• Biomolecules & Therapeutics
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• 제19권1호
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• pp.38-44
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• 2011

Cisplatin is widely used for various types of cancers. However, its side effects, most notably, renal toxicity often limit its clinical utility. Although previous metabolomic studies reported possible toxicity markers, they used small number of animals and statistical approaches that may not perform best in the presence of intra-group variation. Here, we identified urinary biomarkers associated with renal toxicity induced by cisplatin using NMR-based metabolomics combined with Orthogonal Projections to Latent Structures-Discriminant Analysis (OPLS-DA). Male Sprague-Dawley rats (n=22) were treated with cisplatin (10 mg/kg single dose), and the urines obtained before and after treatment were analyzed by NMR. Multivariable analysis of NMR data presented clear separation between non-treated and treated groups. The OPLS-DA statistical results revealed that 1,3-dimethylurate, taurine, glucose, glycine and branched-chain amino acid (isoleucine, leucine and valine) were significantly elevated in the treated group and that phenylacetylglycine and sarcosine levels were decreased in the treated group. To test the robustness of the approach, we built a prediction model for the toxicity and were able to predict all the unknown samples (n=14) correctly. We believe the proposed NMR-based metabolomics with OPLS-DA approach and the resulting urine markers can be used to augment the currently available blood markers.

• Journal of Ginseng Research
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• 제44권4호
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• pp.544-551
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• 2020

Background: Previous studies have shown the insecticidal efficacy of ginsenosides. In the present study, we aimed to investigate the metabolic mechanism related to the inhibitory effect of panaxadiol saponins (PDSs) against the Asian corn borer Ostrinia furnacalis (Guenee). Methods: Third instar larvae of O. furnacalis were fed normal diets with different concentrations of PDSs for 4 days. The consumption index, relative growth rate, approximate digestibility, and conversion of ingested and digested food were recorded. A targeted gas chromatographye-mass spectrometry assay was performed to detect the profiles of amino acids, fatty acids, and carbohydrates in larvae of O. furnacalis. In addition, the activity of detoxification-related enzymes was determined. Results and Conclusions: PDSs decreased the consumption index, relative growth rate, approximate digestibility, and conversion of ingested and digested food in the 3rd instar larvae of O. furnacalis in a dose-dependent manner. PDSs decreased 15 free amino acids, 16 free fatty acids, and 5 carbohydrates and increased the levels of palmitoleic acid, palmitic acid, and 9-octadecenoic acid in the 3rd instar larvae. The activity of detoxification-related enzymes, such as acetylcholinesterase, glutathione S-transferase, cytochrome P450, carboxylesterase, trehalase, acid phosphatase, and alkaline phosphatase, was reduced in a dose-dependent manner in the 3rd instar larvae exposed to PDSs. These data confirmed the inhibitory effect of PDSs against growth, food utilization, and detoxification in the 3rd instar larvae of O. furnacalis and the potential for using PDSs as an efficient tool for insect pest management for O. furnacalis larvae.

• 한국균학회소식:학술대회논문집
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• 한국균학회 2018년도 춘계학술대회 및 임시총회
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• pp.13-13
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• 2018

