• 한국약용작물학회:학술대회논문집
• /
• 2007.05a
• /
• pp.127-128
• /
• 2007

• Journal of Life Science
• /
• v.11 no.6
• /
• pp.603-611
• /
• 2001

Phytolalexins are produced in plants affected by various environmental factors such as fungal infection treatment with many chemicals and irradiation by ultraviolet light. When pepper and tobacco bel suspension cultures were grown on a basal MS medium supplemented with 2,4-D(1mg/$\ell$, benzyl adenine(0.001 mg/$\ell$) and 100$\mu$ M jasmonic acid, the production of capsidiol was observed. The total of compound found in pepper plant were around seventy and thirty of them were located intissue-specific manner. 1-propanethiol, $\alpha$-D-xylofuranoside, phenol, hexadecanonic acid ethyl tridecanoate, phytol, linoleic acid and capsidiol are those which have change the production level by treatments, such as the inoculation of Phytophthora capsici Leonian, the metalaxyl treatment and the UV-B irradiation, respectively. The content of capsidiol on inoculation of P. capsici with metalxyl suspension in soil were higher than those of P.capsici without metalaxyl. When the soil dernch of metalaxyl treatment (1$\mu\textrm{g}$/${mu}ell$)was delayed after inoculation, the content of capsidiol were higher than that of before. Irrradiated UB-B the production on capsidiol was identified only at leaf, and contents were the highest for 24 hrs incubation after 20 minutes irradiation.

• Journal of Applied Biological Chemistry
• /
• v.65 no.1
• /
• pp.17-21
• /
• 2022

A new rapid and simple method for metalaxyl in Atractylodes macrocephala Koidzumi and Achyranthes japonica Nakai has been developed and validated. This study was conducted to develop a method for analyzing metalaxyl by a method based on QuEChERS using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). Samples were extracted with acetonitrile and purified using amino-propyl (NH₂) Solid Phase Extraction cartridge. The method limit of quantitation (MLOQ) was 0.01 mg/kg. The linearity of matrix-matched calibration curve (r²) was ≥0.99 at the calibration range of 0.001-0.05 mg/kg. For recovery test, Atractylodes macrocephala Koidzumi or Achyranthes japonica Nakai was treated with standard solutions at MLOQ and 10MLOQ levels. Recovery rates were in the range of 88.1-109.1% with <5.5% coefficient of variation. This established analytical method was fully validated. Based on these results, it can contribute to improving the safety of residual pesticides in Atractylodes macrocephala Koidzumi and Achyranthes japonica Nakai.

• The Korean Journal of Pesticide Science
• /
• v.14 no.4
• /
• pp.456-462
• /
• 2010

Baseline sensitivity to benthiavalicarb, iprovalicarb and dimethomorph included into carboxylic acid amide (CAA) group was evaluated in 180 isolates of Phytophthora capsici over 4 years from 2005 to 2008. $EC_{50}$ (effective concentration inhibiting mycelial growth by 50%) value of benthiavalicarb ranged from $0.015{\mu}g\;mL^{-1}$ to $0.049{\mu}g\;mL^{-1}$ with a mean of $0.033{\mu}g\;mL^{-1}$. The mean values of $EC_{50}$ of iprovalicarb and dimethomorph were 0.411 (0.197 - 0.556) ${\mu}g\;mL^{-1}$ and 0.271 (0.101 - 0.798) ${\mu}g\;mL^{-1}$, respectively. Although there was no increasing tendency in $EC_{50}$of benthiavalicarb and iprovalicarb during 4 years, $EC_{50}$ of dimethomorph was increased gradually by laps of time. There was no cross-resistance between each fungicide used in this study and metalaxyl. Among fungicides included into CAA group, there was a positive correlation between benthiavalicarb and iprovalicarb, and between dimethomorp and mandipropamid.

• The Plant Pathology Journal
• /
• v.18 no.3
• /
• pp.147-151
• /
• 2002

Seven fungicides were compared for the control of downy mildew on midge gourd. All treatments had significantly lower rates of disease progress curves and disease severity levels than that of the control. The highest yields were obtained from crops treated with metalaxyl + mancozeb, fosetyl-Al, and chlorothalonil. These treatments also proved to be the most economical considering the treatment costs.

