• 한국수정란이식학회지
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• 제12권3호
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• pp.259-267
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• 1997

To improve the efficiency of production of cloned embryos and animals by nuclear transplantation in the rabbit, the effect of cell cycle of donor nuclei and type of recipient cytoplasm on the in vitro developmental potential and production efficiency of offspring was determined. The embryos of 16-cell stage were collected from the mated does at 48h post-hCG injection and they were synchronized to G$_1$ phase of 32-cell stage. The oocytes collected at 14h post-hCG injection were freed from cumulus cells and then enucleated. One group of the enucleated cytoplasms was activated by electrical stimulation prior to injection of donor nucleus, and the other group was not pre-activated. The separated G$_1$phase blastomeres of 32-cell stage embryos were injected into the perivitelline space of recipient cytoplasms. After culture for 20h post-hCG injection, the nuclear transplant oocytes were electrofused and activated by electrical stimulation and the fused nuclear transplant embryos were co-cultured for 120h and the nuclear transplant embryos developed to blastocyst stage were stained with Hoechst 33342 dye and their blastomeres were counted. Some of the nuclear transplant embryos developed in vitro to 2- to 4-cell stage were transferred into the oviducts of synchronized recipient does. The electrofusion rate was similar between the types of donor nuclei and recipient cytoplasms used. However, the nuclear transplant embryos using G$_1$ phase donor nuclei were developed to blastocyst at higher rate(60.3%) than those using S phase ones(24.7%). Also, when non-preactivated oocytes were used as recipient cytplasms, the develop-mental rates of nuclear transplant embryos to blastocysts were significantly(P< 0.05) higher(57.1%) than those using preactivated ones(20.8%). The cell counts of nuclear transplant embryos developed to blastosyst stage were increased signficantly(P<0.05) more in the non-preactivated recipient cytoplasm(163.7 cells), as compared whit the preactivated recipient cytoplasm(85.4 cells), A total of 49 nuclear transplant embryos were tranferrid into 5 recipient does, of which two offsprings were produced from a foster mother 31 days after embryo transfer. these results showed that the blastomeres of G1 phase and non-preactivated oocytes might be utillzed efficiently as donor nuclei and recipient cytoplasms in the nuclear transplant procedure, thought the offspring production remained still low.

• 한국치위생학회지
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• 제4권1호
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• pp.31-48
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• 2004

Dental caries taking place with milk teeth affects eruption of permanent teeth adversely, and thus, it is very important to prevent dental caries from taking place with a child in maintaining his/her physical, mental and emotional health. In this study as conducted to obtain basic data to be required for development of a program for letting preschool children form a habitual life for oral health and right dietary life, a total 172 children aged 5 years and 6 years were selected from children who lived in Hwasung-si, Kyunggi-do and attended preschools (childrens houses) located there with the consent of their guardians. Then, the weight length index (WLI) and blood components of each child were analyzed, and each relation between the weight length index (WLI) and dental caries of milk teeth and between blood components and dental caries of milk teeth was comparatively examined, and thereby, the following results were obtained. When the dmf rate of each group according to the obesity was examined, it was found to be 87.50% in the under-weight group, 71.20% in the normal weight group and 83.87% in the overweight group. And when the dmft rate of each group was examined, it was found to be 48.43% in the under-weight group, 37.10% in the normal weight group and 47.17% in the overweight group. Also, when the dmft index of each group was examined, it was found to be 3.9 in the under-weight group, 3.0 in the normal weight group and 3.8 in the overweight group. That is to say, the under-weight group and the overweight group having an abnormal weight were found to be higher in all of the dmf rate, the dmft rate and the dmft index than the normal weight group. It could be, therefore, known that normal weight should be maintained in order to prevent dental caries of milk teeth. And, it is thought that a right dietary life habit ensuring intake of balanced nutrients should be formed for maintenance of a normal weight. As a result of examining the relation between nutrient components in blood and dental caries of milk teeth, in the case of children with no dental caries, inorganic nutrient components of blood had a tendency to be a little higher, but no statistical significance was observed therein. Meanwhile, it was found that there was a relation between blood type and the dmft index.

