• 환경위생공학
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• 제7권1호
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• pp.45-56
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• 1992

The Anaerbic Anoxic/oxic process is one of the biological treatment methods to remove nitrogen and phosphorus effectively which are nutritional elements for eutrophication. Supernatant of primary sediment of Anaerobic digester is used as a carbon source instead of methanol methanol supply in usual A$_{2}$/O process. The efficiency of the following treatment processes are as follow : 1) Changing recycle ratio in the usual A$_{2}$/O process without the stage of Anaerobic digester. 2) Changing recycle ratio in the usual A$_{2}$/O process with the supernatant supply of the Anaerobic digester. In the result of comparison, changing recycle ratio is almost no effect in the removal of phosphorus, however the effect of removal in nitrogenous substance are remarkable, and the effect of Anaerobic digester is not as effective as expected because the BOD removed in the digester partly, the rate of phosphorus to the BOD exceed pertinent range.

• 대한본초학회지
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• 제32권5호
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• pp.13-18
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• 2017

Objectives : The aim of this study is to investigate anti-inflammation of Leonurus sibiricus methanol extract against UVB-damage in fibroblast. The skin is continuously exposed to damage from environmental stresses. UV radiation causes a variety of biological effects especially on the skin, including inflammation and photoaging. Methods : In this study, we tried to search for Leonurus sibiricus which exhibit protective activities against UVB-induced cytotoxicity and oxidative cell death, NO and $PGE_2$ production. HS68 cells were exposed to UVB ($120mJ/cm^2$) and treated with various concentrations (0, 0.25, 0.5, 1, 2, 4, $8mg/m{\ell}$) of Leonurus sibiricus methanol extract for additional 24 h. Intracellular reactive oxygen species (ROS) levels generated by UV radiation were detected using a spectrofluorometer after DCF-DA staining. Also, HS68 cells were irradiated with UVB and then treated with Leonurus sibiricus methanol extract for 12 h. The lipid peroxidation was assayed by measuring the levels of 8-isoprostane secreted into the culture medium. Results : UVB-induced cytotoxicity and cell death were effectively suppressed by treatment of Leonurus sibiricus aqueous methanol extracts. Oxidative cell damage was mediated $PGE_2$ in UVB-induced HS68 fibroblast cell, which was significantly inhibited by treatment with Leonurus sibiricus extracts. Also, the protective effect of these extract seemed to be mediated by inhibited intracellular ROS generation and lipid peroxidation in dose-dependent manner. Conclusion : These results suggest that Leonurus sibiricus aqueous methanol extracts may have anti-aging effects new functional materials against oxidative UVB stress-mediated skin damages.

• 대한임상독성학회지
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• 제21권2호
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• pp.143-150
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• 2023

Purpose: This study aimed to compare the clinical features of methanol and ethylene glycol poisoning. Methods: This single-center retrospective observational study included patients with toxic alcohol poisoning who visited a regional emergency medical center. Patients with methanol and ethylene glycol poisoning from January 2004 to June 2023 were selected for the study using diagnostic codes. Results: Twenty-two patients with toxic alcohol poisoning visited during the study period, with 11 patients for each category. Compared to methanol poisoning, ethylene glycol poisoning patients were more likely to have consumed alcohol for suicidal purposes (n=4 [36.36%] vs. n=8 [72.73%]) and were more likely to be drowsy (n=0 vs. n=6 [54.55%], p=0.016). The anion gap (25.43±8.35 mmol/L vs. 13.22±6.23 mmol/L, p=0.001) and lactic acid levels (1.785 [1.3-2.785] mmol/L vs. 9.90 [4.20-11.81] mmol/L, p=0.007) were higher in ethylene glycol poisoning patients than in methanol poisoning patients. Among alcohol dehydrogenase blockers, oral ethanol was administered to 10 patients (45.45%) (n=4 [36.36%] vs. n=6 [54.55%]), and intravenous ethanol was administered to six patients (n=4 [36.36%] vs. n=2 [18.18%]). Fomepizole was administered to two patients (9.09%) each, and renal replacement therapy was non-significantly more common in patients with ethylene glycol poisoning (n=8 [72.73%] vs. n=3 [27.27%], p=0.128). Three patients had delays in diagnosis and treatment, and while there were no fatalities, one patient was left with permanent vision damage. Conclusion: Because these are uncommon types of poisoning and the clinical presentation is difficult to recognize early, healthcare providers should be familiar with toxic alcohol types and screen for them to ensure proper diagnosis and treatment.