Alternaria is a ubiquitous fungal genus, widely distributed in the environment and a range of different habitats. It includes both plant pathogenic and saprophytic species, which can affect crops in the field or cause post-harvest spoilage of plant fruits and kernels. Numerous Alternaria species cause damage to agricultural products including cereal grains, fruits and vegetables, and are responsible for severe economic losses worldwide. Most Alternaria species have the ability to produce a variety of secondary metabolites, which may play important roles in plant pathology as well as food quality and safety. Alternariol (AOH), alternariol monomethyl ether (AME), tenuazonic acid (TeA), tentoxin (TEN) and altenuene (ALT) are considered the main Alternaria compounds thought to pose a risk to human health. However, food-borne Alternaria species are able to produce many additional metabolites, whose toxicity has been tested incompletely or not tested at all. Both alternariols are mutagenic and their presence in cereal grain has been associated with high levels of human esophageal cancer in China. TeA exerts cytotoxic and phytotoxic properties, and is acutely toxic in different animal species, causing hemorrhages in several organs. The possible involvement of TA in the etiology of onyalai, a human hematological disorder occurring in Africa, has been suggested. Altertoxins (ALXs) have been found to be more potent mutagens and acutely toxic to mice than AOH and AME. Other metabolites, such as TEN, are reported to be phytotoxins, and their toxicity on animals has not been demonstrated up to now. Vegetable foods infected by Alternaria rot are obviously not suitable for consumption. Thus, whole fresh fruits are not believed to contribute significantly with Alternaria toxins to human exposure. However, processed vegetable products may introduce considerable amounts of these toxins to the human diet if decayed or moldy fruit is not removed before processing. The taxonomy of the genus is not well defined yet, which makes it difficult to establish an accurate relationship between the contaminant species and their associated mycotoxins. Great efforts have been made to organize taxa into subgeneric taxonomic levels, especially for the small-spored, food associated species, which are closely related and constitute the most relevant food pathogens from this genus. Several crops of agricultural value are susceptible to infection by different Alternaria species and can contribute to the entry of Alternaria mycotoxins in the food chain. The distribution of Alternaria species was studied in different commodities grown in Argentina. These food populations were characterized through a polyphasic approach, with special interest in their secondary metabolite profiles, to understand their full chemical potential. Alternaria species associated with tomato, bell pepper, blueberry, apples and wheat cultivated in Argentina showed a surprisingly high metabolomic and mycotoxigenic potential. The natural occurrence of Alternaria toxins in these foods was also investigated. The results here presented will provide background for discussion on regulations for Alternaria toxins in foods.

• 생명과학회지
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• 제28권10호
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• pp.1244-1253
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• 2018

반추위 속에는 박테리아, 고세균, 프로토조아, 곰팡이 및 바이러스와 같은 다양한 미생물들이 편성의 혐기조건에서 공생하고 있다. 사료의 발효에 중요한 역할을 하고 있는 반추위 미생물은 위내 발효과정에서 에너지 손실에 영향을 주는 메탄의 발생을 제외하면 에너지와 단백질 대사에 필수적인 다양한 휘발성 지방산을 생산한다. 반추위내 미생물의 이용효율을 개선시키기 위해 사료배합비조절, 천연사료첨가제, 생균제첨가 등의 다양한 접근방법들이 사용되고 있다. 최근에 반추위 군집에 대한 메타유전체 또는 메타전사체와 같은 차세대 유전체 해독기술 또는 차세대 시퀀싱 기술의 적용으로 반추위 미생물의 다양성 및 기능에 대한 이해가 크게 증가하였다. 특히 메타단백질체와 메타대사체는 반추위 생태계의 복잡한 미생물네트워크에 대한 더 깊은 통찰력을 제공할 뿐만 아니라, 다양한 반추가축용 사료에 대한 반응을 제공함으로서 생산효율을 개선시키는데 기여하였다. 본 논문에서는 반추위내 사료의 발효와 이용을 향상시키기 위한 메타오믹스 기술, 즉, 메타유전체, 메타전사체, 메타단백질체 및 메타대사체의 최신 응용기술을 요약하고자 한다.

• Journal of Animal Science and Technology
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• 제63권2호
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• pp.319-331
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• 2021

Heat stress (HS) causes adverse impacts on pig production and health. A potential biomarker of HS is required to predict its occurrence and thereby better manage pigs under HS. Information about the saliva metabolome in heat-stressed pigs is limited. Therefore, this study was aimed to investigate the effects of acute HS on the saliva metabolome and identify metabolites that could be used as potential biomarkers. Growing pigs (n = 6, 3 boars, and 3 gilts) were raised in a thermal neutral (TN; 25℃) environment for a 5-d adaptation period (CON). After adaptation, the pigs were first exposed to HS (30℃; HS30) and then exposed to higher HS (33℃; HS33) for 24 h. Saliva was collected after adaptation, first HS, and second HS, respectively, for metabolomic analysis using 1H-nuclear magnetic resonance spectroscopy. Four metabolites had significantly variable importance in the projection (VIP > 1; p < 0.05) different levels in TN compared to HS groups from all genders (boars and gilts). However, sex-specific characteristics affected metabolites (glutamate and leucine) by showing the opposite results, indicating that HS was less severe in females than in males. A decrease in creatine levels in males and an increase in creatine phosphate levels in females would have contributed to a protective effect from protein degradation by muscle damage. The results showed that HS led to an alteration in metabolites related to energy and protein. Protection from muscle damage may be attributed to the alteration in protein-related metabolites. However, energy-related metabolites showed opposing results according to sex-specific characteristics, such as sex hormone levels and subcutaneous fat layer. This study had shown that saliva samples could be used as a noninvasive method to evaluate heat-stressed pigs. And the results in this study could be contributed to the development of a diagnostic tool as a noninvasive biomarker for managing heat-stressed pigs.