• 한국재난정보학회 논문집
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• 제11권3호
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• pp.365-375
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• 2015

이 연구는 민간경호원을 대상으로 직장 내 소외감형성의 따돌림은 부정적 직무태도에 영향을 미치고 있지만 개인의 자아존중감에 따라 차이가 있을 것이라는 연구문제를 설정하여 직장 내 따돌림에 대한 부정적 영향을 최소화하며 궁극적으로는 조직의 응집력을 강화하고 민간경호원의 정신 및 신체적 건강을 지속적으로 유지하는데 그 결과의 의의가 있다고 볼 수 있다. 따라서 이러한 결과를 통하여 얻어진 결론은 다음과 같다. 첫째, 요인들 간의 상관관계를 분석한 결과 업무 따돌림과 인간관계 따돌림, 업무 따돌림과 이직의도, 인간관계 따돌림과 이직의도, 자기존중감과 직무만족은 서로 정적 상관관계를 가지고 있으며, 그 외의 변수들 간에는 서로 부적 상관관계를 가지고 있는 것이 나타났다. 둘째, 집단 내 소외감형성의 따돌림에 따른 자기존중감이 직무만족도에 어떠한 영향을 주는지를 알아보기 위해 회귀분석을 한 결과 연령과 업무 따돌림, 인간관계 따돌림, 자기존중감이 직무만족에 각각 영향을 주며, 연령과 자기존중감이 향상되면 직무만족은 향상되지만 직무 따돌림과 인간관계 따돌림은 감소되는 것이 나타났다. 셋째, 직장 내 집단 따돌림과 자기존중감이 이직의도에 영향을 주는지를 알아보기 위해 회귀분석을 한 결과 연령과 근속 년수, 업무 따돌림, 인간관계 따돌림, 자기존중감이 이직의도에 영향을 주며, 근속 년수와 직무따돌림, 인간관계 따돌림이 증가하게 되면 이직의도도 증가되지만, 연령과 자기존중감은 감소되는 것이 나타났다.

• Nutrition Research and Practice
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• 제12권2호
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• pp.160-165
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• 2018

BACKGROUND/OBJECTIVES: Korea is quickly becoming an aged society. Dementia is also becoming a vital public health problem in Korea. Cognitive impairment as a pre-stage of dementia shares most risk factors for dementia. The aim of the present study was to determine associations of serum levels of vitamins A, C, and E with the risk of cognitive impairment among elderly Koreans. SUBJECTS/METHODS: In this cross-sectional study, a total of 230 participants aged 60-79 years from Yangpyeong cohort were included. Cognitive function was assessed by the Korean version of the Mini-Mental State Examination for Dementia Screening. The logistic multivariable regression model was applied to determine the effect of serum vitamins A, C, and E on the risk of cognitive impairment. RESULTS: There was no significant association between the risk of cognitive impairment and serum levels of vitamin A and vitamin C. There was a significant odd ratio when the second tertile group of beta-gamma tocopherol level was compared to the first tertile group [odds ratio (OR) = 0.37, 95% confidence interval (CI) = 0.14-0.98, P for trend = 0.051]. In subgroup analyses, there were significant negative associations between beta-gamma tocopherol level and the risk of cognitive impairment in men (OR = 0.17, 95% CI = 0.03-0.87, P for trend = 0.028), non-drinkers or former drinkers (OR = 0.13, 95% CI = 0.02-0.66, P for trend = 0.025), and non-smokers or former smokers (OR = 0.27, 95% CI = 0.09-0.82, P for trend = 0.017). CONCLUSION: Serum beta-gamma tocopherol levels tended to be inversely associated with the risk of cognitive impairment. Further prospective large-scaled studies are needed to examine this association.

• 한국산학기술학회논문지
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• 제16권6호
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• pp.4040-4047
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• 2015

본 연구는 외상 후 스트레스 장애(Post-traumatic Stress Disorder; PTSD) 발생 가능성이 높은 소방관에게 단기간 음악치료 프로그램을 적용해 외상 후 스트레스(post-traumatic stress; PTS) 수준의 변화와 음악치료 효과의 지속성을 분석하고자 하였다. 연구방법은 3개 지역의 소방관 42명을 실험군(21명)과 대조군(21명)으로 임의배정 하고 5일간 1일 2회기(120분)씩 총 10회기의 음악치료를 적용하였다. PTS 수준은 음악치료 종료 직후, 4주 후, 12주 후에 변화를 측정 하였다. 연구결과 음악치료 전 PTS 수준은 $26.52{\pm}2.32$점 이었으나 음악치료 직후 $8.90{\pm}4.55$점으로 유의하게 감소하였다(p<0.05). 사후 PTS 수준은 4주 후 $11.95{\pm}4.57$점, 12주 후 $13.76{\pm}5.62$점으로 4주 및 12주 후까지 효과가 유의하게 지속되었다(p<0.05). 이상의 결과를 통해 음악치료는 PTSD 고위험군인 소방관에게 PTS 수준을 낮추는데 효과적인 접근 방법으로 판단된다. 따라서 소방관의 정신건강 관리를 위해 음악치료가 정규적인 프로그램으로 적용된다면 현장 업무능력을 향상시키는데 기여할 수 있을 것이다.