• 한국식품영양학회지
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• 제10권3호
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• pp.382-386
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• 1997

양파 가수분해물의 갈색화는 비효소적 갈색화로 양파에 존재하는 페놀성 물질이 관여하는 듯하므로 이를 확인하고자 여러 침지용매를 이용하여 페놀성 물질의 제거와 갈색도의 관계를 검토하였다. 메탄올, 에탄올 등 용액에 양파를 2시간 침지 후 침지액에서의 페놀성 물질의 함량은 메탄올과 에탄올의 침지액에서 25.1mg/ml과 24.9mg/ml의 페놀성 물질이 제거되었으며, 420nm에서 흡광도는 0.26과 0.22로 물을 이용한 경우보다 높았다. 침지처리 후 제조한 양파 가수분해물의 L값은 메탄올과 에탄올 처리시 81.92와 75.96으로 높은 값을 보였으며, 적색도의 지표인 a값은 물로 침지한 경우 16.41로 높은 값을 보였다. 에탄올의 농도에 따른 갈색화 억제 효과는 80% 이상의 농도를 가진 에탄올 처리시 많은 양의 페놀성 물질이 제거되었으며, 420nm에서 측정한 흡광도 역시 에탄올의 농도가 증가할수록 감소되는 경향을 보였다. L값과 b값 역시 에탄올의 농도가 증가할수록 감소되는 경향을 보였으나, 적색의 지표인 a값은 증가하는 경향을 보였으나, 적색의 지표인 a값은 증가하는 경향을 보였다. 80% 에탄올에 2시간 침지처리하여 만든 양파 가수분해물가 갈색화 억제제로 알려진 시스테인을 0.1% 첨가한 양파 가수분해물의 갈색도를 비교한 결과, 에탄올 침지처리 후 제조한 양파 가수분해물의 갈색도가 현저히 낮았다.

• 대한예방한의학회지
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• 제4권1호
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• pp.81-90
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• 2000

This study was conducted to investigate the antitoxic component in methanol extract of Houttuynia cordata $T_{HUNB}$. The results were as follows: Generally, detoxication effects by Houttuynia cordata $T_{HUNB}$ methanol extract increased in proportion to the methanol extract concentrations. When 40 mg/kg dosage of Houttuynia cordata $T_{HUNB}$ methanol extract was administrated, it showed the highest antitoxic effects in metallothionein induction. After the methanol extract treatment. Body weights increased in proportion to methanol extract concentrations. However, after 3 weeks, the body weight decreased insignificantly. From the above results, these results suggest that the methanol extract of Houttuynia cordata $T_{HUNB}$ increased metallothionein concentration and decreased the toxicity of cadmium in rats.

• 한국토양비료학회지
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• 제43권4호
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• pp.514-521
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• 2010

지속가능한 친환경농업을 위해서는 비료와 함께 식물생장촉진 미생물 또는 생장조절물질을 적절히 혼합 사용하는 것이 바람직하다. 본 연구에서는 Methylobacterium oryzae CBMB20의 근권토양접종과 메탄올 엽면살포에 따른 고추의 생육촉진 효과를 유기질 비료의 시용 수준별로 조사하였다. M. oryzae CBMB20의 근권토양접종과 메탄올 엽면살포는 각기 고추생육을 증대시켰다. 또한 이들을 동시에 혼합 처리하였을 경우에는 고추의 생장촉진 효과가 더욱 현저하게 나타났다. M. oryzae CBMB20의 근권토양접종과 메탄올 엽면살포에 따른 고추 생장촉진 효과는 유기질 비료의 시용수준이 낮은 경우에 더욱 현저하게 나타났다. M. oryzae CBMB20와 메탄올을 혼합 처리한 경우 권장시비수준 100%와 300% 처리 사이에 고추생육과 수량에서 유의성 있는 차이가 없었다. 시비량이 지나치게 많을 경우 생장촉진제의 처리효과를 거둘 수 없으며, 적정한 시비 수준에서 M. oryzae CBMB20와 메탄올을 혼합 처리함으로써 고추를 비롯한 작물의 생장과 수량을 유지하면서도 시비량을 크게 줄일 수 있을 것으로 판단된다.

• 생약학회지
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• 제34권1호통권132호
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• pp.70-74
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• 2003

Cornis fructus has been traditionally used for treating allergy, asthma, hepatitis, and chronic nephritis. It major chemical constituents have been saponins, phenolic acids and loganin. The main aim of the present study was to examine the effect of methanol extract from Cornis fructus on melanogenesis. Cell were cultured in the presence of methanol extracts from Cornis fructus for 48 h, and there were estimated total melanin content as a final product and activity of tyrosinase, a key enzyme, in melanogenesis. Methanol extract from Cornis fructus increased the melanin content and tyrosinase activity in a dose-dependent manner. Particularly, it was observed that only methanol extract $200\;{\mu}g/ml$ stimulated the melanin secretion in B16/F10 melanoma cells by 152% at 48 h treatment and the activity of tyrosinase was increased by 261% in the presence of same concentration.

