• Korean Journal of Medicinal Crop Science
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• 제13권2호
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• pp.110-114
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• 2005

Extracts from sawdust of Pinus densiflora were showed antifungal activity against Lentinula edodes. It was extracted by hot water and then successively extracted by n-hexane, ethyl acetate, and methanol. The yields of the n-hexane-soluble, ethyl acetatesoluble, methanol-soluble and methanol-insoluble fractions of water extracts were 8.2%, 10.6%, 32.0%, and 49.2%, respectively. The ethyl acetate-soluble fraction showed the greatest antifungal activity against L. edodes: 41.5% inhibition at 1,000 ppm. However, there were not significant differences of antifungal activities between n-hexane-soluble fraction and methanol-soluble fraction at a concentration of 1,000 ppm. The hot water extracts showed 23.5% of antifungal activity against L. edodes at a concentration of 1000 ppm. The four antifungal compounds were separated from ethyl acetate fraction by thin layer chromatography.

• Toxicological Research
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• 제12권1호
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• pp.41-46
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• 1996

This study was performed to investigate the antitoxic component in methanol fraction of Lonicerae fios. The results were as follows: 1. When a 500 ppm of water soluble fraction of Lonicerae flos was administered, it showed the highest antitoxic effect. 2. Generally, detoxication effects by methanol fraction of Lonicerae fios increased. When the ethyl acetate soluble fraction of Lonicerae rios was administered, it exhibited the highest antitoxic effect against the toxicity of cadmium in Liver. 3. The combined administration of cadmium and methanol fraction of Lonicerae rios significantly increased metallothionein in liver compared to administration of cadmium only. This phenomenon was more remarkable when the ethyl acetate soluble fraction of Lonicerae fios was administered with cadmium chloride.

• The Journal of Korean Medicine
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• 제23권3호
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• pp.223-232
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• 2002

Objectives : Methicillin-resistant Staphylococcus aureus (MRSA) CCARM 3251 and S. aureusKCTC 1928 have been known to be resistant to many kinds of antibiotics. The extract of Glycyrrhizae Radix showed antibacterial activity against MRSA and antibiotics-resistant S. aureus. Methods : We examined the effects of the water-soluble extract and the methanol-soluble extract of Glycyrrhizae Radix on MRSA and antibiotic-resistant S. aureus. The methanolic extract was further fractionated with organic solvents such as hexane, chloroform, and ethyl acetate in that order. Results and Conclusions : The methanol-soluble extract of Glycyrrhizae Radix showed relatively high antibacterial activity against MRSA and antibiotic-resistant S. aureus. However, the water-soluble extract of Glycyrrhizae Radix showed no antibacterial activity against MRSA and antibiotic-resistant S. aureus. Among the fractions tested, the chloroform fraction showed the highest antibacterial activity against MRSA and antibiotic-resistant S. aureus. The methanol-soluble extract of Glycyrrhizae Radix minimal inhibitory concentrations (MICs) against MRSA and antibiotics-resistant S. aureus were $5{\;}mg/m{\ell}$ in both. The methanol-soluble extract of Glycyrrhizae Radix was separated using thin-layer chromatography and detected with UV -detector. Further study should be carried out to identify which effects cell growth inhibition of MRSA and antibiotics-resistant S. aureus.

• Journal of Korean Society of Water and Wastewater
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• 제19권4호
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• pp.446-454
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• 2005

An economic treatment method to remove oxidized nitrogen from wastewater is biological denitrification with organic matters. Several organics can be used, however, methanol is commonly used. When methanol is provided, M:N (Methanol to Nitrogen) ratio is used to define methanol demand for denitrification. In this study, two artificial wastewaters were provided to a biological system to evaluate denitrification performance. Differences of influent total CODcr from effluent soluble CODcr were converted to methanol equivalent and oxidized nitrogen difference between influent and effluent were converted to nitrate equivalent to define M:N ratios. Modes I, II, III, I-1 and IV showed 5.1, 2.7, 3.3, 2.3 and 2.6 of M:N ratios, respectively. Since denitrifying microorganisms had to build a new metabolic system for methanol and influent organics, initial operation mode, Mode I, required more methanol and this resulted in high M:N ratios compared with later operation mode, Mode I-1. Salt in influent did not show inhibitory effects on denitrfication, although this was believed to increase effluent SS and soluble CODcr concentrations in Mode III, I-1 and IV, respectively. The concentrations of effluent soluble $COD_{Mn}$ did not changed much with influent salt.

• Journal of Environmental Science International
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• 제20권10호
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• pp.1265-1272
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• 2011

In this study, the extraction characteristics of soluble solid from Rumex crispus(Curled dock) was studied from the investigation of the effects of experimental conditions on extraction rate; extraction ratio, composition of extractants, extraction time and pH of extractant, etc. The proximate composition of Rumex crispus was 2.58% crude lipid, 5.59% crude protein, 7.39% crude ash, 6.13% moisture and 78.31% carbohydrate, respectively. Turbidity of extract by distilled water was higher and increased with extraction time and extraction temperature, where as the turbidity didn't increase by ethanol and methanol in 20 folds of extraction ratio. Turbidity was inversely proportional to the extraction ratio for the three extractants at 25$^{\circ}C$ and 1 hour extraction. But turbidity of extract was highest by composition of 50% methanol-water extractant than any other compositions of extractants. Eighteen and fifteen free aminoacids were detected in extracts with distilled water, methanol and ethanol extractant, respectively, and it's contents were order of glutamic acid>proline>aminobutyric acid>alanine. The extraction rate of soluble solid from Rumex crispus was order of distilled water>methanol>ethanol within experimental extraction ratio. In extraction with distilled water, the contents of soluble solid was inversely proportional to the pH of extractant.