• 한국문헌정보학회지
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• 제52권1호
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• pp.341-357
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• 2018

오픈 사이언스 패러다임과 발달된 디지털 정보기술의 영향으로 여러 학문 분야에서 데이터의 공유와 재이용이 활발해지고 있으며, 데이터 중심(data intensive)의 학술 커뮤니티로 변모하고 있다. 본 논문은 사회과학 분야의 대규모 데이터 리파지토리인 Inter-university Consortium for Political and Social Research(ICPSR)에 수록된 데이터를 재이용한 저작물이 구현한 지적구조를 규명하고자 하였다. 이를 위하여 ICPSR 사이트의 2017년 발간된 데이터 재이용 저작물 570건을 분석의 대상으로 하였다. 분석의 과정은 두 단계를 거쳤다. 첫 번째 단계는 총 570건의 저작물에 대해서 저자, 저작물 형태, 저작물 자체의 주제 분석을 수행하였다. 저자를 살펴보면, 미국 대학과 연구기관 소속 연구자가 출현빈도 비중이 높은 것으로 나타났다. 저작물의 형태는 대부분은 학술지였으며, 이를 학술지 주제 분야로 분석하면, 사회과학, 의학, 심리학 분야로 나타났다. 두 번째 단계의 분석은 저작물의 제목에서 추출한 단어를 대상으로 동시출현단어 분석을 수행하여 군집과 네트워크로 시각화하였다. 이러한 결과는 보다 미시적인 주제 분야의 규명을 위해서 수행되었다. 분석결과 총 12군집인 정신건강, 담배영향, 학교/유년기/청년기장애, 청년기 성적위험, 아동부상, 육체활동, 폭력행동, 서베이, 가족역할, 여성, 문제행동, 성별차이로 구성되었음을 밝혔다. 이러한 결과를 종합적으로 살펴보면, ICPSR 데이터의 재이용을 통해 사회과학적 시각으로 의학 주제 분야의 연구가 비중있게 이루어지고 있음을 알 수 있다.

• 대한유전성대사질환학회지
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• 제3권1호
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• pp.86-93
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• 2003

Background : The SCL began screening of newborns and high risk group blood spots with tandem mass spectrometry (MS/MS) in April 2001. Our goal was to determine approximate prevalence of metabolic disorders, optimization of decision criteria for estimation of preventive effect with early diagnosis. This report describes the ongoing effort to identify more than 30 metabolic disorders by MS/MS in South Korea. Methods : Blood spot was collected from day 2 to 30 (mostly from day 2 to 10) after birth for newborn. Blood spot of high risk group was from the pediatric patients in NICU, developmental delay, mental retardation, strong family history of metabolic disorders. One punch (3.2 mm ID) of dried blood spots was extracted with $150{\mu}L$ of methanol containing isotopically labelled amino acids (AA) and acylcarnitines (AC) internal standards. Butanolic HCl was added and incubated at $65^{\circ}C$ for 15 min. The butylated extract was introduced into the inlet of MS/MS. Neutral loss of m/z 102 and parent ion mode of m/z 85 were set for the analyses of AA and AC, respectively. Diagnosis was confirmed by repeating acylcarnitine profile, urine organic acid and plasma amino acid analysis, direct enzyme assay, or molecular testing. Results : Approximately 31,000 neonates and children were screened and the estimated prevalence (newborn/high risk group), sensitivity, specificity and recall rate amounted to 1:2384/1:2066, 96.55%, 99.98%, and 0.73%, respectively. Confirmed 28 (0.09%) multiple metabolic disorders (newborn/high risk) were as follows; 13 amino acid disorders [classical PKU (3/4), BH4 deficient-hyperphenylalaninemia (0/1), Citrullinemia (1/0), Homocystinuria (0/2), Hypermethioninemia (0/1), Tyrosinemia (1/0)], 8 organic acidurias [Propionic aciduria (2/1), Methylmalonic aciduria (0/1), Isovaleric aciduria (1/1), 3-methylcrotonylglycineuria (1/0), Glutaric aciduria type1 (1/0)], 7 fatty acid oxidation disorders [LCHAD def. (2/2), Mitochondrial TFP def. (0/1), VLCAD def. (1/0), LC3KT def. (0/1). Conclnsion : The relatively normal development of 10 patients with metabolic disorders among newborns (except for the expired) demonstrates the usefulness of newborn screening by MS/MS for early diagnosis and medical intervention. However, close coordination between the MS/MS screening laboratory and the metabolic clinic/biochmical geneticists is needed to determine proper decision of screening parameters, confirmation diagnosis, follow-up scheme and additional tests.