• 동의생리병리학회지
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• 제25권4호
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• pp.614-619
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• 2011

Nelumbo nucifera(NN) is a oriental medicinal herb which has been used traditionally for the treatment of antidiarrhea, sedative action and various brain diseases including convulsion and epilepsy. In order to examine the mechanism of anticonvulsive effect, we treated the methanol extract of NN(100, 200 mg/kg, P.0) to the sleeping time and pentylenetetrazole(PTZ)-induced convulsive mice. The methanol extract of NN prolonged sleep time by pentobarbital. Methanol extracts of NN were not effected the concentration of GABA and GABA-T activity in the brain of PTZ-induced mice. Methanol extracts of NN significantly inhibited the convulsion state as well as the level of lipid peroxidation in the brain. The butanol and dichloromethane fraction of methanol extracts among the others effectively inhibited in vitro lipid peroxidation dose dependently($5.0{\times}10^{-6}\sim2.0{\times}10^{-5}\;g/ml$). These results suggest that the anticonvulsive effect of NN is possibly due to the antioxidative effects of the free radical formation at brain for the PTZ-induced convulsion if it were by due to generating system.

• 대한한방피부미용학회지
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• 제1권1호
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• pp.41-52
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• 2005

The aim of this study was to investigate the effect of Fructus Forsythiae on the melanogenesis. To determine whether Fructus Forsythiae methanol extract suppress melanin synthesis in cellular level, HM3KO human melanoma cells were cultured in the presence of various concentrations of Fructus Forsythiae methanol extract and the effects on cell proliferation, tyrosinase activity and melanin contents were examined. Treatment with Fructus Forsythiae methanol extract inhibited tyrosinase activity, regulate melanin biosynthesis as the key enzyme in melanogenesis, in a dose-dependent manner. And also suppressed melanin contents as a dose dependent manner without cytotoxicity morphological change. It was observed that the color of cell pellets was totally different from the control. These results suggest that the inhibitory effect of Fructus Forsythiae methanol extract on melanogenesis is due to the suppression of tyrosinase in HM3KO cells and Fructus Forsythiae is a candidate for an efficient whitening agent.

• 한국식품과학회지
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• 제39권6호
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• pp.701-707
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• 2007

민간요법에서 항당뇨 및 항비만 효과가 있는 것으로 알려진 길경의 항당뇨 효과가 있는 지 여부를 in vitro에서 조사하기 위해서 길경을 70% 에탄올로 추출한 후 메탄올과 물을 섞은 용액으로 단계별로 XAD-4 column으로 분획하였다. 본 연구에서는 1) 3T3-L1 섬유아세포와 지방세포에서 길경의 추출 분획물이 인슐린처럼 작용하는 인슐린성 물질이거나, 2) 인슐린 작용을 향상시키는 인슐린 민감성 물질이거나, 또는 3) 포도당 자극에 의한 인슐린 분비를 향상시키거나, 4) 베타세포의 기능과 양을 증가시키는데 관여하는 유전자인 IRS-2, glucokinase, PDX-1의 mRNA 발현을 향상시키거나, 5) $\alpha-glucoamylase$ 활성을 억제하는 물질로 작용하는 지 여부를 조사하였다. 길경 추출 분획물은 인슐린성 물질로 작용하지 않았다. 반면에 0, 20와 100%메탄올층은 3T3-L1 지방세포에서 인슐린 자극에 의한 포도당 흡수를 증가시켰다. 이 분획층 중에서 특히 0%과 100% 메탄올 분획층은 분화 유도물질의 작용을 향상시켜 3T3-L1 섬유아세포에서 지방세포로의 분화 및 중성 지방의 축적을 증가시켰다. 그러므로 이들은 $PPAR-{\gamma}$ agonist로 작용하는 물질을 함유할 가능성이 매우 높다. 인슐린을 분비하는 세포인 Min6 세포에서 포도당 자극에 의한 인슐린 분비를 향상시키는 지 여부를 조사하였는데 20, 80 그리고 100% 메탄올층은 포도당 자극에 의한 인슐린 분비를 증가시켰다. 그 기전은 인슐린 분비와 베타세포의 증식에 관여하는 유전자의 IRS-2, glucokinase 그리고 PDX-1의 mRNA의 양을 증가시키는 것과 관련이 있다. 결론적으로 길경은 지방 세포의 분화를 촉진하는 물질, 인슐린 민감성을 향상시키는 물질 그리고 베타세포의 기능과 증식을 촉진시키는 물질을 함유하고 있으므로 우리나라 및 아시아의 사람들에서 많이 유발되는 비만을 동반하지 않은 당뇨병 및 인슐린 저항성의 치료와 예방에 중요한 역할을 할 것으로 사료된다.