• Journal of Pharmaceutical Investigation
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• 제29권1호
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• pp.55-60
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• 1999

We previously showed that a methanol extract of Paeoniae radix decreased total cholesterol level in rats with hyperlipidemia. In order to isolate the active ingredient(s), the methanol extract of Paeoniae radix was fractionated with chloroform/methanol(4:1) solution and isolate into soluble part and insoluble part of the the methanol extract. Above two parts were tested on the experimentally induced hypercholesterolemia in rats for the lowering effect of serum lipoprotein contents. Hyperlipidemia was induced on male Wistar rats by feeding high choleserol diet for 7 days. After oral administration of above samples for 4 weeks, serum lipid profile was verified on these rats by measuring total cholesterol, triglyceride, high density lipoprotein cholesterol and low density lipoprotein cholesterol. The chloroform/methanol(4:1) soluble part and insolule part showed lowering activity of total cholesterol level and triglyceride level at 4 week point significantly(p<0.01 and p<0.05) compare with the control group and the soluble part was more effective.

• Journal of the Korean Society of Food Science and Nutrition
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• 제35권8호
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• pp.1097-1101
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• 2006

In this study, seven species of seaweeds (Sargassum horneri, Grateloupia filicina, Kjellmaniella crassifolia, Porphyra tenera, Ecklonia stolonifera, Scytosiphon lomentaria, Agarum cribrosum) from Gangwon coast were collected for antioxidative assays. The methanol soluble and insoluble (water soluble) fraction which were prepared from 75% methanol extraction were assayed by DPPH method and deoxyribose assay. The antioxidative activity of methanol soluble fraction of Agarum, Scytosiphon and Sargassum reached to 26.60%, 24.28% and 23.40%, respectively. The antioxidative activities of methanol insoluble fractions of the Kjellmaniella and Porphyra were assayed to 29.70% and 21.01% which were higher than methanol soluble fractions with the values of 9.26% and 8.66%, respectively. The results from DPPH assay and deoxyribose assay showed the methanol soluble fractions of Agarum and Sargassum have strong antioxidative activity. However the methanol insoluble (water soluble) fractions of seaweeds did not show any meaningful antioxidant activity excepted Kjellmaniella and Porphyra.

• Journal of the Korean Society of Food Science and Nutrition
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• 제23권2호
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• pp.251-260
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• 1994

Lipophilic brown pigments produced during the fermentation of domestic Meju and Doenjang were fractionated by column and thin -layer chromatography (TLC). Each of the fractions was tested for the antioxidant activity and then characterized by spectroscopic analysis. The lipophilic brown pigments were separated into chloroform -soluble and methanol-soluble parts in which Meju resulted the higher content of chloroform-soluble part than that of methanol-soluble part ; however, Doenjang exhibited the opposite result to that of Meju. More strong antioxidant activity was found in the methanol-soluble part than the chloroform-soluble part. Four and five fractions were separated from chloroform-soluble and methanol-soluble parts respectively. by silicagel TLC. The fraction that exhibited the high antioxidant activity showed a strong absorption at 260nm caused by amino compounds in UV spectrum The other fractions which did not have antioxidant activity absorbed at 240nm by carbonic acid and it ester,. IR spectrum of each fraction commonly showed absorption at 3400cm-1 , 2800cm-1 , 1700cm-1, 1600cm-1, 1400cm-1 , 1300cm-1 and 1100cm-1. Especially , the fraction which had a strong antioxidant activity showed absorption at 2800cm-1, 1400cm-1, 1600cm-1 suggesting that the fraction contain Schiff's base and primary amine structure.

• YAKHAK HOEJI
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• 제27권3호
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• pp.235-243
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• 1983

In the present study, in attempt was made to observe the effect of lipid components in pantocrin and antler velvet and sponge layers extracts on the aldolase activity in the rat spinal nerves. The antler chloroform-methanol (2:1) extracts are known to be composed of neutral lipids, cholesterol, cerebrosides, spingomyelin, phosphatidyl choline, phosphatidyl ethanolamine, phosphatidyl serine and gangliosides. The antler extracts were studied on the aldolase activity in the rat spinal nerves. The aldolase activity was measured by the method of Lehninger. To investigate the components of tile extracts which affect the enzyme activity, the chloroform-methanol (2:1) extracts of antler were fractionated into petroleum ether soluble and insoluble fractions and their effects on the enzyme activity were compared. It was found that the chloroform-methanol (2:1) extracts of the antler sponge and velvet layers as well as those petroleum ether soluble fractions obtained from the chloroform-methanol (2:1) extracts, decrease the aldolase activity by 18-23%. However, pantocrin showed increasing effect on the aldolase activity by 42.6%. The petroleum ether insoluble fraction of the chloroform-methanol (2:1) -extract showed no significant increasing activity (about 13%) on the spinal nerves aldolase. The components of pantocrin ind the petroleum ether insoluble fractions were attempted to analysize by thin layer (silica gel) and gas liquid chromatography.

• Textile Coloration and Finishing
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• 제14권1호
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• pp.11-17
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• 2002

To identify the antimicrobial activity, of tumeric and its active compound tumeric was fractionated into four groups dichloromethane extract, hexane fraction, methanol soluble fraction and residue's extract. They were tested for antibacterial activity against E. coil and S. aureus and the methanol soluble fraction was found lo be the most active fraction. Compound I, II and III were isolated from TLC and silica gel column chromatography in the methanol soluble fraction. These compounds were analyzed by $^1H-NMR\;and\;^{13}C-NMR$ spectra and identified as curcumin I, II and III. They were also tested for antimicrobial activity against E. coli and S. aureus. Curcumin I was the must active curcuminoids due to the phenolic and methoxyl$(OCH_3)$ moiety in the same molecular structure.