• 한국화재소방학회논문지
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• 제25권2호
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• pp.39-46
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• 2011

다수의 거주자들이 구금되어있는 교정시설에서는 피난경로가 복잡하여 화재발생시 수용자의 도주사고 없이 안전한 대피유도가 가장 중요한 요소이다. 교정시설의 화재발생유형을 살펴보면 단기보호시설, 치료 시설에서는 의도적인 방화로 인한 화재가 많은 반면에, 교도소 및 구치소와 같은 구금시설에서는 노후한 설비 또는 화기취급부주의 등의 원인에 의해 화재가 발생한다. 두 종류의 교정시설 모두 화재가 발생하면 대피가 신속하게 이루어지지 않아 인명피해가 크다는 점에서 동일한 유형을 보인다. 본 논문에서는 교정 시설의 화재사례 및 구조적 취약성을 고려한 비상시 수용자의 대피경로 및 대피유도계획에 대해 고찰하여 화재와 같은 재난 발생 시 구금상태의 거주자를 도주사고 없이 안전하게 대피 유도하기 위해 쇠창살문에 설치된 구금장치의 전자 해정시스템의 도입을 검토하였다. 특히, 복층형 교정시설에서 보안 및 계호공백 없이 수용자의 안전한 대피유도를 실현하기 위해 피난시뮬레이션(SIMULEX)을 실행하여 산정한 필요피난소요시간(RSET)을 기반으로 구금장치의 해정시스템의 효과를 분석하였다. 결론적으로 USN(Ubiquitous Sensor Networks)기술을 활용한 전자보안출입시스템에 원격해정장치를 교정시설에 의무적으로 부착하여야만 구금상태의 수용자가 비상사태 발생 시 도주시도를 방지하면서 적절한 대피가 이루어질 수 있음을 제시하였다.

• 한국산학기술학회논문지
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• 제12권9호
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• pp.3975-3983
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• 2011

본 연구는 심장판막수술환자에서의 자가간호역량과 삶의 질 정도와 자가간호역량이 삶의 질에 미치는 요인을 규명할 목적으로 실시하였다. 연구대상은 D시 2개 대학병원에서 외래방문 치료하는 환자 110명을 대상으로 구조화된 설문지를 이용하여 설문조사를 실시하였다. 연구 결과, 심장판막수술환자의 자가간호역량과 삶의 질 정도는 총 4.0점 중 각각 평균 2.71점과 2.96점으로 그다지 높지 않은 것으로 나타났다. 또한 심장판막수술환자의 자가간호역량이 삶의 질에 미치는 영향은 신체적 기술 (${\beta}$=.625, p<.001)로 심장판막수술환자들은 자가간호역량의 신체적 기술이 높을수록 삶의 질이 높음을 알 수 있다. 결론은 자가간호역량은 심장판막 수술환자의 삶의 질에 영향을 미치는 요인으로 확인되었으며, 심장판막 수술환자는 자가간호역량이 높을수록 삶의 질이 높은 것으로 나타났다. 따라서 심장판막 수술환자의 자가간호역량을 높이기 위해 심장판막 수술환자의 신체적, 심리적, 정신적 영역에 대한 간호사정과 그에 따른 적절한 간호중재를 개발할 필요가 있다.

• Journal of Preventive Medicine and Public Health
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• 제52권6호
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• pp.345-354
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• 2019

Objectives: It is well-known that post-traumatic stress disorder (PTSD) among firefighters contributes to their job-related stress. However, the relationship between burnout and PTSD in firefighters has rarely been studied. This study therefore explored the association between burnout and its related factors, such as trauma and violence, and PTSD symptoms among firefighters in Korea. Methods: A total of 535 firefighters participated in the Firefighter Research on Enhancement of Safety & Health study at 3 university hospitals from 2016 to 2017. The 535 participants received a baseline health examination, including questionnaires assessing their mental health. A Web-based survey was also conducted to collect data on job-related stress, history of exposure to violence, burnout, and trauma experience. The associations among burnout, its related factors, and PTSD symptoms were investigated using structural equation modeling. Results: Job demands (${\beta}=0.411$, p<0.001) and effort-reward balance (${\beta}=-0.290$, p<0.001) were significantly related to burnout. Burnout (${\beta}=0.237$, p<0.001) and violence (${\beta}=0.123$, p=0.014) were significantly related to PTSD risk. Trauma (${\beta}=0.131$, p=0.001) was significantly related to burnout; however, trauma was not directly associated with PTSD scores (${\beta}=0.085$, p=0.081). Conclusions: Our results show that burnout and psychological, sexual, and physical violence at the hands of clients directly affected participants' PTSD symptoms. Burnout mediated the relationship between trauma experience and PTSD.