabricated using fetal bovine serum (FBS) blank was 1.77 ng/L, and LOQ (Limit of quantification) was 5.55 ng/L. The slope, intercept and correlation coefficient of the calibration curve were y=1.09×10⁻³x+4.88×10⁻² and r=0.9999, which showed good correlation. The specificity, within-run and between-run accuracy satisfied the standard at more than 50 ng/L. The average lead concentration in plasma of the general people, current workers and retired workers was 55.4 ng/L, 440 ng/L, and 132 ng/L.

• Advances in aircraft and spacecraft science
• /
• v.5 no.2
• /
• pp.251-258
• /
• 2018

This work illustrates the progress of a TAS activity at exploring the challenges and the benefits of the Virtual Shaker Testing (VST) approach. The definition and the validation of new computational methodologies with respect to the state of the art were encouraged throughout this activity. The shaker Finite Element (FE) model in lateral configuration was built for the purpose and it was merged with the SpaceCraft (S/C) FE model, together with the S/C-Shaker adapter. FE matrices were reduced through the Craig-Bampton method. The VST transient analysis was performed in MATLAB(R) numerical computing environment. The closed-loop vibration control is accounted for and the solution is obtained through the fourth-order Runge Kutta method. The use of pre-existing built-in functions was limited by authors with the aim of tracing the impact of all the problems' parameters in the solution. Assumptions and limitations of the proposed methodology are detailed throughout this paper. Some preliminary results pertaining to the current progress of the activity are thus illustrated before the conclusions.

• Transactions on Electrical and Electronic Materials
• /
• v.17 no.5
• /
• pp.252-256
• /
• 2016

This paper deals the study of a linear MHD pump solution used to eliminate and to avoid the dangers of the mercury appearing through pollution and contamination. The formulation of the magnetohydrodynamic phenomena is derived from Maxwell and Navier-Stokes equations are solved using the finite volume method. Simulation results highlight the performance of the pump such as the electromagnetic force, the velocity, and the pressure, the application of Ansys-Fluent software validation these results.

• Wind and Structures
• /
• v.5 no.2_3_4
• /
• pp.317-328
• /
• 2002

A numerical investigation on the turbulent flows over a three-dimensional steep hill is presented. The numerical model developed for the present work is based on the finite volume method and the SIMPLE algorithm with a non-staggered grid system. Standard $k-{\varepsilon}$ model and Shih's non-linear model are tested for the validation of the prediction accuracy in the 3D separated flow. Comparisons of the mean velocity and turbulence profiles between the numerical predictions and the measurements show good agreement. The Shih's non-linear model is found to predict mean flow and turbulence better than the Standard $k-{\varepsilon}$. Flow patterns have also been examined to explain the difference in the cavity zone between 2D and 3D hills.

• Structural Engineering and Mechanics
• /
• v.18 no.5
• /
• pp.691-707
• /
• 2004

This paper presents the micro-mechanical modeling for predicting concrete behavior under compressive loading. The model is able to represent the heterogeneities in the microstructure up to three phases, i.e., aggregate particles, matrix and interfaces. The smeared crack concept based on non-linear fracture mechanics is implemented in order to formulate the constitutive relation for each component. The splitting tensile strength is considered as a fracture criterion for cracking in micro-level. The finite element method is employed to simulate the model based on plane stress condition by using quadratic triangular elements. The validation of the model is verified by comparing with the experimental results. The influence of tensile strength from both aggregate and matrix phases on the concrete compressive strength is demonstrated. In addition, a guideline on selecting appropriate tensile strength for each phase to obtain specified concrete compressive strength is also presented.

• Journal of Power Electronics
• /
• v.14 no.5
• /
• pp.859-865
• /
• 2014

This study presents an electromagnetic model for predicting shaft voltages in a 2-pole field-excited synchronous generator. After the first observations on shaft voltages were made more than a century ago, extensive work has been conducted on eliminating, mitigating, and integrating the aforementioned phenomena. Given that emphasis has been placed on modeling shaft- and bearing-induced voltages in AC motors driven by variable frequency drives, similar efforts toward a model that is dedicated to generators are insubstantial. This work endeavors to improve current physical interpretation and prediction methods for shaft-induced voltages in generators through semi-analytical derivation. Aside from the experimental validation of the model, investigations regarding the behavior of shaft voltages under varying machine complexities and operating conditions clarify previous uncertainties regarding these phenomena. The performance of the numerical method is also assessed for application in eccentricity fault diagnosis.

• Structural Engineering and Mechanics
• /
• v.59 no.5
• /
• pp.921-932
• /
• 2016

This paper presents a theoretical analysis for determining the transverse deflection of simply supported battened beams subjected to a uniformly distributed transverse quasi-static load. The analysis considers not only the shear effect but also the discrete effect of battens on the transverse deflection of the battened beam. The analytical solution is obtained using the principle of minimum potential energy. Numerical validation of the present analytical solution is accomplished using finite element methods. The present analytical solution shows that the shear effect on the transverse deflection of battened beams increases with the cross-section area of the main member but decreases with the cross-section area of the batten. The longer the battened beam is, or the larger the moment of inertia of the main member is, the smaller the shear effect will be.

• Mass Spectrometry Letters
• /
• v.5 no.3
• /
• pp.79-83
• /
• 2014

Three different dutasteride extraction methods were compared and a method based on liquid-liquid extraction (LLE) using methyl tert-butyl ether and methylene chloride was proved to be more effective than others for the extraction of dutasteride and finasteride, the internal standard (IS), from rat plasma. Additionally, a method composed of the LLE extraction, liquid chromatography, and multiple reaction monitoring (MRM) to target dutasteride and IS was validated by assessing specificity, linearity ($r^2$ = 0.9993, 5 - 400 ng/mL), sensitivity (the limit of detection: 4.03 ng/mL; the limit of quantitation: 12.10 ng/mL), accuracy (intra-day: 89.4 - 105.9%; inter-day: 84.9 - 100.9%), precision (intra-day: 0.8 - 6.9%; inter-day: 2.9 - 15.9%), and recovery (84.7 - 107.8%). Since the validated method was successfully applied to a pharmacokinetic study of dutasteride, it can be useful for the pharmacokinetic evaluation of newly developed dutasteride formulations.

• International Journal of Naval Architecture and Ocean Engineering
• /
• v.7 no.5
• /
• pp.920-938
• /
• 2015

In the present study, a numerical prediction method on the hydrodynamic interaction force and moment between two ships in shallow and restricted waterway is presented. Especially, the present study proposes a methodology to overcome the limitation of the two dimensional perturbation method which is related to the moored-passing ship interaction. The validation study was performed and compared with the experiment, firstly. Afterward, in order to propose a methodology in terms with the moored-passing ship interaction, further studies were performed for the moored-passing ship case with a Reynolds Averaged Navier-Stokes (RANS) calculation which is using OpenFOAM with Arbitrary Coupled Mesh Interface (ACMI) technique and compared with the experiment result. Finally, the present study proposes a guide to apply the two dimensional perturbation method to the moored-passing ship interaction. In addition, it presents a possibility that the RANS calculation with ACMI can applied to the ship-ship interaction without using a overset moving grid technique.