• 생물환경조절학회지
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• 제31권4호
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• pp.332-342
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• 2022

케일(Brassica oleracea var. acephala)은 필수 아미노산, 비타민, 미네랄과 같은 수많은 영양소를 함유하고 특히 글루코시놀레이트가 풍부하기 때문에 전 세계적으로 가장 많이 소비되는 잎 채소 중 하나이다. 그러나 케일 품종 간의 글루코시놀레이트 합성과 관련된 유전자 발현에 대한 연구는 미비한 실정이다. 본 연구에서는 전사체 및 대사체 분석을 사용하여 식물공장에서 재배된 녹색(만추 및 맛짱) 및 적색 케일 품종(적곱슬)을 포함한 3 가지 케일 품종에서 글루코시놀레이트를 조사하였다. 재배 후 6주된 녹색 케일 품종의 생육 및 발달이 적색 케일 품종에 비해 높았다. High-performance liquid chromatography (HPLC) 분석에서 7가지 글루코시놀레이트를 분석하였다; 만추 품종에서는 5종의 글루코시놀레이트가, 맛짱과 적곱슬 품종에서는 4종의 글루코시놀레이트가 분류되었다. Glucobrassicin은 3가지 케일 품종에서 가장 높은 글루코시놀레이트 였다. 총 글루코시놀레이트 함량은 적곱슬 품종에서 가장 높았다. 전사체 분석에서는 8개의 유전자가 글루코시놀레이트 합성에 관여됨을 확인할 수 있었다. 이러한 결과는 케일 품종에 따라 글루코시놀레이트 함량과 축적 패턴이 다르다는 것을 시사한다.

• 생명과학회지
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• 제32권5호
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• pp.391-410
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• 2022

인간 미생물 균총은 장, 구강, 피부와 같이 체내외 다양한 부위에 존재하는 박테리아, 균류, 바이러스 등을 포함하는 미생물 집단이다. 16s ribosomal RNA에 대한 대사체 분석 및 차세대 염기서열 분석기술의 개발과 함께, 살아있는 유기체 내에 존재하는 미생물 균총에 대한 많은 연구가 진행되었다. 이에 따라, 미생물 균총이 숙주의 대사 및 면역과정과 복잡하게 연관되어 있음이 확인되었다. 공생균(commensal microbiota)이라 불리는 미생물 균총의 특정 박테리아가 필수 영양소를 생성하거나 다른 병원성 미생물로부터 숙주를 보호하여 긍정적으로 영향을 미치고 있는 반면, 비정상적인 미생물 균총의 조성을 의미하는 미생물 균총의 불균형(dysbiosis)에 의해 체내 항상성 유지를 방해하여 다양한 종류의 질병을 발생시키기도 한다. 최근, 미생물 균총 중에서도 구강과 장내 존재하는 박테리아가 위장관암의 발암과정과 항암제의 치료효과에 상당한 영향을 미치고 있음이 여러 논문을 통해 보고되고 있다. 미생물 균총-암-면역계 사이의 복잡한 연관성과 미생물 균총 기반 발암 메커니즘에 대한 규명은 암에 대한 이해와 새로운 항암제 개발에 중요한 단서를 제공할 것으로 기대된다. 본 리뷰는 미생물 균총의 박테리아가 위장관암과 항암제에 어떤 영향을 미치고 있는지에 대해 초점을 맞추고 있는 논문들을 요약하고 있으며, 나아가 기존 항암제의 치료효과를 개선하기 위해 복합제로써 미생물 균총의 잠재력과 도전과제에 대해 논의한다.