• Korean journal of applied entomology
• /
• v.45 no.2 s.143
• /
• pp.217-226
• /
• 2006

As the results achieved by the evaluation of toxicities on an aphid parasitoid, Aphidius colemani by 79 pesticides registered as horticultural pesticide and 4 adjuvants far pest control, 6 insecticides including a-cypermethrin, 13 fungicides including metalaxyl-M+mancozeb and 4 acaricides including bifenazate showed low toxicity against A. colemani adult. Low toxicity was showed in all the 4 adjuvants as well. In residual toxicity test from 40 pesticides which showed toxicity more than 50%, A. colemani was safe from 11 pesticides from the 3th day after treatment, 7 pesticides from the 5th day after treatment and 14 pesticides from the 7th day after treatment, respectively. But, chlorpyrifos-methyl, diflubenzuron+chlorpyrifos, etofenprox+diazinone and imidachloprid+chlorpyrifos showed high toxicities reaching 100%, 97.7%, 100% and 100% respectively, even from the 7th day after treatment. To evaluate the control effect by A. colemani against Myzus persicae in a greenhouse, A. colemani was released at parasitoid versus aphids rates of 1:50 and 1:100 when the population of M persicae was 50 per plant. After release, aphids population remained steady for 20 days after release at the level of around 60 aphids per plant. During the investigation, insecticides fur thrips control and fungicides for powdery mildew control were treated, but didn't affect the mummy forming of A. colemani. It may be suggested from these results that the selected insecticides, fungicides, acaricides and adjuvent could be incorporated into the integrated M. persicae management system with A. colemani on greenhouse cultivation.

• Journal of the Korean Society of Tobacco Science
• /
• v.13 no.1
• /
• pp.53-60
• /
• 1991

Seedling rot occurred on burley tobacco at the early growing stage after transplanting was surveyed in the Chonbuk province, the main area for burley tobacco production, during the 3 crop years from 1986. The incidence of disease was about 0.6-0.7% every year and major causal organisms were Pythium spp. More diseased plants were observed in the fields in which tobacco planted with improved mulching system than in that with any other mulching system. The temperature and relative humidity in the planting hole covered with polyethylene film for 11 days after transplanting were recorded by 27-45$^{\circ}C$ and 75~95%, respectively. The punched polyethylene film mulching field showed less diseased plants than that with improved mucking. In improved mulching system the longer period of mulching caused higher incidence of seedling rot(r=0.74**). Soil drench treatment of 200m1 of aqueous Metalaxyl Solution(125ppm) was effective to control the disease.

• Korean Journal of Plant Resources
• /
• v.15 no.3
• /
• pp.211-215
• /
• 2002

This study was carried out to select fungicides which are effective for the control of anthracnose disease of Carthamus tintorious L. caused by Cottetotrichum acutatum. Eive chemicals, i.e., thiophanate-methyl$.$triflumizole, iminoctadinetris$.$thiram, metiram, bitertanol$.$propineb, metalaxyl$.$dithianon were treated to Cheongiu native variety for the test of control effect. The results obtained were summarized as the follows; No. of harvested plants per ㎡ was most in iminoctadinetris$.$thiram and its value showed 21.2 compared with 16.8 in control. Iminoctadinetris$.$thiram and metiram were most effective to control the disease and their contyol values were 57.2%, and 49.4%, respectively. Chemical injury of five chemicals was not occurred at the double-diluted solution treatment. Seed yield was 47∼48% higher in iminoctadinetris$.$thiram and metiram than 75 kg/10 a in control.

• Journal of Food Hygiene and Safety
• /
• v.36 no.1
• /
• pp.1-8
• /
• 2021

The inverted Y-shaped strip detection method based on acetylcholinesterase (AChE) was developed for the rapid detection of organophosphorus and carbamate pesticides. The inactivation of AChE by organophosphorus and carbamate pesticides has been well known. The AChE catalyzes acetylthiocholine into thiocholine having (-) and (+) charges, and the (+) charge results in aggregation of gold nanoparticle (GNP). Malaoxon and carbofuran were used as standard organophosphorus and carbamate for the development of the inverted Y-shaped strip, respectively. In order to optimize the method, various angles of the Y-shaped strip, different types of nitrocellulose membrane, and concentration of AChE were tested as key parameters. The detection limit of the method was 10 ng/mL for both malaoxon and carbofuran pesticides. No cross-reaction was observed to other pesticides such as atrazine, cyanazine, simazine, bifenthrin, boscalid, metalaxyl, and chlorobenzilate. Recoveries from lettuce spiked when known concentrations of malaoxon and carbofuran were found ranging from 96.4 to 100.7% and 81 to 112.7%, respectively. This study suggests that the inverted Y-shaped strip method based on AChE may be a useful tool for the sensitive, specific, rapid detection of organophosphorus and carbamate pesticides in agricultural products.